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The map label for this gene is hsdM

Identifier: 151221912

GI number: 151221912

Start: 1897366

End: 1899105

Strand: Reverse

Name: hsdM

Synonym: NWMN_1700

Alternate gene names: 151221912

Gene position: 1899105-1897366 (Counterclockwise)

Preceding gene: 151221913

Following gene: 151221911

Centisome position: 65.97

GC content: 37.47

Gene sequence:

>1740_bases
ATGATTTTGAAAGCATTTGAAAGCTACAACATTTCTATAAAATTTTTCAATAACAATTGCGCCACTAAAACTCAAAATTT
CCACCACCAACATCCAAATTATCAACATCGCAACATAACCAAATGTTATAATAAATCTATTACACAAAGAGATAAATTAC
TTATGCAAAGGCGGAGGAATCACATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGG
TCGATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTAGGCTTGATTTTCTATCGCTT
CTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTGTCAGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATG
AAGAATATCGTGAAGACTTAAAAGCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCG
ATGATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGCAAAGTTGAAACATCTACATT
AGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGCGATATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCA
AAGAACGTACTGCTTTAATCTCTAAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGAT
ATGTTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCAGGCGAGTTCTATACACC
ACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGTAAAGATAAATTACGTCACGTGTATGACCCAACATGTG
GTTCAGGTTCACTGTTGTTACGTGTTGGTAAAGAAACACAAGTGTATCGTTATTTCGGTCAAGAACGTAACAATACTACA
TACAACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGATATCCGTAATGATGACACATTGGA
AAACCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATTGCGAACCCACCGTATAGTGCGAAATGGACTGCAGATTCAA
AGTTTGAAAATGACGAACGATTCAGTGGTTACGGCAAACTTGCGCCTAAGTCTAAAGCAGACTTTGCCTTTATTCAACAC
ATGGTACATTACCTAGACGATGAAGGTACCATGGCCGTTGTACTCCCACATGGTGTATTATTCCGAGGTGCTGCAGAAGG
TGTCATTCGTCGTTATTTAATTGAAGAAAAGAACTACTTAGAAGCTGTGATTGGTTTGCCAGCGAATATTTTCTATGGGA
CAAGTATTCCAACATGTATTTTAGTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAAT
GATTTTGAAAAAGGAAAAAATCAAAATCATTTAAGCGATGCCCAAGTCGAACGTATTATAGACACATATAAGCGTAAGGA
AACAATTGATAAATATAGCTACAGCGCGACACTACAAGAGATTGCCGATAACGATTACAACCTAAATATACCGAGATATG
TCGATACATTCGAAGAAGAAGCACCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGATAAAGAAATCGCA
GAAATTGAGCAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA

Upstream 100 bases:

>100_bases
TGCATCAAAGCCAATCAACGATTGATTTTCACCAACTCAATTGTTGATTGGTTTTATTTATGTATGAATGAACAACTTTT
TGACATCATTAAGAATATAA

Downstream 100 bases:

>100_bases
TACACAAAAGAAAAATGTGCCAGAATTGAGATTCCCAGGATTTGAAGGCGAATGGGAAGAGAAGAAGTTAGGGAATCTTA
CTACCAAAATAGGTAGTGGA

Product: type I restriction-modification system, methyltransferase subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLW
SIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSA
MIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTT
YNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
DFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
EIEQEINAYLKELGVLKDE

Sequences:

>Translated_579_residues
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLW
SIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSA
MIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTT
YNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
DFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
EIEQEINAYLKELGVLKDE
>Mature_579_residues
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLW
SIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSA
MIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTT
YNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
DFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
EIEQEINAYLKELGVLKDE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=502, Percent_Identity=25.4980079681275, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 66949; Mature: 66949

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRN
CCEECEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHC
HMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIR
CCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
KVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
HHHHHHCCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLL
ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEE
RVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVI
EECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEE
ANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVL
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHH
FRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCC
DFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEE
CHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
APIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRN
CCEECEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHC
HMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIR
CCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
KVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
HHHHHHCCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLL
ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEE
RVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAVI
EECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEE
ANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVL
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHH
FRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCC
DFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEE
CHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
APIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]