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The map label for this gene is thrA

Identifier: 151221451

GI number: 151221451

Start: 1361802

End: 1363196

Strand: Reverse

Name: thrA

Synonym: NWMN_1239

Alternate gene names: 151221451

Gene position: 1363196-1361802 (Counterclockwise)

Preceding gene: 151221456

Following gene: 151221449

Centisome position: 47.35

GC content: 31.61

Gene sequence:

>1395_bases
TTGGCATGCACTATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTTTTAAATAT
TGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAAAGACATGATAATGATATTAAAACAACTG
ATTTGTTAATCAGATTATATGAAAAGGTCATTAATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTAT
GATGATATTGTAAAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTATATTAATCA
ATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAAGATTTTAATGCGCAATTGATAGCTTTAT
ATAATAATAGTCAAGGTATACCAACAAAATATATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAA
GCACAAATATTAGATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAACTAATTATCCCAGGATTTTT
TGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGATATAACTGGCGCCATCATATCAAGTGGTG
TTAGAGCCACAATTTATGAAAATTTCACCGATGTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAA
TTAATCGAAGAAATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAAGCCTTACA
ACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCTCAAGATAAGGGTACATTTATAGTGCATG
ATAGAGAAATAAATGCGAAAAATGTGATTAGCGGTATAAGTTGTGATAAAGATTTTACCGTTATTAATATCAAAAAGTAT
TTAATGAATAGACAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGATCATATGCC
TTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAATAAAGAAACAAAAGTATTAAATGAAATTC
GTCAAAAATGTGATGTTGATGAGCTAAGTATTGAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAA
GTCATCGGTACTGCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAAGGTGCTTC
GGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTATTATCTACATATGAGTTTTGCTATCACG
GGGTTGCATTAAAAAACTTGTGTGATAAAAACTAA

Upstream 100 bases:

>100_bases
TATTCTTTATTTTTGTAAAGTTCGTAATATTCAAAATATAAATACATAATATCATTCTTACTTACAAAACTGAATACAAT
TTTCTGATTTTTTAGAAAGG

Downstream 100 bases:

>100_bases
TATAAAAGCAATTCGTAACACATGTATTCAACTGCGTTATGAATTGCTTTTTCTTAATTATTTATTGTTCTCTGGATTGC
ATCCTTAATACGTTGCTCAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MACTMKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRY
DDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQE
AQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPE
LIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKY
LMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNK
VIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Sequences:

>Translated_464_residues
MACTMKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRY
DDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQE
AQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPE
LIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKY
LMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNK
VIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
>Mature_463_residues
ACTMKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYD
DIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEA
QILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPEL
IEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYL
MNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKV
IGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=472, Percent_Identity=26.9067796610169, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=25.1091703056769, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790376, Length=302, Percent_Identity=25.4966887417219, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=486, Percent_Identity=24.8971193415638, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 52411; Mature: 52280

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACTMKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLY
CCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
EKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEK
HCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCE
LIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPE
EEECCEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEHHCCCCCCCEEECCCCCCCCHH
LIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCHH
NVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSK
HHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
QIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLK
HHCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHCCCCEE
MINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
EECCCHHHEEHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ACTMKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLY
CEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
EKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEK
HCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCE
LIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPE
EEECCEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEHHCCCCCCCEEECCCCCCCCHH
LIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCHH
NVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSK
HHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
QIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLK
HHCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHCCCCEE
MINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
EECCCHHHEEHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]