Click here to switch to the map view.

The map label for this gene is thiD

Identifier: 151220755

GI number: 151220755

Start: 627641

End: 628471

Strand: Reverse

Name: thiD

Synonym: NWMN_0543

Alternate gene names: 151220755

Gene position: 628471-627641 (Counterclockwise)

Preceding gene: 151220760

Following gene: 151220754

Centisome position: 21.83

GC content: 38.03

Gene sequence:

>831_bases
ATGGCTTTAAAGAAAGTTTTAACAATTGCCGGTTCTGACACAAGTGCTGGCGCAGGTATGCAAGCAGATTTGAAAACGTT
CCAAGAATTAGATACGTATGGCATGGTCGCTTTAACTGCCATCGTTACTATGGATAAAGATACATGGTCACACGATGTTA
CACCATTACCAATGGATGTATTTGAAAAACAACTTGAAACGGCTTTATCAATCGGACCTGATGCTATTAAAACAGGTATG
TTAGGTACTGAGGAGATAATCAAACGTGCTGGAGAAGTATATGAGGCATCTAATGCACAATATTTTGTAGTAGATCCTGT
CATGGTTTGTAAAGGCGAAGATGAAGTGCTTAATCCTGGAAATACTGAGGCGATGATTAAATATTTACTACCTAAAGCAA
CGGTAGTGGCACCAAACTTATTTGAAGCGGGTCAATTATCAGGATTAGGTAAATTAAATTCAATTGAAGATATGAAAAAG
GCTGCAACAATTATCTTTGATAAAGGCGCACAGCATGTCATCATTAAAGGTGGCAAAGCTTTAGATCAAGATAAATCGTA
TGACTTATACTATGATGGTCAAACATTTTATCAACTAACAACAGACATGTTCCAACAAAGTTATAACCATGGTGCTGGTT
GTACATTTGCTGCGGCAACAACTGCATATTTAGCTAACGGTAAGTCACCGAAAGAAGCTGTGATTAGCGCGAAAGCTTTC
GTCGCTTCTGCTATTAAAAACGGTTGGAAAATGAATGATTTTGTTGGTCCTGTGGATCACGGTGCATACAACCGTATTGA
ACATATCGATGTTGAAGTAACAGAGGTTTAA

Upstream 100 bases:

>100_bases
ATTCATTTCAACAATCATCAAATATTGAATGCTTAATATTTTTCAATTAGGTTAAAAATGATATGATGAATACAATATTA
TTATGTAGGAGTGAAGCTCA

Downstream 100 bases:

>100_bases
CGATTAGGCGGTTCATCTATAAATCAACATTATTAGCCACACCACTTAAATTGGGTATGAACTCAATTTATGTGATGTGG
TTATTTTTTTATAATCATCA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGM
LGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVAPNLFEAGQLSGLGKLNSIEDMKK
AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV

Sequences:

>Translated_276_residues
MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGM
LGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVAPNLFEAGQLSGLGKLNSIEDMKK
AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
>Mature_275_residues
ALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGML
GTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVAPNLFEAGQLSGLGKLNSIEDMKKA
ATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFV
ASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=31.1787072243346, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325378, Length=275, Percent_Identity=28, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=28.8321167883212, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=27.9411764705882, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 29827; Mature: 29696

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDV
CCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
FEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPG
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHEECCCCEEEEECCEEEECCCCCCCCCC
NTEAMIKYLLPKATVVAPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKA
CHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCEEEEECCCC
LDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
ALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDV
CHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
FEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPG
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHEECCCCEEEEECCEEEECCCCCCCCCC
NTEAMIKYLLPKATVVAPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKA
CHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCEEEEECCCC
LDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA