Click here to switch to the map view.

The map label for this gene is fen

Identifier: 150401914

GI number: 150401914

Start: 1553355

End: 1554335

Strand: Reverse

Name: fen

Synonym: Maeo_1492

Alternate gene names: 150401914

Gene position: 1554335-1553355 (Counterclockwise)

Preceding gene: 150401915

Following gene: 150401913

Centisome position: 99.03

GC content: 28.24

Gene sequence:

>981_bases
ATGGGAGTGCAATTTAATGACTCAATTCCAAAAAAGAACATATCAATAACAGATTTAAAAAATAAAACCGTGGCAATTGA
TTCAATGAATATAATATATCAATTTTTATCAAGTATTAGATTAAGAGATGGAGCTCCGTTAAGAAATTCAAAGGGGGAAA
TAACATCACCATATAATGGAATATTTTATAAAACAATATATCTATTGAATAATGAAATAACTCCCATATGGGTATTTGAT
GGAAAACCGCCAGAATTAAAACTTAAAACACGAGAAGAAAGAAGAAAAGTAAAAGAAAAAGCATCAAAGGACTACGAAAT
AGCAAAAAGAGAAGAAAACATTGAAGATATGCAAAAATATGCAAAAAGAATTAATTATTTAGAACCAAATACAGTTGATA
ACTGCAAAAAATTATTAAAACTCATGGGAATCCCATATATAGATGCCCCATCAGAGGGGGAAGCCCAATGTGCCCATATG
ATAAAAAACGGCGATGCCTACTGTGTAGTTAGTCAAGATTATGATGCCTTATTATATGGAGCTCCGAGAACTGTAAGAAA
TATTACGGCATCAAATAAACCATTGGAACTGATGGAAATTGAGGACATATTAAAACCATTGGATATATCTATTGATGATT
TAATTGATATGGCAATATTAATTGGGACCGACTATAATATTGGAGGGATTAAAGGAATAGGCCCTAAAAAAGCATTAACT
ATAATAAAAAATAAAAAAATGAATGAATATATAAAAGATATTGAAAATTATGAGGAGATAAAAAATATATTTAAAAATCC
AAAAGTCGTGGATTATACGAAAGAGGACATAAAATTAAAATCTCCAAATATTGAAGGATTAAAAGAATTTTTGATAGAAG
AAAATGATTTTTCACCAAATAGAATTTTGCCATCAATTAAAAAATTGGATAAATTATTAAATGAGAAAAGAAGCCAAACT
TCATTGGATAGTTGGTTTTAA

Upstream 100 bases:

>100_bases
GTTTAATAGCCCCTCCATTGTTAATAAATGGAAAGATAATTGTTAATTAAATATTTTTTTTATTTTATAATAAATAATAA
TAAATATTTTGGTGAATAAA

Downstream 100 bases:

>100_bases
AGGAAAAAATAAGAATAAAATAATAATTAAAGAATAAATAAAATTTAAAAATTTAAAAAAGTGATAACATGGTTAAACTT
AAAACATACAATTTAACAAA

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGVQFNDSIPKKNISITDLKNKTVAIDSMNIIYQFLSSIRLRDGAPLRNSKGEITSPYNGIFYKTIYLLNNEITPIWVFD
GKPPELKLKTREERRKVKEKASKDYEIAKREENIEDMQKYAKRINYLEPNTVDNCKKLLKLMGIPYIDAPSEGEAQCAHM
IKNGDAYCVVSQDYDALLYGAPRTVRNITASNKPLELMEIEDILKPLDISIDDLIDMAILIGTDYNIGGIKGIGPKKALT
IIKNKKMNEYIKDIENYEEIKNIFKNPKVVDYTKEDIKLKSPNIEGLKEFLIEENDFSPNRILPSIKKLDKLLNEKRSQT
SLDSWF

Sequences:

>Translated_326_residues
MGVQFNDSIPKKNISITDLKNKTVAIDSMNIIYQFLSSIRLRDGAPLRNSKGEITSPYNGIFYKTIYLLNNEITPIWVFD
GKPPELKLKTREERRKVKEKASKDYEIAKREENIEDMQKYAKRINYLEPNTVDNCKKLLKLMGIPYIDAPSEGEAQCAHM
IKNGDAYCVVSQDYDALLYGAPRTVRNITASNKPLELMEIEDILKPLDISIDDLIDMAILIGTDYNIGGIKGIGPKKALT
IIKNKKMNEYIKDIENYEEIKNIFKNPKVVDYTKEDIKLKSPNIEGLKEFLIEENDFSPNRILPSIKKLDKLLNEKRSQT
SLDSWF
>Mature_325_residues
GVQFNDSIPKKNISITDLKNKTVAIDSMNIIYQFLSSIRLRDGAPLRNSKGEITSPYNGIFYKTIYLLNNEITPIWVFDG
KPPELKLKTREERRKVKEKASKDYEIAKREENIEDMQKYAKRINYLEPNTVDNCKKLLKLMGIPYIDAPSEGEAQCAHMI
KNGDAYCVVSQDYDALLYGAPRTVRNITASNKPLELMEIEDILKPLDISIDDLIDMAILIGTDYNIGGIKGIGPKKALTI
IKNKKMNEYIKDIENYEEIKNIFKNPKVVDYTKEDIKLKSPNIEGLKEFLIEENDFSPNRILPSIKKLDKLLNEKRSQTS
LDSWF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=323, Percent_Identity=38.6996904024768, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI194018535, Length=252, Percent_Identity=24.6031746031746, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI194018531, Length=252, Percent_Identity=24.6031746031746, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI51988900, Length=226, Percent_Identity=26.9911504424779, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17510005, Length=325, Percent_Identity=35.0769230769231, Blast_Score=203, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6322736, Length=333, Percent_Identity=35.7357357357357, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321697, Length=108, Percent_Identity=35.1851851851852, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320469, Length=261, Percent_Identity=26.8199233716475, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17647423, Length=323, Percent_Identity=38.390092879257, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24658219, Length=230, Percent_Identity=30, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI78706854, Length=109, Percent_Identity=38.5321100917431, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI78706850, Length=109, Percent_Identity=38.5321100917431, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI78706852, Length=109, Percent_Identity=38.5321100917431, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137168, Length=264, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_META3 (A6UX46)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325680.1
- ProteinModelPortal:   A6UX46
- SMR:   A6UX46
- STRING:   A6UX46
- GeneID:   5326506
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1492
- eggNOG:   arNOG04505
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- ProtClustDB:   PRK03980
- BioCyc:   MAEO419665:MAEO_1492-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 37437; Mature: 37306

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVQFNDSIPKKNISITDLKNKTVAIDSMNIIYQFLSSIRLRDGAPLRNSKGEITSPYNG
CCCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
IFYKTIYLLNNEITPIWVFDGKPPELKLKTREERRKVKEKASKDYEIAKREENIEDMQKY
CEEEEEEEECCCCCEEEEECCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHH
AKRINYLEPNTVDNCKKLLKLMGIPYIDAPSEGEAQCAHMIKNGDAYCVVSQDYDALLYG
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEC
APRTVRNITASNKPLELMEIEDILKPLDISIDDLIDMAILIGTDYNIGGIKGIGPKKALT
CCHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHH
IIKNKKMNEYIKDIENYEEIKNIFKNPKVVDYTKEDIKLKSPNIEGLKEFLIEENDFSPN
HHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCHHHHHHHHHCCCCCCCH
RILPSIKKLDKLLNEKRSQTSLDSWF
HHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
GVQFNDSIPKKNISITDLKNKTVAIDSMNIIYQFLSSIRLRDGAPLRNSKGEITSPYNG
CCCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
IFYKTIYLLNNEITPIWVFDGKPPELKLKTREERRKVKEKASKDYEIAKREENIEDMQKY
CEEEEEEEECCCCCEEEEECCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHH
AKRINYLEPNTVDNCKKLLKLMGIPYIDAPSEGEAQCAHMIKNGDAYCVVSQDYDALLYG
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEC
APRTVRNITASNKPLELMEIEDILKPLDISIDDLIDMAILIGTDYNIGGIKGIGPKKALT
CCHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCHHHHHH
IIKNKKMNEYIKDIENYEEIKNIFKNPKVVDYTKEDIKLKSPNIEGLKEFLIEENDFSPN
HHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCHHHHHHHHHCCCCCCCH
RILPSIKKLDKLLNEKRSQTSLDSWF
HHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA