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The map label for this gene is rfcL

Identifier: 150401783

GI number: 150401783

Start: 1422733

End: 1424157

Strand: Reverse

Name: rfcL

Synonym: Maeo_1361

Alternate gene names: 150401783

Gene position: 1424157-1422733 (Counterclockwise)

Preceding gene: 150401785

Following gene: 150401773

Centisome position: 90.74

GC content: 30.32

Gene sequence:

>1425_bases
ATGAATTGGGTTGAAAAATACCGACCAAAAACAATGTCAGAAATTGTTGGAAATAATAAAATTAAAGAAGAACTAAAAAA
CTGGATAGAAGAAATATTGCATAATGAAATCCCAAAACCTGTTTTATTGGTTGGTCCGCCAGGATGTGGTAAAACCACAC
TTACTAATGCCCTTGCAAATGATTATGGATTTGAATTAATTGAATTAAATGCAAGTGATAAAAGAAATAAAGATATAATC
CGCCAAATTGTGGGCGGTGCCTCTTCCTCAAAATCCATAACAGGTAAAAGAGTATTAATAGTATTAGATGAAGTTGACGG
ACTTTCTGGAAACAGTGACAGGGGAGGAGTTAGCGAAATAATAAAAATAATAAAAAATGCAAAAAATCCAATAATTTTAA
CTGCAAATGATATATATAAACCATCTTTGATGTCTTTAAGAACCGTATGTAATATTGTAAAAATCGGTTCAGTTCATACA
AATTCCATTGTTCCAGTATTGCGGAGAATATCTTTAAAAGAAGGCTTTGAAGTAGATGACAAAATATTAAAAATTATAGC
AAAACATGCAGGGGGAGATGTTAGAAGTGCCATTAACGACTTGGAATCTCTTGCTATGGGGAATACTTTCGATGAAGATA
ATGTAAGGGAGCTCCCTGACAGAGACACAGATAAAAGTATTTTTGATGCCATAAGAATTATATTTAAAACTACCCACTAC
GATATATCATTAGAAGCTACACGAGATTTAAAAGAGGATATAGGAACGGTTCAGGAATGGATAGCCGAAAATGTGCCAAG
AGAATATAAAAAAGGAAAAGAAATATGTTTAGCATATGATAATTTATCAAAAGCAGATATATTTTTAGGCAGAGTGTATA
AAAGGCAGTATTATGGGCTTTGGAGATATGCTTCGGCATTAATGAGTGCAGGAGTATCATTGGCAAAAGATGAAAAATAT
AGGGGATTTTTTTCATATATGAGACCTACAATATTTACAAAAATGTCAAGAAGTAAGGGAAAACGGGGGGCAGTGAATAA
AGTGCTTGAAAAAATAAGTTTAAAATCTCATGTATCAAAAAGAAGGGCGCGGGAGGATATTTTATATTTAGAATATATTT
TAAAAAATGATGCCGAAATGGGGGCAAAATTATGCGAATTTTACGAATTTACAAAAGAAGATATTGAATATTTAACAAAC
AAAACAATTGCCAAAAAAATAATTAAATTAATGAAGGGAGATGATAAAAAAACCAATAATAAAAAAGGAAAAGAAAATAA
AACCAAAAATACCACTAAAAAGATTAAAGAAATTAAAGAAACTCCAAAAAAAGAAGAAGTTAAAGAACCTAAAAAACAAA
TAGAAAAACAAAAATCAGAGAAAAAAGAACCTAAAAAACAAATGACATTGGAATCATTTTTCTAA

Upstream 100 bases:

>100_bases
CAGTAGAGTTATTTAATGATATAAAACGATATAAAACGATAAAAACTATTATGGCATTCAAAAACAATTTTTTGAACATA
CTACAATAAATGGTGATTTA

Downstream 100 bases:

>100_bases
TTTTATAAATTTTTTGGGAGCTCCCATATAAAATATATAAAAAAATAAATTAAATTCCCATTAAGGCCAATATAACTCCC
ACAAAGGCTATAATGGTTGT

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNWVEKYRPKTMSEIVGNNKIKEELKNWIEEILHNEIPKPVLLVGPPGCGKTTLTNALANDYGFELIELNASDKRNKDII
RQIVGGASSSKSITGKRVLIVLDEVDGLSGNSDRGGVSEIIKIIKNAKNPIILTANDIYKPSLMSLRTVCNIVKIGSVHT
NSIVPVLRRISLKEGFEVDDKILKIIAKHAGGDVRSAINDLESLAMGNTFDEDNVRELPDRDTDKSIFDAIRIIFKTTHY
DISLEATRDLKEDIGTVQEWIAENVPREYKKGKEICLAYDNLSKADIFLGRVYKRQYYGLWRYASALMSAGVSLAKDEKY
RGFFSYMRPTIFTKMSRSKGKRGAVNKVLEKISLKSHVSKRRAREDILYLEYILKNDAEMGAKLCEFYEFTKEDIEYLTN
KTIAKKIIKLMKGDDKKTNNKKGKENKTKNTTKKIKEIKETPKKEEVKEPKKQIEKQKSEKKEPKKQMTLESFF

Sequences:

>Translated_474_residues
MNWVEKYRPKTMSEIVGNNKIKEELKNWIEEILHNEIPKPVLLVGPPGCGKTTLTNALANDYGFELIELNASDKRNKDII
RQIVGGASSSKSITGKRVLIVLDEVDGLSGNSDRGGVSEIIKIIKNAKNPIILTANDIYKPSLMSLRTVCNIVKIGSVHT
NSIVPVLRRISLKEGFEVDDKILKIIAKHAGGDVRSAINDLESLAMGNTFDEDNVRELPDRDTDKSIFDAIRIIFKTTHY
DISLEATRDLKEDIGTVQEWIAENVPREYKKGKEICLAYDNLSKADIFLGRVYKRQYYGLWRYASALMSAGVSLAKDEKY
RGFFSYMRPTIFTKMSRSKGKRGAVNKVLEKISLKSHVSKRRAREDILYLEYILKNDAEMGAKLCEFYEFTKEDIEYLTN
KTIAKKIIKLMKGDDKKTNNKKGKENKTKNTTKKIKEIKETPKKEEVKEPKKQIEKQKSEKKEPKKQMTLESFF
>Mature_474_residues
MNWVEKYRPKTMSEIVGNNKIKEELKNWIEEILHNEIPKPVLLVGPPGCGKTTLTNALANDYGFELIELNASDKRNKDII
RQIVGGASSSKSITGKRVLIVLDEVDGLSGNSDRGGVSEIIKIIKNAKNPIILTANDIYKPSLMSLRTVCNIVKIGSVHT
NSIVPVLRRISLKEGFEVDDKILKIIAKHAGGDVRSAINDLESLAMGNTFDEDNVRELPDRDTDKSIFDAIRIIFKTTHY
DISLEATRDLKEDIGTVQEWIAENVPREYKKGKEICLAYDNLSKADIFLGRVYKRQYYGLWRYASALMSAGVSLAKDEKY
RGFFSYMRPTIFTKMSRSKGKRGAVNKVLEKISLKSHVSKRRAREDILYLEYILKNDAEMGAKLCEFYEFTKEDIEYLTN
KTIAKKIIKLMKGDDKKTNNKKGKENKTKNTTKKIKEIKETPKKEEVKEPKKQIEKQKSEKKEPKKQMTLESFF

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=423, Percent_Identity=27.8959810874704, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI6677723, Length=307, Percent_Identity=26.3843648208469, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI194306571, Length=305, Percent_Identity=26.2295081967213, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI194306567, Length=305, Percent_Identity=26.2295081967213, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI31563534, Length=266, Percent_Identity=28.1954887218045, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI4506491, Length=216, Percent_Identity=28.2407407407407, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI31881687, Length=216, Percent_Identity=28.2407407407407, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17563226, Length=246, Percent_Identity=32.520325203252, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17541988, Length=255, Percent_Identity=29.0196078431373, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17541368, Length=239, Percent_Identity=26.7782426778243, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71986063, Length=256, Percent_Identity=29.296875, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554730, Length=246, Percent_Identity=29.6747967479675, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324791, Length=225, Percent_Identity=29.3333333333333, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323724, Length=303, Percent_Identity=26.0726072607261, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324039, Length=225, Percent_Identity=28.4444444444444, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324478, Length=230, Percent_Identity=29.5652173913043, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322528, Length=228, Percent_Identity=28.5087719298246, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737765, Length=455, Percent_Identity=25.7142857142857, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI62472391, Length=477, Percent_Identity=25.1572327044025, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574716, Length=244, Percent_Identity=28.6885245901639, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921076, Length=223, Percent_Identity=29.1479820627803, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18859927, Length=215, Percent_Identity=26.9767441860465, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17647857, Length=216, Percent_Identity=25.9259259259259, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_META3 (A6UWR5)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325549.1
- ProteinModelPortal:   A6UWR5
- SMR:   A6UWR5
- STRING:   A6UWR5
- GeneID:   5326564
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1361
- eggNOG:   arNOG04036
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- ProtClustDB:   PRK04195
- BioCyc:   MAEO419665:MAEO_1361-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: 3.4.24.- [C]

Molecular weight: Translated: 54073; Mature: 54073

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWVEKYRPKTMSEIVGNNKIKEELKNWIEEILHNEIPKPVLLVGPPGCGKTTLTNALAN
CCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
DYGFELIELNASDKRNKDIIRQIVGGASSSKSITGKRVLIVLDEVDGLSGNSDRGGVSEI
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHH
IKIIKNAKNPIILTANDIYKPSLMSLRTVCNIVKIGSVHTNSIVPVLRRISLKEGFEVDD
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHH
KILKIIAKHAGGDVRSAINDLESLAMGNTFDEDNVRELPDRDTDKSIFDAIRIIFKTTHY
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCC
DISLEATRDLKEDIGTVQEWIAENVPREYKKGKEICLAYDNLSKADIFLGRVYKRQYYGL
EEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
WRYASALMSAGVSLAKDEKYRGFFSYMRPTIFTKMSRSKGKRGAVNKVLEKISLKSHVSK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RRAREDILYLEYILKNDAEMGAKLCEFYEFTKEDIEYLTNKTIAKKIIKLMKGDDKKTNN
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KKGKENKTKNTTKKIKEIKETPKKEEVKEPKKQIEKQKSEKKEPKKQMTLESFF
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC
>Mature Secondary Structure
MNWVEKYRPKTMSEIVGNNKIKEELKNWIEEILHNEIPKPVLLVGPPGCGKTTLTNALAN
CCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
DYGFELIELNASDKRNKDIIRQIVGGASSSKSITGKRVLIVLDEVDGLSGNSDRGGVSEI
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHH
IKIIKNAKNPIILTANDIYKPSLMSLRTVCNIVKIGSVHTNSIVPVLRRISLKEGFEVDD
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHH
KILKIIAKHAGGDVRSAINDLESLAMGNTFDEDNVRELPDRDTDKSIFDAIRIIFKTTHY
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCC
DISLEATRDLKEDIGTVQEWIAENVPREYKKGKEICLAYDNLSKADIFLGRVYKRQYYGL
EEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
WRYASALMSAGVSLAKDEKYRGFFSYMRPTIFTKMSRSKGKRGAVNKVLEKISLKSHVSK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RRAREDILYLEYILKNDAEMGAKLCEFYEFTKEDIEYLTNKTIAKKIIKLMKGDDKKTNN
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KKGKENKTKNTTKKIKEIKETPKKEEVKEPKKQIEKQKSEKKEPKKQMTLESFF
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA