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The map label for this gene is truB

Identifier: 150401644

GI number: 150401644

Start: 1291062

End: 1292057

Strand: Reverse

Name: truB

Synonym: Maeo_1222

Alternate gene names: 150401644

Gene position: 1292057-1291062 (Counterclockwise)

Preceding gene: 150401645

Following gene: 150401643

Centisome position: 82.32

GC content: 34.14

Gene sequence:

>996_bases
TTGGAATTAATTACAAAGGAAGAATCAAAGACAAATTATGAATATGGTTGCGACCCATACAATAGACCAATAGGGGAGCT
CCTTAAAAATGGTATTGTTGTAATAGATAAACCATCGGGACCAACTTCCCATGAGGTATCTGCATGGGTTAGAGATATAT
TAAAGCTAAAACTTGCAGGGCATGGCGGAACACTTGACCCAAAAGTGACAGGCGTATTGCCTGTTGCACTGGAAAACACT
TCAAAATGTATATCTGTGTGGCATATGATTCCTAAGGAGTATGTTTGTTTAATGCACTTACATAGAGATACGGAAGAGGA
GGAATTATTAAAAATATTTAAAAAATTCACAGGCAGGATATTTCAAAGACCGCCTTTAAAAGCCGCCGTAAAAAGAAGTT
TAAGAATTAGGAAAATTTACGAATTAGAATTGCTTGAAAAAGACGGAAGAGATGTATTATTTAGAGTAAGATGTCAATCC
GGAACATACATAAGAACACTTGCCGAAGATATGGGGGAAGCACTTGGAACTTCGGCACATATGCAGGAGCTCCGAAGAAT
TGTAAGTGGACCATTCACAGAAAAAGATATAGTTTATTTGCAGGATTTAACAGATGCATATATTTTTTGGAAAGAAAACG
GGGACGAAACAGAACTAAGAAAAATAATAAAACCTATGGAATATGGACTTCAACATATCAATAAAATAATTGTTAAAGAT
AGTGCAGTAGATGCTATATGCCATGGTGCAAATGTATATTTAAACGGAGTATCCAAATTAAGTAAAGGAATAGGGGCAGA
CGAAACCGTATTAATTGAAACATTAAAAGGGGAGGCAATAGGAATAGGAACGGCACTATTAAACACCAAAAATGTATTAA
AAGAAAGTAAAAACGAAGAAGATAATAGGGAAAAATATAAAGAACCTATTGTAGATATTGAAAGGATTTTTATGAGTCCA
AATACATATCCTAAAATGTGGAAAAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GGATTTTTAAAATTTGTAAAACTGATTAAAAATAAAAAAATTAAAAAAGTATAAAATAAAGAACTTGTATAAAATAAATA
TAAAATATTTGGTGGTATTT

Downstream 100 bases:

>100_bases
TAAATAATTATGAGGGGTGGAGTTATGGGACTCCAAGCAATGCATTTAATCGTATTATTTATTTTTTTATTAATTGGATT
GGAATTAATCGGGCAGTTAA

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MELITKEESKTNYEYGCDPYNRPIGELLKNGIVVIDKPSGPTSHEVSAWVRDILKLKLAGHGGTLDPKVTGVLPVALENT
SKCISVWHMIPKEYVCLMHLHRDTEEEELLKIFKKFTGRIFQRPPLKAAVKRSLRIRKIYELELLEKDGRDVLFRVRCQS
GTYIRTLAEDMGEALGTSAHMQELRRIVSGPFTEKDIVYLQDLTDAYIFWKENGDETELRKIIKPMEYGLQHINKIIVKD
SAVDAICHGANVYLNGVSKLSKGIGADETVLIETLKGEAIGIGTALLNTKNVLKESKNEEDNREKYKEPIVDIERIFMSP
NTYPKMWKKKK

Sequences:

>Translated_331_residues
MELITKEESKTNYEYGCDPYNRPIGELLKNGIVVIDKPSGPTSHEVSAWVRDILKLKLAGHGGTLDPKVTGVLPVALENT
SKCISVWHMIPKEYVCLMHLHRDTEEEELLKIFKKFTGRIFQRPPLKAAVKRSLRIRKIYELELLEKDGRDVLFRVRCQS
GTYIRTLAEDMGEALGTSAHMQELRRIVSGPFTEKDIVYLQDLTDAYIFWKENGDETELRKIIKPMEYGLQHINKIIVKD
SAVDAICHGANVYLNGVSKLSKGIGADETVLIETLKGEAIGIGTALLNTKNVLKESKNEEDNREKYKEPIVDIERIFMSP
NTYPKMWKKKK
>Mature_331_residues
MELITKEESKTNYEYGCDPYNRPIGELLKNGIVVIDKPSGPTSHEVSAWVRDILKLKLAGHGGTLDPKVTGVLPVALENT
SKCISVWHMIPKEYVCLMHLHRDTEEEELLKIFKKFTGRIFQRPPLKAAVKRSLRIRKIYELELLEKDGRDVLFRVRCQS
GTYIRTLAEDMGEALGTSAHMQELRRIVSGPFTEKDIVYLQDLTDAYIFWKENGDETELRKIIKPMEYGLQHINKIIVKD
SAVDAICHGANVYLNGVSKLSKGIGADETVLIETLKGEAIGIGTALLNTKNVLKESKNEEDNREKYKEPIVDIERIFMSP
NTYPKMWKKKK

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI4503337, Length=316, Percent_Identity=41.1392405063291, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI215599015, Length=321, Percent_Identity=40.809968847352, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI21040257, Length=216, Percent_Identity=27.3148148148148, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI2367200, Length=208, Percent_Identity=29.3269230769231, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17553978, Length=316, Percent_Identity=36.3924050632911, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6323204, Length=331, Percent_Identity=39.5770392749245, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI281364189, Length=321, Percent_Identity=40.1869158878505, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI281364187, Length=321, Percent_Identity=40.1869158878505, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI281364185, Length=321, Percent_Identity=40.1869158878505, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI281364183, Length=321, Percent_Identity=40.1869158878505, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI62471759, Length=321, Percent_Identity=40.1869158878505, Blast_Score=240, Evalue=8e-64,
Organism=Drosophila melanogaster, GI17975520, Length=321, Percent_Identity=40.1869158878505, Blast_Score=240, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_META3 (A6UWC6)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325410.1
- ProteinModelPortal:   A6UWC6
- SMR:   A6UWC6
- STRING:   A6UWC6
- GeneID:   5327489
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1222
- eggNOG:   arNOG04654
- HOGENOM:   HBG397258
- OMA:   SGPTSHE
- ProtClustDB:   PRK04270
- BioCyc:   MAEO419665:MAEO_1222-MONOMER
- HAMAP:   MF_01081
- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- SMART:   SM00359
- TIGRFAMs:   TIGR00425

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 37717; Mature: 37717

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELITKEESKTNYEYGCDPYNRPIGELLKNGIVVIDKPSGPTSHEVSAWVRDILKLKLAG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCC
HGGTLDPKVTGVLPVALENTSKCISVWHMIPKEYVCLMHLHRDTEEEELLKIFKKFTGRI
CCCCCCCCHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FQRPPLKAAVKRSLRIRKIYELELLEKDGRDVLFRVRCQSGTYIRTLAEDMGEALGTSAH
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHH
MQELRRIVSGPFTEKDIVYLQDLTDAYIFWKENGDETELRKIIKPMEYGLQHINKIIVKD
HHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SAVDAICHGANVYLNGVSKLSKGIGADETVLIETLKGEAIGIGTALLNTKNVLKESKNEE
HHHHHHHCCCHHEEHHHHHHHHCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCC
DNREKYKEPIVDIERIFMSPNTYPKMWKKKK
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MELITKEESKTNYEYGCDPYNRPIGELLKNGIVVIDKPSGPTSHEVSAWVRDILKLKLAG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCC
HGGTLDPKVTGVLPVALENTSKCISVWHMIPKEYVCLMHLHRDTEEEELLKIFKKFTGRI
CCCCCCCCHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FQRPPLKAAVKRSLRIRKIYELELLEKDGRDVLFRVRCQSGTYIRTLAEDMGEALGTSAH
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCHHH
MQELRRIVSGPFTEKDIVYLQDLTDAYIFWKENGDETELRKIIKPMEYGLQHINKIIVKD
HHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SAVDAICHGANVYLNGVSKLSKGIGADETVLIETLKGEAIGIGTALLNTKNVLKESKNEE
HHHHHHHCCCHHEEHHHHHHHHCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCC
DNREKYKEPIVDIERIFMSPNTYPKMWKKKK
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA