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The map label for this gene is lpdA [C]

Identifier: 150401511

GI number: 150401511

Start: 1137952

End: 1139235

Strand: Direct

Name: lpdA [C]

Synonym: Maeo_1088

Alternate gene names: 150401511

Gene position: 1137952-1139235 (Clockwise)

Preceding gene: 150401508

Following gene: 150401512

Centisome position: 72.5

GC content: 28.74

Gene sequence:

>1284_bases
ATGGAACTTCAAAATTTTTCGGAAAAAAATTTTTACAGTCGTAAAAATAGCAAAGCTATTTTGAGACCCCCTGAAAATAC
AAAAAATATTGAAAATACAAAAATTGCCGTTATTGGTGGCGGACCTGCTGGAAAATATTGCAGCACCGAATTAGCCAAAA
AAGGATTTTCCGTATCAATTTACGAAAAAAACAAAATAGGAGGCACCTGTTTAAATTACGGTTGCACATATGTAACCGGA
TTACGGGAAATGGCAGATATAATAAATAATTTAAATATAGTTAACTCAAATAAAGAAATAAAAGATAATACAAAATTAGA
AGATGTTATTTCATTTAAAGAACTTCAAAAAAATATATCGAATATTCTAACTAAAATAAGAGGAAAATTAGAGGGGGACC
TATTAAAATACAATAATATAAAATTATATAATGAAGAATTTAAAGAAGAATATAAAAAAAATTATGACTATATTGTATAT
GCAACGGGTAAAGAATATACAAATAATTATAATGGTGTGGAATGCCTAATACATTCGGATATTGTAAATTTAGAGGAGCT
CCCTGAAAAGATATTAATTGTGGGCGGGGGAACTGTGGCAATGGAATATGCTTCGCTGTTTTCAAATTATGGCTGTGAAG
TGGTTGTTTATGTTCGTTCAAAATTTTTAAAGATGATAGAGGATAAAGATATTAGAGATTACATATTAAAATTTATAACA
TTTAAAATTATAAATGACGAAAATCAATTAAATGAATTACTAAATGATAGTAGCTATACAAAAATATTGGCTATCGGAGG
AAAAGCCACTATAAAAACAGATGACAATTTAAAAGTAATAGGCAAAGACAACGAATATGCCTGTGGAGATTGTGTAATTG
GAAAGGGTGGAACTACTCCAATATCCCGTATGGAAGGTAAAGTAGTTGCCGAAAATATTTATAATGAATTAAACAATAAA
CCACCCATAAAACCAAATTACAATAATATCCCAAATACTATTAGATTGGATTTAAATATATCTTATGTTGGAAAACAGAC
AGATGATTTTAAAATTATTCCTAATAGTGTTGGAAAAGGGGATTTTTTTAGGGTTTTCAACCACATAGGCATAAATAAAA
TATATTACGAAAATAATAGGGTAGTGGGTGCAATTTCAATGTCTCCATCAATGGAAACAATTCCATATTTTGCCCAGTAT
TTAAATGGTGTCAATATTTATGATGGTTTTATAGAGGTTCATCCTTCAACAGACCCATTTTATAAAATATTTGGTAGTAG
GTAA

Upstream 100 bases:

>100_bases
GGTATATATTAAATAACATAATAAACTATATAATATTATTAAACTATAAAACATATCCATATATTGTATATATATTGTAT
ATGTCATTCTTGGTGTAATT

Downstream 100 bases:

>100_bases
TATATTATGGATTTCACGATTCTCCAATCATTTCTTATGAATCTCGTAGAAAAATATGGGTTATTGGGTATATTTATAAT
CGGGTTTTCAGAACCAATAT

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: protein N6-(lipoyl)lysine; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MELQNFSEKNFYSRKNSKAILRPPENTKNIENTKIAVIGGGPAGKYCSTELAKKGFSVSIYEKNKIGGTCLNYGCTYVTG
LREMADIINNLNIVNSNKEIKDNTKLEDVISFKELQKNISNILTKIRGKLEGDLLKYNNIKLYNEEFKEEYKKNYDYIVY
ATGKEYTNNYNGVECLIHSDIVNLEELPEKILIVGGGTVAMEYASLFSNYGCEVVVYVRSKFLKMIEDKDIRDYILKFIT
FKIINDENQLNELLNDSSYTKILAIGGKATIKTDDNLKVIGKDNEYACGDCVIGKGGTTPISRMEGKVVAENIYNELNNK
PPIKPNYNNIPNTIRLDLNISYVGKQTDDFKIIPNSVGKGDFFRVFNHIGINKIYYENNRVVGAISMSPSMETIPYFAQY
LNGVNIYDGFIEVHPSTDPFYKIFGSR

Sequences:

>Translated_427_residues
MELQNFSEKNFYSRKNSKAILRPPENTKNIENTKIAVIGGGPAGKYCSTELAKKGFSVSIYEKNKIGGTCLNYGCTYVTG
LREMADIINNLNIVNSNKEIKDNTKLEDVISFKELQKNISNILTKIRGKLEGDLLKYNNIKLYNEEFKEEYKKNYDYIVY
ATGKEYTNNYNGVECLIHSDIVNLEELPEKILIVGGGTVAMEYASLFSNYGCEVVVYVRSKFLKMIEDKDIRDYILKFIT
FKIINDENQLNELLNDSSYTKILAIGGKATIKTDDNLKVIGKDNEYACGDCVIGKGGTTPISRMEGKVVAENIYNELNNK
PPIKPNYNNIPNTIRLDLNISYVGKQTDDFKIIPNSVGKGDFFRVFNHIGINKIYYENNRVVGAISMSPSMETIPYFAQY
LNGVNIYDGFIEVHPSTDPFYKIFGSR
>Mature_427_residues
MELQNFSEKNFYSRKNSKAILRPPENTKNIENTKIAVIGGGPAGKYCSTELAKKGFSVSIYEKNKIGGTCLNYGCTYVTG
LREMADIINNLNIVNSNKEIKDNTKLEDVISFKELQKNISNILTKIRGKLEGDLLKYNNIKLYNEEFKEEYKKNYDYIVY
ATGKEYTNNYNGVECLIHSDIVNLEELPEKILIVGGGTVAMEYASLFSNYGCEVVVYVRSKFLKMIEDKDIRDYILKFIT
FKIINDENQLNELLNDSSYTKILAIGGKATIKTDDNLKVIGKDNEYACGDCVIGKGGTTPISRMEGKVVAENIYNELNNK
PPIKPNYNNIPNTIRLDLNISYVGKQTDDFKIIPNSVGKGDFFRVFNHIGINKIYYENNRVVGAISMSPSMETIPYFAQY
LNGVNIYDGFIEVHPSTDPFYKIFGSR

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI291045266, Length=342, Percent_Identity=23.0994152046784, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI291045268, Length=418, Percent_Identity=21.7703349282297, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.4

Molecular weight: Translated: 48365; Mature: 48365

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQNFSEKNFYSRKNSKAILRPPENTKNIENTKIAVIGGGPAGKYCSTELAKKGFSVSI
CCCCCCCCCCHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
YEKNKIGGTCLNYGCTYVTGLREMADIINNLNIVNSNKEIKDNTKLEDVISFKELQKNIS
EECCCCCCHHHHCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
NILTKIRGKLEGDLLKYNNIKLYNEEFKEEYKKNYDYIVYATGKEYTNNYNGVECLIHSD
HHHHHHHCCCCCCEEEECCEEEECHHHHHHHHCCCCEEEEECCCHHCCCCCCEEEEEECC
IVNLEELPEKILIVGGGTVAMEYASLFSNYGCEVVVYVRSKFLKMIEDKDIRDYILKFIT
CCCHHHCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHE
FKIINDENQLNELLNDSSYTKILAIGGKATIKTDDNLKVIGKDNEYACGDCVIGKGGTTP
EEEECCHHHHHHHHCCCCCEEEEEECCEEEEEECCCEEEEECCCCEECCCEEECCCCCCC
ISRMEGKVVAENIYNELNNKPPIKPNYNNIPNTIRLDLNISYVGKQTDDFKIIPNSVGKG
HHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEECCCCCCCC
DFFRVFNHIGINKIYYENNRVVGAISMSPSMETIPYFAQYLNGVNIYDGFIEVHPSTDPF
HHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHCCCEEEECEEEECCCCCCH
YKIFGSR
HHCCCCC
>Mature Secondary Structure
MELQNFSEKNFYSRKNSKAILRPPENTKNIENTKIAVIGGGPAGKYCSTELAKKGFSVSI
CCCCCCCCCCHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
YEKNKIGGTCLNYGCTYVTGLREMADIINNLNIVNSNKEIKDNTKLEDVISFKELQKNIS
EECCCCCCHHHHCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHH
NILTKIRGKLEGDLLKYNNIKLYNEEFKEEYKKNYDYIVYATGKEYTNNYNGVECLIHSD
HHHHHHHCCCCCCEEEECCEEEECHHHHHHHHCCCCEEEEECCCHHCCCCCCEEEEEECC
IVNLEELPEKILIVGGGTVAMEYASLFSNYGCEVVVYVRSKFLKMIEDKDIRDYILKFIT
CCCHHHCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHE
FKIINDENQLNELLNDSSYTKILAIGGKATIKTDDNLKVIGKDNEYACGDCVIGKGGTTP
EEEECCHHHHHHHHCCCCCEEEEEECCEEEEEECCCEEEEECCCCEECCCEEECCCCCCC
ISRMEGKVVAENIYNELNNKPPIKPNYNNIPNTIRLDLNISYVGKQTDDFKIIPNSVGKG
HHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEECCCCCCCC
DFFRVFNHIGINKIYYENNRVVGAISMSPSMETIPYFAQYLNGVNIYDGFIEVHPSTDPF
HHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHCCCEEEECEEEECCCCCCH
YKIFGSR
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADH [C]

Metal ions: NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {lipoamide}} 1.1 {NAD+}} [C]

Substrates: protein N6-(dihydrolipoyl)lysine; NAD+

Specific reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; p-Aminophenyl dichloroarsine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]