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The map label for this gene is sepS

Identifier: 150401484

GI number: 150401484

Start: 1095015

End: 1096637

Strand: Direct

Name: sepS

Synonym: Maeo_1060

Alternate gene names: 150401484

Gene position: 1095015-1096637 (Clockwise)

Preceding gene: 150401483

Following gene: 150401497

Centisome position: 69.77

GC content: 31.92

Gene sequence:

>1623_bases
ATGTTTGATACAAAAAAAGTTTTAGAATTGGCAAATAAAGATTTTGAAAAAGCTTGGATAACTACAAAGGATTTAATAAA
AGATGCCCCAATAAATAAAAAATACCCAAGAATAAAACCATCATTTGGAAAAACAAATCCAGTAATGGATACAATTGAAC
AGCTTAGGCAGGCATATTTAAGGATGGGATTTGAAGAATATATAAATCCTGTAATTGTAGATGAAAAGGACATATACAAA
CAATTTGGACCAGAAGCTATGGCTGTATTGGATAGATGTTTTTATTTAGCTGGATTGCCAAGACCAGACATAGGGCTAAG
TAATGATAAAATTGAACAGATTGAAAAATTAGGCATAAAAATAGACAGCAACGAAAAAAAGGAGAATTTAAGGAAAACAC
TTCATTTATACAAAAAGGGAGTGTTGGAAGGAGACGATTTAGTATATGAAATAGCAAATAGTTTAGGATTATCCAATGAA
ATGGGATTAAAAATATTGGAAGAAGTATTTCCCGAATTTAAAAACTTAAAGGCCGAATCATTACCTTTAACTCTTAGAAG
TCATATGACATCTGGCTGGTTTATAACAATATCTGAAATGATGGGAAAAAAACCACTCCCCTATGCCCTGTTTTCAATTG
ATAGATGTTTTAGAAGGGAGCAAAAAGAGGATAAAAGCCATTTAATGACATATCATTCTGCTTCCTGTGTCCTGGTGGGA
GAAGATATTACTCTTGATGATGGAAAAGCCATAGCCGAAGGGTTGTTATCACAATTTGGATTTACGGACTTCCAATTTAG
GCCAGATGAGAAAAAAAGTAAATACTACACCCCGGAAACTCAAACGGAGGTATATGCATACCACCCCAAATTAAAGGAAT
GGCTTGAAGTTGCCACATTTGGAATATATTCACCAATTGCTTTAAGTAAATACAATATTAGCGTTCCCGTTATGAATTTG
GGGCTTGGAGTGGAAAGACTTGCAATGATTAACCATAATTACGAGGATGTAAGAAAAATGGTTTATCCTCAATTTTATGA
ACAGACCCTTAGCGATAGAGATATAGCATATAGTATAAAAGTAGATAAAGTGCCCGTCCTTGATGAATTAAAGGATTTAA
CAGGGGAATTAATAGAATTATGTGTTAAAAATAAAGATAAACAGTCTCCTTGTGAGGTATTTATTGAAAAGAAAATAAAA
TTCTACAACACCACTAAAACCATAAAAATAACGCTATTTGAAAAAGAGGAAGGTAAAAATTTATTGGGTCCCTCAATATT
AAATAAAATATTTGTGCACAATGGAAATATATTTGGAGTTCCTGAATCGTTTGACAATGTTAAAGAGGAATTTGTGAAAG
TTTTAAGTGAGGCCAAAAATAAGGGAGCTCCCACAAATTTAACTTATATAGATACAATATGTTATAAAATAACATCAAAA
ATAGAGGAGGCCCTTATCTCAAATACTAAAAAGTTAAAAATCAGAGCTCCGATTGTAAGAAGTTTAAGCGATGTAAATTT
AAAAATAGATGAGTTGGCTTTAAAGCAGATAATGGGAAACAACAAGGTTATAGACATAAGAGGTCCCGTATTTTTAAATG
TGAAATGTGAAATAAATGATTAA

Upstream 100 bases:

>100_bases
ATTTAACGCTTGGTAAAGATATTCGTCTTAATAGAAATAAAAAATAAAAGTTTTATAAGATTAAATAAAATAAAAATACT
GAAAAAAAGGAATGATATAA

Downstream 100 bases:

>100_bases
ATCTTTTATTTATTTTTATTATTTTTTTATTTTATTATAAATACTATTAAATACTATTATTCTAGGGGATTAACATAATA
TACAATTTTATTTCCATCTT

Product: O-phosphoseryl-tRNA synthetase

Products: NA

Alternate protein names: SepRS; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MFDTKKVLELANKDFEKAWITTKDLIKDAPINKKYPRIKPSFGKTNPVMDTIEQLRQAYLRMGFEEYINPVIVDEKDIYK
QFGPEAMAVLDRCFYLAGLPRPDIGLSNDKIEQIEKLGIKIDSNEKKENLRKTLHLYKKGVLEGDDLVYEIANSLGLSNE
MGLKILEEVFPEFKNLKAESLPLTLRSHMTSGWFITISEMMGKKPLPYALFSIDRCFRREQKEDKSHLMTYHSASCVLVG
EDITLDDGKAIAEGLLSQFGFTDFQFRPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGIYSPIALSKYNISVPVMNL
GLGVERLAMINHNYEDVRKMVYPQFYEQTLSDRDIAYSIKVDKVPVLDELKDLTGELIELCVKNKDKQSPCEVFIEKKIK
FYNTTKTIKITLFEKEEGKNLLGPSILNKIFVHNGNIFGVPESFDNVKEEFVKVLSEAKNKGAPTNLTYIDTICYKITSK
IEEALISNTKKLKIRAPIVRSLSDVNLKIDELALKQIMGNNKVIDIRGPVFLNVKCEIND

Sequences:

>Translated_540_residues
MFDTKKVLELANKDFEKAWITTKDLIKDAPINKKYPRIKPSFGKTNPVMDTIEQLRQAYLRMGFEEYINPVIVDEKDIYK
QFGPEAMAVLDRCFYLAGLPRPDIGLSNDKIEQIEKLGIKIDSNEKKENLRKTLHLYKKGVLEGDDLVYEIANSLGLSNE
MGLKILEEVFPEFKNLKAESLPLTLRSHMTSGWFITISEMMGKKPLPYALFSIDRCFRREQKEDKSHLMTYHSASCVLVG
EDITLDDGKAIAEGLLSQFGFTDFQFRPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGIYSPIALSKYNISVPVMNL
GLGVERLAMINHNYEDVRKMVYPQFYEQTLSDRDIAYSIKVDKVPVLDELKDLTGELIELCVKNKDKQSPCEVFIEKKIK
FYNTTKTIKITLFEKEEGKNLLGPSILNKIFVHNGNIFGVPESFDNVKEEFVKVLSEAKNKGAPTNLTYIDTICYKITSK
IEEALISNTKKLKIRAPIVRSLSDVNLKIDELALKQIMGNNKVIDIRGPVFLNVKCEIND
>Mature_540_residues
MFDTKKVLELANKDFEKAWITTKDLIKDAPINKKYPRIKPSFGKTNPVMDTIEQLRQAYLRMGFEEYINPVIVDEKDIYK
QFGPEAMAVLDRCFYLAGLPRPDIGLSNDKIEQIEKLGIKIDSNEKKENLRKTLHLYKKGVLEGDDLVYEIANSLGLSNE
MGLKILEEVFPEFKNLKAESLPLTLRSHMTSGWFITISEMMGKKPLPYALFSIDRCFRREQKEDKSHLMTYHSASCVLVG
EDITLDDGKAIAEGLLSQFGFTDFQFRPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGIYSPIALSKYNISVPVMNL
GLGVERLAMINHNYEDVRKMVYPQFYEQTLSDRDIAYSIKVDKVPVLDELKDLTGELIELCVKNKDKQSPCEVFIEKKIK
FYNTTKTIKITLFEKEEGKNLLGPSILNKIFVHNGNIFGVPESFDNVKEEFVKVLSEAKNKGAPTNLTYIDTICYKITSK
IEEALISNTKKLKIRAPIVRSLSDVNLKIDELALKQIMGNNKVIDIRGPVFLNVKCEIND

Specific function: Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys)

COG id: COG2024

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit (archaeal type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=313, Percent_Identity=25.2396166134185, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32563657, Length=311, Percent_Identity=23.7942122186495, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17508957, Length=311, Percent_Identity=23.7942122186495, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321087, Length=171, Percent_Identity=32.1637426900585, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI18858079, Length=138, Percent_Identity=31.8840579710145, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SEPS_META3 (A6UVW6)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325250.1
- ProteinModelPortal:   A6UVW6
- SMR:   A6UVW6
- STRING:   A6UVW6
- GeneID:   5326944
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1060
- eggNOG:   arNOG04874
- HOGENOM:   HBG539012
- OMA:   TLRSHMT
- ProtClustDB:   PRK06253
- BioCyc:   MAEO419665:MAEO_1060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01674
- InterPro:   IPR006195
- InterPro:   IPR005246
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00470

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.27

Molecular weight: Translated: 61922; Mature: 61922

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDTKKVLELANKDFEKAWITTKDLIKDAPINKKYPRIKPSFGKTNPVMDTIEQLRQAYL
CCCHHHHHHHHCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RMGFEEYINPVIVDEKDIYKQFGPEAMAVLDRCFYLAGLPRPDIGLSNDKIEQIEKLGIK
HCCHHHHCCCEEECCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCE
IDSNEKKENLRKTLHLYKKGVLEGDDLVYEIANSLGLSNEMGLKILEEVFPEFKNLKAES
ECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
LPLTLRSHMTSGWFITISEMMGKKPLPYALFSIDRCFRREQKEDKSHLMTYHSASCVLVG
CCCHHHHHCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEE
EDITLDDGKAIAEGLLSQFGFTDFQFRPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATF
CCEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
GIYSPIALSKYNISVPVMNLGLGVERLAMINHNYEDVRKMVYPQFYEQTLSDRDIAYSIK
HCCCCCEEEEEECCCCCEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
VDKVPVLDELKDLTGELIELCVKNKDKQSPCEVFIEKKIKFYNTTKTIKITLFEKEEGKN
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCEEEEEEEEECCCCCC
LLGPSILNKIFVHNGNIFGVPESFDNVKEEFVKVLSEAKNKGAPTNLTYIDTICYKITSK
CCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IEEALISNTKKLKIRAPIVRSLSDVNLKIDELALKQIMGNNKVIDIRGPVFLNVKCEIND
HHHHHHCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEEEECC
>Mature Secondary Structure
MFDTKKVLELANKDFEKAWITTKDLIKDAPINKKYPRIKPSFGKTNPVMDTIEQLRQAYL
CCCHHHHHHHHCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RMGFEEYINPVIVDEKDIYKQFGPEAMAVLDRCFYLAGLPRPDIGLSNDKIEQIEKLGIK
HCCHHHHCCCEEECCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCE
IDSNEKKENLRKTLHLYKKGVLEGDDLVYEIANSLGLSNEMGLKILEEVFPEFKNLKAES
ECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
LPLTLRSHMTSGWFITISEMMGKKPLPYALFSIDRCFRREQKEDKSHLMTYHSASCVLVG
CCCHHHHHCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEE
EDITLDDGKAIAEGLLSQFGFTDFQFRPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATF
CCEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
GIYSPIALSKYNISVPVMNLGLGVERLAMINHNYEDVRKMVYPQFYEQTLSDRDIAYSIK
HCCCCCEEEEEECCCCCEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
VDKVPVLDELKDLTGELIELCVKNKDKQSPCEVFIEKKIKFYNTTKTIKITLFEKEEGKN
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCEEEEEEEEECCCCCC
LLGPSILNKIFVHNGNIFGVPESFDNVKEEFVKVLSEAKNKGAPTNLTYIDTICYKITSK
CCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IEEALISNTKKLKIRAPIVRSLSDVNLKIDELALKQIMGNNKVIDIRGPVFLNVKCEIND
HHHHHHCCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA