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Identifier: 150401292

GI number: 150401292

Start: 891613

End: 893301

Strand: Direct

Name: lig

Synonym: Maeo_0864

Alternate gene names: 150401292

Gene position: 891613-893301 (Clockwise)

Preceding gene: 150401291

Following gene: 150401294

Centisome position: 56.81

GC content: 31.62

Gene sequence:

>1689_bases
ATGGCAATATTGTTTAAAGAAGTATGTGATATATTTTATAAAATAGAAAGCACAACAAAAAGATTGGAAAAAATGGAGTA
TTTTATAGAATTAATTAAAATGGTGGAAAATAAAAAAGCTCCACAAGATTTAAAAAAAATATGTCAAATTGCAGTTGGGA
GAGTGTTTGCAGAGCATGAAAATAAAGAATTAGGAATTGGACCCAACATATTAATCGATGCCATAATATCCACGGGAATA
AATAAAAAAACGATACAAAACACCATAAACCAGACAGGAGATATAGGAACGGCCCTAGAACAGTTGGGGCATAATATAAA
ACAGACCTCTTTATTTCAAACACCCCCTACGCTGGAAGAAGTATATACTACATTGAAAAAATTATCGACTATCGAAGGCG
GACAATCTCAAAAAAAGAAAATAAGGCATATTTCAAGTATTCTTATAAAATCGTCCCCAATAGAACAAAGATATTTGGCA
AGATTAATTTTAGAGGATATGAGAATAGGAATGAGTATTCCAACAATATTGGGGGCTTTTTCAAAATATTACAACCAACC
CAAAGAAAATTTGGAAAAAATATATGCCGTAGTTAATGATATTGGGCTATTGGCGGAAAAATTGGCACAACGATGCGATA
TTTATAATGATGAAGAACTACAATTAAAAATATTTAGGCCAATTAAACCGATGTTAGCTCAACTCATTGGCTCCATAAAT
GATGCAATTATTGAAATGGGAGCTCCCCAATTTGAAACCAAATACGATGGTGCAAGAGTTCAAATTCATAAAAAAGATAA
TATAGTAAAAATATACAGCAGGAAATTAGAAGACATAACAAACTCCATACCCGAAATAGTAGAAGAAGTAAAAAATATTG
AAGCTCAAAACCTAATTATTGAAGGAGAATGTGTAGCAATGGATAAAAACGGGAGACCTAGACCCTTCCAAGACATACTG
CGGAGATTTAGAAGAAAATATAATATAGATTCCATACAAAGTGAAATAAATTTAAAGGTTTATGTATTTGATATTCTTTA
TTATAACAATAAATCATTGATAGAGTTACCATTAATCGAAAGAAGAAGCATATTAGAGAAAATACTAACCAACAAACATA
ATAAATTAAATATATCCCACAAACTAACCACCGACAACGAACAGAAAGCCAGAGAATTCTATGAATGGAGTTTAAGCATA
GGGCATGAAGGCGTAATGATTAAAAACCCAAATGCAATATATACTCCCGGAAGCAGAGTAAGAACTATGTATAAATTTAA
ACCAACTTTGGAAAGTTTAGATGTGGTAATTACAAAGGCCAAAATGGGAATGGGAAAAAGAAAAGAATGGTATGGTTCCT
TTGAAATTGCCGTAAAAGATTATGAAAATAATTTATACACCATTGGGCATGTTGGAAGTGGATTAACCGAGGAGGAGCTC
CACAACCTGACAGAGCAAATAAAAAATATAACAATTGAAGTAATTAATGACGAGGCCATTGTAGAGCCTAAAATAGTTTT
AGAAATATCCTATGAGGAAATTCAAGAATCGGATAAATACAAATGTGGTTATGCTCTTAGATTTCCACGAGTGGCAAGAA
TTAGGACAGATAAATCCATTGAGGACATAAATTCCATTGAGGATATTGAAAGAATATTTGATATTCAAAAAGGAAAAGGA
AATTTATAA

Upstream 100 bases:

>100_bases
TATTGAATCCCAGGGGGACCTCAAAATTCCAGAGGAATTTTTACAATAGGACGAATAAGGACGGTTATTGAACTTACTAT
ATTTTAAAAGCGGTGAGTAA

Downstream 100 bases:

>100_bases
TTAATCTTCAATAATTATATTAGATAAATTCATGAAAAAGACCGAAGATTGACTAAATAATTTTTGGGAGCTCCCATCAT
ATATTTTATTATTTCATTTG

Product: DNA ligase I, ATP-dependent Dnl1

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MAILFKEVCDIFYKIESTTKRLEKMEYFIELIKMVENKKAPQDLKKICQIAVGRVFAEHENKELGIGPNILIDAIISTGI
NKKTIQNTINQTGDIGTALEQLGHNIKQTSLFQTPPTLEEVYTTLKKLSTIEGGQSQKKKIRHISSILIKSSPIEQRYLA
RLILEDMRIGMSIPTILGAFSKYYNQPKENLEKIYAVVNDIGLLAEKLAQRCDIYNDEELQLKIFRPIKPMLAQLIGSIN
DAIIEMGAPQFETKYDGARVQIHKKDNIVKIYSRKLEDITNSIPEIVEEVKNIEAQNLIIEGECVAMDKNGRPRPFQDIL
RRFRRKYNIDSIQSEINLKVYVFDILYYNNKSLIELPLIERRSILEKILTNKHNKLNISHKLTTDNEQKAREFYEWSLSI
GHEGVMIKNPNAIYTPGSRVRTMYKFKPTLESLDVVITKAKMGMGKRKEWYGSFEIAVKDYENNLYTIGHVGSGLTEEEL
HNLTEQIKNITIEVINDEAIVEPKIVLEISYEEIQESDKYKCGYALRFPRVARIRTDKSIEDINSIEDIERIFDIQKGKG
NL

Sequences:

>Translated_562_residues
MAILFKEVCDIFYKIESTTKRLEKMEYFIELIKMVENKKAPQDLKKICQIAVGRVFAEHENKELGIGPNILIDAIISTGI
NKKTIQNTINQTGDIGTALEQLGHNIKQTSLFQTPPTLEEVYTTLKKLSTIEGGQSQKKKIRHISSILIKSSPIEQRYLA
RLILEDMRIGMSIPTILGAFSKYYNQPKENLEKIYAVVNDIGLLAEKLAQRCDIYNDEELQLKIFRPIKPMLAQLIGSIN
DAIIEMGAPQFETKYDGARVQIHKKDNIVKIYSRKLEDITNSIPEIVEEVKNIEAQNLIIEGECVAMDKNGRPRPFQDIL
RRFRRKYNIDSIQSEINLKVYVFDILYYNNKSLIELPLIERRSILEKILTNKHNKLNISHKLTTDNEQKAREFYEWSLSI
GHEGVMIKNPNAIYTPGSRVRTMYKFKPTLESLDVVITKAKMGMGKRKEWYGSFEIAVKDYENNLYTIGHVGSGLTEEEL
HNLTEQIKNITIEVINDEAIVEPKIVLEISYEEIQESDKYKCGYALRFPRVARIRTDKSIEDINSIEDIERIFDIQKGKG
NL
>Mature_561_residues
AILFKEVCDIFYKIESTTKRLEKMEYFIELIKMVENKKAPQDLKKICQIAVGRVFAEHENKELGIGPNILIDAIISTGIN
KKTIQNTINQTGDIGTALEQLGHNIKQTSLFQTPPTLEEVYTTLKKLSTIEGGQSQKKKIRHISSILIKSSPIEQRYLAR
LILEDMRIGMSIPTILGAFSKYYNQPKENLEKIYAVVNDIGLLAEKLAQRCDIYNDEELQLKIFRPIKPMLAQLIGSIND
AIIEMGAPQFETKYDGARVQIHKKDNIVKIYSRKLEDITNSIPEIVEEVKNIEAQNLIIEGECVAMDKNGRPRPFQDILR
RFRRKYNIDSIQSEINLKVYVFDILYYNNKSLIELPLIERRSILEKILTNKHNKLNISHKLTTDNEQKAREFYEWSLSIG
HEGVMIKNPNAIYTPGSRVRTMYKFKPTLESLDVVITKAKMGMGKRKEWYGSFEIAVKDYENNLYTIGHVGSGLTEEELH
NLTEQIKNITIEVINDEAIVEPKIVLEISYEEIQESDKYKCGYALRFPRVARIRTDKSIEDINSIEDIERIFDIQKGKGN
L

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=621, Percent_Identity=26.8921095008052, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI46255052, Length=614, Percent_Identity=25.5700325732899, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI23199993, Length=614, Percent_Identity=25.5700325732899, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI148539894, Length=614, Percent_Identity=25.5700325732899, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI73747829, Length=478, Percent_Identity=23.0125523012552, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI73747844, Length=478, Percent_Identity=23.0125523012552, Blast_Score=116, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71985269, Length=616, Percent_Identity=26.9480519480519, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI71985278, Length=190, Percent_Identity=28.4210526315789, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320038, Length=615, Percent_Identity=28.4552845528455, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6324578, Length=324, Percent_Identity=25.6172839506173, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762424, Length=606, Percent_Identity=26.7326732673267, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI85815841, Length=463, Percent_Identity=26.5658747300216, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24641829, Length=498, Percent_Identity=21.6867469879518, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI_META3 (A6UVC4)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325058.1
- ProteinModelPortal:   A6UVC4
- SMR:   A6UVC4
- STRING:   A6UVC4
- GeneID:   5326248
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0864
- eggNOG:   arNOG04596
- HOGENOM:   HBG570821
- OMA:   GRPRPFQ
- ProtClustDB:   CLSK876100
- BioCyc:   MAEO419665:MAEO_0864-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 64755; Mature: 64624

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 254-254 BINDING 252-252 BINDING 259-259 BINDING 274-274 BINDING 303-303 BINDING 343-343 BINDING 419-419 BINDING 425-425

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILFKEVCDIFYKIESTTKRLEKMEYFIELIKMVENKKAPQDLKKICQIAVGRVFAEHE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
NKELGIGPNILIDAIISTGINKKTIQNTINQTGDIGTALEQLGHNIKQTSLFQTPPTLEE
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCCCHHH
VYTTLKKLSTIEGGQSQKKKIRHISSILIKSSPIEQRYLARLILEDMRIGMSIPTILGAF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
SKYYNQPKENLEKIYAVVNDIGLLAEKLAQRCDIYNDEELQLKIFRPIKPMLAQLIGSIN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
DAIIEMGAPQFETKYDGARVQIHKKDNIVKIYSRKLEDITNSIPEIVEEVKNIEAQNLII
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEE
EGECVAMDKNGRPRPFQDILRRFRRKYNIDSIQSEINLKVYVFDILYYNNKSLIELPLIE
ECEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHCCEEEEEEEEEEECCCCEEECCCHH
RRSILEKILTNKHNKLNISHKLTTDNEQKAREFYEWSLSIGHEGVMIKNPNAIYTPGSRV
HHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCEEECCCHHH
RTMYKFKPTLESLDVVITKAKMGMGKRKEWYGSFEIAVKDYENNLYTIGHVGSGLTEEEL
EEEEEECCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCEEEEECCCCCCCHHHH
HNLTEQIKNITIEVINDEAIVEPKIVLEISYEEIQESDKYKCGYALRFPRVARIRTDKSI
HHHHHHHHCCEEEEECCCCEECCEEEEEEEHHHHHCCCCCCCCEEECCCCHHHHCCCCCH
EDINSIEDIERIFDIQKGKGNL
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AILFKEVCDIFYKIESTTKRLEKMEYFIELIKMVENKKAPQDLKKICQIAVGRVFAEHE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
NKELGIGPNILIDAIISTGINKKTIQNTINQTGDIGTALEQLGHNIKQTSLFQTPPTLEE
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCCCHHH
VYTTLKKLSTIEGGQSQKKKIRHISSILIKSSPIEQRYLARLILEDMRIGMSIPTILGAF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
SKYYNQPKENLEKIYAVVNDIGLLAEKLAQRCDIYNDEELQLKIFRPIKPMLAQLIGSIN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHH
DAIIEMGAPQFETKYDGARVQIHKKDNIVKIYSRKLEDITNSIPEIVEEVKNIEAQNLII
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEE
EGECVAMDKNGRPRPFQDILRRFRRKYNIDSIQSEINLKVYVFDILYYNNKSLIELPLIE
ECEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHCCEEEEEEEEEEECCCCEEECCCHH
RRSILEKILTNKHNKLNISHKLTTDNEQKAREFYEWSLSIGHEGVMIKNPNAIYTPGSRV
HHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCEEECCCHHH
RTMYKFKPTLESLDVVITKAKMGMGKRKEWYGSFEIAVKDYENNLYTIGHVGSGLTEEEL
EEEEEECCCHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCEEEEECCCCCCCHHHH
HNLTEQIKNITIEVINDEAIVEPKIVLEISYEEIQESDKYKCGYALRFPRVARIRTDKSI
HHHHHHHHCCEEEEECCCCEECCEEEEEEEHHHHHCCCCCCCCEEECCCCHHHHCCCCCH
EDINSIEDIERIFDIQKGKGNL
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA