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The map label for this gene is leuA2 [H]

Identifier: 150401277

GI number: 150401277

Start: 876723

End: 878267

Strand: Direct

Name: leuA2 [H]

Synonym: Maeo_0849

Alternate gene names: 150401277

Gene position: 876723-878267 (Clockwise)

Preceding gene: 150401276

Following gene: 150401278

Centisome position: 55.86

GC content: 33.59

Gene sequence:

>1545_bases
ATGGATACATATGGCTCAACCAACGATATAATTAAAAATTCAATTAAAGACTTAAATCTACCTGAAAAAATTAAAATATT
TGATACTACCCTAAGGGATGGAGAACAAACCCCAGGAGTATCTCTAACACCTGATGAAAAGGTAGAAATTGCCATTAATC
TAAATAATCTTGGAGTAGATATAATAGAGGCAGGATTTCCAATATCTTCCCCTGGGGAGCAGGAGGCAATTAAAAAAATA
ACTTCCTTAAATCTAAATGCTGAAATTTGCGGACTTGCAAGAGCTGTTAAAAAAGATATTGATATAGCAGTAGATTGTGG
CGTGGATAGCATACATACATTTATAGCAACATCCCCACTACATAGAAAATATAAATTAAAAATGGATAAGGAACAAATAA
TAAATAAAGCAGTTGAATCAATTGAATATATAAAAGAAAGAGGGCTAAAAGTAGAATTCTCAGCCGAGGACGCCACACGA
ACAGAATTAGATTATTTGATTGAAGTTTATAAAAATGCCGTAGATGCAGGAGCAGATAGAATAAATGTTCCAGATACCGT
TGGAGTAATGATTCCAAGGGCTATGACATATTTAATAAGCGAAATAAAAAAAGAAATAAATGTTCCAATTGCCGTTCATT
GCCATAATGATTTTGGGCTTTCTGTGGCTAACTCAGTAGGGGCAGTCGAAGGAGGGGCAGAGCAAATTCACTGCACAATA
AACGGATTAGGTGAAAGAGCTGGAAACGCATCTCTTGAAGAAGCCGTAATGTCTTTAAAAATGATTTATGGAATAGAAAG
TAATATTAAAACTGAAAAATTAACAGAAATATCTAATTTAGTATCAAATCTTACAGGAATAAAAAACCAACCAAATAAGG
CAATAGTTGGAGAAAATTCATTTGCTCATGAAAGTGGAATTCATGCCCATGGAGTTTTAGCTCATGCCCTAACCTACGAA
CCTATTGACCCAGAAATAGTAGGGCAAAAAAGAAAAATCATACTTGGAAAACATACTGGAACTCATGCAATATTATCCAA
GTTAAAGGAACTTAATTATATTGTGGGAGAAAATATTACGGAAGAACAATTTAATGAAATCTCCAAAAGAATAAAAGATG
CAGGAGATAAAGGAAAAATGATAACTGACTTAGATGTTGTTGCAATAGTGGATGATATTACAGATAGAACCGCAGAATCA
GAAAGAGCTGTTAATTTAGAACAAATAGCCGTAATGACTGGAAATAAAATAATACCTACTGCAAGTGTCTCTTTAATCAT
GAATAATGAGAGATATACAACTTCAAAAGTAGGAGTTGGACCTGTTGATGCAGCTTTAAAAGCCATTTGTTCCATCACTG
GCGAAAAAATAAAAATAAAAGAATATCATATAAATGCCATTACAGGGGGAACTGATGCATTGGCAGAAGTTATTGTTATA
TTAGAAGGGCAGGGCAGGGAAATTACTGCAAAGGCTGCAAATGAAGACATAGTTAGGGCATCAGTTGAGGCTATTATTAA
CGGAATAAATAAAATAATGAAATAA

Upstream 100 bases:

>100_bases
TTACAATTAATTATTAGCTGTTAATTATTAGCGAATTTATTTTTATTTGATTTATATATTATATTACTAATATTTACTAC
CCTCATAATCTGGTGAAATT

Downstream 100 bases:

>100_bases
ATTATATAATATTTGGGAGCTCCCAAAAATGGGAGCTCCTTTATATATTAAATATTAATAATAAAAATATTAAAAATGAA
AAAGGCGATAAAATGAAAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MDTYGSTNDIIKNSIKDLNLPEKIKIFDTTLRDGEQTPGVSLTPDEKVEIAINLNNLGVDIIEAGFPISSPGEQEAIKKI
TSLNLNAEICGLARAVKKDIDIAVDCGVDSIHTFIATSPLHRKYKLKMDKEQIINKAVESIEYIKERGLKVEFSAEDATR
TELDYLIEVYKNAVDAGADRINVPDTVGVMIPRAMTYLISEIKKEINVPIAVHCHNDFGLSVANSVGAVEGGAEQIHCTI
NGLGERAGNASLEEAVMSLKMIYGIESNIKTEKLTEISNLVSNLTGIKNQPNKAIVGENSFAHESGIHAHGVLAHALTYE
PIDPEIVGQKRKIILGKHTGTHAILSKLKELNYIVGENITEEQFNEISKRIKDAGDKGKMITDLDVVAIVDDITDRTAES
ERAVNLEQIAVMTGNKIIPTASVSLIMNNERYTTSKVGVGPVDAALKAICSITGEKIKIKEYHINAITGGTDALAEVIVI
LEGQGREITAKAANEDIVRASVEAIINGINKIMK

Sequences:

>Translated_514_residues
MDTYGSTNDIIKNSIKDLNLPEKIKIFDTTLRDGEQTPGVSLTPDEKVEIAINLNNLGVDIIEAGFPISSPGEQEAIKKI
TSLNLNAEICGLARAVKKDIDIAVDCGVDSIHTFIATSPLHRKYKLKMDKEQIINKAVESIEYIKERGLKVEFSAEDATR
TELDYLIEVYKNAVDAGADRINVPDTVGVMIPRAMTYLISEIKKEINVPIAVHCHNDFGLSVANSVGAVEGGAEQIHCTI
NGLGERAGNASLEEAVMSLKMIYGIESNIKTEKLTEISNLVSNLTGIKNQPNKAIVGENSFAHESGIHAHGVLAHALTYE
PIDPEIVGQKRKIILGKHTGTHAILSKLKELNYIVGENITEEQFNEISKRIKDAGDKGKMITDLDVVAIVDDITDRTAES
ERAVNLEQIAVMTGNKIIPTASVSLIMNNERYTTSKVGVGPVDAALKAICSITGEKIKIKEYHINAITGGTDALAEVIVI
LEGQGREITAKAANEDIVRASVEAIINGINKIMK
>Mature_514_residues
MDTYGSTNDIIKNSIKDLNLPEKIKIFDTTLRDGEQTPGVSLTPDEKVEIAINLNNLGVDIIEAGFPISSPGEQEAIKKI
TSLNLNAEICGLARAVKKDIDIAVDCGVDSIHTFIATSPLHRKYKLKMDKEQIINKAVESIEYIKERGLKVEFSAEDATR
TELDYLIEVYKNAVDAGADRINVPDTVGVMIPRAMTYLISEIKKEINVPIAVHCHNDFGLSVANSVGAVEGGAEQIHCTI
NGLGERAGNASLEEAVMSLKMIYGIESNIKTEKLTEISNLVSNLTGIKNQPNKAIVGENSFAHESGIHAHGVLAHALTYE
PIDPEIVGQKRKIILGKHTGTHAILSKLKELNYIVGENITEEQFNEISKRIKDAGDKGKMITDLDVVAIVDDITDRTAES
ERAVNLEQIAVMTGNKIIPTASVSLIMNNERYTTSKVGVGPVDAALKAICSITGEKIKIKEYHINAITGGTDALAEVIVI
LEGQGREITAKAANEDIVRASVEAIINGINKIMK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=288, Percent_Identity=25, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI109150422, Length=299, Percent_Identity=24.0802675585284, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI109150427, Length=299, Percent_Identity=24.0802675585284, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1786261, Length=507, Percent_Identity=36.4891518737673, Blast_Score=312, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6320071, Length=377, Percent_Identity=35.5437665782493, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=35.4054054054054, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324225, Length=544, Percent_Identity=22.2426470588235, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=22.4778761061947, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55736; Mature: 55736

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTYGSTNDIIKNSIKDLNLPEKIKIFDTTLRDGEQTPGVSLTPDEKVEIAINLNNLGVD
CCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEE
IIEAGFPISSPGEQEAIKKITSLNLNAEICGLARAVKKDIDIAVDCGVDSIHTFIATSPL
EEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCC
HRKYKLKMDKEQIINKAVESIEYIKERGLKVEFSAEDATRTELDYLIEVYKNAVDAGADR
CCCEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
INVPDTVGVMIPRAMTYLISEIKKEINVPIAVHCHNDFGLSVANSVGAVEGGAEQIHCTI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCCCCCCEEEEEE
NGLGERAGNASLEEAVMSLKMIYGIESNIKTEKLTEISNLVSNLTGIKNQPNKAIVGENS
ECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
FAHESGIHAHGVLAHALTYEPIDPEIVGQKRKIILGKHTGTHAILSKLKELNYIVGENIT
CCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
EEQFNEISKRIKDAGDKGKMITDLDVVAIVDDITDRTAESERAVNLEQIAVMTGNKIIPT
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCHHHCCCHHHEEEECCCEECCC
ASVSLIMNNERYTTSKVGVGPVDAALKAICSITGEKIKIKEYHINAITGGTDALAEVIVI
CEEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCCHHHHHEEEEE
LEGQGREITAKAANEDIVRASVEAIINGINKIMK
EECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTYGSTNDIIKNSIKDLNLPEKIKIFDTTLRDGEQTPGVSLTPDEKVEIAINLNNLGVD
CCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEE
IIEAGFPISSPGEQEAIKKITSLNLNAEICGLARAVKKDIDIAVDCGVDSIHTFIATSPL
EEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCC
HRKYKLKMDKEQIINKAVESIEYIKERGLKVEFSAEDATRTELDYLIEVYKNAVDAGADR
CCCEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
INVPDTVGVMIPRAMTYLISEIKKEINVPIAVHCHNDFGLSVANSVGAVEGGAEQIHCTI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCCCCCCCCCCCEEEEEE
NGLGERAGNASLEEAVMSLKMIYGIESNIKTEKLTEISNLVSNLTGIKNQPNKAIVGENS
ECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
FAHESGIHAHGVLAHALTYEPIDPEIVGQKRKIILGKHTGTHAILSKLKELNYIVGENIT
CCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
EEQFNEISKRIKDAGDKGKMITDLDVVAIVDDITDRTAESERAVNLEQIAVMTGNKIIPT
HHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHCCCHHHCCCHHHEEEECCCEECCC
ASVSLIMNNERYTTSKVGVGPVDAALKAICSITGEKIKIKEYHINAITGGTDALAEVIVI
CEEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCCHHHHHEEEEE
LEGQGREITAKAANEDIVRASVEAIINGINKIMK
EECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]