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The map label for this gene is tuf

Identifier: 150401211

GI number: 150401211

Start: 805098

End: 806384

Strand: Reverse

Name: tuf

Synonym: Maeo_0782

Alternate gene names: 150401211

Gene position: 806384-805098 (Counterclockwise)

Preceding gene: 150401212

Following gene: 150401210

Centisome position: 51.38

GC content: 37.45

Gene sequence:

>1287_bases
ATGGCAAAAGAAAAACCCGTACTAAATGTTGCATTTATCGGACACGTAGATGCTGGTAAATCCACAACAGTAGGAAGATT
ATTATTGGACAGTGGAACAATTGACCCGCAAATAATAGAAAGATTAAAAAGAGAAGCTTCTGAAAAAGGTAAAGCAGGAT
TCGAATTTGCATATGTTATGGATGGATTAAAAGAAGAAAGAGAAAGAGGAGTTACAATCGATATTGCTCACAAGAAATTC
GAGAGTAAAAAATACGATGTAACAATTGTGGACTGCCCAGGGCACAGAGACTTCATTAAAAACATGATTACAGGAGCTTC
ACAAGCTGATGCAGCTGTATTGGTATGTGATGTAAATGATGCTAAAACAGGTCTTCAACCCCAAACAAGAGAGCACATCT
TCTTAATCAGAACATTAGGTGTAAACCAGTTAGCTGTTGCAATTAACAAAATGGATACCGTAGATTACAGTGAAGAAGAA
TACAAAGCTATGGTAGATATGCTTTCAAACCAATTATTAAAAATGTTAGGATACAACCCAGCTAACATACACTTTGTTCC
AGTAGCATCATTCGTTGGAGACAACAATGTTAAAAAATCTGATAAAATGCCATGGTACAAAGGTCCAACGCTAGTTGAAG
TAATCGATACATTCAACCCACCTGAAAAACCAACAAACTTACCATTAAGAATGCCTATTCAAGATGTATATACAATTACA
GGAGTAGGAACAGTTCCAGTAGGAAGAGTTGAAACAGGAGTTATGAAACCAGGGGACAAAGTTGTATTTGAACCAGCTGG
AGTAAGTGGAGAAATCAAAACAATTGAAGCTCACCACGAACAATTACCAAAAGCAGAACCTGGGGACAATGTTGGATTCA
ATGTAAGAGGAGTAGGTAAAAAAGACATTAAAAAAGGAGATGTTTTAGGACACCCAGACAACATCCCAACAGTAGCAGAA
GAATTCACAGCTCAAATCGTTGTATTACAGCACCCATCAGTTATGACCGTAGGATACACACCAGTATTCCACGCTCACAC
AGCACAGGTAGCATGTACATTCATGGAGCTCCAAAAGAAATTGAACCCAGCTACAGGAGAAGTTAAAGAAGAAAACCCAG
ACTTCTTAAAAGCAGGAGATGCAGCAATCGTTAAAATCATGCCAACAAAACCAATGGTTATTGAAAATGTTAAAGAAATT
CCACAACTCGGTAGATTCGCTATTAGAGATATGGGAATGACAGTAGCAGCTGGTATGTGTATTGATGTAAAAGCTAAAAA
CAAATAA

Upstream 100 bases:

>100_bases
TACTCCTTTGGCATAATGGATATTACAATTTATAGTTGTTGATATTATAAAATATTATATATGTTAAAAATATATATTTT
AAATAAAACTTGGTGATAAA

Downstream 100 bases:

>100_bases
TTTAATAATTGATTCAAATAATTATTAATAATATCTATATTTTTTTAACCTTTTTTAGGGTTTTAATTTTTTAGGGCGTA
AATCAGTTTTTTATATTAAA

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAKEKPVLNVAFIGHVDAGKSTTVGRLLLDSGTIDPQIIERLKREASEKGKAGFEFAYVMDGLKEERERGVTIDIAHKKF
ESKKYDVTIVDCPGHRDFIKNMITGASQADAAVLVCDVNDAKTGLQPQTREHIFLIRTLGVNQLAVAINKMDTVDYSEEE
YKAMVDMLSNQLLKMLGYNPANIHFVPVASFVGDNNVKKSDKMPWYKGPTLVEVIDTFNPPEKPTNLPLRMPIQDVYTIT
GVGTVPVGRVETGVMKPGDKVVFEPAGVSGEIKTIEAHHEQLPKAEPGDNVGFNVRGVGKKDIKKGDVLGHPDNIPTVAE
EFTAQIVVLQHPSVMTVGYTPVFHAHTAQVACTFMELQKKLNPATGEVKEENPDFLKAGDAAIVKIMPTKPMVIENVKEI
PQLGRFAIRDMGMTVAAGMCIDVKAKNK

Sequences:

>Translated_428_residues
MAKEKPVLNVAFIGHVDAGKSTTVGRLLLDSGTIDPQIIERLKREASEKGKAGFEFAYVMDGLKEERERGVTIDIAHKKF
ESKKYDVTIVDCPGHRDFIKNMITGASQADAAVLVCDVNDAKTGLQPQTREHIFLIRTLGVNQLAVAINKMDTVDYSEEE
YKAMVDMLSNQLLKMLGYNPANIHFVPVASFVGDNNVKKSDKMPWYKGPTLVEVIDTFNPPEKPTNLPLRMPIQDVYTIT
GVGTVPVGRVETGVMKPGDKVVFEPAGVSGEIKTIEAHHEQLPKAEPGDNVGFNVRGVGKKDIKKGDVLGHPDNIPTVAE
EFTAQIVVLQHPSVMTVGYTPVFHAHTAQVACTFMELQKKLNPATGEVKEENPDFLKAGDAAIVKIMPTKPMVIENVKEI
PQLGRFAIRDMGMTVAAGMCIDVKAKNK
>Mature_427_residues
AKEKPVLNVAFIGHVDAGKSTTVGRLLLDSGTIDPQIIERLKREASEKGKAGFEFAYVMDGLKEERERGVTIDIAHKKFE
SKKYDVTIVDCPGHRDFIKNMITGASQADAAVLVCDVNDAKTGLQPQTREHIFLIRTLGVNQLAVAINKMDTVDYSEEEY
KAMVDMLSNQLLKMLGYNPANIHFVPVASFVGDNNVKKSDKMPWYKGPTLVEVIDTFNPPEKPTNLPLRMPIQDVYTITG
VGTVPVGRVETGVMKPGDKVVFEPAGVSGEIKTIEAHHEQLPKAEPGDNVGFNVRGVGKKDIKKGDVLGHPDNIPTVAEE
FTAQIVVLQHPSVMTVGYTPVFHAHTAQVACTFMELQKKLNPATGEVKEENPDFLKAGDAAIVKIMPTKPMVIENVKEIP
QLGRFAIRDMGMTVAAGMCIDVKAKNK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=50.2242152466368, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=51.1210762331839, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=427, Percent_Identity=37.2365339578454, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI5729864, Length=427, Percent_Identity=37.2365339578454, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI194018522, Length=424, Percent_Identity=36.3207547169811, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI194018520, Length=424, Percent_Identity=36.3207547169811, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI194097354, Length=424, Percent_Identity=36.3207547169811, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI46094014, Length=424, Percent_Identity=35.6132075471698, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI34147630, Length=434, Percent_Identity=29.9539170506912, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI54607086, Length=374, Percent_Identity=25.1336898395722, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI45593140, Length=433, Percent_Identity=22.6327944572748, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI82546879, Length=407, Percent_Identity=24.8157248157248, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI157426893, Length=260, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4503507, Length=361, Percent_Identity=24.9307479224377, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789108, Length=430, Percent_Identity=32.3255813953488, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790412, Length=432, Percent_Identity=32.1759259259259, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789737, Length=432, Percent_Identity=32.1759259259259, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI2367247, Length=335, Percent_Identity=25.6716417910448, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI48994988, Length=264, Percent_Identity=29.5454545454545, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17552884, Length=438, Percent_Identity=50.9132420091324, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17569207, Length=438, Percent_Identity=50.9132420091324, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=48.1566820276498, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI115532067, Length=424, Percent_Identity=37.5, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI115532065, Length=424, Percent_Identity=37.5, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=35.6807511737089, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=58.1081081081081, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17556456, Length=439, Percent_Identity=32.1184510250569, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI25141371, Length=325, Percent_Identity=33.2307692307692, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17565438, Length=380, Percent_Identity=25.5263157894737, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=51.5625, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17506081, Length=291, Percent_Identity=22.3367697594502, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=255, Percent_Identity=26.2745098039216, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=436, Percent_Identity=51.8348623853211, Blast_Score=454, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6319594, Length=436, Percent_Identity=51.8348623853211, Blast_Score=454, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320377, Length=426, Percent_Identity=37.5586854460094, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322937, Length=467, Percent_Identity=29.7644539614561, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324761, Length=326, Percent_Identity=34.0490797546012, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323320, Length=314, Percent_Identity=26.7515923566879, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324650, Length=240, Percent_Identity=24.1666666666667, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=51.1210762331839, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=51.1210762331839, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=50.1123595505618, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=50.1123595505618, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=50.1123595505618, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=50.1123595505618, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45550900, Length=430, Percent_Identity=35.1162790697674, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI17137380, Length=426, Percent_Identity=34.5070422535211, Blast_Score=257, Evalue=9e-69,
Organism=Drosophila melanogaster, GI281363316, Length=424, Percent_Identity=32.0754716981132, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI17864358, Length=424, Percent_Identity=32.0754716981132, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI19921738, Length=424, Percent_Identity=28.3018867924528, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19922690, Length=327, Percent_Identity=25.3822629969419, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647048, Length=367, Percent_Identity=22.8882833787466, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24647046, Length=367, Percent_Identity=22.8882833787466, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EF1A_META3 (A6UV43)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324977.1
- ProteinModelPortal:   A6UV43
- SMR:   A6UV43
- STRING:   A6UV43
- GeneID:   5326906
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0782
- eggNOG:   arNOG04484
- HOGENOM:   HBG307581
- OMA:   AFIGHVD
- ProtClustDB:   PRK12317
- BioCyc:   MAEO419665:MAEO_0782-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_A
- InterPro:   IPR000795
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00483

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 46870; Mature: 46739

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKPVLNVAFIGHVDAGKSTTVGRLLLDSGTIDPQIIERLKREASEKGKAGFEFAYVM
CCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
DGLKEERERGVTIDIAHKKFESKKYDVTIVDCPGHRDFIKNMITGASQADAAVLVCDVND
HHHHHHHHCCCEEEEECHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCC
AKTGLQPQTREHIFLIRTLGVNQLAVAINKMDTVDYSEEEYKAMVDMLSNQLLKMLGYNP
CCCCCCCCCCCEEEEEEECCCCEEEHEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ANIHFVPVASFVGDNNVKKSDKMPWYKGPTLVEVIDTFNPPEKPTNLPLRMPIQDVYTIT
CCEEEEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEECCHHHEEEEE
GVGTVPVGRVETGVMKPGDKVVFEPAGVSGEIKTIEAHHEQLPKAEPGDNVGFNVRGVGK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCEEEECCCH
KDIKKGDVLGHPDNIPTVAEEFTAQIVVLQHPSVMTVGYTPVFHAHTAQVACTFMELQKK
HHCCCCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHH
LNPATGEVKEENPDFLKAGDAAIVKIMPTKPMVIENVKEIPQLGRFAIRDMGMTVAAGMC
CCCCCCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
IDVKAKNK
EEEEECCC
>Mature Secondary Structure 
AKEKPVLNVAFIGHVDAGKSTTVGRLLLDSGTIDPQIIERLKREASEKGKAGFEFAYVM
CCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
DGLKEERERGVTIDIAHKKFESKKYDVTIVDCPGHRDFIKNMITGASQADAAVLVCDVND
HHHHHHHHCCCEEEEECHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCC
AKTGLQPQTREHIFLIRTLGVNQLAVAINKMDTVDYSEEEYKAMVDMLSNQLLKMLGYNP
CCCCCCCCCCCEEEEEEECCCCEEEHEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
ANIHFVPVASFVGDNNVKKSDKMPWYKGPTLVEVIDTFNPPEKPTNLPLRMPIQDVYTIT
CCEEEEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEECCHHHEEEEE
GVGTVPVGRVETGVMKPGDKVVFEPAGVSGEIKTIEAHHEQLPKAEPGDNVGFNVRGVGK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCEEEECCCH
KDIKKGDVLGHPDNIPTVAEEFTAQIVVLQHPSVMTVGYTPVFHAHTAQVACTFMELQKK
HHCCCCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHH
LNPATGEVKEENPDFLKAGDAAIVKIMPTKPMVIENVKEIPQLGRFAIRDMGMTVAAGMC
CCCCCCCCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEE
IDVKAKNK
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA