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The map label for this gene is cofH

Identifier: 150401179

GI number: 150401179

Start: 772160

End: 773248

Strand: Direct

Name: cofH

Synonym: Maeo_0750

Alternate gene names: 150401179

Gene position: 772160-773248 (Clockwise)

Preceding gene: 150401177

Following gene: 150401180

Centisome position: 49.2

GC content: 31.59

Gene sequence:

>1089_bases
ATGGATTTTGATAGTTATGCGGATAATTACAGCAAAAGTATCTCAAAAAAACAATGTTTAGAATTATTTGAAGATAATGA
AAATCTTTTTAATATTTTGAATTGTGCAAATAAAATCAATAAAAAAATAAACGGAAACACCATAACATATGTTGTAAATA
GAAATATTAATTTTACAAATATTTGTGTGGGAGATTGTAAATTTTGTGCCTTTAAGGTTGATGAAAACCATAACGATGCC
TATTTTATTAATCCAAAAGAAATTGGAAAAAAAGCTTTTGAGGCAAAAAAATTGGGATGCACAGAGGTATGTATTCAGGG
CGGACTACGCAATAATATAGATGTGGAGCTCCAATCCAACATATTAAAAGAAGTTTGTTCTGCCACAAAACCACTTGGAG
GAATACATATTCATGCGTTTTCGCCCATGGAAGTAAAATTTGCATCCGAAAATTCTGGATTAGACATTAAAGAAGCACTA
ATGATATTAAAAGAAAACGGATTAAACACAATGCCGGGAACTGCTGCGGAAATATTGGATGACAATATTAGGGCCGAATT
ATGTCCTTCGAAATTATCTACCAAGGAATGGATAAAAATAGTAAAAACAGCACATAAAGTAGGAATTAAAACAACTTCTA
CTATGATGTATGGACATATTGAGGAATATAAACACATTGTAAATCATTTATTTATTTTAAAAGAAATTCAGGAAGAAACT
GGCGGAATTACGGAATTTGTACCACTTAGTTTTATGCATAAAATGGCACCAATATATTATGAAGGAAATGCAAGAGGAGG
GAGCTCCGGGATTGAAGATTTAAAAGTTTATGCCATTAGTAGAATGTTATTTGGAGATACTATTAAAAATATTCAAGTTT
CTTGGGTTAAATTGGGAATTAAATTGGCACAAATGGGATTAAAATGCGGTGCAAATGACTTTGGGGGGACACTTATGGAA
GAAAATATTTCAAAATCAGCCGGTGCAAGTTATGGAACTTATATGGGTGCCGAAGAAATTAGAAATGTTATAAATGCCAT
TGGTGGAGCTCCCAGAGAAAGAGATACATTTTATAATATTTTAGAGTAA

Upstream 100 bases:

>100_bases
ATAAGGGACAGATATATTGAAGATTAACTATATTATTGTAAGGGAGCTCCCAGGCATACTATTATATATACACATACAAT
TATTATCATTTGGTGATTTA

Downstream 100 bases:

>100_bases
ATAAAATAAAAACAGGTGAAAAATTGGAAATAAAAAGAAGATACTGCCTAAATAAAAAAGAAATAAAAAAATTAAAAGAA
GAACTAAATAATATATTTGA

Product: FO synthase subunit 2

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MDFDSYADNYSKSISKKQCLELFEDNENLFNILNCANKINKKINGNTITYVVNRNINFTNICVGDCKFCAFKVDENHNDA
YFINPKEIGKKAFEAKKLGCTEVCIQGGLRNNIDVELQSNILKEVCSATKPLGGIHIHAFSPMEVKFASENSGLDIKEAL
MILKENGLNTMPGTAAEILDDNIRAELCPSKLSTKEWIKIVKTAHKVGIKTTSTMMYGHIEEYKHIVNHLFILKEIQEET
GGITEFVPLSFMHKMAPIYYEGNARGGSSGIEDLKVYAISRMLFGDTIKNIQVSWVKLGIKLAQMGLKCGANDFGGTLME
ENISKSAGASYGTYMGAEEIRNVINAIGGAPRERDTFYNILE

Sequences:

>Translated_362_residues
MDFDSYADNYSKSISKKQCLELFEDNENLFNILNCANKINKKINGNTITYVVNRNINFTNICVGDCKFCAFKVDENHNDA
YFINPKEIGKKAFEAKKLGCTEVCIQGGLRNNIDVELQSNILKEVCSATKPLGGIHIHAFSPMEVKFASENSGLDIKEAL
MILKENGLNTMPGTAAEILDDNIRAELCPSKLSTKEWIKIVKTAHKVGIKTTSTMMYGHIEEYKHIVNHLFILKEIQEET
GGITEFVPLSFMHKMAPIYYEGNARGGSSGIEDLKVYAISRMLFGDTIKNIQVSWVKLGIKLAQMGLKCGANDFGGTLME
ENISKSAGASYGTYMGAEEIRNVINAIGGAPRERDTFYNILE
>Mature_362_residues
MDFDSYADNYSKSISKKQCLELFEDNENLFNILNCANKINKKINGNTITYVVNRNINFTNICVGDCKFCAFKVDENHNDA
YFINPKEIGKKAFEAKKLGCTEVCIQGGLRNNIDVELQSNILKEVCSATKPLGGIHIHAFSPMEVKFASENSGLDIKEAL
MILKENGLNTMPGTAAEILDDNIRAELCPSKLSTKEWIKIVKTAHKVGIKTTSTMMYGHIEEYKHIVNHLFILKEIQEET
GGITEFVPLSFMHKMAPIYYEGNARGGSSGIEDLKVYAISRMLFGDTIKNIQVSWVKLGIKLAQMGLKCGANDFGGTLME
ENISKSAGASYGTYMGAEEIRNVINAIGGAPRERDTFYNILE

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO)

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFH_META3 (A6UV11)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324945.1
- STRING:   A6UV11
- GeneID:   5326302
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0750
- eggNOG:   arNOG05042
- HOGENOM:   HBG312134
- OMA:   TMMYGHI
- ProtClustDB:   PRK07360
- BioCyc:   MAEO419665:MAEO_0750-MONOMER
- HAMAP:   MF_01612
- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004762
- SMART:   SM00729
- TIGRFAMs:   TIGR00423
- TIGRFAMs:   TIGR03551

Pfam domain/function: PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 40263; Mature: 40263

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDSYADNYSKSISKKQCLELFEDNENLFNILNCANKINKKINGNTITYVVNRNINFTN
CCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEE
ICVGDCKFCAFKVDENHNDAYFINPKEIGKKAFEAKKLGCTEVCIQGGLRNNIDVELQSN
EEECCCEEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEHHHH
ILKEVCSATKPLGGIHIHAFSPMEVKFASENSGLDIKEALMILKENGLNTMPGTAAEILD
HHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHC
DNIRAELCPSKLSTKEWIKIVKTAHKVGIKTTSTMMYGHIEEYKHIVNHLFILKEIQEET
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGITEFVPLSFMHKMAPIYYEGNARGGSSGIEDLKVYAISRMLFGDTIKNIQVSWVKLGI
CCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEHHHHHHHH
KLAQMGLKCGANDFGGTLMEENISKSAGASYGTYMGAEEIRNVINAIGGAPRERDTFYNI
HHHHHHHHCCCCCCCCHHHHHHCCHHCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
LE
CC
>Mature Secondary Structure
MDFDSYADNYSKSISKKQCLELFEDNENLFNILNCANKINKKINGNTITYVVNRNINFTN
CCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEE
ICVGDCKFCAFKVDENHNDAYFINPKEIGKKAFEAKKLGCTEVCIQGGLRNNIDVELQSN
EEECCCEEEEEEECCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEHHHH
ILKEVCSATKPLGGIHIHAFSPMEVKFASENSGLDIKEALMILKENGLNTMPGTAAEILD
HHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHC
DNIRAELCPSKLSTKEWIKIVKTAHKVGIKTTSTMMYGHIEEYKHIVNHLFILKEIQEET
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGITEFVPLSFMHKMAPIYYEGNARGGSSGIEDLKVYAISRMLFGDTIKNIQVSWVKLGI
CCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEHHHHHHHH
KLAQMGLKCGANDFGGTLMEENISKSAGASYGTYMGAEEIRNVINAIGGAPRERDTFYNI
HHHHHHHHCCCCCCCCHHHHHHCCHHCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
LE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA