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The map label for this gene is frvX [C]

Identifier: 150401138

GI number: 150401138

Start: 726186

End: 727241

Strand: Reverse

Name: frvX [C]

Synonym: Maeo_0709

Alternate gene names: 150401138

Gene position: 727241-726186 (Counterclockwise)

Preceding gene: 150401139

Following gene: 150401137

Centisome position: 46.34

GC content: 33.43

Gene sequence:

>1056_bases
ATGACTGTGGTTAATTATTTAAAAGAATTATCGACGATGGAAGGAATATCCGGAAGAGAAGATAGAATAAGAGATTATAT
GGAAGAAGAACTTAAAAAATATTGCGATGAAGTATTCGTTGATAAATTTGGAAATCTCATAGCCAAAAAAGGAAATACTG
GAAAAAAAATAATGATTGCAGCACATATGGACGAGATTGGTTTAATGGTTAAATATATTGATGATAAAGGATTTTTAAAA
TTCGTAAAAATTGGGGGAATTAATGACCAGATGCTTTTAAATCAAAAAGTAGTTGTTCATACGGATAAGGGAGATATAAT
TGGTGTTTTAGGTTCAAAACCGCCCCACAAAATGAAAGAGGAAGAAAGAAATAAATTAATGAAATCAGAATTTATGTTTA
TAGACATTGGGGCAAAAAATAAAGAAGATGCCATAAATATGGGGGTTGAAATAGGCTCATGGATATCATTTAAAACTGAA
TTTGATGTTCTTGGGAACAATAGAATTACAGGAAAAGCCTTTGACGATAGGGCAGGATGTGCTGTTTTGTTGGATGTGGC
AAAAAAATTAAAAGATATGGAATTAAATTGTCAATTATATTTGGTTGGAACAGTTCAGGAGGAAGTTGGGTTAAAAGGTG
CTAAAACTTCGGCATTTGGTATAAGCCCAGATGTTGCATTTGCACTTGATGTAACAATATGTGGGGACCACCCAGGTATA
ACAATGGAGGATGCTGCTGTTGAAATGGGAAAAGGTCCAGTATTTGGAATAGTTGATGCTGCTGGAAGAGGTTTAATAGC
TCACCCAAAAGTTATAACGATGGTAAAAGAAGTAAGTAAAAAATACGACATTCCTGTTCAATATGAAGTTGGAGACGGTG
GAACTACCGATGCCACTTCAATACAATTAACTCAGGATGGAGTGCCTACTGGAGTTATTTCTGTTCCTTCTAGATATATT
CATACCCCCGTTGAGATGATTGATATTGAGGATTTGGAAAAAACTTCAGATTTAATGTTAAAATGTGTAGTTGATTTAAA
TAATTATTTTGAATAA

Upstream 100 bases:

>100_bases
ATAATAAATAAAATTATATAATATAATTTGATAATGGTATATAATAAGTTAATTTAATTAATTTTAATTTTTATAATCAA
AAATAAAATATGGTGAAAAT

Downstream 100 bases:

>100_bases
TAAAATAAATATAATAAACATATTTAATCTTCGGACAATTTCACCGTAGTTAATCTTCGGACTATCTAGGACAAACAATG
GACAGTTATTGAAGATTAAC

Product: cellulase

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTVVNYLKELSTMEGISGREDRIRDYMEEELKKYCDEVFVDKFGNLIAKKGNTGKKIMIAAHMDEIGLMVKYIDDKGFLK
FVKIGGINDQMLLNQKVVVHTDKGDIIGVLGSKPPHKMKEEERNKLMKSEFMFIDIGAKNKEDAINMGVEIGSWISFKTE
FDVLGNNRITGKAFDDRAGCAVLLDVAKKLKDMELNCQLYLVGTVQEEVGLKGAKTSAFGISPDVAFALDVTICGDHPGI
TMEDAAVEMGKGPVFGIVDAAGRGLIAHPKVITMVKEVSKKYDIPVQYEVGDGGTTDATSIQLTQDGVPTGVISVPSRYI
HTPVEMIDIEDLEKTSDLMLKCVVDLNNYFE

Sequences:

>Translated_351_residues
MTVVNYLKELSTMEGISGREDRIRDYMEEELKKYCDEVFVDKFGNLIAKKGNTGKKIMIAAHMDEIGLMVKYIDDKGFLK
FVKIGGINDQMLLNQKVVVHTDKGDIIGVLGSKPPHKMKEEERNKLMKSEFMFIDIGAKNKEDAINMGVEIGSWISFKTE
FDVLGNNRITGKAFDDRAGCAVLLDVAKKLKDMELNCQLYLVGTVQEEVGLKGAKTSAFGISPDVAFALDVTICGDHPGI
TMEDAAVEMGKGPVFGIVDAAGRGLIAHPKVITMVKEVSKKYDIPVQYEVGDGGTTDATSIQLTQDGVPTGVISVPSRYI
HTPVEMIDIEDLEKTSDLMLKCVVDLNNYFE
>Mature_350_residues
TVVNYLKELSTMEGISGREDRIRDYMEEELKKYCDEVFVDKFGNLIAKKGNTGKKIMIAAHMDEIGLMVKYIDDKGFLKF
VKIGGINDQMLLNQKVVVHTDKGDIIGVLGSKPPHKMKEEERNKLMKSEFMFIDIGAKNKEDAINMGVEIGSWISFKTEF
DVLGNNRITGKAFDDRAGCAVLLDVAKKLKDMELNCQLYLVGTVQEEVGLKGAKTSAFGISPDVAFALDVTICGDHPGIT
MEDAAVEMGKGPVFGIVDAAGRGLIAHPKVITMVKEVSKKYDIPVQYEVGDGGTTDATSIQLTQDGVPTGVISVPSRYIH
TPVEMIDIEDLEKTSDLMLKCVVDLNNYFE

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=337, Percent_Identity=36.4985163204748, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082417, Length=346, Percent_Identity=35.5491329479769, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1788727, Length=338, Percent_Identity=32.8402366863905, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 38676; Mature: 38545

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVNYLKELSTMEGISGREDRIRDYMEEELKKYCDEVFVDKFGNLIAKKGNTGKKIMIA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEE
AHMDEIGLMVKYIDDKGFLKFVKIGGINDQMLLNQKVVVHTDKGDIIGVLGSKPPHKMKE
EECCCCCEEEEEECCCCCEEEEEECCCCCCEEECCEEEEECCCCCEEEECCCCCCHHHHH
EERNKLMKSEFMFIDIGAKNKEDAINMGVEIGSWISFKTEFDVLGNNRITGKAFDDRAGC
HHHHHHHHHCEEEEEECCCCCCCHHHCCEEECCEEEEEEEEEEECCCEEECCCCCCCCCH
AVLLDVAKKLKDMELNCQLYLVGTVQEEVGLKGAKTSAFGISPDVAFALDVTICGDHPGI
HHHHHHHHHHHCCCCCEEEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCC
TMEDAAVEMGKGPVFGIVDAAGRGLIAHPKVITMVKEVSKKYDIPVQYEVGDGGTTDATS
EECHHHHHCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
IQLTQDGVPTGVISVPSRYIHTPVEMIDIEDLEKTSDLMLKCVVDLNNYFE
EEEECCCCCCCEEECCHHHHCCCHHEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVVNYLKELSTMEGISGREDRIRDYMEEELKKYCDEVFVDKFGNLIAKKGNTGKKIMIA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEE
AHMDEIGLMVKYIDDKGFLKFVKIGGINDQMLLNQKVVVHTDKGDIIGVLGSKPPHKMKE
EECCCCCEEEEEECCCCCEEEEEECCCCCCEEECCEEEEECCCCCEEEECCCCCCHHHHH
EERNKLMKSEFMFIDIGAKNKEDAINMGVEIGSWISFKTEFDVLGNNRITGKAFDDRAGC
HHHHHHHHHCEEEEEECCCCCCCHHHCCEEECCEEEEEEEEEEECCCEEECCCCCCCCCH
AVLLDVAKKLKDMELNCQLYLVGTVQEEVGLKGAKTSAFGISPDVAFALDVTICGDHPGI
HHHHHHHHHHHCCCCCEEEEEEEEEHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCC
TMEDAAVEMGKGPVFGIVDAAGRGLIAHPKVITMVKEVSKKYDIPVQYEVGDGGTTDATS
EECHHHHHCCCCCEEEEEECCCCCEEECCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEE
IQLTQDGVPTGVISVPSRYIHTPVEMIDIEDLEKTSDLMLKCVVDLNNYFE
EEEECCCCCCCEEECCHHHHCCCHHEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]