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The map label for this gene is cobD

Identifier: 150401135

GI number: 150401135

Start: 723867

End: 724802

Strand: Direct

Name: cobD

Synonym: Maeo_0706

Alternate gene names: 150401135

Gene position: 723867-724802 (Clockwise)

Preceding gene: 150401134

Following gene: 150401142

Centisome position: 46.12

GC content: 27.03

Gene sequence:

>936_bases
ATGTTAAGCCCGATTATTTTATTTTTATCAATTATTATTGATAGGATTTTTGGGGAGCTCCCTGAAAAAATACACCCTAC
CGTATGGATAGGTAATATCATATCTTTTTTTGAAAAAATATTGAAATCCACCCATTCAAAAAATAAATATAAAGATTTTA
TATTTGGAACCCTTACCACAATATCCGTATTATTTATAGTATTTGGAGCAATTTACGGAGTTGAAATATTAATAAACAAC
ATACAAAATATTTATATTAAATATATAGTTTATTCTTTTCTTATTTCCACCACAATAGGCTATAAATCATTATTGCAGTT
CTCTAAAACTCCATTAAACCATATCAAAAACAAAGATATTGAAAGTGCAAAAAAATCAGTTCAATGTATTGTAAGCAGAA
ATACGGACAAATTAGATACAAAACATATATTGTCTGCATCAATTGAAAGTGCATCAGAAAATATAACCGATAGTATTATA
GCCCCATTATTTTATGCCATTTTCTTTGGATTAGAGGGGGCTTTTATTTACCGTGCCATAAATACAATGGATGCAATGTT
AGGTTATAGAAATAAAAAATATGAATATTATGGAAAATTACCTGCAATTTTAGACGATATAGCAAATTTTATACCTTCCC
GAATTAGCGGTATTTTGTTGGTATTATTTGCACCATTATACGGCGGAAACATTAAAAAGGCACTAAATGGATTTATAAAA
GAAGGTCATAAAACACCATCACCAAATTCAGGCTACACAATGGCAGTAATGGCAAATAGTCTAAATATGACCTTGGAAAA
AATAGGATATTATAAATTAGGAAATGGAGAAATAACATTAAAAAAAGCATATAATTCATTATTTTCAATAGATGTTGTAA
TTTTCTCCTTTATAGTGTTGTATAGTATATATTATGTTATTTTTTATTATTTTTAA

Upstream 100 bases:

>100_bases
TAGATATCGAAAAAATTGAGAAATACAACACCATAAAGAAAAAAAGAACAAAAAAATTCCTCAATGCCCTTAGTAAATTA
TAAATATTCAGTGATTGCCT

Downstream 100 bases:

>100_bases
TATCTCAAAACTCCTTTGGAGTTTTTACGATCGTAAAATTCCTTTGGAATTTGAGATTTTATTTATCCTCCTCCACCCCC
ATATATTTAACAACCAATTC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLSPIILFLSIIIDRIFGELPEKIHPTVWIGNIISFFEKILKSTHSKNKYKDFIFGTLTTISVLFIVFGAIYGVEILINN
IQNIYIKYIVYSFLISTTIGYKSLLQFSKTPLNHIKNKDIESAKKSVQCIVSRNTDKLDTKHILSASIESASENITDSII
APLFYAIFFGLEGAFIYRAINTMDAMLGYRNKKYEYYGKLPAILDDIANFIPSRISGILLVLFAPLYGGNIKKALNGFIK
EGHKTPSPNSGYTMAVMANSLNMTLEKIGYYKLGNGEITLKKAYNSLFSIDVVIFSFIVLYSIYYVIFYYF

Sequences:

>Translated_311_residues
MLSPIILFLSIIIDRIFGELPEKIHPTVWIGNIISFFEKILKSTHSKNKYKDFIFGTLTTISVLFIVFGAIYGVEILINN
IQNIYIKYIVYSFLISTTIGYKSLLQFSKTPLNHIKNKDIESAKKSVQCIVSRNTDKLDTKHILSASIESASENITDSII
APLFYAIFFGLEGAFIYRAINTMDAMLGYRNKKYEYYGKLPAILDDIANFIPSRISGILLVLFAPLYGGNIKKALNGFIK
EGHKTPSPNSGYTMAVMANSLNMTLEKIGYYKLGNGEITLKKAYNSLFSIDVVIFSFIVLYSIYYVIFYYF
>Mature_311_residues
MLSPIILFLSIIIDRIFGELPEKIHPTVWIGNIISFFEKILKSTHSKNKYKDFIFGTLTTISVLFIVFGAIYGVEILINN
IQNIYIKYIVYSFLISTTIGYKSLLQFSKTPLNHIKNKDIESAKKSVQCIVSRNTDKLDTKHILSASIESASENITDSII
APLFYAIFFGLEGAFIYRAINTMDAMLGYRNKKYEYYGKLPAILDDIANFIPSRISGILLVLFAPLYGGNIKKALNGFIK
EGHKTPSPNSGYTMAVMANSLNMTLEKIGYYKLGNGEITLKKAYNSLFSIDVVIFSFIVLYSIYYVIFYYF

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_META3 (A6UUW7)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324901.1
- STRING:   A6UUW7
- GeneID:   5326947
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0706
- eggNOG:   arNOG05935
- HOGENOM:   HBG647131
- OMA:   MINPIYL
- ProtClustDB:   PRK01209
- BioCyc:   MAEO419665:MAEO_0706-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 35251; Mature: 35251

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b0d2d9c)-; HASH(0x1eedfa08)-; HASH(0x1f29e0f8)-; HASH(0x1eea80a8)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPIILFLSIIIDRIFGELPEKIHPTVWIGNIISFFEKILKSTHSKNKYKDFIFGTLTT
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ISVLFIVFGAIYGVEILINNIQNIYIKYIVYSFLISTTIGYKSLLQFSKTPLNHIKNKDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCH
ESAKKSVQCIVSRNTDKLDTKHILSASIESASENITDSIIAPLFYAIFFGLEGAFIYRAI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NTMDAMLGYRNKKYEYYGKLPAILDDIANFIPSRISGILLVLFAPLYGGNIKKALNGFIK
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EGHKTPSPNSGYTMAVMANSLNMTLEKIGYYKLGNGEITLKKAYNSLFSIDVVIFSFIVL
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHH
YSIYYVIFYYF
HHHHHHHHHCH
>Mature Secondary Structure
MLSPIILFLSIIIDRIFGELPEKIHPTVWIGNIISFFEKILKSTHSKNKYKDFIFGTLTT
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ISVLFIVFGAIYGVEILINNIQNIYIKYIVYSFLISTTIGYKSLLQFSKTPLNHIKNKDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCH
ESAKKSVQCIVSRNTDKLDTKHILSASIESASENITDSIIAPLFYAIFFGLEGAFIYRAI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NTMDAMLGYRNKKYEYYGKLPAILDDIANFIPSRISGILLVLFAPLYGGNIKKALNGFIK
HHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EGHKTPSPNSGYTMAVMANSLNMTLEKIGYYKLGNGEITLKKAYNSLFSIDVVIFSFIVL
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHH
YSIYYVIFYYF
HHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA