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The map label for this gene is gatB

Identifier: 150400875

GI number: 150400875

Start: 463447

End: 464856

Strand: Direct

Name: gatB

Synonym: Maeo_0443

Alternate gene names: 150400875

Gene position: 463447-464856 (Clockwise)

Preceding gene: 150400874

Following gene: 150400877

Centisome position: 29.53

GC content: 34.68

Gene sequence:

>1410_bases
ATGGATGAAACAGTATCAATGAAATGCGGTTTAGAAATACATGTGCAAGTAGATACAAATTCTAAATTATTTTGCACATG
CCCTACAAATTACAACGAAGTAGAACCAAACACAAATATATGTCCTGTTTGTATGGCTCACCCCGGGGCAAGACCCATGC
CACCAAACAAAAAAGCAGTAGATATGGCAATAATGGTTGCCAAGATGCTTGGTTGTGAGATTGTATTGGATAAAGATATT
TACTTCCAAAGAAAACACTATAATTACCCAGATTTGCCAAGCGGATACCAAAGAACTTCTGTTCCAGTTGGGGAGCATGG
TAAATTTTTAGGTGTTGGAATTACAGAGGTTCATTTGGAGGAAGACCCAGGACAATATAAACCAGATTTAGGAGTTGTAG
ATTATAATAGAAGTGGAACTCCACTAATAGAAATCGTATCCGACCCAGATATAAAAAGCCCAGAGGAAGCAAAAGAGTTT
TTAAGACAGCTTTTGAGATTATTTAATTATATTGGAAATTTAAGGGGAGAGGGCTCTATGAGGGCTGATGTAAATATATC
TGTTAATTATAATGGAGTTCAAGGCAATAGGGTAGAGGTAAAAAATGTTAATTCCATCAAAGGAGTTTATAAAGTTTTAA
AATATGAATTTATTAGGCAAAAAAACATATTAAGAAGAGGCGGAGAGATAAAAAGAGAGACTCGAGCATTTATGGAAGCT
CAACTTATCACAAAAGCTATGAGAAGTAAAGAAACCGCAGACGATTACAGGCATATTCCAGACCCCGACCTTCAACCCAT
TGTAATAAATAAGGAATGGGTAAAAACAGTTGAGGAAAAGATGCCAGAAACCCCAATGGACAAAGAAAAAAGATTTGTAG
AGCAATATGGCATAAAATTAGAAGATGCTAAGGTTATGGTTGCCGACCTTGCCCTTGCCGATGTTTTTGAAACTGTTGTA
AGTGAATTGGGTAAGGAAAAAGAAAATATATCTCTTGCCGTGATTTGGATAAGAAATGAATTAAGAAGGGTATTAGCATA
TAATAATATAGATTTCCTTGAAAGCAGATTAAAACCAGAACATATAACAGAATTAATACAGTTAATCACCGGAAAAACCA
TAAGCCAAAAAATTGGTAAGAAAGTTATAGAGATTATGGTAGAAAATAAGGGAGAAAAAACACCAAAACAAATAATTAAG
GAGTTAGGTTTAACAGTTATCGATTCTGAGGCCGTTCTTGAAACAGCATGTAAAGAAGCCATTGAAAATAATCCAAAAGC
TGTCGATGATTATTTAACTGGAAATGAGGGGGCTCTTAACTTTGTAGTTGGTCAAGTTATGAGAGCAACCAGAGGAAGGG
CACAACCAAATAAAGTTGTTGAAATATTGAAAAAATTAATAAATAAATAA

Upstream 100 bases:

>100_bases
TAATAATAATAATAATAAAATAATAAAATAATAAAATATAAAACATATATTAAGAGATACAATAGAGATATAATCTATAA
ATTATAATTTCGGTGATTAA

Downstream 100 bases:

>100_bases
TTATTTTCTTTTTTACTTTTTTATGATTATTAACAAGGAGCTCCAATATAATCACACTCCAATATAATCACAATATAACT
ATTGAAATATTCGGGAGCTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MDETVSMKCGLEIHVQVDTNSKLFCTCPTNYNEVEPNTNICPVCMAHPGARPMPPNKKAVDMAIMVAKMLGCEIVLDKDI
YFQRKHYNYPDLPSGYQRTSVPVGEHGKFLGVGITEVHLEEDPGQYKPDLGVVDYNRSGTPLIEIVSDPDIKSPEEAKEF
LRQLLRLFNYIGNLRGEGSMRADVNISVNYNGVQGNRVEVKNVNSIKGVYKVLKYEFIRQKNILRRGGEIKRETRAFMEA
QLITKAMRSKETADDYRHIPDPDLQPIVINKEWVKTVEEKMPETPMDKEKRFVEQYGIKLEDAKVMVADLALADVFETVV
SELGKEKENISLAVIWIRNELRRVLAYNNIDFLESRLKPEHITELIQLITGKTISQKIGKKVIEIMVENKGEKTPKQIIK
ELGLTVIDSEAVLETACKEAIENNPKAVDDYLTGNEGALNFVVGQVMRATRGRAQPNKVVEILKKLINK

Sequences:

>Translated_469_residues
MDETVSMKCGLEIHVQVDTNSKLFCTCPTNYNEVEPNTNICPVCMAHPGARPMPPNKKAVDMAIMVAKMLGCEIVLDKDI
YFQRKHYNYPDLPSGYQRTSVPVGEHGKFLGVGITEVHLEEDPGQYKPDLGVVDYNRSGTPLIEIVSDPDIKSPEEAKEF
LRQLLRLFNYIGNLRGEGSMRADVNISVNYNGVQGNRVEVKNVNSIKGVYKVLKYEFIRQKNILRRGGEIKRETRAFMEA
QLITKAMRSKETADDYRHIPDPDLQPIVINKEWVKTVEEKMPETPMDKEKRFVEQYGIKLEDAKVMVADLALADVFETVV
SELGKEKENISLAVIWIRNELRRVLAYNNIDFLESRLKPEHITELIQLITGKTISQKIGKKVIEIMVENKGEKTPKQIIK
ELGLTVIDSEAVLETACKEAIENNPKAVDDYLTGNEGALNFVVGQVMRATRGRAQPNKVVEILKKLINK
>Mature_469_residues
MDETVSMKCGLEIHVQVDTNSKLFCTCPTNYNEVEPNTNICPVCMAHPGARPMPPNKKAVDMAIMVAKMLGCEIVLDKDI
YFQRKHYNYPDLPSGYQRTSVPVGEHGKFLGVGITEVHLEEDPGQYKPDLGVVDYNRSGTPLIEIVSDPDIKSPEEAKEF
LRQLLRLFNYIGNLRGEGSMRADVNISVNYNGVQGNRVEVKNVNSIKGVYKVLKYEFIRQKNILRRGGEIKRETRAFMEA
QLITKAMRSKETADDYRHIPDPDLQPIVINKEWVKTVEEKMPETPMDKEKRFVEQYGIKLEDAKVMVADLALADVFETVV
SELGKEKENISLAVIWIRNELRRVLAYNNIDFLESRLKPEHITELIQLITGKTISQKIGKKVIEIMVENKGEKTPKQIIK
ELGLTVIDSEAVLETACKEAIENNPKAVDDYLTGNEGALNFVVGQVMRATRGRAQPNKVVEILKKLINK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=33.8056680161943, Blast_Score=252, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=37.7926421404682, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=27.831094049904, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=30.142566191446, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_META3 (A6UU57)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324641.1
- ProteinModelPortal:   A6UU57
- SMR:   A6UU57
- STRING:   A6UU57
- GeneID:   5326380
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0443
- eggNOG:   arNOG04834
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   MAEO419665:MAEO_0443-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53102; Mature: 53102

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDETVSMKCGLEIHVQVDTNSKLFCTCPTNYNEVEPNTNICPVCMAHPGARPMPPNKKAV
CCCCEEEEECEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHH
DMAIMVAKMLGCEIVLDKDIYFQRKHYNYPDLPSGYQRTSVPVGEHGKFLGVGITEVHLE
HHHHHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECEEEEEEE
EDPGQYKPDLGVVDYNRSGTPLIEIVSDPDIKSPEEAKEFLRQLLRLFNYIGNLRGEGSM
CCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCE
RADVNISVNYNGVQGNRVEVKNVNSIKGVYKVLKYEFIRQKNILRRGGEIKRETRAFMEA
EEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QLITKAMRSKETADDYRHIPDPDLQPIVINKEWVKTVEEKMPETPMDKEKRFVEQYGIKL
HHHHHHHHCCCCHHHHCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHCCEE
EDAKVMVADLALADVFETVVSELGKEKENISLAVIWIRNELRRVLAYNNIDFLESRLKPE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCHHHHHHCCCHH
HITELIQLITGKTISQKIGKKVIEIMVENKGEKTPKQIIKELGLTVIDSEAVLETACKEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECHHHHHHHHHHHH
IENNPKAVDDYLTGNEGALNFVVGQVMRATRGRAQPNKVVEILKKLINK
HHCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDETVSMKCGLEIHVQVDTNSKLFCTCPTNYNEVEPNTNICPVCMAHPGARPMPPNKKAV
CCCCEEEEECEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHH
DMAIMVAKMLGCEIVLDKDIYFQRKHYNYPDLPSGYQRTSVPVGEHGKFLGVGITEVHLE
HHHHHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECEEEEEEE
EDPGQYKPDLGVVDYNRSGTPLIEIVSDPDIKSPEEAKEFLRQLLRLFNYIGNLRGEGSM
CCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCE
RADVNISVNYNGVQGNRVEVKNVNSIKGVYKVLKYEFIRQKNILRRGGEIKRETRAFMEA
EEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
QLITKAMRSKETADDYRHIPDPDLQPIVINKEWVKTVEEKMPETPMDKEKRFVEQYGIKL
HHHHHHHHCCCCHHHHCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHCCEE
EDAKVMVADLALADVFETVVSELGKEKENISLAVIWIRNELRRVLAYNNIDFLESRLKPE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCHHHHHHCCCHH
HITELIQLITGKTISQKIGKKVIEIMVENKGEKTPKQIIKELGLTVIDSEAVLETACKEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEECHHHHHHHHHHHH
IENNPKAVDDYLTGNEGALNFVVGQVMRATRGRAQPNKVVEILKKLINK
HHCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA