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The map label for this gene is rffG [C]

Identifier: 150400860

GI number: 150400860

Start: 446206

End: 447237

Strand: Direct

Name: rffG [C]

Synonym: Maeo_0428

Alternate gene names: 150400860

Gene position: 446206-447237 (Clockwise)

Preceding gene: 150400859

Following gene: 150400861

Centisome position: 28.43

GC content: 29.17

Gene sequence:

>1032_bases
ATGAATAAATCTCAATTGGAAAATTTAAAATCCTTTTATGACAATAAGGTTATTCTAGTAACGGGAGGAACTGGCTCAAT
AGGAAAAGAAATTGTAAAAACTTTATTAAATTTTAATCCAAAGGCGATTAGAGTTTTAGATATAAATGAAACAGCACTTT
TCGATTTAGAACAAGAACTAAACACTCCTAAAATAAGGGCATTTTTAGGTGATATAAGAGATAAGGATAGATTAAAAAGA
GCTGTTGAAGGGGTAGATATAATATTCCATGCAGCAGCATTAAAACATGTTCCCCTATGTGAATATAATCCATTCGAAGC
AGTAAAAACAAATGTAATAGGTACTCAAAATTTAATAGATGTTGCGATGGATGAAGAGGTTGAAAAATTCATAACAGTAA
GCACGGATAAGGCAGTCAATCCAGTAAATGTTATGGGTGCAACTAAATTATTAGCCGAAAGATTAACCACTTCTGCGAAC
TTATATAAAGGACATAGAAAAACTGCTTTTTCTGTTGTTAGATTTGGAAATGTTTTAAATTCGAGAGGTTCAATTTTACC
ATTGTTAAAAGAACAATTAAAAAATGGAAAGGATATTACCTTAACACATCCTGAGATGACGAGATTTATAATGAGTATCA
ACGATGCTGTAAAATTAGTTTTAAAATCATGCACATTGGCAAAAGGTGGAGAAATATTCATATTAAAAATGCCTTCTGTA
AAAATTAAAGATTTAATAGAAGTGGTTGTTGAAGAGCTCGCTCCAAAATATGGATATGATCCAGAAGAGATAAATATTAA
AATAATTGGTAAAAGACCGGGCGAAAAATTATATGAAGAGTTGATAGTAGAAGAAGAAATTTATAAATTGGAAGAGTTAG
AGGATATGTTTGTTGTTTATCCTTATAAGTCAAATACAGATAATAAAGATAAAAAAATAATTTATGATTCAAAAAATACT
AAATTTTTAAGTAAGGGGGAAATTAAGAGAGTATTGTTTGATATTAGATACCTGGGTGATAATTATGAATGA

Upstream 100 bases:

>100_bases
TTCAAAAATATTGACACTCCCCATATATCCAAACATGACTAAAAATGAATTAAATTATATCATTGATACAATAAAAGAAT
TTTTTGAAGGGCGATGACTT

Downstream 100 bases:

>100_bases
TAAGGATACATTTACCAACTACATCATAAACAATTTTCCAAATATAATACCAATTTTATCCAATAACTTTCCATTTAAAT
TTCCAAAAGTTAAGTATGTT

Product: polysaccharide biosynthesis protein CapD

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNKSQLENLKSFYDNKVILVTGGTGSIGKEIVKTLLNFNPKAIRVLDINETALFDLEQELNTPKIRAFLGDIRDKDRLKR
AVEGVDIIFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIDVAMDEEVEKFITVSTDKAVNPVNVMGATKLLAERLTTSAN
LYKGHRKTAFSVVRFGNVLNSRGSILPLLKEQLKNGKDITLTHPEMTRFIMSINDAVKLVLKSCTLAKGGEIFILKMPSV
KIKDLIEVVVEELAPKYGYDPEEINIKIIGKRPGEKLYEELIVEEEIYKLEELEDMFVVYPYKSNTDNKDKKIIYDSKNT
KFLSKGEIKRVLFDIRYLGDNYE

Sequences:

>Translated_343_residues
MNKSQLENLKSFYDNKVILVTGGTGSIGKEIVKTLLNFNPKAIRVLDINETALFDLEQELNTPKIRAFLGDIRDKDRLKR
AVEGVDIIFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIDVAMDEEVEKFITVSTDKAVNPVNVMGATKLLAERLTTSAN
LYKGHRKTAFSVVRFGNVLNSRGSILPLLKEQLKNGKDITLTHPEMTRFIMSINDAVKLVLKSCTLAKGGEIFILKMPSV
KIKDLIEVVVEELAPKYGYDPEEINIKIIGKRPGEKLYEELIVEEEIYKLEELEDMFVVYPYKSNTDNKDKKIIYDSKNT
KFLSKGEIKRVLFDIRYLGDNYE
>Mature_343_residues
MNKSQLENLKSFYDNKVILVTGGTGSIGKEIVKTLLNFNPKAIRVLDINETALFDLEQELNTPKIRAFLGDIRDKDRLKR
AVEGVDIIFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIDVAMDEEVEKFITVSTDKAVNPVNVMGATKLLAERLTTSAN
LYKGHRKTAFSVVRFGNVLNSRGSILPLLKEQLKNGKDITLTHPEMTRFIMSINDAVKLVLKSCTLAKGGEIFILKMPSV
KIKDLIEVVVEELAPKYGYDPEEINIKIIGKRPGEKLYEELIVEEEIYKLEELEDMFVVYPYKSNTDNKDKKIIYDSKNT
KFLSKGEIKRVLFDIRYLGDNYE

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=200, Percent_Identity=28, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8393516, Length=200, Percent_Identity=28, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI7657641, Length=265, Percent_Identity=27.5471698113208, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38894; Mature: 38894

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSQLENLKSFYDNKVILVTGGTGSIGKEIVKTLLNFNPKAIRVLDINETALFDLEQEL
CCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCHHHC
NTPKIRAFLGDIRDKDRLKRAVEGVDIIFHAAALKHVPLCEYNPFEAVKTNVIGTQNLID
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHH
VAMDEEVEKFITVSTDKAVNPVNVMGATKLLAERLTTSANLYKGHRKTAFSVVRFGNVLN
HHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
SRGSILPLLKEQLKNGKDITLTHPEMTRFIMSINDAVKLVLKSCTLAKGGEIFILKMPSV
CCCCCHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
KIKDLIEVVVEELAPKYGYDPEEINIKIIGKRPGEKLYEELIVEEEIYKLEELEDMFVVY
CHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
PYKSNTDNKDKKIIYDSKNTKFLSKGEIKRVLFDIRYLGDNYE
EECCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKSQLENLKSFYDNKVILVTGGTGSIGKEIVKTLLNFNPKAIRVLDINETALFDLEQEL
CCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCCHHHC
NTPKIRAFLGDIRDKDRLKRAVEGVDIIFHAAALKHVPLCEYNPFEAVKTNVIGTQNLID
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHH
VAMDEEVEKFITVSTDKAVNPVNVMGATKLLAERLTTSANLYKGHRKTAFSVVRFGNVLN
HHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
SRGSILPLLKEQLKNGKDITLTHPEMTRFIMSINDAVKLVLKSCTLAKGGEIFILKMPSV
CCCCCHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
KIKDLIEVVVEELAPKYGYDPEEINIKIIGKRPGEKLYEELIVEEEIYKLEELEDMFVVY
CHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
PYKSNTDNKDKKIIYDSKNTKFLSKGEIKRVLFDIRYLGDNYE
EECCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]