Click here to switch to the map view.

The map label for this gene is spsK [H]

Identifier: 150400814

GI number: 150400814

Start: 393892

End: 394809

Strand: Direct

Name: spsK [H]

Synonym: Maeo_0381

Alternate gene names: 150400814

Gene position: 393892-394809 (Clockwise)

Preceding gene: 150400813

Following gene: 150400815

Centisome position: 25.1

GC content: 30.39

Gene sequence:

>918_bases
ATGGAAAAAATAGCAATTATTGGTTTGGGAATGATTGGATATGAAATCACTAAAAAATATAATGAATTGGGATATGATAC
ACATATTATAAATCGTAGTGATAAAGGTTTTTTTAAAAATAATGATAATATAACTAATCATTTTGTAGATATTACCGACG
AAAATAAAATAAAAAAAACCATTGAAAATATAAATCCTGATTTTGTTGTAAATACGGCGGCATTTACCAATGTGGATTTA
TGCGAAACTGAGAAGGAGCAGGCATATAAAACCAACGCTCTTTCTGTTGGATATGTGGGAGCTCCCTGTAAAAAATTAAA
TATTCCATTGTGCCATATATCCACTGACTATGTTTTTGATGGGGAAGATGGTAATTATGTGGAAAATGATGAAATAAATC
CAATAAACTATTATGGATATACAAAAGCTGAGGGGGAAAAGATTTTAAATGAATTAAATCATGATTTAACCTCGATTGTT
AGAATTTCAGTTCCTTACTGCATAAGTCCTGTTAAAGTCAATTTTTTTATGTGGGTATTGGACATGCTTAAAAAAGGAGA
GGATATGAATATTTTGATTGACCAGTGGAATACCCCAACATTCATAAATGAATTGGTTGATGGTATTGTGATAATTCATA
AAAAAGATGCTTCTGGGTTGTTTCATTTTGGAGGTGGCGAAAAAGTAAGCAGATATGAATTTGCCTTAAAGGTTGCCGAA
ATATTTGAAATGGATAAAACCCCAATAAATCCTGTTGAAAGCTCTGAAATGAATTGGAAAGCAAATAGACCAAAAGATAC
GACTTTAAATAATGGTAAAATCGAACGAAAATTAAAAATAAAATTGAAAACTGTTGATGAATGTTTAAAGGAAATTAAAG
GGAGCTCCTATAAATTTATATCGGAGCTCCACCACTAA

Upstream 100 bases:

>100_bases
TTTGAAGATGCATTAAAATATACTGTAAAATGGTATTTGGAAAATAAATGGTTTTGGGAATAATTAATTTTTACCCAAAA
TTATTTAATTTGGTGGAATT

Downstream 100 bases:

>100_bases
ATCACAACTATTATATAACATGTCCAACATATTTGACATAATAACGATAGGGTGTCAAATATGATAGACAAAAAAATAAT
ATTTGAAGAAGTAATATTAG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEKIAIIGLGMIGYEITKKYNELGYDTHIINRSDKGFFKNNDNITNHFVDITDENKIKKTIENINPDFVVNTAAFTNVDL
CETEKEQAYKTNALSVGYVGAPCKKLNIPLCHISTDYVFDGEDGNYVENDEINPINYYGYTKAEGEKILNELNHDLTSIV
RISVPYCISPVKVNFFMWVLDMLKKGEDMNILIDQWNTPTFINELVDGIVIIHKKDASGLFHFGGGEKVSRYEFALKVAE
IFEMDKTPINPVESSEMNWKANRPKDTTLNNGKIERKLKIKLKTVDECLKEIKGSSYKFISELHH

Sequences:

>Translated_305_residues
MEKIAIIGLGMIGYEITKKYNELGYDTHIINRSDKGFFKNNDNITNHFVDITDENKIKKTIENINPDFVVNTAAFTNVDL
CETEKEQAYKTNALSVGYVGAPCKKLNIPLCHISTDYVFDGEDGNYVENDEINPINYYGYTKAEGEKILNELNHDLTSIV
RISVPYCISPVKVNFFMWVLDMLKKGEDMNILIDQWNTPTFINELVDGIVIIHKKDASGLFHFGGGEKVSRYEFALKVAE
IFEMDKTPINPVESSEMNWKANRPKDTTLNNGKIERKLKIKLKTVDECLKEIKGSSYKFISELHH
>Mature_305_residues
MEKIAIIGLGMIGYEITKKYNELGYDTHIINRSDKGFFKNNDNITNHFVDITDENKIKKTIENINPDFVVNTAAFTNVDL
CETEKEQAYKTNALSVGYVGAPCKKLNIPLCHISTDYVFDGEDGNYVENDEINPINYYGYTKAEGEKILNELNHDLTSIV
RISVPYCISPVKVNFFMWVLDMLKKGEDMNILIDQWNTPTFINELVDGIVIIHKKDASGLFHFGGGEKVSRYEFALKVAE
IFEMDKTPINPVESSEMNWKANRPKDTTLNNGKIERKLKIKLKTVDECLKEIKGSSYKFISELHH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=277, Percent_Identity=26.3537906137184, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=25.8741258741259, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1788352, Length=288, Percent_Identity=29.5138888888889, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 34839; Mature: 34839

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIAIIGLGMIGYEITKKYNELGYDTHIINRSDKGFFKNNDNITNHFVDITDENKIKKT
CCEEEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHH
IENINPDFVVNTAAFTNVDLCETEKEQAYKTNALSVGYVGAPCKKLNIPLCHISTDYVFD
HHCCCCCEEEEEEEECCCCHHCCCHHHHHHCCCEEECCCCCCHHHCCCEEEEEEECEEEE
GEDGNYVENDEINPINYYGYTKAEGEKILNELNHDLTSIVRISVPYCISPVKVNFFMWVL
CCCCCEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCEECCHHHHHHHHHH
DMLKKGEDMNILIDQWNTPTFINELVDGIVIIHKKDASGLFHFGGGEKVSRYEFALKVAE
HHHHCCCCCEEEEECCCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHH
IFEMDKTPINPVESSEMNWKANRPKDTTLNNGKIERKLKIKLKTVDECLKEIKGSSYKFI
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHCCCCHHHH
SELHH
HHHCC
>Mature Secondary Structure
MEKIAIIGLGMIGYEITKKYNELGYDTHIINRSDKGFFKNNDNITNHFVDITDENKIKKT
CCEEEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHH
IENINPDFVVNTAAFTNVDLCETEKEQAYKTNALSVGYVGAPCKKLNIPLCHISTDYVFD
HHCCCCCEEEEEEEECCCCHHCCCHHHHHHCCCEEECCCCCCHHHCCCEEEEEEECEEEE
GEDGNYVENDEINPINYYGYTKAEGEKILNELNHDLTSIVRISVPYCISPVKVNFFMWVL
CCCCCEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCEECCHHHHHHHHHH
DMLKKGEDMNILIDQWNTPTFINELVDGIVIIHKKDASGLFHFGGGEKVSRYEFALKVAE
HHHHCCCCCEEEEECCCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHH
IFEMDKTPINPVESSEMNWKANRPKDTTLNNGKIERKLKIKLKTVDECLKEIKGSSYKFI
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEHHHHHHHHHHCCCCHHHH
SELHH
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]