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The map label for this gene is leuD [H]

Identifier: 150400780

GI number: 150400780

Start: 369137

End: 369643

Strand: Direct

Name: leuD [H]

Synonym: Maeo_0347

Alternate gene names: 150400780

Gene position: 369137-369643 (Clockwise)

Preceding gene: 150400779

Following gene: 150400781

Centisome position: 23.52

GC content: 33.14

Gene sequence:

>507_bases
ATGGATAAAACAATAGAAGGAAAAGTATGGAAATTTGGAGATAATATCGATACAGATGCAATATTACCTGCAAGATACTT
AGTTTATACCACAGAAGAAGAATTGGCAAAATTTGTAATGGCTGGAACAGACCCAGAATTTGCTGAAAAAGTGGAAAAAG
GAGACATCATAATAGGGGGAACAAACTTTGGCTCTGGTTCAAGTAGGGAACATGCACCAATGGGATTAAAAGGAGCAGGT
ATATCTTTGGTAATAGCTGAAAGTTTTGCAAGAATATTTTACAGAAATTCAATAAATATCGGGCTTCCATTATTGGAATG
TAAAGACATCACAAAACATGTGAATGAAGGAGATGTTTTACAAATAAATTTAGACGAAGGAACCATAATAAACAAAACAA
CAGGAAAAGAGTTAAAAGGTCAAAAACTTCCCGAGTTTATGATGGATATACTTAATGATGGCGGATTAATGCCACATTTA
AAAAAGAAGTTATCGTCTAATGAATAA

Upstream 100 bases:

>100_bases
TCTAATTATAATAAATATCGTAAGATGGCATCAGACATTTAAGAACTCACTTTCCGAACAGAATATATATTAACATTATT
CAAAGATAAAAGGCGATATA

Downstream 100 bases:

>100_bases
ATAACTATATTATTTTTTTATATGGTGGAGCTCCCGAATATATATACCATATTTTTTAATTTTTTAAATTGGTGATTAAT
TAATGAAAAAACATTTGAAT

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MDKTIEGKVWKFGDNIDTDAILPARYLVYTTEEELAKFVMAGTDPEFAEKVEKGDIIIGGTNFGSGSSREHAPMGLKGAG
ISLVIAESFARIFYRNSINIGLPLLECKDITKHVNEGDVLQINLDEGTIINKTTGKELKGQKLPEFMMDILNDGGLMPHL
KKKLSSNE

Sequences:

>Translated_168_residues
MDKTIEGKVWKFGDNIDTDAILPARYLVYTTEEELAKFVMAGTDPEFAEKVEKGDIIIGGTNFGSGSSREHAPMGLKGAG
ISLVIAESFARIFYRNSINIGLPLLECKDITKHVNEGDVLQINLDEGTIINKTTGKELKGQKLPEFMMDILNDGGLMPHL
KKKLSSNE
>Mature_168_residues
MDKTIEGKVWKFGDNIDTDAILPARYLVYTTEEELAKFVMAGTDPEFAEKVEKGDIIIGGTNFGSGSSREHAPMGLKGAG
ISLVIAESFARIFYRNSINIGLPLLECKDITKHVNEGDVLQINLDEGTIINKTTGKELKGQKLPEFMMDILNDGGLMPHL
KKKLSSNE

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=98, Percent_Identity=37.7551020408163, Blast_Score=66, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320440, Length=106, Percent_Identity=45.2830188679245, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=144, Percent_Identity=36.8055555555556, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 18459; Mature: 18459

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTIEGKVWKFGDNIDTDAILPARYLVYTTEEELAKFVMAGTDPEFAEKVEKGDIIIGG
CCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCHHHHHHHCCCCEEEEC
TNFGSGSSREHAPMGLKGAGISLVIAESFARIFYRNSINIGLPLLECKDITKHVNEGDVL
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEE
QINLDEGTIINKTTGKELKGQKLPEFMMDILNDGGLMPHLKKKLSSNE
EEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MDKTIEGKVWKFGDNIDTDAILPARYLVYTTEEELAKFVMAGTDPEFAEKVEKGDIIIGG
CCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCHHHHHHHCCCCEEEEC
TNFGSGSSREHAPMGLKGAGISLVIAESFARIFYRNSINIGLPLLECKDITKHVNEGDVL
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEE
QINLDEGTIINKTTGKELKGQKLPEFMMDILNDGGLMPHLKKKLSSNE
EEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]