Click here to switch to the map view.

The map label for this gene is hacA [H]

Identifier: 150400745

GI number: 150400745

Start: 336478

End: 337743

Strand: Direct

Name: hacA [H]

Synonym: Maeo_0311

Alternate gene names: 150400745

Gene position: 336478-337743 (Clockwise)

Preceding gene: 150400744

Following gene: 150400767

Centisome position: 21.44

GC content: 33.97

Gene sequence:

>1266_bases
ATGACATTGGCAGAGGAAATATTATCAAAAAAAGTAGGAAAAAAAGTAAAAGCAGGAGATGTTGTAGAAATAGATATAGA
TTTAGCAATGACTCATGATGGAACAACACCATTATCTGCAAAGGCATTTAAACAGATAACCGATAAGGTATGGGACAATA
AAAAAATAGTCATAGTATTTGACCATAATGTCCCAGCAAATACATTAAAAGCCGCAAATATGCAAAAAATTACACGAGAA
TTTATAAAAGAACAAAATATAATAAATCATTATTTGGATGGTGAAGGCGTATGCCATCAAGTGCTACCTGAAAACGGACA
TATACAACCAAACATGGTTATAGCTGGTGGAGATAGTCACACATGCACCTATGGGGCATTTGGGGCATTTGCAACAGGAT
TTGGGGCTACCGACATGGGGAATATATATGCAACAGGAAAAACTTGGTTAAAAGTTCCAAAAACCATAAGAATAAATGTT
AATGGAGAAAATGATAAAATTACTGGAAAAGATATTATTTTAAAAATTTGTAAAGAAGTTGGACGAAGTGGAGCTACTTA
CATGGCACTTGAATACGGCGGGGAAGCAATAAAAAAATTAAGTATGGACGAAAGAATGGTTTTAAGCAATATGGCTATCG
AAATGGGCGGAAAAGTTGGGCTTATCGAAGCCGATGAAACCACATATAATTACCTTAGAAATGTAGGAATTAGTGAAGAA
AAAATATTAGAATTGAAAAAAAATCAAATAACCATTGATGAGAACAATATAGATAATGATAATTATTATAAAATTATAAA
TATAGATATTACAGACATGGAGGAGCAAGTGGCATGCCCTCACCATCCCGATAATGTTAAAAATATTTCGGAAGTAAAAG
GAGCTCCCATAAATCAGGTGTTCATAGGTTCATGCACAAATGGTAGGTTGAACGATTTAAGAATAGCCTCAAAATATTTA
AAAGGAAAAAAGGTTCATAATGATGTTAGATTAATAGTAATACCTGCTTCAAAATCAATATTTAAACAGGCATTAAAAGA
AGGATTAATTGATATTTTTGTAGATGCTGGAGCTTTAATTTGCACCCCCGGATGCGGTCCTTGTTTGGGGGCCCACCAAG
GAGTTTTAGGAGATGGTGAAGTTTGTTTAGCCACCACAAATAGAAATTTCAAAGGAAGAATGGGAAATACGACAGCGGAA
ATATATTTATCCTCCCCCGCTATTGCCGCAAAAAGTGCAATTAAAGGATATATCACAAATGAATAA

Upstream 100 bases:

>100_bases
GATTGTGACTCGAACCATAGGTTTGATTTTCTTATTGGATATATAAGGACTTTAACTATTAATTAACAATATTTAAAATT
CAAAATAAACGAGGATAAAT

Downstream 100 bases:

>100_bases
AATAATAAATTTTGGGAGCTCCCAATTTATATTTTTTGCCCTACAATTATATTATCTTGCACCCTATTTATTTTAACATT
TACAGTTTTTCCTTCTAATT

Product: 3-isopropylmalate dehydratase

Products: NA

Alternate protein names: HACN; (R)-homocitrate dehydratase; Homoaconitate hydratase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTLAEEILSKKVGKKVKAGDVVEIDIDLAMTHDGTTPLSAKAFKQITDKVWDNKKIVIVFDHNVPANTLKAANMQKITRE
FIKEQNIINHYLDGEGVCHQVLPENGHIQPNMVIAGGDSHTCTYGAFGAFATGFGATDMGNIYATGKTWLKVPKTIRINV
NGENDKITGKDIILKICKEVGRSGATYMALEYGGEAIKKLSMDERMVLSNMAIEMGGKVGLIEADETTYNYLRNVGISEE
KILELKKNQITIDENNIDNDNYYKIINIDITDMEEQVACPHHPDNVKNISEVKGAPINQVFIGSCTNGRLNDLRIASKYL
KGKKVHNDVRLIVIPASKSIFKQALKEGLIDIFVDAGALICTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTTAE
IYLSSPAIAAKSAIKGYITNE

Sequences:

>Translated_421_residues
MTLAEEILSKKVGKKVKAGDVVEIDIDLAMTHDGTTPLSAKAFKQITDKVWDNKKIVIVFDHNVPANTLKAANMQKITRE
FIKEQNIINHYLDGEGVCHQVLPENGHIQPNMVIAGGDSHTCTYGAFGAFATGFGATDMGNIYATGKTWLKVPKTIRINV
NGENDKITGKDIILKICKEVGRSGATYMALEYGGEAIKKLSMDERMVLSNMAIEMGGKVGLIEADETTYNYLRNVGISEE
KILELKKNQITIDENNIDNDNYYKIINIDITDMEEQVACPHHPDNVKNISEVKGAPINQVFIGSCTNGRLNDLRIASKYL
KGKKVHNDVRLIVIPASKSIFKQALKEGLIDIFVDAGALICTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTTAE
IYLSSPAIAAKSAIKGYITNE
>Mature_420_residues
TLAEEILSKKVGKKVKAGDVVEIDIDLAMTHDGTTPLSAKAFKQITDKVWDNKKIVIVFDHNVPANTLKAANMQKITREF
IKEQNIINHYLDGEGVCHQVLPENGHIQPNMVIAGGDSHTCTYGAFGAFATGFGATDMGNIYATGKTWLKVPKTIRINVN
GENDKITGKDIILKICKEVGRSGATYMALEYGGEAIKKLSMDERMVLSNMAIEMGGKVGLIEADETTYNYLRNVGISEEK
ILELKKNQITIDENNIDNDNYYKIINIDITDMEEQVACPHHPDNVKNISEVKGAPINQVFIGSCTNGRLNDLRIASKYLK
GKKVHNDVRLIVIPASKSIFKQALKEGLIDIFVDAGALICTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTTAEI
YLSSPAIAAKSAIKGYITNE

Specific function: Hydro-lyase with broad substrate specificity for cis- unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4- tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4- tricarboxyla

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=351, Percent_Identity=27.0655270655271, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI8659555, Length=443, Percent_Identity=23.9277652370203, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI41352693, Length=390, Percent_Identity=22.0512820512821, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786259, Length=438, Percent_Identity=34.2465753424658, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI2367097, Length=434, Percent_Identity=25.3456221198157, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=26.2108262108262, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=22.4226804123711, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32564738, Length=355, Percent_Identity=30.4225352112676, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=355, Percent_Identity=30.4225352112676, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=295, Percent_Identity=30.8474576271186, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320440, Length=411, Percent_Identity=32.6034063260341, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=437, Percent_Identity=33.1807780320366, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6323335, Length=368, Percent_Identity=27.1739130434783, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=352, Percent_Identity=28.6931818181818, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571643, Length=375, Percent_Identity=28.5333333333333, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161076999, Length=376, Percent_Identity=27.3936170212766, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281365315, Length=376, Percent_Identity=27.3936170212766, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17864292, Length=376, Percent_Identity=27.3936170212766, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137564, Length=374, Percent_Identity=24.331550802139, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645686, Length=453, Percent_Identity=23.6203090507726, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.36 [H]

Molecular weight: Translated: 45904; Mature: 45773

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAEEILSKKVGKKVKAGDVVEIDIDLAMTHDGTTPLSAKAFKQITDKVWDNKKIVIVF
CCHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEE
DHNVPANTLKAANMQKITREFIKEQNIINHYLDGEGVCHQVLPENGHIQPNMVIAGGDSH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCCCC
TCTYGAFGAFATGFGATDMGNIYATGKTWLKVPKTIRINVNGENDKITGKDIILKICKEV
EEEECCHHHHHCCCCCCCCCCEEECCCEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHH
GRSGATYMALEYGGEAIKKLSMDERMVLSNMAIEMGGKVGLIEADETTYNYLRNVGISEE
CCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCHH
KILELKKNQITIDENNIDNDNYYKIINIDITDMEEQVACPHHPDNVKNISEVKGAPINQV
HHHHHHCCEEEEECCCCCCCCEEEEEEEEEECCHHHCCCCCCCCCCCCHHHHCCCCCCEE
FIGSCTNGRLNDLRIASKYLKGKKVHNDVRLIVIPASKSIFKQALKEGLIDIFVDAGALI
EEECCCCCCCHHHHHHHHHHCCCEECCCEEEEEEECCHHHHHHHHHHCCEEEEECCCEEE
CTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTTAEIYLSSPAIAAKSAIKGYITN
ECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHCCEECC
E
C
>Mature Secondary Structure 
TLAEEILSKKVGKKVKAGDVVEIDIDLAMTHDGTTPLSAKAFKQITDKVWDNKKIVIVF
CHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEE
DHNVPANTLKAANMQKITREFIKEQNIINHYLDGEGVCHQVLPENGHIQPNMVIAGGDSH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEECCCCC
TCTYGAFGAFATGFGATDMGNIYATGKTWLKVPKTIRINVNGENDKITGKDIILKICKEV
EEEECCHHHHHCCCCCCCCCCEEECCCEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHH
GRSGATYMALEYGGEAIKKLSMDERMVLSNMAIEMGGKVGLIEADETTYNYLRNVGISEE
CCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCHH
KILELKKNQITIDENNIDNDNYYKIINIDITDMEEQVACPHHPDNVKNISEVKGAPINQV
HHHHHHCCEEEEECCCCCCCCEEEEEEEEEECCHHHCCCCCCCCCCCCHHHHCCCCCCEE
FIGSCTNGRLNDLRIASKYLKGKKVHNDVRLIVIPASKSIFKQALKEGLIDIFVDAGALI
EEECCCCCCCHHHHHHHHHHCCCEECCCEEEEEEECCHHHHHHHHHHCCEEEEECCCEEE
CTPGCGPCLGAHQGVLGDGEVCLATTNRNFKGRMGNTTAEIYLSSPAIAAKSAIKGYITN
ECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHCCEECC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]