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The map label for this gene is leuC [H]

Identifier: 150400724

GI number: 150400724

Start: 312764

End: 314041

Strand: Reverse

Name: leuC [H]

Synonym: Maeo_0289

Alternate gene names: 150400724

Gene position: 314041-312764 (Counterclockwise)

Preceding gene: 150400728

Following gene: 150400714

Centisome position: 20.01

GC content: 38.5

Gene sequence:

>1278_bases
ATGGCAATGACATTAGCAGAAAAAATACTTGCAAAAGCATCAGGAAATAAAACAGTAGCTCCCGGAGACATAGTAATGGC
CAAAATAGAAACGGCAATGGCTCATGATATTACTGGACCATTGACAGTGAATACATTAAAAAATGAAGGAATAGAAAAAG
TATGGGATAATGAAGAGAAAATAACAATACTTTTCGACCACCAAATACCTGCGGATAGTATAACCGCAGCAGAAAACCAT
GCCCTTTTAAGAAAATTTGTAAAAGAACAAGGCATAAAAAACTTCTATGATATTAGAGAAGGAGTTTGCCATCAAGTTTT
ACCTGAAAAAGGACATGTTTTACCAGGAACCGTTGCAGTTGGAGCAGATTCCCACACTTGCACATATGGAGCATTTGGAA
CATTTGCAACAGGAATAGGAAGCACAGACATGGCAGCAGTATTTGCAACAGGAGAATTATGGTTTAAAGTACCTGAAACG
CTATACTTTAATGTAAGTGGAAAATTAAACGATTTCGTTACCTCAAAAGATGTAATATTAAAAATAATTGGAGAAGTTGG
ATTTGATGGCGCAACTTACAAAGCAGCCCAGTATGGCGGTGAAACAGTTGAAGAGATGTCCATTTCTTCAAGAATGACCA
TGTCAAATATGGCCATTGAAATGGGTGCAAAAGCTGGACTTATAGAACCAGATGTAAAAACAACATATTTCATAAAAAGG
GCTATGCAAAAGCACGGGACAACAAGACCATACACCGCACTAAAAGGAGACGAAGACGCTAAATTTGAAGAAACCTATGA
AATAGAAGTAAATGACTTAGAACCATTATTCGCTTGCCCTCATGCTGTGGATAATGTAAAAACTGCAAGAGAAGTTGCTG
GAAGACCTATTGACCAAGTATTTATCGGAAGTTGCACCAACGGAAGAGTTGAGGATTTAAGAGCTGCAATAAACATCATA
GACCAGCATGGAGGTATAGCTGATGATGTTAGGGTTGTAGTCACCCCAGCTTCAAGAGAGGTAATGTTGGAGGCAATTAA
CGAAGGAATTATTCAAAAATTCTATGAATATGGCTGTGTCGTAACAAATCCGTCATGTTCTGCATGTATGGGGGCCCTCT
ATGGTATATTGGGACCTAATGAAGTTGGGGTTGCTACCTCAAATAGAAATTTCAGAGGAAGAGAAGGTTCATTGGAATCA
GAAGTTTATTTGGCCTCACCAATCACAGCAGCAGCTTGTGCGGTTAAAGGGGAATTAATAGACCCAAGAGATTTATAA

Upstream 100 bases:

>100_bases
AATCCTTTTCCTTTGGATTTATAGATTTTCAAGGAAAGAGTTTTTTCCGAGCACACTATACCGTTTATTAAAAACAAAAT
AATTTAAATAGAGGCAAATT

Downstream 100 bases:

>100_bases
AAAAATAAATTTAATATCTTTATATTATTTTATATATTTTTTAACAATTATTTATTTATGTTTATTTTATATTTTGAAAC
TTCATCATCATATCCTAAAA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAMTLAEKILAKASGNKTVAPGDIVMAKIETAMAHDITGPLTVNTLKNEGIEKVWDNEEKITILFDHQIPADSITAAENH
ALLRKFVKEQGIKNFYDIREGVCHQVLPEKGHVLPGTVAVGADSHTCTYGAFGTFATGIGSTDMAAVFATGELWFKVPET
LYFNVSGKLNDFVTSKDVILKIIGEVGFDGATYKAAQYGGETVEEMSISSRMTMSNMAIEMGAKAGLIEPDVKTTYFIKR
AMQKHGTTRPYTALKGDEDAKFEETYEIEVNDLEPLFACPHAVDNVKTAREVAGRPIDQVFIGSCTNGRVEDLRAAINII
DQHGGIADDVRVVVTPASREVMLEAINEGIIQKFYEYGCVVTNPSCSACMGALYGILGPNEVGVATSNRNFRGREGSLES
EVYLASPITAAACAVKGELIDPRDL

Sequences:

>Translated_425_residues
MAMTLAEKILAKASGNKTVAPGDIVMAKIETAMAHDITGPLTVNTLKNEGIEKVWDNEEKITILFDHQIPADSITAAENH
ALLRKFVKEQGIKNFYDIREGVCHQVLPEKGHVLPGTVAVGADSHTCTYGAFGTFATGIGSTDMAAVFATGELWFKVPET
LYFNVSGKLNDFVTSKDVILKIIGEVGFDGATYKAAQYGGETVEEMSISSRMTMSNMAIEMGAKAGLIEPDVKTTYFIKR
AMQKHGTTRPYTALKGDEDAKFEETYEIEVNDLEPLFACPHAVDNVKTAREVAGRPIDQVFIGSCTNGRVEDLRAAINII
DQHGGIADDVRVVVTPASREVMLEAINEGIIQKFYEYGCVVTNPSCSACMGALYGILGPNEVGVATSNRNFRGREGSLES
EVYLASPITAAACAVKGELIDPRDL
>Mature_424_residues
AMTLAEKILAKASGNKTVAPGDIVMAKIETAMAHDITGPLTVNTLKNEGIEKVWDNEEKITILFDHQIPADSITAAENHA
LLRKFVKEQGIKNFYDIREGVCHQVLPEKGHVLPGTVAVGADSHTCTYGAFGTFATGIGSTDMAAVFATGELWFKVPETL
YFNVSGKLNDFVTSKDVILKIIGEVGFDGATYKAAQYGGETVEEMSISSRMTMSNMAIEMGAKAGLIEPDVKTTYFIKRA
MQKHGTTRPYTALKGDEDAKFEETYEIEVNDLEPLFACPHAVDNVKTAREVAGRPIDQVFIGSCTNGRVEDLRAAINIID
QHGGIADDVRVVVTPASREVMLEAINEGIIQKFYEYGCVVTNPSCSACMGALYGILGPNEVGVATSNRNFRGREGSLESE
VYLASPITAAACAVKGELIDPRDL

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=384, Percent_Identity=26.5625, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI8659555, Length=492, Percent_Identity=23.3739837398374, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI41352693, Length=391, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786259, Length=472, Percent_Identity=33.4745762711864, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI87081781, Length=429, Percent_Identity=23.7762237762238, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787531, Length=389, Percent_Identity=23.3933161953728, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367097, Length=132, Percent_Identity=34.0909090909091, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=384, Percent_Identity=27.6041666666667, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25149337, Length=384, Percent_Identity=27.6041666666667, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=27.112676056338, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17568399, Length=394, Percent_Identity=23.0964467005076, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321429, Length=453, Percent_Identity=35.3200883002208, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6320440, Length=449, Percent_Identity=30.9576837416481, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323335, Length=344, Percent_Identity=29.9418604651163, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=387, Percent_Identity=27.906976744186, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI28571643, Length=342, Percent_Identity=29.8245614035088, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=25.6696428571429, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=25.6696428571429, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161076999, Length=343, Percent_Identity=27.4052478134111, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17137564, Length=502, Percent_Identity=23.5059760956175, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24645686, Length=453, Percent_Identity=23.6203090507726, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 45904; Mature: 45773

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTLAEKILAKASGNKTVAPGDIVMAKIETAMAHDITGPLTVNTLKNEGIEKVWDNEEK
CCHHHHHHHHHHCCCCCEECCCCEEHHHHHHHHHHCCCCCEEEEEHHCCCCHHHCCCCCE
ITILFDHQIPADSITAAENHALLRKFVKEQGIKNFYDIREGVCHQVLPEKGHVLPGTVAV
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
GADSHTCTYGAFGTFATGIGSTDMAAVFATGELWFKVPETLYFNVSGKLNDFVTSKDVIL
CCCCCEEECCCHHHHHHCCCCCCCEEEEEECCEEEECCCEEEEEECCCHHHHHCHHHHHH
KIIGEVGFDGATYKAAQYGGETVEEMSISSRMTMSNMAIEMGAKAGLIEPDVKTTYFIKR
HHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AMQKHGTTRPYTALKGDEDAKFEETYEIEVNDLEPLFACPHAVDNVKTAREVAGRPIDQV
HHHHCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCCCE
FIGSCTNGRVEDLRAAINIIDQHGGIADDVRVVVTPASREVMLEAINEGIIQKFYEYGCV
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCE
VTNPSCSACMGALYGILGPNEVGVATSNRNFRGREGSLESEVYLASPITAAACAVKGELI
EECCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCC
DPRDL
CCCCC
>Mature Secondary Structure 
AMTLAEKILAKASGNKTVAPGDIVMAKIETAMAHDITGPLTVNTLKNEGIEKVWDNEEK
CHHHHHHHHHHCCCCCEECCCCEEHHHHHHHHHHCCCCCEEEEEHHCCCCHHHCCCCCE
ITILFDHQIPADSITAAENHALLRKFVKEQGIKNFYDIREGVCHQVLPEKGHVLPGTVAV
EEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
GADSHTCTYGAFGTFATGIGSTDMAAVFATGELWFKVPETLYFNVSGKLNDFVTSKDVIL
CCCCCEEECCCHHHHHHCCCCCCCEEEEEECCEEEECCCEEEEEECCCHHHHHCHHHHHH
KIIGEVGFDGATYKAAQYGGETVEEMSISSRMTMSNMAIEMGAKAGLIEPDVKTTYFIKR
HHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AMQKHGTTRPYTALKGDEDAKFEETYEIEVNDLEPLFACPHAVDNVKTAREVAGRPIDQV
HHHHCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCCCE
FIGSCTNGRVEDLRAAINIIDQHGGIADDVRVVVTPASREVMLEAINEGIIQKFYEYGCV
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCE
VTNPSCSACMGALYGILGPNEVGVATSNRNFRGREGSLESEVYLASPITAAACAVKGELI
EECCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCC
DPRDL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]