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The map label for this gene is proS

Identifier: 150400672

GI number: 150400672

Start: 258895

End: 260265

Strand: Direct

Name: proS

Synonym: Maeo_0234

Alternate gene names: 150400672

Gene position: 258895-260265 (Clockwise)

Preceding gene: 150400663

Following gene: 150400673

Centisome position: 16.5

GC content: 32.75

Gene sequence:

>1371_bases
ATGGAAAACTTTTCAGAATGGTATCATAATATACTTGAAACAGCTGGAATATATGATTTAAGATATCCTTTAAAAGGTTG
CGGTGTTTATCTCCCCTATGGATTTAAAATAAGAAGATATGCATTTGAAGCCATAAGAGATATGCTTGACGAATCAAACC
ACGATGAAGCACTATTCCCAATGTTAATTCCAGAAGATTTATTGGCAAAGGAAGGAGAACATATTAAAGGATTTGAGGAG
GAAGTTTATTGGGTTACACATGGGGGAACTACTCCACTTGATGTAAAACTTGCTTTAAGACCAACATCAGAAACTCCAAT
ATATCACATGATGAAATTATGGATAAAAGTTCATACAGATTTGCCAATAAAAATATATCAAATTGTAAATTCATTTAGAT
ATGAAACAAAGCACACCAGACCACTTATAAGATTAAGAGAAATAATGACCTTTAAAGAGGCACATACAGCACATTCCACT
AGCGAAGAGGCTGAGGCACAGGTGCAAACTGCACTAAATATATATAAAACATTCTTTGATAGGATGGGAGTTCCTACAAT
TGTATCGCAAAGACCAGAATGGGACAAATTCCCCGGTGCAGATTATACAATGGCTTTTGATACTATATTCCCCGACGGAA
AAACTATGCAAATTGGGACGGTGCATAATTTGGGGCAGCATTTTGCAAAAACTTTTGAATTGGAGTTTGAAACCCCGGAA
GGAGGAAAAGACTACGCATACCAAACATGCTATGGTATTTCAGATAGAATAATAGCTTCAATAATAGCACTACACGGAGA
TGAAAAAGGGCTTATATTGCCACCAGAAGTAGCTCCGCACCAGATTATAATTATTCCATTATTATTTAAAGGAAAAGAAG
AAATTGCAATGAATAAAGCAAAAGAAATATATAAATCACTTAAAAACACATACAGAGTAAAATTAGATGATAGAGATATA
AGACCAGGCAAGAAATTTAATGACTGGGAATTAAAGGGAGCTCCCATAAGGATAGAGTTGGGACCAAGAGATATTGAAAA
TAATAAATTAACAATTTACAGAAGAGATACAGGCGAAAAATTCCAAATAGATGAGGATAATTTATTAAATGAGCTAAATA
CTCTTATTGATTCAATAGAAAATACAATTAAAGAAAAGGCAGAACAGAAAGTTAAATCTTTTATTACAATATTGGATAAT
CATGATGTTAATAACATTAAAGAAACATTATCCACCAAAAAAGGAGTTGTATTGGTTCCTTATGACGAAAATATATATAC
TGAGGAATTTGAGGAAGAAATTGATGCTTCTGTATTGGGGACTACTGAATATGATGGGAAAAAGTATATTTCTATTGCTA
AAACATATTAA

Upstream 100 bases:

>100_bases
ATAATTATGATTATAACAATTATAAAATAATTATTATCATAATATTAAATATTTTTTTAATATGCAGTTAATTAAACTAT
ACGCATAAAACGGTGAAATG

Downstream 100 bases:

>100_bases
TTATTTTTTTATCTTATTTTTTATTAATTTTTGCAATGCTCTGCTGAATTATTCGTGGGGGCAGGTCAGAATAATATTTT
GGATTATTTTAATAATGTAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MENFSEWYHNILETAGIYDLRYPLKGCGVYLPYGFKIRRYAFEAIRDMLDESNHDEALFPMLIPEDLLAKEGEHIKGFEE
EVYWVTHGGTTPLDVKLALRPTSETPIYHMMKLWIKVHTDLPIKIYQIVNSFRYETKHTRPLIRLREIMTFKEAHTAHST
SEEAEAQVQTALNIYKTFFDRMGVPTIVSQRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQHFAKTFELEFETPE
GGKDYAYQTCYGISDRIIASIIALHGDEKGLILPPEVAPHQIIIIPLLFKGKEEIAMNKAKEIYKSLKNTYRVKLDDRDI
RPGKKFNDWELKGAPIRIELGPRDIENNKLTIYRRDTGEKFQIDEDNLLNELNTLIDSIENTIKEKAEQKVKSFITILDN
HDVNNIKETLSTKKGVVLVPYDENIYTEEFEEEIDASVLGTTEYDGKKYISIAKTY

Sequences:

>Translated_456_residues
MENFSEWYHNILETAGIYDLRYPLKGCGVYLPYGFKIRRYAFEAIRDMLDESNHDEALFPMLIPEDLLAKEGEHIKGFEE
EVYWVTHGGTTPLDVKLALRPTSETPIYHMMKLWIKVHTDLPIKIYQIVNSFRYETKHTRPLIRLREIMTFKEAHTAHST
SEEAEAQVQTALNIYKTFFDRMGVPTIVSQRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQHFAKTFELEFETPE
GGKDYAYQTCYGISDRIIASIIALHGDEKGLILPPEVAPHQIIIIPLLFKGKEEIAMNKAKEIYKSLKNTYRVKLDDRDI
RPGKKFNDWELKGAPIRIELGPRDIENNKLTIYRRDTGEKFQIDEDNLLNELNTLIDSIENTIKEKAEQKVKSFITILDN
HDVNNIKETLSTKKGVVLVPYDENIYTEEFEEEIDASVLGTTEYDGKKYISIAKTY
>Mature_456_residues
MENFSEWYHNILETAGIYDLRYPLKGCGVYLPYGFKIRRYAFEAIRDMLDESNHDEALFPMLIPEDLLAKEGEHIKGFEE
EVYWVTHGGTTPLDVKLALRPTSETPIYHMMKLWIKVHTDLPIKIYQIVNSFRYETKHTRPLIRLREIMTFKEAHTAHST
SEEAEAQVQTALNIYKTFFDRMGVPTIVSQRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQHFAKTFELEFETPE
GGKDYAYQTCYGISDRIIASIIALHGDEKGLILPPEVAPHQIIIIPLLFKGKEEIAMNKAKEIYKSLKNTYRVKLDDRDI
RPGKKFNDWELKGAPIRIELGPRDIENNKLTIYRRDTGEKFQIDEDNLLNELNTLIDSIENTIKEKAEQKVKSFITILDN
HDVNNIKETLSTKKGVVLVPYDENIYTEEFEEEIDASVLGTTEYDGKKYISIAKTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=444, Percent_Identity=35.1351351351351, Blast_Score=282, Evalue=4e-76,
Organism=Escherichia coli, GI1786392, Length=171, Percent_Identity=30.4093567251462, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71991342, Length=465, Percent_Identity=33.5483870967742, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI71991346, Length=361, Percent_Identity=36.01108033241, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321808, Length=444, Percent_Identity=33.3333333333333, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24649468, Length=443, Percent_Identity=34.0857787810384, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24649466, Length=443, Percent_Identity=34.0857787810384, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_META3 (A6UTK4)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324438.1
- ProteinModelPortal:   A6UTK4
- SMR:   A6UTK4
- STRING:   A6UTK4
- GeneID:   5326817
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0234
- eggNOG:   arNOG04466
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   MAEO419665:MAEO_0234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR015264
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09181 ProRS-C_2; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 52782; Mature: 52782

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFSEWYHNILETAGIYDLRYPLKGCGVYLPYGFKIRRYAFEAIRDMLDESNHDEALFP
CCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEC
MLIPEDLLAKEGEHIKGFEEEVYWVTHGGTTPLDVKLALRPTSETPIYHMMKLWIKVHTD
EECCHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
LPIKIYQIVNSFRYETKHTRPLIRLREIMTFKEAHTAHSTSEEAEAQVQTALNIYKTFFD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RMGVPTIVSQRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQHFAKTFELEFETPE
HCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCHHHHHHHHHHEEEEEEECCC
GGKDYAYQTCYGISDRIIASIIALHGDEKGLILPPEVAPHQIIIIPLLFKGKEEIAMNKA
CCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEEECCCHHHHHHHH
KEIYKSLKNTYRVKLDDRDIRPGKKFNDWELKGAPIRIELGPRDIENNKLTIYRRDTGEK
HHHHHHHHHCEEEEECCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCEEEEEECCCCCE
FQIDEDNLLNELNTLIDSIENTIKEKAEQKVKSFITILDNHDVNNIKETLSTKKGVVLVP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
YDENIYTEEFEEEIDASVLGTTEYDGKKYISIAKTY
CCCCCCHHHHHHHHCCCEECCCCCCCCEEEEEEECC
>Mature Secondary Structure
MENFSEWYHNILETAGIYDLRYPLKGCGVYLPYGFKIRRYAFEAIRDMLDESNHDEALFP
CCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEC
MLIPEDLLAKEGEHIKGFEEEVYWVTHGGTTPLDVKLALRPTSETPIYHMMKLWIKVHTD
EECCHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
LPIKIYQIVNSFRYETKHTRPLIRLREIMTFKEAHTAHSTSEEAEAQVQTALNIYKTFFD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RMGVPTIVSQRPEWDKFPGADYTMAFDTIFPDGKTMQIGTVHNLGQHFAKTFELEFETPE
HCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCHHHHHHHHHHEEEEEEECCC
GGKDYAYQTCYGISDRIIASIIALHGDEKGLILPPEVAPHQIIIIPLLFKGKEEIAMNKA
CCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEEECCCHHHHHHHH
KEIYKSLKNTYRVKLDDRDIRPGKKFNDWELKGAPIRIELGPRDIENNKLTIYRRDTGEK
HHHHHHHHHCEEEEECCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCEEEEEECCCCCE
FQIDEDNLLNELNTLIDSIENTIKEKAEQKVKSFITILDNHDVNNIKETLSTKKGVVLVP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
YDENIYTEEFEEEIDASVLGTTEYDGKKYISIAKTY
CCCCCCHHHHHHHHCCCEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA