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The map label for this gene is rimK [H]

Identifier: 150400574

GI number: 150400574

Start: 153636

End: 154433

Strand: Direct

Name: rimK [H]

Synonym: Maeo_0136

Alternate gene names: 150400574

Gene position: 153636-154433 (Clockwise)

Preceding gene: 150400573

Following gene: 150400576

Centisome position: 9.79

GC content: 24.06

Gene sequence:

>798_bases
ATGATTTATGAAAAATTAAAAAACAATAATTATGCAGTATCATTTTTAGACCCATTGGAAATGATTTCTGGATTAAATAA
TAATTATAATAATTATAATATAATTTTATCACGGGTAGAAAGAGATTATTTAAAAGAGGGAATTTATGCCTTAAAATATC
TTGAAACGATACCAAATATTAAAATTATTAATAATTCTGATAGTATAGAAACATGTCAAAATAAATATTTAACTTATTTA
AAATTAAAAGAAGTAATGCCCAAATCATTTTTAACATATGCCAACGACTTTAAAAATGTTGAAAACGAACTGAAAGCAAA
TAATTTTAATTTTCCTGTGGTTGTAAAGCCAATATATGGCGGTTATGGAAATGGTGTTTTAAAAGTTAATAATTTAGGGG
AATTAAATAATATATTTGAATTGCTAAAAAATAATGGTAATGAATTATTTGTGCAGGAGCTCCTTGATTTTAAACATGAC
ATAAGGGCTTTTGTAATAAATAATAAAATAATTTGTGCAATGGAAAGAATACCTTCAAATAATGATTGGAGGGCAAATTA
TTCTCGAGGAGCTAAAATTAAAGAATTTAAATTAAATAAAGATACTGAAAAATTAATTTTAAATTCTGTAAAAAAAATAG
GGGCCAATATCGTTGGCGTAGATGTATTAATTGATAAAAATAATAAACCTTATATTTTGGAAATGAATATCACGCCACAA
TTTAGGGGAATTATGAATTTTGCAGATGTTCCAGGGGAGATTTTAAAATATTTGGCTGATATTTTGAATAAAAAATAA

Upstream 100 bases:

>100_bases
GGAGATTTGGAAAGTATAACTATAAAATAATACCTATGGTGAAAACATTAAAAAAACAAAAATTGCCATACTTTGCCACA
AAAGAACACCAGAAAACGAA

Downstream 100 bases:

>100_bases
AATAATAATAAAATAATATATTATAAAATTCTTTCAATTGGTAGCACAAGTATATCTTTTGCTCCTATTAATTCTAATTC
TGAAACTATTTTAAATACCT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MIYEKLKNNNYAVSFLDPLEMISGLNNNYNNYNIILSRVERDYLKEGIYALKYLETIPNIKIINNSDSIETCQNKYLTYL
KLKEVMPKSFLTYANDFKNVENELKANNFNFPVVVKPIYGGYGNGVLKVNNLGELNNIFELLKNNGNELFVQELLDFKHD
IRAFVINNKIICAMERIPSNNDWRANYSRGAKIKEFKLNKDTEKLILNSVKKIGANIVGVDVLIDKNNKPYILEMNITPQ
FRGIMNFADVPGEILKYLADILNKK

Sequences:

>Translated_265_residues
MIYEKLKNNNYAVSFLDPLEMISGLNNNYNNYNIILSRVERDYLKEGIYALKYLETIPNIKIINNSDSIETCQNKYLTYL
KLKEVMPKSFLTYANDFKNVENELKANNFNFPVVVKPIYGGYGNGVLKVNNLGELNNIFELLKNNGNELFVQELLDFKHD
IRAFVINNKIICAMERIPSNNDWRANYSRGAKIKEFKLNKDTEKLILNSVKKIGANIVGVDVLIDKNNKPYILEMNITPQ
FRGIMNFADVPGEILKYLADILNKK
>Mature_265_residues
MIYEKLKNNNYAVSFLDPLEMISGLNNNYNNYNIILSRVERDYLKEGIYALKYLETIPNIKIINNSDSIETCQNKYLTYL
KLKEVMPKSFLTYANDFKNVENELKANNFNFPVVVKPIYGGYGNGVLKVNNLGELNNIFELLKNNGNELFVQELLDFKHD
IRAFVINNKIICAMERIPSNNDWRANYSRGAKIKEFKLNKDTEKLILNSVKKIGANIVGVDVLIDKNNKPYILEMNITPQ
FRGIMNFADVPGEILKYLADILNKK

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=274, Percent_Identity=20.4379562043796, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 30596; Mature: 30596

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYEKLKNNNYAVSFLDPLEMISGLNNNYNNYNIILSRVERDYLKEGIYALKYLETIPNI
CCCHHHCCCCEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
KIINNSDSIETCQNKYLTYLKLKEVMPKSFLTYANDFKNVENELKANNFNFPVVVKPIYG
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GYGNGVLKVNNLGELNNIFELLKNNGNELFVQELLDFKHDIRAFVINNKIICAMERIPSN
CCCCCEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHEEEEECCEEEEEEECCCCC
NDWRANYSRGAKIKEFKLNKDTEKLILNSVKKIGANIVGVDVLIDKNNKPYILEMNITPQ
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCHH
FRGIMNFADVPGEILKYLADILNKK
HHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYEKLKNNNYAVSFLDPLEMISGLNNNYNNYNIILSRVERDYLKEGIYALKYLETIPNI
CCCHHHCCCCEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
KIINNSDSIETCQNKYLTYLKLKEVMPKSFLTYANDFKNVENELKANNFNFPVVVKPIYG
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GYGNGVLKVNNLGELNNIFELLKNNGNELFVQELLDFKHDIRAFVINNKIICAMERIPSN
CCCCCEEEECCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHEEEEECCEEEEEEECCCCC
NDWRANYSRGAKIKEFKLNKDTEKLILNSVKKIGANIVGVDVLIDKNNKPYILEMNITPQ
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCHH
FRGIMNFADVPGEILKYLADILNKK
HHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA