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The map label for this gene is nth [C]

Identifier: 150400428

GI number: 150400428

Start: 1709079

End: 1710149

Strand: Reverse

Name: nth [C]

Synonym: Mevan_1691

Alternate gene names: 150400428

Gene position: 1710149-1709079 (Counterclockwise)

Preceding gene: NA

Following gene: 150400427

Centisome position: 99.42

GC content: 29.41

Gene sequence:

>1071_bases
ATGGACCCGATTGAAAGTAATTTTGATGGATTTTTAGATATTTTAAGCGAAAAATTAAAAAAAGATGCAGTTGTAGATCA
AATTTCTAAAAATTCAACTTTTGAAGAACGGGCATTTAAAATTTTAGTTTCCACAAGTTTAAGCGCAAGAACAAAAGATG
AAACTACTGCAAAAGTCTCAAAAAATCTTTTTAGAGTAATACAAAATCCAGAAGACCTTTTAAATATTCCAATTAACGAG
TTAGAAAAGCTTGTTTATCCGGCTGGCTTTTATAAAACAAAATCTAAAAACTTAAAAGAACTTGGAAAAATCTTGGTTGA
AAAGTACAATTCAAAAATCCCAAATTCAATCGATGAACTTGTAAAACTTCCAGGAGTCGGTAGAAAAACTGCAAACCTAG
TAATGACTCTTGCATTTAGTGAAGATGCAATATGTGTTGATACGCACGTTCATAGAATTACAAATCGTTTAAATTACGTA
GATACGAAAAACCCTAATGAAACAGAAATGGCCCTAAGAAAAAAGCTTCCAAAAAAATACTGGAAACAAATAAATAACAG
TTTAGTAATTTTCGGGCAAGATATATGTGGTTTTGTTCCAAAATGCAGTTCTTGTTTTCCCGAGATTAAAAAAATATGCC
CTTATTATAGTTCGATTTTAGAAATTTTAAAGATTTATGAAAAATATAATTTTAAAAAGGTTTCAAAAACAAAAATTCCA
AGAGAACGTGGAACTTACGTGTTAAGAATAAAGATAAATAATTCAAAGAATATTTTAGTTGGGAAAAAAGAAATTCAATT
TGAAAAAGGAGATTATTTTTATATCGGTTCTGCAATGGGCAATTCATTAAATCTTTATAACCGGATAAACCGCCACTTAT
CGGATGAAAAAAAGAAACGGTGGCATATAGATTATTTACTGGAATTTGGAAACATTAAGGAAATATATGTTACAGATGGC
AGTTTTGAGTGCAGTGTTTCAAAAGATTTTGAAGCTATTCTAAAGGGAATTTTTGGCTTTGGATGTAGCGATTGCAAATG
TAAAAGCCACCTTTACTTTTTAAAACCATAA

Upstream 100 bases:

>100_bases
TCGATAAATAAATATAAAATGGATAAATAAGATACGTGATTAATTTTACACATTATAATTTTTATTTTAAGTAGTATAAA
AAATCTTTTTGGAGATAAAA

Downstream 100 bases:

>100_bases
ATTTAAAAATAAAATTTTTAAAATATTGCTTTTTTTAGTTTGCAAAAAGTATTTTACAAAAAGTATAAATCTTTGAAAAA
TAGAATATTTGTTCATGTTT

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDPIESNFDGFLDILSEKLKKDAVVDQISKNSTFEERAFKILVSTSLSARTKDETTAKVSKNLFRVIQNPEDLLNIPINE
LEKLVYPAGFYKTKSKNLKELGKILVEKYNSKIPNSIDELVKLPGVGRKTANLVMTLAFSEDAICVDTHVHRITNRLNYV
DTKNPNETEMALRKKLPKKYWKQINNSLVIFGQDICGFVPKCSSCFPEIKKICPYYSSILEILKIYEKYNFKKVSKTKIP
RERGTYVLRIKINNSKNILVGKKEIQFEKGDYFYIGSAMGNSLNLYNRINRHLSDEKKKRWHIDYLLEFGNIKEIYVTDG
SFECSVSKDFEAILKGIFGFGCSDCKCKSHLYFLKP

Sequences:

>Translated_356_residues
MDPIESNFDGFLDILSEKLKKDAVVDQISKNSTFEERAFKILVSTSLSARTKDETTAKVSKNLFRVIQNPEDLLNIPINE
LEKLVYPAGFYKTKSKNLKELGKILVEKYNSKIPNSIDELVKLPGVGRKTANLVMTLAFSEDAICVDTHVHRITNRLNYV
DTKNPNETEMALRKKLPKKYWKQINNSLVIFGQDICGFVPKCSSCFPEIKKICPYYSSILEILKIYEKYNFKKVSKTKIP
RERGTYVLRIKINNSKNILVGKKEIQFEKGDYFYIGSAMGNSLNLYNRINRHLSDEKKKRWHIDYLLEFGNIKEIYVTDG
SFECSVSKDFEAILKGIFGFGCSDCKCKSHLYFLKP
>Mature_356_residues
MDPIESNFDGFLDILSEKLKKDAVVDQISKNSTFEERAFKILVSTSLSARTKDETTAKVSKNLFRVIQNPEDLLNIPINE
LEKLVYPAGFYKTKSKNLKELGKILVEKYNSKIPNSIDELVKLPGVGRKTANLVMTLAFSEDAICVDTHVHRITNRLNYV
DTKNPNETEMALRKKLPKKYWKQINNSLVIFGQDICGFVPKCSSCFPEIKKICPYYSSILEILKIYEKYNFKKVSKTKIP
RERGTYVLRIKINNSKNILVGKKEIQFEKGDYFYIGSAMGNSLNLYNRINRHLSDEKKKRWHIDYLLEFGNIKEIYVTDG
SFECSVSKDFEAILKGIFGFGCSDCKCKSHLYFLKP

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=219, Percent_Identity=38.3561643835616, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1787920, Length=178, Percent_Identity=32.5842696629214, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=42.8571428571429, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319304, Length=184, Percent_Identity=35.3260869565217, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=182, Percent_Identity=34.0659340659341, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=43.8888888888889, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR002837
- InterPro:   IPR004036
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR000305 [H]

Pfam domain/function: PF01986 DUF123; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 4.2.99.18

Molecular weight: Translated: 41004; Mature: 41004

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIESNFDGFLDILSEKLKKDAVVDQISKNSTFEERAFKILVSTSLSARTKDETTAKVS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCHHHHHHH
KNLFRVIQNPEDLLNIPINELEKLVYPAGFYKTKSKNLKELGKILVEKYNSKIPNSIDEL
HHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VKLPGVGRKTANLVMTLAFSEDAICVDTHVHRITNRLNYVDTKNPNETEMALRKKLPKKY
HHCCCCCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
WKQINNSLVIFGQDICGFVPKCSSCFPEIKKICPYYSSILEILKIYEKYNFKKVSKTKIP
HHHHCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
RERGTYVLRIKINNSKNILVGKKEIQFEKGDYFYIGSAMGNSLNLYNRINRHLSDEKKKR
HHHCCEEEEEEECCCCEEEEEHHHEEEECCCEEEEECCCCCCHHHHHHHHHHHCHHHHHH
WHIDYLLEFGNIKEIYVTDGSFECSVSKDFEAILKGIFGFGCSDCKCKSHLYFLKP
EEHHHEEECCCEEEEEEECCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MDPIESNFDGFLDILSEKLKKDAVVDQISKNSTFEERAFKILVSTSLSARTKDETTAKVS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCHHHHHHH
KNLFRVIQNPEDLLNIPINELEKLVYPAGFYKTKSKNLKELGKILVEKYNSKIPNSIDEL
HHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
VKLPGVGRKTANLVMTLAFSEDAICVDTHVHRITNRLNYVDTKNPNETEMALRKKLPKKY
HHCCCCCCCCCEEEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
WKQINNSLVIFGQDICGFVPKCSSCFPEIKKICPYYSSILEILKIYEKYNFKKVSKTKIP
HHHHCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
RERGTYVLRIKINNSKNILVGKKEIQFEKGDYFYIGSAMGNSLNLYNRINRHLSDEKKKR
HHHCCEEEEEEECCCCEEEEEHHHEEEECCCEEEEECCCCCCHHHHHHHHHHHCHHHHHH
WHIDYLLEFGNIKEIYVTDGSFECSVSKDFEAILKGIFGFGCSDCKCKSHLYFLKP
EEHHHEEECCCEEEEEEECCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carbon-Oxygen Lyase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]