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The map label for this gene is sepS [H]

Identifier: 150400307

GI number: 150400307

Start: 1581697

End: 1583310

Strand: Reverse

Name: sepS [H]

Synonym: Mevan_1570

Alternate gene names: 150400307

Gene position: 1583310-1581697 (Counterclockwise)

Preceding gene: 150400309

Following gene: 150400304

Centisome position: 92.05

GC content: 32.03

Gene sequence:

>1614_bases
ATGTTTAAACGAGAAGAAATCATCGAAATGGCAAATAAGGACTTTGAAAAGGCTTGGATAGAAACTAAAGGCCTCATAAA
GTCTAAACGAGTAAATGAAAGTTATCCGAGAATTAAACCAATTTTCGGAAAGACACACCCTGTAAACGATACTATTGAAA
ATTTAAGGCAAGCATATCTTAGAATGGGGTTTGAAGAATACATAAATCCAGTAATTGTTGATGAAAGGGATATTTATAAA
CAGTTTGGCCCAGAAGCTATGGCAGTGTTAGATAGGTGCTTTTACCTAGCAGGCCTCCCAAGGCCAGACGTTGGATTAAG
TGATGAAAAAATTTCCCAAATTGAAAAACTTGGAATTAATGTATCTTGCCACAAGGAAAGCTTGCAAAAAATACTTCATG
GATATAAAAAAGGAACTCTTGATGGCGATGATTTAGTTTTAGAGATTTCAAAAGCTCTTGAAATTTCAAGTGAAATGGGT
TTAAAAATTTTAGAAGAAGTTTTCCCAGAATTTAAAGATTTAATTGCGGTTTCTTCAAAATTAACTTTAAGAAGCCATAT
GACTTCAGGTTGGTTTATAACTCTTTCAGAACTTAATGGTAAAAAACCGCTTCCTTTTAAGCTATTTTCAATCGACAGGT
GCTTTAGAAGGGAACAAAAAGAAGATAAAAGTCATTTAATGACTTATCACTCTGCATCGTGCGTAATTGCTGGAAAAGAT
GTAGATATTAACGATGGAAAGGCAGTTGCAGAAGGACTTTTGTCACAGTTTGGATTTACAAACTTTAAATTCATTCCTGA
TGAAAAAAAGAGTAAGTATTATACTCCAGAAACTCAGACTGAAGTTTATGCATACCATCCAAAATTAAAAGAATGGCTTG
AAGTTGCAACTTTTGGAGTATATTCACCAGTTGCACTTAGTAAATATGGAATAGATGTTCCGGTAATGAATTTGGGGCTT
GGTGTTGAACGACTTTCAATGATATCTGGAAATTTCGAAGATGTACGTGAAATGGTATATCCCCAGTTTTACGAGCAGAG
CTTATCTGATAGAGCTATTTCATCAATGGTAAAATTCGATAAAGTCCCAGTACTGGACGAAATTTACGATTTAACGAAAG
AATTAATTGATTTATGCGTTAAAAACAAAGATATAACATCGCCTTGTAACCTTAAATTAGAAAAAACGTTTATTTTTGGA
AAAACAAAGAAAAATGTTAAAATCATTGTATTTGAAAAAGAAGAAAACAAAAAATTACTTGGCCCTTCAATTTTAAACGA
GATTTACGTTTATGATGGAAATATTATCGGCATTCCAGAAACTTTTGAAGGGGTAAAGGAAGAATTTAAAGAATTTTTAG
AAAAAGGAAAAGTTGAAGGAGTAACTACCGGTATTCGATACATTGATGCATTGTGCTTTAAAATTACATCTAAAGTAGAA
GAAGCATTTGTTTCAAATACTAGTGAATTTAAATTAAAAGTTCCAATTGTTAGAAGTTTGAGCGACATTAACTTAAAAAT
AGAAGAAATCGCATTAAAACAGATCATGAGTAAAAATAAAGTTATCGATGTAAGGGGGCCCGTATTTTTAAATGTTGAAG
TGAAAATTGAATAA

Upstream 100 bases:

>100_bases
GTCACCCCGATTAATTAATATATAATATTGGCATTTATTATGATATAAAGATTACATTACACATTACTACCTATATAAAT
TCAGGATTTCAGTGATAATA

Downstream 100 bases:

>100_bases
TAAATTACATAAAAAATTTATATTTATTAAAAGTATTTATCTTAAAAAAGTTAGTGATCGGGCTTAAAATCCAATCAACT
TTCCAATATTATATGCCATA

Product: O-phosphoseryl-tRNA synthetase

Products: NA

Alternate protein names: SepRS; Non-canonical O-phosphoseryl-tRNA(Cys) synthetase; O-phosphoserine--tRNA(Cys) ligase; O-phosphoserine--tRNA ligase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MFKREEIIEMANKDFEKAWIETKGLIKSKRVNESYPRIKPIFGKTHPVNDTIENLRQAYLRMGFEEYINPVIVDERDIYK
QFGPEAMAVLDRCFYLAGLPRPDVGLSDEKISQIEKLGINVSCHKESLQKILHGYKKGTLDGDDLVLEISKALEISSEMG
LKILEEVFPEFKDLIAVSSKLTLRSHMTSGWFITLSELNGKKPLPFKLFSIDRCFRREQKEDKSHLMTYHSASCVIAGKD
VDINDGKAVAEGLLSQFGFTNFKFIPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGVYSPVALSKYGIDVPVMNLGL
GVERLSMISGNFEDVREMVYPQFYEQSLSDRAISSMVKFDKVPVLDEIYDLTKELIDLCVKNKDITSPCNLKLEKTFIFG
KTKKNVKIIVFEKEENKKLLGPSILNEIYVYDGNIIGIPETFEGVKEEFKEFLEKGKVEGVTTGIRYIDALCFKITSKVE
EAFVSNTSEFKLKVPIVRSLSDINLKIEEIALKQIMSKNKVIDVRGPVFLNVEVKIE

Sequences:

>Translated_537_residues
MFKREEIIEMANKDFEKAWIETKGLIKSKRVNESYPRIKPIFGKTHPVNDTIENLRQAYLRMGFEEYINPVIVDERDIYK
QFGPEAMAVLDRCFYLAGLPRPDVGLSDEKISQIEKLGINVSCHKESLQKILHGYKKGTLDGDDLVLEISKALEISSEMG
LKILEEVFPEFKDLIAVSSKLTLRSHMTSGWFITLSELNGKKPLPFKLFSIDRCFRREQKEDKSHLMTYHSASCVIAGKD
VDINDGKAVAEGLLSQFGFTNFKFIPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGVYSPVALSKYGIDVPVMNLGL
GVERLSMISGNFEDVREMVYPQFYEQSLSDRAISSMVKFDKVPVLDEIYDLTKELIDLCVKNKDITSPCNLKLEKTFIFG
KTKKNVKIIVFEKEENKKLLGPSILNEIYVYDGNIIGIPETFEGVKEEFKEFLEKGKVEGVTTGIRYIDALCFKITSKVE
EAFVSNTSEFKLKVPIVRSLSDINLKIEEIALKQIMSKNKVIDVRGPVFLNVEVKIE
>Mature_537_residues
MFKREEIIEMANKDFEKAWIETKGLIKSKRVNESYPRIKPIFGKTHPVNDTIENLRQAYLRMGFEEYINPVIVDERDIYK
QFGPEAMAVLDRCFYLAGLPRPDVGLSDEKISQIEKLGINVSCHKESLQKILHGYKKGTLDGDDLVLEISKALEISSEMG
LKILEEVFPEFKDLIAVSSKLTLRSHMTSGWFITLSELNGKKPLPFKLFSIDRCFRREQKEDKSHLMTYHSASCVIAGKD
VDINDGKAVAEGLLSQFGFTNFKFIPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGVYSPVALSKYGIDVPVMNLGL
GVERLSMISGNFEDVREMVYPQFYEQSLSDRAISSMVKFDKVPVLDEIYDLTKELIDLCVKNKDITSPCNLKLEKTFIFG
KTKKNVKIIVFEKEENKKLLGPSILNEIYVYDGNIIGIPETFEGVKEEFKEFLEKGKVEGVTTGIRYIDALCFKITSKVE
EAFVSNTSEFKLKVPIVRSLSDINLKIEEIALKQIMSKNKVIDVRGPVFLNVEVKIE

Specific function: Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys) [H]

COG id: COG2024

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit (archaeal type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=191, Percent_Identity=34.0314136125654, Blast_Score=93, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI32563657, Length=165, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17508957, Length=168, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321087, Length=164, Percent_Identity=32.9268292682927, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18858079, Length=140, Percent_Identity=33.5714285714286, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR005246
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.27 [H]

Molecular weight: Translated: 61381; Mature: 61381

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKREEIIEMANKDFEKAWIETKGLIKSKRVNESYPRIKPIFGKTHPVNDTIENLRQAYL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RMGFEEYINPVIVDERDIYKQFGPEAMAVLDRCFYLAGLPRPDVGLSDEKISQIEKLGIN
HCCHHHHCCCEEECCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
VSCHKESLQKILHGYKKGTLDGDDLVLEISKALEISSEMGLKILEEVFPEFKDLIAVSSK
EEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLRSHMTSGWFITLSELNGKKPLPFKLFSIDRCFRREQKEDKSHLMTYHSASCVIAGKD
HHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCC
VDINDGKAVAEGLLSQFGFTNFKFIPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGV
CCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCC
YSPVALSKYGIDVPVMNLGLGVERLSMISGNFEDVREMVYPQFYEQSLSDRAISSMVKFD
CCCHHHHHCCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KVPVLDEIYDLTKELIDLCVKNKDITSPCNLKLEKTFIFGKTKKNVKIIVFEKEENKKLL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCC
GPSILNEIYVYDGNIIGIPETFEGVKEEFKEFLEKGKVEGVTTGIRYIDALCFKITSKVE
CHHHHHEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EAFVSNTSEFKLKVPIVRSLSDINLKIEEIALKQIMSKNKVIDVRGPVFLNVEVKIE
HHHHCCCCCEEEEEHHHHCHHHCCCHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEC
>Mature Secondary Structure
MFKREEIIEMANKDFEKAWIETKGLIKSKRVNESYPRIKPIFGKTHPVNDTIENLRQAYL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RMGFEEYINPVIVDERDIYKQFGPEAMAVLDRCFYLAGLPRPDVGLSDEKISQIEKLGIN
HCCHHHHCCCEEECCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
VSCHKESLQKILHGYKKGTLDGDDLVLEISKALEISSEMGLKILEEVFPEFKDLIAVSSK
EEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLRSHMTSGWFITLSELNGKKPLPFKLFSIDRCFRREQKEDKSHLMTYHSASCVIAGKD
HHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCC
VDINDGKAVAEGLLSQFGFTNFKFIPDEKKSKYYTPETQTEVYAYHPKLKEWLEVATFGV
CCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCC
YSPVALSKYGIDVPVMNLGLGVERLSMISGNFEDVREMVYPQFYEQSLSDRAISSMVKFD
CCCHHHHHCCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KVPVLDEIYDLTKELIDLCVKNKDITSPCNLKLEKTFIFGKTKKNVKIIVFEKEENKKLL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCC
GPSILNEIYVYDGNIIGIPETFEGVKEEFKEFLEKGKVEGVTTGIRYIDALCFKITSKVE
CHHHHHEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EAFVSNTSEFKLKVPIVRSLSDINLKIEEIALKQIMSKNKVIDVRGPVFLNVEVKIE
HHHHCCCCCEEEEEHHHHCHHHCCCHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA