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The map label for this gene is ilvB [H]

Identifier: 150400280

GI number: 150400280

Start: 1556255

End: 1558018

Strand: Reverse

Name: ilvB [H]

Synonym: Mevan_1543

Alternate gene names: 150400280

Gene position: 1558018-1556255 (Counterclockwise)

Preceding gene: 150400284

Following gene: 150400279

Centisome position: 90.58

GC content: 37.59

Gene sequence:

>1764_bases
ATGAAAGGAGCAGAGGCTATGATGAAAGCCCTAAAAGCGGAAAATGTAAAAGTCCTCTTTGGATATCCAGGAGGTCAGTT
ACTTCCCTTTTATGATGCACTATACCACACAGATTTTGTACATATTTTAACAAGACATGAACAGGCCGCAGCCCATGCAG
CCGATGGATATGCAAGAGCGAGTGGTGACGTTGGAGTATGTGTGGCTACATCGGGACCTGGAGCTACAAACCTTGTTACA
GGAGTTGCAACGGCCCATGCAGATTCTTCACCAGTTGTCGCATTAACCGGGCAAGTTCCGACCAGATTAATAGGAAATGA
TGCATTTCAGGAAATTGATGCACTTGGAATTTTTATGCAAATTACAAAGCATAACTTTCAGATTCAAGATACTAGCGAAA
TTCCTAAGATATTTAGGAAGGCTTTTGAAATTGCAAAAACTGGGCGACCAGGTGCGGTTCATATCGACTTACCAAAAGAT
GTACAGGATAGTGAGTTGGATTTAGAAAAATACCCTATTCCTGCAGACATAAATTTACAAGGATATAAACCAACAACTTT
TGGACATCCTTTGCAGATTAAAAAAGCAGTTGAAATAATTCAGAATTCTAAAAGGCCAGTAATTATTGCTGGAGGGGGAG
TTCAAATTGCTAATGCAAGCTTAGAACTTATAAAATTATCCGAATTTGTAAAAATTCCCGTATGTACGACATTAATGGGT
AAAGGGGCGTTTCCTGAAGAACATCCTTTAGCGCTTGGATTAGTTGGAATGCATGGGACAAAAGCATCAAACTATTCAGT
CTATGAAAGTGACGTTTTAATTGCAATTGGCTGTAGATTTTCAGACCGGATTACTGGAGACTTATCCTCTTTTGCACCCC
ATGCAAAAATAATACATATAGATATAGACCCTGCAGAAATTGGAAAAAACGTAAGAGTGGATATCCCAATCGTAGGGGAT
GCAAAAGCAATTTTAAAAGATATTTTAACCCATATATTGAAAAAACAGCCAATAAATAAAAATGAATGGATGGAAAACGT
TAAAAAACTTCAAAAAGAATCCCTTCCAATCATGGACTTTGATAATAAGCCCGTAAAACCGCAAAGACTCATTAAAGAAA
TGATGCTTGCATTAAGGGAATTAGACCCTTCATTAAAAAATACGATTATTACAACAGATGTTGGGCAGAACCAGATGTGG
ATGGCACATTACTTCCAGTCAAGTGCTCCAAAATCGTTTATATCGTCAGGTGGGCAGGGTACAATGGGTTTTGGTTTTCC
TTCAGCAATTGGTGCAAAAATAGCTAGGCCTGATGCAAGTGTTATTTCAGTTACCGGAGATGGTGGATTTTTAATGAATT
CACAAGAACTTGCAACTATTTCAGAATACCAAATCCCGATTGTTATAGCGATATTTGATAACAGAACCCTTGGAATGGTT
TATCAGTGGCAAAATCTATACTATGGGAAAAGACAGTGTGCAGTTCATTTAGGAAATACTCCTGACTTTGTAAAACTTGC
AGAAAGCTATGGTGTTGATGCATTAAGAATCGAAAGTCCTGAAGATATTAAAGAATCATTTAAAACTGCCTTAAAAAGCG
GAAAACCTTATTTATTAGATATTATTATTGATCCAACAGAAGCTCTCCACATGGTGCCACCAGGAGGAACCATGACAAAC
ATATTATTCCCTGAAAGACAGGAACCTACTCCAAAGCCCACTTGCTTTAATGACATGAAAAAAATTCTTAATTTAAAGGT
CTAA

Upstream 100 bases:

>100_bases
TTTTTAAAGTCATAGTTTAATAATATTTATATAATATTTAGATTTAATAAAATAAAAAGCATTTAATTTTATAATCATGA
CATGTTAATTTGGTGATCCC

Downstream 100 bases:

>100_bases
TCAGGTGATTTCCAATGGAACACAAACATATTATTTCGGTCTTAGTACTACATAGGCCAGGAGTACTCCAAAGAATTTCA
GGATTATTTACAAGAAGATG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKGAEAMMKALKAENVKVLFGYPGGQLLPFYDALYHTDFVHILTRHEQAAAHAADGYARASGDVGVCVATSGPGATNLVT
GVATAHADSSPVVALTGQVPTRLIGNDAFQEIDALGIFMQITKHNFQIQDTSEIPKIFRKAFEIAKTGRPGAVHIDLPKD
VQDSELDLEKYPIPADINLQGYKPTTFGHPLQIKKAVEIIQNSKRPVIIAGGGVQIANASLELIKLSEFVKIPVCTTLMG
KGAFPEEHPLALGLVGMHGTKASNYSVYESDVLIAIGCRFSDRITGDLSSFAPHAKIIHIDIDPAEIGKNVRVDIPIVGD
AKAILKDILTHILKKQPINKNEWMENVKKLQKESLPIMDFDNKPVKPQRLIKEMMLALRELDPSLKNTIITTDVGQNQMW
MAHYFQSSAPKSFISSGGQGTMGFGFPSAIGAKIARPDASVISVTGDGGFLMNSQELATISEYQIPIVIAIFDNRTLGMV
YQWQNLYYGKRQCAVHLGNTPDFVKLAESYGVDALRIESPEDIKESFKTALKSGKPYLLDIIIDPTEALHMVPPGGTMTN
ILFPERQEPTPKPTCFNDMKKILNLKV

Sequences:

>Translated_587_residues
MKGAEAMMKALKAENVKVLFGYPGGQLLPFYDALYHTDFVHILTRHEQAAAHAADGYARASGDVGVCVATSGPGATNLVT
GVATAHADSSPVVALTGQVPTRLIGNDAFQEIDALGIFMQITKHNFQIQDTSEIPKIFRKAFEIAKTGRPGAVHIDLPKD
VQDSELDLEKYPIPADINLQGYKPTTFGHPLQIKKAVEIIQNSKRPVIIAGGGVQIANASLELIKLSEFVKIPVCTTLMG
KGAFPEEHPLALGLVGMHGTKASNYSVYESDVLIAIGCRFSDRITGDLSSFAPHAKIIHIDIDPAEIGKNVRVDIPIVGD
AKAILKDILTHILKKQPINKNEWMENVKKLQKESLPIMDFDNKPVKPQRLIKEMMLALRELDPSLKNTIITTDVGQNQMW
MAHYFQSSAPKSFISSGGQGTMGFGFPSAIGAKIARPDASVISVTGDGGFLMNSQELATISEYQIPIVIAIFDNRTLGMV
YQWQNLYYGKRQCAVHLGNTPDFVKLAESYGVDALRIESPEDIKESFKTALKSGKPYLLDIIIDPTEALHMVPPGGTMTN
ILFPERQEPTPKPTCFNDMKKILNLKV
>Mature_587_residues
MKGAEAMMKALKAENVKVLFGYPGGQLLPFYDALYHTDFVHILTRHEQAAAHAADGYARASGDVGVCVATSGPGATNLVT
GVATAHADSSPVVALTGQVPTRLIGNDAFQEIDALGIFMQITKHNFQIQDTSEIPKIFRKAFEIAKTGRPGAVHIDLPKD
VQDSELDLEKYPIPADINLQGYKPTTFGHPLQIKKAVEIIQNSKRPVIIAGGGVQIANASLELIKLSEFVKIPVCTTLMG
KGAFPEEHPLALGLVGMHGTKASNYSVYESDVLIAIGCRFSDRITGDLSSFAPHAKIIHIDIDPAEIGKNVRVDIPIVGD
AKAILKDILTHILKKQPINKNEWMENVKKLQKESLPIMDFDNKPVKPQRLIKEMMLALRELDPSLKNTIITTDVGQNQMW
MAHYFQSSAPKSFISSGGQGTMGFGFPSAIGAKIARPDASVISVTGDGGFLMNSQELATISEYQIPIVIAIFDNRTLGMV
YQWQNLYYGKRQCAVHLGNTPDFVKLAESYGVDALRIESPEDIKESFKTALKSGKPYLLDIIIDPTEALHMVPPGGTMTN
ILFPERQEPTPKPTCFNDMKKILNLKV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=25.7495590828924, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=26.0504201680672, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI87081685, Length=577, Percent_Identity=42.1143847487002, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=40.7473309608541, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=602, Percent_Identity=32.7242524916944, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=26.9299820466786, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=23.2394366197183, Blast_Score=168, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=564, Percent_Identity=26.241134751773, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=570, Percent_Identity=26.3157894736842, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=597, Percent_Identity=24.9581239530988, Blast_Score=155, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=40.4593639575972, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321524, Length=550, Percent_Identity=25.8181818181818, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320816, Length=569, Percent_Identity=22.3198594024605, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=527, Percent_Identity=26.1859582542694, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323073, Length=525, Percent_Identity=24.3809523809524, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320588, Length=530, Percent_Identity=23.9622641509434, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=502, Percent_Identity=23.3067729083665, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922626, Length=495, Percent_Identity=24.4444444444444, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64124; Mature: 64124

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGAEAMMKALKAENVKVLFGYPGGQLLPFYDALYHTDFVHILTRHEQAAAHAADGYARA
CCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
SGDVGVCVATSGPGATNLVTGVATAHADSSPVVALTGQVPTRLIGNDAFQEIDALGIFMQ
CCCEEEEEEECCCCCHHHHHHHEECCCCCCCEEEEECCCCHHHHCCHHHHHHHHHHHEEE
ITKHNFQIQDTSEIPKIFRKAFEIAKTGRPGAVHIDLPKDVQDSELDLEKYPIPADINLQ
EECCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCEEC
GYKPTTFGHPLQIKKAVEIIQNSKRPVIIAGGGVQIANASLELIKLSEFVKIPVCTTLMG
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEEHHHHHCCCHHHHHHC
KGAFPEEHPLALGLVGMHGTKASNYSVYESDVLIAIGCRFSDRITGDLSSFAPHAKIIHI
CCCCCCCCCEEEEEEECCCCCCCCCEEEECCEEEEEECCCCCCCCCCHHHCCCCCEEEEE
DIDPAEIGKNVRVDIPIVGDAKAILKDILTHILKKQPINKNEWMENVKKLQKESLPIMDF
ECCHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEC
DNKPVKPQRLIKEMMLALRELDPSLKNTIITTDVGQNQMWMAHYFQSSAPKSFISSGGQG
CCCCCCHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCHHHHHHHCCCCHHHHHCCCCC
TMGFGFPSAIGAKIARPDASVISVTGDGGFLMNSQELATISEYQIPIVIAIFDNRTLGMV
CCCCCCHHHHCCEECCCCCCEEEEECCCCEEECCCHHHHHCCCCCEEEEEEECCCEEEEE
YQWQNLYYGKRQCAVHLGNTPDFVKLAESYGVDALRIESPEDIKESFKTALKSGKPYLLD
EEECHHCCCCEEEEEEECCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCEEEE
IIIDPTEALHMVPPGGTMTNILFPERQEPTPKPTCFNDMKKILNLKV
EEECCCHHEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKGAEAMMKALKAENVKVLFGYPGGQLLPFYDALYHTDFVHILTRHEQAAAHAADGYARA
CCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
SGDVGVCVATSGPGATNLVTGVATAHADSSPVVALTGQVPTRLIGNDAFQEIDALGIFMQ
CCCEEEEEEECCCCCHHHHHHHEECCCCCCCEEEEECCCCHHHHCCHHHHHHHHHHHEEE
ITKHNFQIQDTSEIPKIFRKAFEIAKTGRPGAVHIDLPKDVQDSELDLEKYPIPADINLQ
EECCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCEEC
GYKPTTFGHPLQIKKAVEIIQNSKRPVIIAGGGVQIANASLELIKLSEFVKIPVCTTLMG
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEEHHHHHCCCHHHHHHC
KGAFPEEHPLALGLVGMHGTKASNYSVYESDVLIAIGCRFSDRITGDLSSFAPHAKIIHI
CCCCCCCCCEEEEEEECCCCCCCCCEEEECCEEEEEECCCCCCCCCCHHHCCCCCEEEEE
DIDPAEIGKNVRVDIPIVGDAKAILKDILTHILKKQPINKNEWMENVKKLQKESLPIMDF
ECCHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEC
DNKPVKPQRLIKEMMLALRELDPSLKNTIITTDVGQNQMWMAHYFQSSAPKSFISSGGQG
CCCCCCHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCHHHHHHHCCCCHHHHHCCCCC
TMGFGFPSAIGAKIARPDASVISVTGDGGFLMNSQELATISEYQIPIVIAIFDNRTLGMV
CCCCCCHHHHCCEECCCCCCEEEEECCCCEEECCCHHHHHCCCCCEEEEEEECCCEEEEE
YQWQNLYYGKRQCAVHLGNTPDFVKLAESYGVDALRIESPEDIKESFKTALKSGKPYLLD
EEECHHCCCCEEEEEEECCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCEEEE
IIIDPTEALHMVPPGGTMTNILFPERQEPTPKPTCFNDMKKILNLKV
EEECCCHHEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]