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The map label for this gene is rfc [H]

Identifier: 150400153

GI number: 150400153

Start: 1444910

End: 1445857

Strand: Reverse

Name: rfc [H]

Synonym: Mevan_1412

Alternate gene names: 150400153

Gene position: 1445857-1444910 (Counterclockwise)

Preceding gene: 150400154

Following gene: 150400152

Centisome position: 84.06

GC content: 33.44

Gene sequence:

>948_bases
ATGCAAAAACCATGGGTTGAAAAGTACAGGCCTGAAAACCTTGACGAAGTTGTTGGGCATCAAGAAATAATAAAAAGACT
CAAAAATTATGTTGAAAAAAAATCAATGCCCCACTTATTATTTAGTGGATCCCCCGGTGTTGGAAAAACCACTGCTGCAT
TATGCCTTGCAAAAGACCTTTACGGAAATACTTGGAAAGAAAATTTCCTTGAATTAAACAGTTCTGATGAAAGGGGAATC
GATGTAATTAGGACGAAAGTAAAGGACTTTGCAAGGACAAAACCAATTGGAGATGCGCCATTTAAAGTAATATTTTTAGA
TGAAAGTGATGCATTAACATCAGATGCGCAAAATGCACTTCGTAGAACGATGGAAAAGTATTCTGATATATGTAGATTCG
TATTAAGCTGTAACTATCCAAGTAAAATTATTCCCCCAATACAGTCAAGATGTGCAATTTTTAGATTTTCACCGTTAAAA
ACCGAAGACCTTGTTAAAAATTTAAAGGAAATTTCTGAAAAAGAAAGTATAAATGTTGAAAAAAGTGGAATGGATGCAAT
AATCTATGTTTCAGAAGGGGACATGAGAAAAGCAATTAATGTATTACAAACCGGTGCTGCGGTTTCAAAAAATATAAATG
AAACAGTAATTTATAAAGTCGCATCAAAGGCAAGGCCTGATGAAATTAAAAAAATGACTGAACTTGCATTAAATGGAAAA
TTCGTTGAAGCACGTGAACAGCTTTATAAGCTTATGATTGACTGGGGAATGAGTGGAGAAGATATAATAATACAGATATT
TAGAGAAGTTCCAAATTTAGAAATTTCTGAAAAAGAAAAAGTGCATTTAGTTGAAGCAATCGGAGAATGTGATTTTAGAA
TTGTTGAAGGTGCAAATGAAAGGATACAGTTAAGTGCACTACTTGCAAAGATGGGAATTTTAGAGTGA

Upstream 100 bases:

>100_bases
TTAAATTTTTAACGGTGTTCTTTTTAAATAGACAGATATTAATAAAATCAAGCAGATATTTTAATTAAATTTTTACAAGT
TGAATTTTGCGAGGGAAATT

Downstream 100 bases:

>100_bases
AAATACTTTTTAAAAATTATTTTTTAAGTTATTTAAATTATTTAAAGTTCTTTTTAAATTTTTTGTTTAAAAGCTTTCAA
ATTTAGAATTATTTCTAGCA

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MQKPWVEKYRPENLDEVVGHQEIIKRLKNYVEKKSMPHLLFSGSPGVGKTTAALCLAKDLYGNTWKENFLELNSSDERGI
DVIRTKVKDFARTKPIGDAPFKVIFLDESDALTSDAQNALRRTMEKYSDICRFVLSCNYPSKIIPPIQSRCAIFRFSPLK
TEDLVKNLKEISEKESINVEKSGMDAIIYVSEGDMRKAINVLQTGAAVSKNINETVIYKVASKARPDEIKKMTELALNGK
FVEAREQLYKLMIDWGMSGEDIIIQIFREVPNLEISEKEKVHLVEAIGECDFRIVEGANERIQLSALLAKMGILE

Sequences:

>Translated_315_residues
MQKPWVEKYRPENLDEVVGHQEIIKRLKNYVEKKSMPHLLFSGSPGVGKTTAALCLAKDLYGNTWKENFLELNSSDERGI
DVIRTKVKDFARTKPIGDAPFKVIFLDESDALTSDAQNALRRTMEKYSDICRFVLSCNYPSKIIPPIQSRCAIFRFSPLK
TEDLVKNLKEISEKESINVEKSGMDAIIYVSEGDMRKAINVLQTGAAVSKNINETVIYKVASKARPDEIKKMTELALNGK
FVEAREQLYKLMIDWGMSGEDIIIQIFREVPNLEISEKEKVHLVEAIGECDFRIVEGANERIQLSALLAKMGILE
>Mature_315_residues
MQKPWVEKYRPENLDEVVGHQEIIKRLKNYVEKKSMPHLLFSGSPGVGKTTAALCLAKDLYGNTWKENFLELNSSDERGI
DVIRTKVKDFARTKPIGDAPFKVIFLDESDALTSDAQNALRRTMEKYSDICRFVLSCNYPSKIIPPIQSRCAIFRFSPLK
TEDLVKNLKEISEKESINVEKSGMDAIIYVSEGDMRKAINVLQTGAAVSKNINETVIYKVASKARPDEIKKMTELALNGK
FVEAREQLYKLMIDWGMSGEDIIIQIFREVPNLEISEKEKVHLVEAIGECDFRIVEGANERIQLSALLAKMGILE

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily [H]

Homologues:

Organism=Homo sapiens, GI6677723, Length=311, Percent_Identity=39.5498392282958, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI194306571, Length=309, Percent_Identity=39.1585760517799, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI194306567, Length=309, Percent_Identity=39.1585760517799, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI31563534, Length=311, Percent_Identity=41.8006430868167, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI4506491, Length=318, Percent_Identity=40.8805031446541, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI31881687, Length=318, Percent_Identity=40.8805031446541, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI28882049, Length=310, Percent_Identity=36.7741935483871, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI194306569, Length=244, Percent_Identity=39.344262295082, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4506489, Length=224, Percent_Identity=36.6071428571429, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI108773789, Length=224, Percent_Identity=36.6071428571429, Blast_Score=138, Evalue=6e-33,
Organism=Escherichia coli, GI1786676, Length=217, Percent_Identity=31.7972350230415, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17541988, Length=313, Percent_Identity=41.5335463258786, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71986063, Length=311, Percent_Identity=40.5144694533762, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17554730, Length=214, Percent_Identity=41.588785046729, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17541990, Length=290, Percent_Identity=35.8620689655172, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324478, Length=315, Percent_Identity=42.8571428571429, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6324039, Length=314, Percent_Identity=39.4904458598726, Blast_Score=246, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6322528, Length=331, Percent_Identity=38.9728096676737, Blast_Score=228, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6319562, Length=292, Percent_Identity=29.4520547945205, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324791, Length=237, Percent_Identity=26.1603375527426, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17647857, Length=313, Percent_Identity=41.5335463258786, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19921076, Length=312, Percent_Identity=39.7435897435897, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI18859927, Length=318, Percent_Identity=37.4213836477987, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI19921136, Length=352, Percent_Identity=29.8295454545455, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748 [H]

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 35708; Mature: 35708

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPWVEKYRPENLDEVVGHQEIIKRLKNYVEKKSMPHLLFSGSPGVGKTTAALCLAKDL
CCCCCCHHCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHH
YGNTWKENFLELNSSDERGIDVIRTKVKDFARTKPIGDAPFKVIFLDESDALTSDAQNAL
HCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
RRTMEKYSDICRFVLSCNYPSKIIPPIQSRCAIFRFSPLKTEDLVKNLKEISEKESINVE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
KSGMDAIIYVSEGDMRKAINVLQTGAAVSKNINETVIYKVASKARPDEIKKMTELALNGK
CCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
FVEAREQLYKLMIDWGMSGEDIIIQIFREVPNLEISEKEKVHLVEAIGECDFRIVEGANE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCC
RIQLSALLAKMGILE
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKPWVEKYRPENLDEVVGHQEIIKRLKNYVEKKSMPHLLFSGSPGVGKTTAALCLAKDL
CCCCCCHHCCCCCHHHHHCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHH
YGNTWKENFLELNSSDERGIDVIRTKVKDFARTKPIGDAPFKVIFLDESDALTSDAQNAL
HCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
RRTMEKYSDICRFVLSCNYPSKIIPPIQSRCAIFRFSPLKTEDLVKNLKEISEKESINVE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCC
KSGMDAIIYVSEGDMRKAINVLQTGAAVSKNINETVIYKVASKARPDEIKKMTELALNGK
CCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
FVEAREQLYKLMIDWGMSGEDIIIQIFREVPNLEISEKEKVHLVEAIGECDFRIVEGANE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCC
RIQLSALLAKMGILE
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA