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The map label for this gene is purD

Identifier: 150400121

GI number: 150400121

Start: 1409371

End: 1410702

Strand: Reverse

Name: purD

Synonym: Mevan_1379

Alternate gene names: 150400121

Gene position: 1410702-1409371 (Counterclockwise)

Preceding gene: 150400122

Following gene: 150400120

Centisome position: 82.02

GC content: 33.48

Gene sequence:

>1332_bases
ATGAAAGTTTTACTTATTGGCGGTGGAGCACGGGAACACGCTATTGCAATGGCTTTAAAAAAAAATAGAAGTGTAGAACT
ATATACATTAATGAAAAATAAAAATCCGGGAATTTATGGATTGTCAAAAGAAGTATCATTTAACAATGAAACAGATATTG
ATAAAATAAAGGAATTTGCAGAAAAAATAAGGCCTGAACTTGCAATAATCGGCCCTGAAGCACCATTAGGAGTTGGCGCT
GCTGATTTATTAACGGAAATGGGGATTCCAACAGTCGGTCCAAAAAAACTTCCTGCACAGATTGAAACCAGTAAAGAGTT
CATGAGAAATTTATTTAAAAAATATAATATTAAAGGGTCACTAAAATACGCAGCTTTCAACGAATATGGAGAAAATCTAG
AAAAATTCATTGATGAAATGACTGGTTTAGGAAAAGACGTTGTTGTAAAACCAGCAGGATTAACGGGTGGCAAAGGCGTA
AAAGTTGTTGGTGAGCAGTTAAAAAATAATGACGATGCAAAGCTCTATGCAAAAGAAGTTTTTGAAAAAAGTATTGGTGG
CGGAAAAATCATTGTCGAAGAAAAACTTGTTGGAGTTGAATTTACGCTCCACGGATTTGTTGATGGTGAAAATATAGTAT
TTATGCCCGCAGTTCAAGACCATCCTCATGCATACAATAACGATGAAGGGCCAATAACTGGTGGAATGGGTTCTTATTCA
TGTTCTAACCATAAATTGCCATTTTTACCAGATGATAAATTAGATGAAGCTAAAGAGATAATGAAAGAAACAGTAAATGC
AATTAAGGCAGAAGTTGGACCATATAATGGGTTTTTATACGGCCAATTCATGCTTACTAAAGACGGGCCTAAAATTATAG
AATATAATGCAAGATTTGGAGACCCTGAGGCTATGAATTTACTTCCGATATTAAAAACAGACTTTTTAGACGTTTGTTTT
GGGATATCTAATGGAAATCTTGATAATATTAACATAGAATTTGAAAATAAAGCAACTGTTTGTAAATACGTGGTTCCAAA
TGGCTACCCGATATGCCCTGTAAAAAATAAAGAAATTTTGGTAAACAGTAACGAAATTGAAAAGGCAGGTGCAATTTTGT
TTTATGCATCAGTTAATGAAGAAAATGGAAAATTAGTCATAACTGGCTCTAGAAGTGCAGCGGTTACTGGAATTTCTGAA
AGAATTGAGGATGCTGAAAAAATCGCTCAAGACGCAATAATAAACTTTGAAGGCGAAATATTCTTTAGAAGCGATATTGG
AACCAAAAAATTAATTGAAAAAAGAATTGAAAGAATGAAAGAATTAATTTAG

Upstream 100 bases:

>100_bases
ATTTGATAATATATCGTTATTTAAATAAATTTAACACGAAAAAATCGTATTTAAAAAATTTTTAAAGATGGAATTTAAAA
AGGTTTACTTTGGTGATAGT

Downstream 100 bases:

>100_bases
AATTAAAAACTCCTGTAAGAACAATGTATATATAATAGAAATATATATCAAATTCCGTCGATATAATTGATTATGATTAA
AACATTATTATAGAGGGATT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKVLLIGGGAREHAIAMALKKNRSVELYTLMKNKNPGIYGLSKEVSFNNETDIDKIKEFAEKIRPELAIIGPEAPLGVGA
ADLLTEMGIPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLKYAAFNEYGENLEKFIDEMTGLGKDVVVKPAGLTGGKGV
KVVGEQLKNNDDAKLYAKEVFEKSIGGGKIIVEEKLVGVEFTLHGFVDGENIVFMPAVQDHPHAYNNDEGPITGGMGSYS
CSNHKLPFLPDDKLDEAKEIMKETVNAIKAEVGPYNGFLYGQFMLTKDGPKIIEYNARFGDPEAMNLLPILKTDFLDVCF
GISNGNLDNINIEFENKATVCKYVVPNGYPICPVKNKEILVNSNEIEKAGAILFYASVNEENGKLVITGSRSAAVTGISE
RIEDAEKIAQDAIINFEGEIFFRSDIGTKKLIEKRIERMKELI

Sequences:

>Translated_443_residues
MKVLLIGGGAREHAIAMALKKNRSVELYTLMKNKNPGIYGLSKEVSFNNETDIDKIKEFAEKIRPELAIIGPEAPLGVGA
ADLLTEMGIPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLKYAAFNEYGENLEKFIDEMTGLGKDVVVKPAGLTGGKGV
KVVGEQLKNNDDAKLYAKEVFEKSIGGGKIIVEEKLVGVEFTLHGFVDGENIVFMPAVQDHPHAYNNDEGPITGGMGSYS
CSNHKLPFLPDDKLDEAKEIMKETVNAIKAEVGPYNGFLYGQFMLTKDGPKIIEYNARFGDPEAMNLLPILKTDFLDVCF
GISNGNLDNINIEFENKATVCKYVVPNGYPICPVKNKEILVNSNEIEKAGAILFYASVNEENGKLVITGSRSAAVTGISE
RIEDAEKIAQDAIINFEGEIFFRSDIGTKKLIEKRIERMKELI
>Mature_443_residues
MKVLLIGGGAREHAIAMALKKNRSVELYTLMKNKNPGIYGLSKEVSFNNETDIDKIKEFAEKIRPELAIIGPEAPLGVGA
ADLLTEMGIPTVGPKKLPAQIETSKEFMRNLFKKYNIKGSLKYAAFNEYGENLEKFIDEMTGLGKDVVVKPAGLTGGKGV
KVVGEQLKNNDDAKLYAKEVFEKSIGGGKIIVEEKLVGVEFTLHGFVDGENIVFMPAVQDHPHAYNNDEGPITGGMGSYS
CSNHKLPFLPDDKLDEAKEIMKETVNAIKAEVGPYNGFLYGQFMLTKDGPKIIEYNARFGDPEAMNLLPILKTDFLDVCF
GISNGNLDNINIEFENKATVCKYVVPNGYPICPVKNKEILVNSNEIEKAGAILFYASVNEENGKLVITGSRSAAVTGISE
RIEDAEKIAQDAIINFEGEIFFRSDIGTKKLIEKRIERMKELI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=441, Percent_Identity=34.0136054421769, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4503915, Length=441, Percent_Identity=34.0136054421769, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI209869995, Length=441, Percent_Identity=34.0136054421769, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI209869993, Length=441, Percent_Identity=34.0136054421769, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1790438, Length=441, Percent_Identity=32.6530612244898, Blast_Score=202, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17567511, Length=438, Percent_Identity=32.4200913242009, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321203, Length=451, Percent_Identity=27.7161862527716, Blast_Score=162, Evalue=7e-41,
Organism=Drosophila melanogaster, GI62471619, Length=447, Percent_Identity=31.5436241610738, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24582400, Length=447, Percent_Identity=31.5436241610738, Blast_Score=172, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_METVS (A6US04)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323888.1
- ProteinModelPortal:   A6US04
- STRING:   A6US04
- GeneID:   5324746
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1379
- eggNOG:   arNOG04186
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 48772; Mature: 48772

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLIGGGAREHAIAMALKKNRSVELYTLMKNKNPGIYGLSKEVSFNNETDIDKIKEFA
CEEEEEECCCCHHHHEEEHHCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHH
EKIRPELAIIGPEAPLGVGAADLLTEMGIPTVGPKKLPAQIETSKEFMRNLFKKYNIKGS
HHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
LKYAAFNEYGENLEKFIDEMTGLGKDVVVKPAGLTGGKGVKVVGEQLKNNDDAKLYAKEV
EEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHH
FEKSIGGGKIIVEEKLVGVEFTLHGFVDGENIVFMPAVQDHPHAYNNDEGPITGGMGSYS
HHHCCCCCEEEEEEEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCEECCCCCCC
CSNHKLPFLPDDKLDEAKEIMKETVNAIKAEVGPYNGFLYGQFMLTKDGPKIIEYNARFG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCC
DPEAMNLLPILKTDFLDVCFGISNGNLDNINIEFENKATVCKYVVPNGYPICPVKNKEIL
CCCHHEEHHHHHHHHHHHHEECCCCCEEEEEEEECCCCEEEEEECCCCEEECCCCCCEEE
VNSNEIEKAGAILFYASVNEENGKLVITGSRSAAVTGISERIEDAEKIAQDAIINFEGEI
EECCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHEEECCCCE
FFRSDIGTKKLIEKRIERMKELI
EEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVLLIGGGAREHAIAMALKKNRSVELYTLMKNKNPGIYGLSKEVSFNNETDIDKIKEFA
CEEEEEECCCCHHHHEEEHHCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHH
EKIRPELAIIGPEAPLGVGAADLLTEMGIPTVGPKKLPAQIETSKEFMRNLFKKYNIKGS
HHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
LKYAAFNEYGENLEKFIDEMTGLGKDVVVKPAGLTGGKGVKVVGEQLKNNDDAKLYAKEV
EEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHH
FEKSIGGGKIIVEEKLVGVEFTLHGFVDGENIVFMPAVQDHPHAYNNDEGPITGGMGSYS
HHHCCCCCEEEEEEEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCEECCCCCCC
CSNHKLPFLPDDKLDEAKEIMKETVNAIKAEVGPYNGFLYGQFMLTKDGPKIIEYNARFG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCC
DPEAMNLLPILKTDFLDVCFGISNGNLDNINIEFENKATVCKYVVPNGYPICPVKNKEIL
CCCHHEEHHHHHHHHHHHHEECCCCCEEEEEEEECCCCEEEEEECCCCEEECCCCCCEEE
VNSNEIEKAGAILFYASVNEENGKLVITGSRSAAVTGISERIEDAEKIAQDAIINFEGEI
EECCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHEEECCCCE
FFRSDIGTKKLIEKRIERMKELI
EEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA