Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 150399970

GI number: 150399970

Start: 1258082

End: 1259806

Strand: Reverse

Name: glyS

Synonym: Mevan_1227

Alternate gene names: 150399970

Gene position: 1259806-1258082 (Counterclockwise)

Preceding gene: 150399971

Following gene: 150399967

Centisome position: 73.24

GC content: 34.43

Gene sequence:

>1725_bases
ATGGATAAATACGATAAAATAATAGATTTAACTAAAAGAAGGGGTTTTTTATGGAACTCTTTTGAAATATATGGTGGCAT
CGCAGGATTTTTTGATTACGGGCCACTTGGTGCAATTTTAAAAAGTAGCGTGATAAACACGTGGAGAAAACATTATATCA
TTAACGAAGGGTTTTATGAAATTGATAGCCCCACAGTAAACCCTTACGAAGTTTTAAAGGCATCCGGCCACGTTGAAAAC
TTCACAGACCCGCTCGTTGAATGTAAGTCATGTTTAGAATCATTTAGGGCTGATCATATTATTGAGGAAAATATAGAAGT
TGATACAGAAGGTAAAACCTTAAACGAACTCCAAGAAATGATTGAAAAGAATAATATAAAATGTCCAAAATGCGGTGGGG
AATTTAAAGAAGTAAATACGTTTAATTTAATGTTTGCAACGTCAATTGGCCCAGGCGGAAAAAGAGCGGCATTTATGCGG
CCCGAAACTGCACAAGGCATATTTATCCAATTTAAAAGAATAAGCCAATTTTTTAGAAATAAACTTCCATATGGTGCTGT
TCAAATTGGTAAGGCATACAGAAACGAAATTTCGCCAAGACAGGGCGTGATAAGGTTAAGAGAGTTTACCCAAGCTGAAG
CAGAATTTTTTATTGATTCAAGGAAAAAAGAGAATTTTGAAAAATTTGAAAGCGTAAAAGACTTGATTTTACCATTGCTT
CCAGGTAAAAACCAAGAGAACGAATCCTTAAGTTCTGAAGAAAAAATCGTTAGAATGAGCTTAGGTGATGCAGTTAAAAA
TAAAATAATCGCTCACGAAGCAATTGCATACTACATTGCAGTTACTAAAAAGTTCTTGATGGACATTGGAATTGACGAAA
CAAAATTAAGGTTCAGGCAGCACCTTCCAAATGAAATGGCCCATTATGCAGCGGATTGCTGGGATGCAGAATTATATACT
GACAGATACGGCTGGATAGAGTGTGTAGGTATTGCTGACAGGACTGATTACGACTTAAGAGCACACATGAAAAACAGTGG
TGAAGATTTATCCGTATTTGTTGAATTTGATGAATCAAAGGAAATTGAAGTTTATGAAATTGAATTAAACTATAAATTAG
TTGGTAAGACGTTTAAAGGGGACGCTAAAATATTAGAAGAATCCTTAAAAGAACTTTCAAATGACAAAATGGAAGAATTA
ATTAAAACACTCAAAAATAATGAAAAGTATATTTTAAAAACGTGTAAAAGAGATTTTGAAGTGTTAAATGAGTACTTGAC
TGTTAAAAAGGTTAAAAAGAATGTTACGGGGGAAAAAATAGTTCCACATGTAATTGAACCTTCATACGGTATTGATAGAA
TTACTTACTGCGTAATTGAACATGCTTTTAAAGAGGAAGACGATAGAACCCTTCTTGCTTTTGTAAACACCGTTTCACCA
GTAAAGGTCGGTGTTTTCCCACTCGTTAATAAAGAAGGAATGGACGAAATTGCAGAAAATTTAAAGAACAAATTTAGAAA
AAACGGGATAATTGCAGAATATGACGACAGTGGTGCAATTGGACGGAGATACATGCGAATGGACGAAATTGGAACCCCTT
TCTGCGTAACGGTTGATGGGGAAACTTTAAAGGATAATACTGTAACCATTAGGGAAAGAGATTCAAGAGAACAGTTTAGA
ATTCCGTTAGACGGTGTTGTTGATTATATTAAAGAAAAACTCTAA

Upstream 100 bases:

>100_bases
AACTACCGCAGATTTAATAGCATCGTCCCTTTTAATATTTTTACTCGATAGAATAAGTAGCGAAAAAACGATAATTTATT
AAGTGTTATAAGGTGATTTA

Downstream 100 bases:

>100_bases
CTTATTATTATCTTTTTAAATTAAATTATTAGTATAAAAACATTTTAATTAAATGCCTTATTCTTTACTGAAGTGATTAA
ATCATAAATCGGCATAATAA

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MDKYDKIIDLTKRRGFLWNSFEIYGGIAGFFDYGPLGAILKSSVINTWRKHYIINEGFYEIDSPTVNPYEVLKASGHVEN
FTDPLVECKSCLESFRADHIIEENIEVDTEGKTLNELQEMIEKNNIKCPKCGGEFKEVNTFNLMFATSIGPGGKRAAFMR
PETAQGIFIQFKRISQFFRNKLPYGAVQIGKAYRNEISPRQGVIRLREFTQAEAEFFIDSRKKENFEKFESVKDLILPLL
PGKNQENESLSSEEKIVRMSLGDAVKNKIIAHEAIAYYIAVTKKFLMDIGIDETKLRFRQHLPNEMAHYAADCWDAELYT
DRYGWIECVGIADRTDYDLRAHMKNSGEDLSVFVEFDESKEIEVYEIELNYKLVGKTFKGDAKILEESLKELSNDKMEEL
IKTLKNNEKYILKTCKRDFEVLNEYLTVKKVKKNVTGEKIVPHVIEPSYGIDRITYCVIEHAFKEEDDRTLLAFVNTVSP
VKVGVFPLVNKEGMDEIAENLKNKFRKNGIIAEYDDSGAIGRRYMRMDEIGTPFCVTVDGETLKDNTVTIRERDSREQFR
IPLDGVVDYIKEKL

Sequences:

>Translated_574_residues
MDKYDKIIDLTKRRGFLWNSFEIYGGIAGFFDYGPLGAILKSSVINTWRKHYIINEGFYEIDSPTVNPYEVLKASGHVEN
FTDPLVECKSCLESFRADHIIEENIEVDTEGKTLNELQEMIEKNNIKCPKCGGEFKEVNTFNLMFATSIGPGGKRAAFMR
PETAQGIFIQFKRISQFFRNKLPYGAVQIGKAYRNEISPRQGVIRLREFTQAEAEFFIDSRKKENFEKFESVKDLILPLL
PGKNQENESLSSEEKIVRMSLGDAVKNKIIAHEAIAYYIAVTKKFLMDIGIDETKLRFRQHLPNEMAHYAADCWDAELYT
DRYGWIECVGIADRTDYDLRAHMKNSGEDLSVFVEFDESKEIEVYEIELNYKLVGKTFKGDAKILEESLKELSNDKMEEL
IKTLKNNEKYILKTCKRDFEVLNEYLTVKKVKKNVTGEKIVPHVIEPSYGIDRITYCVIEHAFKEEDDRTLLAFVNTVSP
VKVGVFPLVNKEGMDEIAENLKNKFRKNGIIAEYDDSGAIGRRYMRMDEIGTPFCVTVDGETLKDNTVTIRERDSREQFR
IPLDGVVDYIKEKL
>Mature_574_residues
MDKYDKIIDLTKRRGFLWNSFEIYGGIAGFFDYGPLGAILKSSVINTWRKHYIINEGFYEIDSPTVNPYEVLKASGHVEN
FTDPLVECKSCLESFRADHIIEENIEVDTEGKTLNELQEMIEKNNIKCPKCGGEFKEVNTFNLMFATSIGPGGKRAAFMR
PETAQGIFIQFKRISQFFRNKLPYGAVQIGKAYRNEISPRQGVIRLREFTQAEAEFFIDSRKKENFEKFESVKDLILPLL
PGKNQENESLSSEEKIVRMSLGDAVKNKIIAHEAIAYYIAVTKKFLMDIGIDETKLRFRQHLPNEMAHYAADCWDAELYT
DRYGWIECVGIADRTDYDLRAHMKNSGEDLSVFVEFDESKEIEVYEIELNYKLVGKTFKGDAKILEESLKELSNDKMEEL
IKTLKNNEKYILKTCKRDFEVLNEYLTVKKVKKNVTGEKIVPHVIEPSYGIDRITYCVIEHAFKEEDDRTLLAFVNTVSP
VKVGVFPLVNKEGMDEIAENLKNKFRKNGIIAEYDDSGAIGRRYMRMDEIGTPFCVTVDGETLKDNTVTIRERDSREQFR
IPLDGVVDYIKEKL

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=583, Percent_Identity=40.6518010291595, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17554996, Length=582, Percent_Identity=43.127147766323, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI32564541, Length=582, Percent_Identity=43.127147766323, Blast_Score=475, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319597, Length=620, Percent_Identity=40.8064516129032, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6325338, Length=598, Percent_Identity=39.7993311036789, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664462, Length=581, Percent_Identity=41.4802065404475, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21357965, Length=581, Percent_Identity=41.4802065404475, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_METVS (A6URK3)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323737.1
- ProteinModelPortal:   A6URK3
- SMR:   A6URK3
- STRING:   A6URK3
- GeneID:   5325856
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1227
- eggNOG:   arNOG04591
- HOGENOM:   HBG319978
- OMA:   AYRNEIS
- ProtClustDB:   PRK04173
- GO:   GO:0005737
- HAMAP:   MF_00253_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 66173; Mature: 66173

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 96-96 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKYDKIIDLTKRRGFLWNSFEIYGGIAGFFDYGPLGAILKSSVINTWRKHYIINEGFYE
CCHHHHHHHHHHHCCCEECCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCEE
IDSPTVNPYEVLKASGHVENFTDPLVECKSCLESFRADHIIEENIEVDTEGKTLNELQEM
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCHHHHHHHH
IEKNNIKCPKCGGEFKEVNTFNLMFATSIGPGGKRAAFMRPETAQGIFIQFKRISQFFRN
HHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
KLPYGAVQIGKAYRNEISPRQGVIRLREFTQAEAEFFIDSRKKENFEKFESVKDLILPLL
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGKNQENESLSSEEKIVRMSLGDAVKNKIIAHEAIAYYIAVTKKFLMDIGIDETKLRFRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
HLPNEMAHYAADCWDAELYTDRYGWIECVGIADRTDYDLRAHMKNSGEDLSVFVEFDESK
HCCHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCCEEEEEEECCCC
EIEVYEIELNYKLVGKTFKGDAKILEESLKELSNDKMEELIKTLKNNEKYILKTCKRDFE
CEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VLNEYLTVKKVKKNVTGEKIVPHVIEPSYGIDRITYCVIEHAFKEEDDRTLLAFVNTVSP
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCC
VKVGVFPLVNKEGMDEIAENLKNKFRKNGIIAEYDDSGAIGRRYMRMDEIGTPFCVTVDG
CEECEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECC
ETLKDNTVTIRERDSREQFRIPLDGVVDYIKEKL
CEECCCEEEEEECCCCHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDKYDKIIDLTKRRGFLWNSFEIYGGIAGFFDYGPLGAILKSSVINTWRKHYIINEGFYE
CCHHHHHHHHHHHCCCEECCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCEE
IDSPTVNPYEVLKASGHVENFTDPLVECKSCLESFRADHIIEENIEVDTEGKTLNELQEM
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCEECCCCCHHHHHHHH
IEKNNIKCPKCGGEFKEVNTFNLMFATSIGPGGKRAAFMRPETAQGIFIQFKRISQFFRN
HHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
KLPYGAVQIGKAYRNEISPRQGVIRLREFTQAEAEFFIDSRKKENFEKFESVKDLILPLL
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGKNQENESLSSEEKIVRMSLGDAVKNKIIAHEAIAYYIAVTKKFLMDIGIDETKLRFRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
HLPNEMAHYAADCWDAELYTDRYGWIECVGIADRTDYDLRAHMKNSGEDLSVFVEFDESK
HCCHHHHHHHHHCCCCHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCCEEEEEEECCCC
EIEVYEIELNYKLVGKTFKGDAKILEESLKELSNDKMEELIKTLKNNEKYILKTCKRDFE
CEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VLNEYLTVKKVKKNVTGEKIVPHVIEPSYGIDRITYCVIEHAFKEEDDRTLLAFVNTVSP
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCC
VKVGVFPLVNKEGMDEIAENLKNKFRKNGIIAEYDDSGAIGRRYMRMDEIGTPFCVTVDG
CEECEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEEEECC
ETLKDNTVTIRERDSREQFRIPLDGVVDYIKEKL
CEECCCEEEEEECCCCHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA