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The map label for this gene is thiC

Identifier: 150399932

GI number: 150399932

Start: 1204009

End: 1205289

Strand: Reverse

Name: thiC

Synonym: Mevan_1189

Alternate gene names: 150399932

Gene position: 1205289-1204009 (Counterclockwise)

Preceding gene: 150399940

Following gene: 150399929

Centisome position: 70.07

GC content: 37.63

Gene sequence:

>1281_bases
ATGACCCAGTTAACTGATGCAAAATCAGGAATAGTTACTGAAGAGATGAAGTTTGTAGCCGACGAGGAAAAAATTGACGT
TAATTACCTTAGAAAACTTATTGAAAAAGGTTACGTGGTAATTCCAAAAAATATAAACCGAAAAACGAAGCCCGTTGGAA
TTGGGGATAACTTACGAACAAAAGTTAATGTAAATTTAGGTACCTCACCTGATTTTATTGATATTTCTTGTGAACTTAAA
AAAGTTGAAATTTCAAATAAATACGGTGCTGACGCAATTATGGACTTAAGTACCGGTGGAAATCTTCCAGAAATTAGAAG
TCAAATTATGAAAAACACGAATCTTCCAATTGGGACTGTTCCAATCTATGAAGTTGGTGTTGATTCAAAAGAAAAGTACG
GTCGAGTCATAGATATGGATGAAGACCTGATTTTTAACGTTATTGAAAGGCAAGCCCAAGAAGGTGTTGATTTTATGACA
CTGCACTGCGGCATTACAAAACAGAGCGTTGACACATTGAATAAAGACCCTAGAAAAATGGGGGTGGTAAGTAGAGGAGG
TGCATTTTTAACTGCGTATATAATGTACCACAACAAGGAAAATCCCCTTTATAGAGATTTTGACTACCTACTTGAAATTT
TAAAAGAACATGACGTAACTTTAAGTCTTGGCGATGGAATGAGGCCCGGATGTTTGCAGGACAATACGGATAGAGCCCAA
ATACAGGAATTAATAACTCTAGGGGAGTTAGTAGATAGATGTAGAGAAAAAGGGGTTCAAGTTATGGTTGAAGGTCCTGG
ACACGTCCCTTACAATAATATTCAAGCGAACATGCAAATTCAAAAAACAATCTGTAAAAACGCACCATTCTACGTTTTAG
GCCCGATTGTAACTGATTTAGCGCCAGGATACGACCATATTACAGCTGCAATCGGTGGAACCCTTGCAGCGGTAAGTGGT
GCAAATTTTTTATGTTATGTAACTCCTGCTGAACACGTCAGACTCATGAAAGAAGAAGAAGTAAAAGAAGGATTAATTGC
ATCAAAAATTGCAGCCCAAGCTGCTGATGTTGCAAAGGGAAATCCTGTTGCATGGAACAAGGAAAGACAGATGGCAGATG
CAAGAATAAAACATGACTGGGAAAGGCAGTTTGAAATTGCACTTGATAGCGATAAACCAAAAAAAATGAGGGAAGAAATT
CCTTCAAAAGACGAAAAAGCATGTAGTGTATGTGGAGACTACTGTGCTCTTTTAATGGTTGAAGAACTTGGAAAACGATA
G

Upstream 100 bases:

>100_bases
CTCTAAATCCCACATAATACCACGTCAAATATTTATTTAAATAATCAATACATACTATATAGTTACTCCAATATTATTAA
TAAACTATAAAAGTGATATT

Downstream 100 bases:

>100_bases
GTTTAAAGATATTTAAGGTTTATTTGTTATTTAAAATAATAAAAAATATCATTCTAAAGCTTTTAAAATATCTTCAAAAG
GAATTTTTCCTTCTCTTATT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTQLTDAKSGIVTEEMKFVADEEKIDVNYLRKLIEKGYVVIPKNINRKTKPVGIGDNLRTKVNVNLGTSPDFIDISCELK
KVEISNKYGADAIMDLSTGGNLPEIRSQIMKNTNLPIGTVPIYEVGVDSKEKYGRVIDMDEDLIFNVIERQAQEGVDFMT
LHCGITKQSVDTLNKDPRKMGVVSRGGAFLTAYIMYHNKENPLYRDFDYLLEILKEHDVTLSLGDGMRPGCLQDNTDRAQ
IQELITLGELVDRCREKGVQVMVEGPGHVPYNNIQANMQIQKTICKNAPFYVLGPIVTDLAPGYDHITAAIGGTLAAVSG
ANFLCYVTPAEHVRLMKEEEVKEGLIASKIAAQAADVAKGNPVAWNKERQMADARIKHDWERQFEIALDSDKPKKMREEI
PSKDEKACSVCGDYCALLMVEELGKR

Sequences:

>Translated_426_residues
MTQLTDAKSGIVTEEMKFVADEEKIDVNYLRKLIEKGYVVIPKNINRKTKPVGIGDNLRTKVNVNLGTSPDFIDISCELK
KVEISNKYGADAIMDLSTGGNLPEIRSQIMKNTNLPIGTVPIYEVGVDSKEKYGRVIDMDEDLIFNVIERQAQEGVDFMT
LHCGITKQSVDTLNKDPRKMGVVSRGGAFLTAYIMYHNKENPLYRDFDYLLEILKEHDVTLSLGDGMRPGCLQDNTDRAQ
IQELITLGELVDRCREKGVQVMVEGPGHVPYNNIQANMQIQKTICKNAPFYVLGPIVTDLAPGYDHITAAIGGTLAAVSG
ANFLCYVTPAEHVRLMKEEEVKEGLIASKIAAQAADVAKGNPVAWNKERQMADARIKHDWERQFEIALDSDKPKKMREEI
PSKDEKACSVCGDYCALLMVEELGKR
>Mature_425_residues
TQLTDAKSGIVTEEMKFVADEEKIDVNYLRKLIEKGYVVIPKNINRKTKPVGIGDNLRTKVNVNLGTSPDFIDISCELKK
VEISNKYGADAIMDLSTGGNLPEIRSQIMKNTNLPIGTVPIYEVGVDSKEKYGRVIDMDEDLIFNVIERQAQEGVDFMTL
HCGITKQSVDTLNKDPRKMGVVSRGGAFLTAYIMYHNKENPLYRDFDYLLEILKEHDVTLSLGDGMRPGCLQDNTDRAQI
QELITLGELVDRCREKGVQVMVEGPGHVPYNNIQANMQIQKTICKNAPFYVLGPIVTDLAPGYDHITAAIGGTLAAVSGA
NFLCYVTPAEHVRLMKEEEVKEGLIASKIAAQAADVAKGNPVAWNKERQMADARIKHDWERQFEIALDSDKPKKMREEIP
SKDEKACSVCGDYCALLMVEELGKR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=457, Percent_Identity=41.1378555798687, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_METVS (A6URG5)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323699.1
- STRING:   A6URG5
- GeneID:   5325320
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1189
- eggNOG:   arNOG04546
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47474; Mature: 47343

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLTDAKSGIVTEEMKFVADEEKIDVNYLRKLIEKGYVVIPKNINRKTKPVGIGDNLRT
CCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEE
KVNVNLGTSPDFIDISCELKKVEISNKYGADAIMDLSTGGNLPEIRSQIMKNTNLPIGTV
EEEEECCCCCCEEEEEEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCC
PIYEVGVDSKEKYGRVIDMDEDLIFNVIERQAQEGVDFMTLHCGITKQSVDTLNKDPRKM
CEEEECCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHH
GVVSRGGAFLTAYIMYHNKENPLYRDFDYLLEILKEHDVTLSLGDGMRPGCLQDNTDRAQ
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHH
IQELITLGELVDRCREKGVQVMVEGPGHVPYNNIQANMQIQKTICKNAPFYVLGPIVTDL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECHHHHHHHHCCCCEEEECCHHHHC
APGYDHITAAIGGTLAAVSGANFLCYVTPAEHVRLMKEEEVKEGLIASKIAAQAADVAKG
CCCHHHHHHHHCCHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NPVAWNKERQMADARIKHDWERQFEIALDSDKPKKMREEIPSKDEKACSVCGDYCALLMV
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
EELGKR
HHHCCC
>Mature Secondary Structure 
TQLTDAKSGIVTEEMKFVADEEKIDVNYLRKLIEKGYVVIPKNINRKTKPVGIGDNLRT
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEE
KVNVNLGTSPDFIDISCELKKVEISNKYGADAIMDLSTGGNLPEIRSQIMKNTNLPIGTV
EEEEECCCCCCEEEEEEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCC
PIYEVGVDSKEKYGRVIDMDEDLIFNVIERQAQEGVDFMTLHCGITKQSVDTLNKDPRKM
CEEEECCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHH
GVVSRGGAFLTAYIMYHNKENPLYRDFDYLLEILKEHDVTLSLGDGMRPGCLQDNTDRAQ
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHH
IQELITLGELVDRCREKGVQVMVEGPGHVPYNNIQANMQIQKTICKNAPFYVLGPIVTDL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEECHHHHHHHHCCCCEEEECCHHHHC
APGYDHITAAIGGTLAAVSGANFLCYVTPAEHVRLMKEEEVKEGLIASKIAAQAADVAKG
CCCHHHHHHHHCCHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NPVAWNKERQMADARIKHDWERQFEIALDSDKPKKMREEIPSKDEKACSVCGDYCALLMV
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHH
EELGKR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA