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The map label for this gene is tfb [H]

Identifier: 150399786

GI number: 150399786

Start: 1059088

End: 1060107

Strand: Reverse

Name: tfb [H]

Synonym: Mevan_1038

Alternate gene names: 150399786

Gene position: 1060107-1059088 (Counterclockwise)

Preceding gene: 150399787

Following gene: 150399785

Centisome position: 61.63

GC content: 38.82

Gene sequence:

>1020_bases
ATGAAAGTTGAACCCATTACAAAAGAAGATACAAAAAAGTCCGAAAGAAAAATAAAGCTAGCTGTAGCAAAGCCTGAGGA
CTATTCTAATAAAAACGTAATTCTTGAAAAAGAGGAAGAACTAATCTGTCCTGTTTGCGGAAGTAAAAATATAATTAAGG
ATTATGAAAGGGCTGAAATCGTTTGTGAAATGTGCGGGTGCGTTTTACAGCAGAATTTATTCGATGTCGGCCCAGAATGG
AGAGCATTCGACCATGAGCAACGTGTCAAAAGAAGTAGGGTTGGAGCTCCAATGACATATACTATTCATGATAAAGGCCT
TTCAACAGTTATTGATTGGAGAAATAAAGATAGTTACGGTAAAGACATTTCTGCGGATAAAAGGGCCCAGTTATACCGTT
TAAGAAAGTGGCAAAGGAGAATTAGGGTTTCTGATGCATCAGAAAGGAACTTGGCGTTTGCACTTTCAGAACTCGATAGA
ATCGCATCAAAATTAGGGCTTCCAAGAAACGTTAGGGAAAATGCTGCAGTTCTTTATAGGGGTGCTGTAGAAAAGGGATT
AATTCGTGGAAGGAGTATTGAAGGTGTTGCCGCAGCTGCACTTTATGCGGCATGTAGAAGATGTAAAGTTCCAAGAACTC
TTGATGAAATTGCGGAAGTTTCAAGAGTCGATAGAAAAGAAATTGGGAGAACATATAGGTTTATTTCAAGAGAATTAAAC
ATAAGACTCGCCCCGACAAATCCTATAGATTATGTTCCAAGGTTTGCATCCGAATTAAAATTACCTGGAGAGGTTGAATC
AAAAGCAATTTCAATTCTTCAGAAAGCAGGGGAACGAGGATTAACTAGTGGACGGGGTCCAACAGGTGTTGCCGCAGCTG
CAATATATATTGCAAGCGTTTTACAAGGAACTAGGCGTACACAAAGAGAAGTTGCAGATGTTGCAGGAGTTACTGAAGTT
ACAATTAGAAATAGATACAAGGAACTTACTGAACACTTAGATATCGACGTTACACTTTAA

Upstream 100 bases:

>100_bases
AGACGCTATGGAATCAGTTAAAGTCAGTCACGTTTTCGTTTTAGAAGAGCAGCAGAAGACCCGTAAAAAAGGAAGGAAAT
AACTTTAACGCGGTGTTATC

Downstream 100 bases:

>100_bases
AAAAGGGTGAACTGTTATGGGACTGTTTGATAGGATTCAAAAATCCGATAAACCAGCTATTGCTAAAAAAGATTTAGCTG
AAAGTAATATAAACACCCCA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKVEPITKEDTKKSERKIKLAVAKPEDYSNKNVILEKEEELICPVCGSKNIIKDYERAEIVCEMCGCVLQQNLFDVGPEW
RAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDR
IASKLGLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEVSRVDRKEIGRTYRFISRELN
IRLAPTNPIDYVPRFASELKLPGEVESKAISILQKAGERGLTSGRGPTGVAAAAIYIASVLQGTRRTQREVADVAGVTEV
TIRNRYKELTEHLDIDVTL

Sequences:

>Translated_339_residues
MKVEPITKEDTKKSERKIKLAVAKPEDYSNKNVILEKEEELICPVCGSKNIIKDYERAEIVCEMCGCVLQQNLFDVGPEW
RAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDR
IASKLGLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEVSRVDRKEIGRTYRFISRELN
IRLAPTNPIDYVPRFASELKLPGEVESKAISILQKAGERGLTSGRGPTGVAAAAIYIASVLQGTRRTQREVADVAGVTEV
TIRNRYKELTEHLDIDVTL
>Mature_339_residues
MKVEPITKEDTKKSERKIKLAVAKPEDYSNKNVILEKEEELICPVCGSKNIIKDYERAEIVCEMCGCVLQQNLFDVGPEW
RAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDR
IASKLGLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEVSRVDRKEIGRTYRFISRELN
IRLAPTNPIDYVPRFASELKLPGEVESKAISILQKAGERGLTSGRGPTGVAAAAIYIASVLQGTRRTQREVADVAGVTEV
TIRNRYKELTEHLDIDVTL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=294, Percent_Identity=36.734693877551, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI22035556, Length=302, Percent_Identity=28.476821192053, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17564892, Length=288, Percent_Identity=36.1111111111111, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17531397, Length=292, Percent_Identity=25, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325343, Length=310, Percent_Identity=29.3548387096774, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321685, Length=186, Percent_Identity=28.494623655914, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17136758, Length=287, Percent_Identity=35.8885017421603, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI281361961, Length=302, Percent_Identity=27.4834437086093, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647658, Length=302, Percent_Identity=27.4834437086093, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 38257; Mature: 38257

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS51134 ZF_TFIIB ; PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEPITKEDTKKSERKIKLAVAKPEDYSNKNVILEKEEELICPVCGSKNIIKDYERAEI
CCCCCCCCHHHHHHHCEEEEEEECCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHH
VCEMCGCVLQQNLFDVGPEWRAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCC
KDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKLGLPRNVRENAAVLYR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEVSRVDRKEIGRTYRFISRELN
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCE
IRLAPTNPIDYVPRFASELKLPGEVESKAISILQKAGERGLTSGRGPTGVAAAAIYIASV
EEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LQGTRRTQREVADVAGVTEVTIRNRYKELTEHLDIDVTL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MKVEPITKEDTKKSERKIKLAVAKPEDYSNKNVILEKEEELICPVCGSKNIIKDYERAEI
CCCCCCCCHHHHHHHCEEEEEEECCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHH
VCEMCGCVLQQNLFDVGPEWRAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYG
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCC
KDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKLGLPRNVRENAAVLYR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEVSRVDRKEIGRTYRFISRELN
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCE
IRLAPTNPIDYVPRFASELKLPGEVESKAISILQKAGERGLTSGRGPTGVAAAAIYIASV
EEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LQGTRRTQREVADVAGVTEVTIRNRYKELTEHLDIDVTL
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA