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The map label for this gene is cysH [C]

Identifier: 150399729

GI number: 150399729

Start: 997697

End: 998983

Strand: Reverse

Name: cysH [C]

Synonym: Mevan_0981

Alternate gene names: 150399729

Gene position: 998983-997697 (Counterclockwise)

Preceding gene: 150399730

Following gene: 150399727

Centisome position: 58.08

GC content: 30.15

Gene sequence:

>1287_bases
ATGTTTGATGAAGACACGAAATTTGCATCTAATTATGAATTAAAAGTTTTAAATGAATTAACTGGCCAAAATTTTCAAAA
AGACGACGTAATTCTTTTAGAAAAACTTTCTGGATTAGACTATCGAAAAAGAGTATACATTGGAAATGATCAAATTGGAA
CACTTGAATTTGATTTACACGACCTTGAATGGAAATTTGTGCCATCTCCTTATTTTTACACGATAGCTGAACCAAAAATC
GCTTTGAAATACACGAAAAAAAGACTTAAAGGAAAATATATAAAAGAAGAACTATTGGAAAACCCTGAGGAGTACAATGC
GCTCTTAAAAAATGATTTTGAATATGTGGGCATAACAATCGGTGATTTTTTAGGAGTAGGCGTTAGAAAAGACGACCGTA
TAAAATTAAAAGACCTTTCAAGGAAAAAGGAACTTGAAATTGAAAAAATGCCTGAATTTTTAGAAAAAAATAAGGAAAGA
ATGAATGAAATGGTTGAAAATTCAATAAACATACTTGAAGAAACAATTGAAAAATATAAAAAGAAAGGATACGTAATAAA
CGTTTCATTTAGTGGCGGAAAAGATAGTTCAGTATGTACTTTACTTTCAAAAGAAGTAATGCCTGAAATGGACGTTGTAT
TTATTGATACTGGATTAGAATATCCTGAAACAATTCAATACGTTAAGGATTTTTCAAAAAAATACGATATTGAAATTGAC
ACCGTTGATGGAGACAATTTTTGGGATAATCTTGAAAAAGAGGGAATTCCAACAAAAGATAACCGATGGTGCAACAGTGC
CTGTAAATTAATGCCTTTAAAAAGGTATTTGAAAAAAAAGTATGGGAATAGAAAAGTTCTTACGATTGATGGTTCAAGAA
AATACGAAAGTTTTGCACGTGCAAACTTGGATTACGAACGAAAAAGTGGATTTATCGATTTTCAGACTAACGTATTTCCA
ATACTTGACTGGAACTCTTTAGATATTTGGACATACATTTATTCAAATGATATTTTATATAATCCGATGTACGATAAAGG
TTTTGAAAGAATAGGGTGTTATTTATGCCCGTCTGCATTAAACAGCGAATTTTTAAGGGTAGAAGAACTATATCCTGACC
ATTTTGAAAGATGGGTTAAATATCTGAAAAAATATTATCCAAAAGAAGAAGTTTTAAGAGGATTTTGGCGATGGAAAGAA
CTTCCTCCAAAAATGGTAGAACTTGAAGATGAATTAGGATATAATGGTGAAAGAAAGAAAAAAATAAAAAGAATTACTCA
ACTGTAA

Upstream 100 bases:

>100_bases
ATCTTAGATAAATCTTAGATAAATCTTAGATAAATCTTAGATAAATCTTAGATAAATCTTAGATAAATTTTAAATGGTAA
AAAAGAACAGGTAACCAAAA

Downstream 100 bases:

>100_bases
CACTTTTTGCAAGCCCTCTAGGCTTATCTACATCTCTTTTTAATTCAACAGCTTTATAGTATGCTAAAAGCTGACAAACA
GGTGCATAAATAAAAGGACT

Product: hypothetical protein

Products: H+; Adenosine 3',5'-bisphosphate; Sulfite; Oxidized thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MFDEDTKFASNYELKVLNELTGQNFQKDDVILLEKLSGLDYRKRVYIGNDQIGTLEFDLHDLEWKFVPSPYFYTIAEPKI
ALKYTKKRLKGKYIKEELLENPEEYNALLKNDFEYVGITIGDFLGVGVRKDDRIKLKDLSRKKELEIEKMPEFLEKNKER
MNEMVENSINILEETIEKYKKKGYVINVSFSGGKDSSVCTLLSKEVMPEMDVVFIDTGLEYPETIQYVKDFSKKYDIEID
TVDGDNFWDNLEKEGIPTKDNRWCNSACKLMPLKRYLKKKYGNRKVLTIDGSRKYESFARANLDYERKSGFIDFQTNVFP
ILDWNSLDIWTYIYSNDILYNPMYDKGFERIGCYLCPSALNSEFLRVEELYPDHFERWVKYLKKYYPKEEVLRGFWRWKE
LPPKMVELEDELGYNGERKKKIKRITQL

Sequences:

>Translated_428_residues
MFDEDTKFASNYELKVLNELTGQNFQKDDVILLEKLSGLDYRKRVYIGNDQIGTLEFDLHDLEWKFVPSPYFYTIAEPKI
ALKYTKKRLKGKYIKEELLENPEEYNALLKNDFEYVGITIGDFLGVGVRKDDRIKLKDLSRKKELEIEKMPEFLEKNKER
MNEMVENSINILEETIEKYKKKGYVINVSFSGGKDSSVCTLLSKEVMPEMDVVFIDTGLEYPETIQYVKDFSKKYDIEID
TVDGDNFWDNLEKEGIPTKDNRWCNSACKLMPLKRYLKKKYGNRKVLTIDGSRKYESFARANLDYERKSGFIDFQTNVFP
ILDWNSLDIWTYIYSNDILYNPMYDKGFERIGCYLCPSALNSEFLRVEELYPDHFERWVKYLKKYYPKEEVLRGFWRWKE
LPPKMVELEDELGYNGERKKKIKRITQL
>Mature_428_residues
MFDEDTKFASNYELKVLNELTGQNFQKDDVILLEKLSGLDYRKRVYIGNDQIGTLEFDLHDLEWKFVPSPYFYTIAEPKI
ALKYTKKRLKGKYIKEELLENPEEYNALLKNDFEYVGITIGDFLGVGVRKDDRIKLKDLSRKKELEIEKMPEFLEKNKER
MNEMVENSINILEETIEKYKKKGYVINVSFSGGKDSSVCTLLSKEVMPEMDVVFIDTGLEYPETIQYVKDFSKKYDIEID
TVDGDNFWDNLEKEGIPTKDNRWCNSACKLMPLKRYLKKKYGNRKVLTIDGSRKYESFARANLDYERKSGFIDFQTNVFP
ILDWNSLDIWTYIYSNDILYNPMYDKGFERIGCYLCPSALNSEFLRVEELYPDHFERWVKYLKKYYPKEEVLRGFWRWKE
LPPKMVELEDELGYNGERKKKIKRITQL

Specific function: Reduction Of Activated Sulfate Into Sulfite. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PAPS reductase family [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=179, Percent_Identity=25.1396648044693, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: 1.8.4.8 [C]

Molecular weight: Translated: 50963; Mature: 50963

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDEDTKFASNYELKVLNELTGQNFQKDDVILLEKLSGLDYRKRVYIGNDQIGTLEFDLH
CCCCCCCCCCCCCEEEHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEEEE
DLEWKFVPSPYFYTIAEPKIALKYTKKRLKGKYIKEELLENPEEYNALLKNDFEYVGITI
CCEEEECCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHCCCEEEEEEH
GDFLGVGVRKDDRIKLKDLSRKKELEIEKMPEFLEKNKERMNEMVENSINILEETIEKYK
HHHHCCCCCCCCCEEHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGYVINVSFSGGKDSSVCTLLSKEVMPEMDVVFIDTGLEYPETIQYVKDFSKKYDIEID
HCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEE
TVDGDNFWDNLEKEGIPTKDNRWCNSACKLMPLKRYLKKKYGNRKVLTIDGSRKYESFAR
ECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
ANLDYERKSGFIDFQTNVFPILDWNSLDIWTYIYSNDILYNPMYDKGFERIGCYLCPSAL
CCCCHHHHCCEEEEECCEEEEEECCCCEEEEEEECCCEEECCCHHHHHHHHHHEECCHHH
NSEFLRVEELYPDHFERWVKYLKKYYPKEEVLRGFWRWKELPPKMVELEDELGYNGERKK
CHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCHHCCCCCHHHH
KIKRITQL
HHHHHHCC
>Mature Secondary Structure
MFDEDTKFASNYELKVLNELTGQNFQKDDVILLEKLSGLDYRKRVYIGNDQIGTLEFDLH
CCCCCCCCCCCCCEEEHHHHCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEEEE
DLEWKFVPSPYFYTIAEPKIALKYTKKRLKGKYIKEELLENPEEYNALLKNDFEYVGITI
CCEEEECCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHCCCEEEEEEH
GDFLGVGVRKDDRIKLKDLSRKKELEIEKMPEFLEKNKERMNEMVENSINILEETIEKYK
HHHHCCCCCCCCCEEHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGYVINVSFSGGKDSSVCTLLSKEVMPEMDVVFIDTGLEYPETIQYVKDFSKKYDIEID
HCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEE
TVDGDNFWDNLEKEGIPTKDNRWCNSACKLMPLKRYLKKKYGNRKVLTIDGSRKYESFAR
ECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
ANLDYERKSGFIDFQTNVFPILDWNSLDIWTYIYSNDILYNPMYDKGFERIGCYLCPSAL
CCCCHHHHCCEEEEECCEEEEEECCCCEEEEEEECCCEEECCCHHHHHHHHHHEECCHHH
NSEFLRVEELYPDHFERWVKYLKKYYPKEEVLRGFWRWKELPPKMVELEDELGYNGERKK
CHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCHHCCCCCHHHH
KIKRITQL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 5700 [C]

Specific activity: 99

Km value (mM): 0.01 {5-phosphoadenosine} [C]

Substrates: 3'-Phosphoadenylyl sulfate; Reduced thioredoxin [C]

Specific reaction: 3'-Phosphoadenylyl sulfate + Reduced thioredoxin --> (2) H+ + Adenosine 3',5'-bisphosphate + Sulfite + Oxidized thioredoxin [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: Adenosine3', 5'-bisphosphate; N-Bromosuccinimide; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]