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The map label for this gene is pdxT

Identifier: 150399704

GI number: 150399704

Start: 974248

End: 974811

Strand: Reverse

Name: pdxT

Synonym: Mevan_0956

Alternate gene names: 150399704

Gene position: 974811-974248 (Counterclockwise)

Preceding gene: 150399706

Following gene: 150399702

Centisome position: 56.67

GC content: 28.55

Gene sequence:

>564_bases
ATGAAAATTATTGGAATACTTGGAATTCAAGGAGATATTGAAGAACATGAAATAGCTGTTAAAAAAATAAACTGCATTCC
AAAGAGAATACGGTCAGTAAGTGATTTAGATGAAATTGATGCTTTAATTATTCCGGGTGGGGAAAGCACTACAATTGGAA
AATTAATGGTAAAATATGGATTTATCGAGAAAATTAGAAATTTAGATATTCCAATACTTGGAACCTGTGCAGGAATGGTT
CTTTTATCAAAGGGAACCGGAAAAGAACAACCTCTTTTAAAAATATTAAACGTAACAATTAAAAGGAATGCATATGGTAG
TCAAAAAGATAGCTTTGAAAAGGAAATAATTTTAGATGGTAAAAAACTAAATGCAGTATTTATAAGGGCACCAATGGTCG
ATAAAATTTTAGAAAAAACAGTTGAAATTATTTCAAAAGATGGTGAAAGTATTGTTGGAGTACGGGAAGGAAATATAATG
GCAATATCATTTCACCCTGAACTTTCAAATGATGGTATTATACTTTATGAATATTTTTTAAAAAATTTCGTTGAAAAAAA
TTAA

Upstream 100 bases:

>100_bases
TTTTAGCATATTTAATTTAGCATATTTAATTTAGCATATTTAATTTAGCATATTTAATTTAGCATATTTAATTTAGCATA
TTTAAAAAACGGTGAAAAAC

Downstream 100 bases:

>100_bases
AATAATTCTATTTATTTTCCTTTCCGTTTTTCAAATAATTCATTTAATTCGGAACTAACATACGGATTTGAACTATCTTT
ATACATCATAAGCTTTTCAA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKIIGILGIQGDIEEHEIAVKKINCIPKRIRSVSDLDEIDALIIPGGESTTIGKLMVKYGFIEKIRNLDIPILGTCAGMV
LLSKGTGKEQPLLKILNVTIKRNAYGSQKDSFEKEIILDGKKLNAVFIRAPMVDKILEKTVEIISKDGESIVGVREGNIM
AISFHPELSNDGIILYEYFLKNFVEKN

Sequences:

>Translated_187_residues
MKIIGILGIQGDIEEHEIAVKKINCIPKRIRSVSDLDEIDALIIPGGESTTIGKLMVKYGFIEKIRNLDIPILGTCAGMV
LLSKGTGKEQPLLKILNVTIKRNAYGSQKDSFEKEIILDGKKLNAVFIRAPMVDKILEKTVEIISKDGESIVGVREGNIM
AISFHPELSNDGIILYEYFLKNFVEKN
>Mature_187_residues
MKIIGILGIQGDIEEHEIAVKKINCIPKRIRSVSDLDEIDALIIPGGESTTIGKLMVKYGFIEKIRNLDIPILGTCAGMV
LLSKGTGKEQPLLKILNVTIKRNAYGSQKDSFEKEIILDGKKLNAVFIRAPMVDKILEKTVEIISKDGESIVGVREGNIM
AISFHPELSNDGIILYEYFLKNFVEKN

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=213, Percent_Identity=32.8638497652582, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323995, Length=218, Percent_Identity=34.8623853211009, Blast_Score=112, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321048, Length=218, Percent_Identity=34.4036697247706, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_METVS (A6UQT7)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323471.1
- ProteinModelPortal:   A6UQT7
- SMR:   A6UQT7
- STRING:   A6UQT7
- GeneID:   5325955
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0956
- eggNOG:   arNOG08412
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13527
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20756; Mature: 20756

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 76-76 ACT_SITE 165-165 ACT_SITE 167-167 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIGILGIQGDIEEHEIAVKKINCIPKRIRSVSDLDEIDALIIPGGESTTIGKLMVKYG
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHHHHH
FIEKIRNLDIPILGTCAGMVLLSKGTGKEQPLLKILNVTIKRNAYGSQKDSFEKEIILDG
HHHHHHCCCCCEEHHHHCEEEEECCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCEEECC
KKLNAVFIRAPMVDKILEKTVEIISKDGESIVGVREGNIMAISFHPELSNDGIILYEYFL
CEEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCEEEHHHHH
KNFVEKN
HHHHCCC
>Mature Secondary Structure
MKIIGILGIQGDIEEHEIAVKKINCIPKRIRSVSDLDEIDALIIPGGESTTIGKLMVKYG
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHHHHH
FIEKIRNLDIPILGTCAGMVLLSKGTGKEQPLLKILNVTIKRNAYGSQKDSFEKEIILDG
HHHHHHCCCCCEEHHHHCEEEEECCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCEEECC
KKLNAVFIRAPMVDKILEKTVEIISKDGESIVGVREGNIMAISFHPELSNDGIILYEYFL
CEEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCEEEHHHHH
KNFVEKN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA