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The map label for this gene is hyfB [C]

Identifier: 150399686

GI number: 150399686

Start: 953019

End: 954467

Strand: Reverse

Name: hyfB [C]

Synonym: Mevan_0937

Alternate gene names: 150399686

Gene position: 954467-953019 (Counterclockwise)

Preceding gene: 150399687

Following gene: 150399685

Centisome position: 55.49

GC content: 34.71

Gene sequence:

>1449_bases
ATGAACTTACTTCCTTTGATAGTAGTTTTTCCAATGTTCGTGGCCATCATATTCAATTATTTAAATGGGAAGGATAAATT
AATTCGACCTATGGCATTTTTAATGGCCCTTTTACTACTTGTACTTCCATTTATTGGAAACTATGGAATTTATAATTTTG
GAGCCCATGGCTTAGAAAGTGGACTTGCTTCAGGTATTTCTTACATATATAGCCCAATGAAACAGCTTATAATCACCGTT
TTAATGTTAATCGGCTCATTAGTTTTGATTACGGGGCTTGATGAAAAAAAATCGAGTGGATTAACAGTTGCATTAATGTT
AATGGGTTTAGCAAGTGTTTCAGCAGTTATTTTAGCAGATGATTTATTCAATATATACGTATTCTATGAAATAGCGGCAA
TTGCCCAAACGGGATTAGTTATTGCTTCTGGAACTGAAAAAGCTTATCGTTCAGCATTTAGATACTTACTTTTAGGAAAT
TTTGCAGGTTCAATTTTACTACTTGGCGTAGCTTTTTTACTTTCTGCAACAGGAACGTTAAATATTTCAGACATGCACAA
TTTTTTAATAAATAATCCGGTAACTCCAACAATTTATGGCGGACTTTTAATGATCATAATAGGTTTATGTTATGATAGTG
GCCTTCCTCCTTTTCACACTGTAAAAGCGGACATTTATGCACGTGCAAAACCATTTATAGCCGCAATGCTCCAAACATAC
TCAAAATTTGTTCTTGTAGCATTAATGCTTGTAATTTTTAAAATATTTGGTGGACTTTCATATTTTACAAATGCACACGG
GGCTTTAATTGCAATATCAATTCTTGGAATGGTATTTGGTGTAGTAATGGCTCTTTTACAGACAGATTATAGAAAATTGC
TTGCATACCACGCAATAAGTCAGGGAGGTTATGTTGCAGCAGGTATTGCACTTGGAACACCTTTAGGAATTGTTGCAGGA
ATTTTTCACGCAATAAATCACGTTATATATAAGAGTGCTCTTTTCATTGGAGCCCACCTTGTGGAAAGAAAAAAGGTTGG
AAATTTAAATGCACTTGGTGGTCTTTTACCCGTAATTCCCACAACTGCACTGATGGTTTTATGTGCAAAACTCGCAATTA
GTGGCGTTCCGCCATTTAATGGATTTCAGAGTAAATTACTACTGGCAGAAGCTGCAATGAATGTGAATATGCCCGAACTT
GCAATTATAATGATAATGGTAAGTATTGGGACTTTTGTATCCATGATGAAAGCGTTTTATTTGATATATCTAAAACCCTG
CAGTGAAGATCAGCTAAATGAATATAAAAAAGTGAAACCTTCGAAATTTGGGATATTTTCACTTGCGGTTTTAACAGTTC
TCTGTATTTTATTAGGCGTATACCCAAATCTTGCAACGGATTTAATATACCCCTTCGCAAACGAATTTGGAAGAATGTGG
ACGTTATAA

Upstream 100 bases:

>100_bases
TGCTTGGACTTTCAATAGTTTTTTACAAAAAATACAAAACACTAAAGTCATCAGAACTTTTAAAAGACGATTAACGATTT
AACTTTAAACGGTGAGCTTA

Downstream 100 bases:

>100_bases
TTAATTCGGTGATAAAATGTCTTACAAGGACTTTAAAAAAGAAATGGATAAACAAAATCACGGGGACGGTATTTCACTTG
GAGCAGTTTTAACCGCAGAA

Product: hydrogenase subunit F

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNLLPLIVVFPMFVAIIFNYLNGKDKLIRPMAFLMALLLLVLPFIGNYGIYNFGAHGLESGLASGISYIYSPMKQLIITV
LMLIGSLVLITGLDEKKSSGLTVALMLMGLASVSAVILADDLFNIYVFYEIAAIAQTGLVIASGTEKAYRSAFRYLLLGN
FAGSILLLGVAFLLSATGTLNISDMHNFLINNPVTPTIYGGLLMIIIGLCYDSGLPPFHTVKADIYARAKPFIAAMLQTY
SKFVLVALMLVIFKIFGGLSYFTNAHGALIAISILGMVFGVVMALLQTDYRKLLAYHAISQGGYVAAGIALGTPLGIVAG
IFHAINHVIYKSALFIGAHLVERKKVGNLNALGGLLPVIPTTALMVLCAKLAISGVPPFNGFQSKLLLAEAAMNVNMPEL
AIIMIMVSIGTFVSMMKAFYLIYLKPCSEDQLNEYKKVKPSKFGIFSLAVLTVLCILLGVYPNLATDLIYPFANEFGRMW
TL

Sequences:

>Translated_482_residues
MNLLPLIVVFPMFVAIIFNYLNGKDKLIRPMAFLMALLLLVLPFIGNYGIYNFGAHGLESGLASGISYIYSPMKQLIITV
LMLIGSLVLITGLDEKKSSGLTVALMLMGLASVSAVILADDLFNIYVFYEIAAIAQTGLVIASGTEKAYRSAFRYLLLGN
FAGSILLLGVAFLLSATGTLNISDMHNFLINNPVTPTIYGGLLMIIIGLCYDSGLPPFHTVKADIYARAKPFIAAMLQTY
SKFVLVALMLVIFKIFGGLSYFTNAHGALIAISILGMVFGVVMALLQTDYRKLLAYHAISQGGYVAAGIALGTPLGIVAG
IFHAINHVIYKSALFIGAHLVERKKVGNLNALGGLLPVIPTTALMVLCAKLAISGVPPFNGFQSKLLLAEAAMNVNMPEL
AIIMIMVSIGTFVSMMKAFYLIYLKPCSEDQLNEYKKVKPSKFGIFSLAVLTVLCILLGVYPNLATDLIYPFANEFGRMW
TL
>Mature_482_residues
MNLLPLIVVFPMFVAIIFNYLNGKDKLIRPMAFLMALLLLVLPFIGNYGIYNFGAHGLESGLASGISYIYSPMKQLIITV
LMLIGSLVLITGLDEKKSSGLTVALMLMGLASVSAVILADDLFNIYVFYEIAAIAQTGLVIASGTEKAYRSAFRYLLLGN
FAGSILLLGVAFLLSATGTLNISDMHNFLINNPVTPTIYGGLLMIIIGLCYDSGLPPFHTVKADIYARAKPFIAAMLQTY
SKFVLVALMLVIFKIFGGLSYFTNAHGALIAISILGMVFGVVMALLQTDYRKLLAYHAISQGGYVAAGIALGTPLGIVAG
IFHAINHVIYKSALFIGAHLVERKKVGNLNALGGLLPVIPTTALMVLCAKLAISGVPPFNGFQSKLLLAEAAMNVNMPEL
AIIMIMVSIGTFVSMMKAFYLIYLKPCSEDQLNEYKKVKPSKFGIFSLAVLTVLCILLGVYPNLATDLIYPFANEFGRMW
TL

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788827, Length=400, Percent_Identity=24, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788829, Length=326, Percent_Identity=28.5276073619632, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788831, Length=293, Percent_Identity=25.5972696245734, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI2367154, Length=360, Percent_Identity=27.5, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693160, Length=357, Percent_Identity=24.3697478991597, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788613, Length=330, Percent_Identity=23.9393939393939, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52080; Mature: 52080

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLPLIVVFPMFVAIIFNYLNGKDKLIRPMAFLMALLLLVLPFIGNYGIYNFGAHGLES
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
GLASGISYIYSPMKQLIITVLMLIGSLVLITGLDEKKSSGLTVALMLMGLASVSAVILAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLFNIYVFYEIAAIAQTGLVIASGTEKAYRSAFRYLLLGNFAGSILLLGVAFLLSATGTL
HHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
NISDMHNFLINNPVTPTIYGGLLMIIIGLCYDSGLPPFHTVKADIYARAKPFIAAMLQTY
EHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SKFVLVALMLVIFKIFGGLSYFTNAHGALIAISILGMVFGVVMALLQTDYRKLLAYHAIS
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGGYVAAGIALGTPLGIVAGIFHAINHVIYKSALFIGAHLVERKKVGNLNALGGLLPVIP
CCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TTALMVLCAKLAISGVPPFNGFQSKLLLAEAAMNVNMPELAIIMIMVSIGTFVSMMKAFY
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIYLKPCSEDQLNEYKKVKPSKFGIFSLAVLTVLCILLGVYPNLATDLIYPFANEFGRMW
HHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHC
TL
CC
>Mature Secondary Structure
MNLLPLIVVFPMFVAIIFNYLNGKDKLIRPMAFLMALLLLVLPFIGNYGIYNFGAHGLES
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
GLASGISYIYSPMKQLIITVLMLIGSLVLITGLDEKKSSGLTVALMLMGLASVSAVILAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLFNIYVFYEIAAIAQTGLVIASGTEKAYRSAFRYLLLGNFAGSILLLGVAFLLSATGTL
HHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
NISDMHNFLINNPVTPTIYGGLLMIIIGLCYDSGLPPFHTVKADIYARAKPFIAAMLQTY
EHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SKFVLVALMLVIFKIFGGLSYFTNAHGALIAISILGMVFGVVMALLQTDYRKLLAYHAIS
HHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGGYVAAGIALGTPLGIVAGIFHAINHVIYKSALFIGAHLVERKKVGNLNALGGLLPVIP
CCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TTALMVLCAKLAISGVPPFNGFQSKLLLAEAAMNVNMPELAIIMIMVSIGTFVSMMKAFY
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIYLKPCSEDQLNEYKKVKPSKFGIFSLAVLTVLCILLGVYPNLATDLIYPFANEFGRMW
HHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHC
TL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]