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The map label for this gene is hycG [C]

Identifier: 150399680

GI number: 150399680

Start: 949208

End: 949651

Strand: Reverse

Name: hycG [C]

Synonym: Mevan_0931

Alternate gene names: 150399680

Gene position: 949651-949208 (Counterclockwise)

Preceding gene: 150399681

Following gene: 150399679

Centisome position: 55.21

GC content: 38.29

Gene sequence:

>444_bases
ATGTTAAAAGAGCTTTCAAGAAAAAAAAATATTCATATAATGCTTGTTTATACTGGGGGCTGTAACGGCTGTGATATTGA
AGTGGTAAACTGCGTACTATCTCCATTTTATGATGCGGAACAGTACAACGTATTTTTAACATGGAATCCAAGGGAAGCAG
ACATTTTAGTTGTTACGGGCTGCGTTACAAAATCTGTTACAAAATCTTTAAAAAAGATATACGAAGAAATTCCAAACCCA
AAAGCAGTAGTGTCAGTTGGAGCATGTGCGCTTATGGGTGGAGTTTATAATAATATAGGTGCAGATCTTGGAACATCCAC
TTTTGTTGACGGGCCGGTTGAAAAAATAATTCCAGTAGACGTTAAGGTTCCAGGCTGCCCTCCAAGACCTGAAGACGTGA
TTACTGGGATAGTAAAGGCTCTTCCCAAAATCTTAAATGGATGA

Upstream 100 bases:

>100_bases
TGAACAGCTTAGAAAAATTGCAAAAATTCTTTCAATAAATATGTCAGGAATAGTAAATCGGGAAAAGAAAGCTAGCACAA
AAATTTAAAGGTGTCTTAAG

Downstream 100 bases:

>100_bases
GGAGATAGTCATGTTTGAAAGCATTTTAACATTAGAATCGTTTGAAATTATTCTTTCAATAATGGGGGTTCCATTAATAG
CTTTTGCAATTTCAACTTGG

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLKELSRKKNIHIMLVYTGGCNGCDIEVVNCVLSPFYDAEQYNVFLTWNPREADILVVTGCVTKSVTKSLKKIYEEIPNP
KAVVSVGACALMGGVYNNIGADLGTSTFVDGPVEKIIPVDVKVPGCPPRPEDVITGIVKALPKILNG

Sequences:

>Translated_147_residues
MLKELSRKKNIHIMLVYTGGCNGCDIEVVNCVLSPFYDAEQYNVFLTWNPREADILVVTGCVTKSVTKSLKKIYEEIPNP
KAVVSVGACALMGGVYNNIGADLGTSTFVDGPVEKIIPVDVKVPGCPPRPEDVITGIVKALPKILNG
>Mature_147_residues
MLKELSRKKNIHIMLVYTGGCNGCDIEVVNCVLSPFYDAEQYNVFLTWNPREADILVVTGCVTKSVTKSLKKIYEEIPNP
KAVVSVGACALMGGVYNNIGADLGTSTFVDGPVEKIIPVDVKVPGCPPRPEDVITGIVKALPKILNG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=32.8671328671329, Blast_Score=93, Evalue=9e-20,
Organism=Escherichia coli, GI1789074, Length=141, Percent_Identity=38.2978723404255, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788834, Length=134, Percent_Identity=36.5671641791045, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788624, Length=123, Percent_Identity=34.9593495934959, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17509685, Length=127, Percent_Identity=35.4330708661417, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24651058, Length=127, Percent_Identity=36.2204724409449, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859983, Length=127, Percent_Identity=35.4330708661417, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24642371, Length=127, Percent_Identity=35.4330708661417, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 15836; Mature: 15836

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKELSRKKNIHIMLVYTGGCNGCDIEVVNCVLSPFYDAEQYNVFLTWNPREADILVVTG
CCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEC
CVTKSVTKSLKKIYEEIPNPKAVVSVGACALMGGVYNNIGADLGTSTFVDGPVEKIIPVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEE
VKVPGCPPRPEDVITGIVKALPKILNG
EECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKELSRKKNIHIMLVYTGGCNGCDIEVVNCVLSPFYDAEQYNVFLTWNPREADILVVTG
CCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEC
CVTKSVTKSLKKIYEEIPNPKAVVSVGACALMGGVYNNIGADLGTSTFVDGPVEKIIPVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEE
VKVPGCPPRPEDVITGIVKALPKILNG
EECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]