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The map label for this gene is gatA

Identifier: 150399573

GI number: 150399573

Start: 850343

End: 851638

Strand: Reverse

Name: gatA

Synonym: Mevan_0822

Alternate gene names: 150399573

Gene position: 851638-850343 (Counterclockwise)

Preceding gene: 150399574

Following gene: 150399567

Centisome position: 49.51

GC content: 35.34

Gene sequence:

>1296_bases
ATGATTACGGACAGGGTTTCCGATTACTTGGAAAAAATCGAAAAGTCTGATATTAATGCTTTTATCGATTTAAATGGCGA
GAGAGTACTTAAAGAAGCAAAAGACTTGGAAAAAAATGAAAAACTTAAAAATAAATCTTTGTATGGAAAAATAATTGCTG
TAAAGTCAAATATCAATGTTGAAGGATATAAAATTTCATGTGCATCAAAAACTCTTGATAACTACACGGGAACTTACGAT
GCAACAGTAATTAAAAAATTACGGTCGGAAGGGGCTTTAATTATTGGTATGACAAACATGGATGAATTTGCAAGCGGGAG
TAGTGGCGAAACTTCTTATTATGGCCCTACTAAAAACCCTAAAGCACTGGATAGGATTCCTGGCGGTAGTAGTTCAGGTA
GTGCAGCTGCAGTTTCAGCAGATTTATGTGATATGGCACTTGGAAGCGATACTGGGGGAAGTATTAGAAACCCTGCAAGT
CACTGCGGTGTAGTTGGATTTAAGCCCACGTATGGGGTTGTTTCAAGACAGGGTTTATGCGACCTTGCAATGAGTTTTGA
CCAGATTGGGCCGCTTACAAAAACTGCAGAAGATTCCTTGAATTTAACAAATGTTATAAAAGGACTTGATTTATCAAATA
CTACTTCATTAAAAACTCCCAAATTTGAAAAAAAGGAAATTAAAGACTATAAAATAGGAGTAGTTAGGGAATTTATGGAT
GTCACCGACGAAAAAATTAGAAATGAAATTGAAAAGGGAATTGAAGTGTTTAGGGATTTAGGTTGCGAAATTGTGGACTT
AAGTTATAAATACATTGACCTTGCACTTCCAACATACTACCTAATAAACTATGTTGAATTTTATTCCGCAACTAGAAAAT
ATGATGGAAGAAGGTTTGGGGAGGTTATTGAAGAAGCATGTGGTGAGGAAGTTTTAAGAAGGATTTTAATTGGAAAACAC
ATTAGTGAACAAGAGTTTAGTGGCAAATATTACAAAAAAGCGCTCCAAGCAAGGAAAGAAATGAAAAAAGAAATGATTGG
ATTATTTAATAGTGTAGATTTAATTGTGGGGCCAACAGTCCCTAAATTACCCCATAAAATTGGTGAGGAACTTTCCCCCA
TGGAAATGTATGCATACGACGTTCTAACGGTTCTTACAAATATTTGTGGGATATGTTCAGGAGTTGTTGGCTGCGGAAAT
ATTAATGGAATTCCTGTTGGCCTACAAATTCAAGGGGCGTCCCTTGACGATGAAAAAGTATTAAGTGCAATGATTGAATT
TGAAAATAATTATTAA

Upstream 100 bases:

>100_bases
TCAGTCGGATTTATAATTTTATTTAATTATTGTTATTTTCTCAATACTTTTTTATACGTAAAATGAATAACGTTATAGGA
CTTGTAATTTAGGTGATTGA

Downstream 100 bases:

>100_bases
ATTTTTATTTGAAATTACATAAATTATAAAAATTTATCTTTAATATTTTTTAGGGTTTACAAAAATTACATGCAATTTTT
ATAAATAATTTATATATTGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MITDRVSDYLEKIEKSDINAFIDLNGERVLKEAKDLEKNEKLKNKSLYGKIIAVKSNINVEGYKISCASKTLDNYTGTYD
ATVIKKLRSEGALIIGMTNMDEFASGSSGETSYYGPTKNPKALDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPAS
HCGVVGFKPTYGVVSRQGLCDLAMSFDQIGPLTKTAEDSLNLTNVIKGLDLSNTTSLKTPKFEKKEIKDYKIGVVREFMD
VTDEKIRNEIEKGIEVFRDLGCEIVDLSYKYIDLALPTYYLINYVEFYSATRKYDGRRFGEVIEEACGEEVLRRILIGKH
ISEQEFSGKYYKKALQARKEMKKEMIGLFNSVDLIVGPTVPKLPHKIGEELSPMEMYAYDVLTVLTNICGICSGVVGCGN
INGIPVGLQIQGASLDDEKVLSAMIEFENNY

Sequences:

>Translated_431_residues
MITDRVSDYLEKIEKSDINAFIDLNGERVLKEAKDLEKNEKLKNKSLYGKIIAVKSNINVEGYKISCASKTLDNYTGTYD
ATVIKKLRSEGALIIGMTNMDEFASGSSGETSYYGPTKNPKALDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPAS
HCGVVGFKPTYGVVSRQGLCDLAMSFDQIGPLTKTAEDSLNLTNVIKGLDLSNTTSLKTPKFEKKEIKDYKIGVVREFMD
VTDEKIRNEIEKGIEVFRDLGCEIVDLSYKYIDLALPTYYLINYVEFYSATRKYDGRRFGEVIEEACGEEVLRRILIGKH
ISEQEFSGKYYKKALQARKEMKKEMIGLFNSVDLIVGPTVPKLPHKIGEELSPMEMYAYDVLTVLTNICGICSGVVGCGN
INGIPVGLQIQGASLDDEKVLSAMIEFENNY
>Mature_431_residues
MITDRVSDYLEKIEKSDINAFIDLNGERVLKEAKDLEKNEKLKNKSLYGKIIAVKSNINVEGYKISCASKTLDNYTGTYD
ATVIKKLRSEGALIIGMTNMDEFASGSSGETSYYGPTKNPKALDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPAS
HCGVVGFKPTYGVVSRQGLCDLAMSFDQIGPLTKTAEDSLNLTNVIKGLDLSNTTSLKTPKFEKKEIKDYKIGVVREFMD
VTDEKIRNEIEKGIEVFRDLGCEIVDLSYKYIDLALPTYYLINYVEFYSATRKYDGRRFGEVIEEACGEEVLRRILIGKH
ISEQEFSGKYYKKALQARKEMKKEMIGLFNSVDLIVGPTVPKLPHKIGEELSPMEMYAYDVLTVLTNICGICSGVVGCGN
INGIPVGLQIQGASLDDEKVLSAMIEFENNY

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=464, Percent_Identity=31.0344827586207, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI195972892, Length=470, Percent_Identity=25.7446808510638, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=33.1081081081081, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17537465, Length=454, Percent_Identity=24.4493392070485, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=124, Percent_Identity=37.0967741935484, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=158, Percent_Identity=29.746835443038, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=245, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=245, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=33.411214953271, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=27.5943396226415, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648113, Length=458, Percent_Identity=32.3144104803493, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21356731, Length=218, Percent_Identity=30.7339449541284, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652985, Length=467, Percent_Identity=24.1970021413276, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922090, Length=467, Percent_Identity=24.1970021413276, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652981, Length=467, Percent_Identity=24.1970021413276, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652983, Length=467, Percent_Identity=24.1970021413276, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648435, Length=467, Percent_Identity=25.2676659528908, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648437, Length=467, Percent_Identity=25.2676659528908, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550774, Length=467, Percent_Identity=25.2676659528908, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161078093, Length=476, Percent_Identity=25.4201680672269, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24644968, Length=465, Percent_Identity=24.0860215053763, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648441, Length=413, Percent_Identity=25.4237288135593, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=413, Percent_Identity=25.4237288135593, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_METVS (A6UQF6)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323340.1
- ProteinModelPortal:   A6UQF6
- SMR:   A6UQF6
- STRING:   A6UQF6
- GeneID:   5325110
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0822
- eggNOG:   arNOG04872
- HOGENOM:   HBG481888
- OMA:   ICTVIAN
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 47455; Mature: 47455

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 55-55 ACT_SITE 130-130 ACT_SITE 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDRVSDYLEKIEKSDINAFIDLNGERVLKEAKDLEKNEKLKNKSLYGKIIAVKSNINV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
EGYKISCASKTLDNYTGTYDATVIKKLRSEGALIIGMTNMDEFASGSSGETSYYGPTKNP
CCEEEEEHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCC
KALDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPASHCGVVGFKPTYGVVSRQGLC
HHHHCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHCCCHH
DLAMSFDQIGPLTKTAEDSLNLTNVIKGLDLSNTTSLKTPKFEKKEIKDYKIGVVREFMD
HHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VTDEKIRNEIEKGIEVFRDLGCEIVDLSYKYIDLALPTYYLINYVEFYSATRKYDGRRFG
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EVIEEACGEEVLRRILIGKHISEQEFSGKYYKKALQARKEMKKEMIGLFNSVDLIVGPTV
HHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
PKLPHKIGEELSPMEMYAYDVLTVLTNICGICSGVVGCGNINGIPVGLQIQGASLDDEKV
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHH
LSAMIEFENNY
HHHHHHHCCCC
>Mature Secondary Structure
MITDRVSDYLEKIEKSDINAFIDLNGERVLKEAKDLEKNEKLKNKSLYGKIIAVKSNINV
CCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
EGYKISCASKTLDNYTGTYDATVIKKLRSEGALIIGMTNMDEFASGSSGETSYYGPTKNP
CCEEEEEHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCC
KALDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPASHCGVVGFKPTYGVVSRQGLC
HHHHCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHCCCHH
DLAMSFDQIGPLTKTAEDSLNLTNVIKGLDLSNTTSLKTPKFEKKEIKDYKIGVVREFMD
HHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VTDEKIRNEIEKGIEVFRDLGCEIVDLSYKYIDLALPTYYLINYVEFYSATRKYDGRRFG
HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EVIEEACGEEVLRRILIGKHISEQEFSGKYYKKALQARKEMKKEMIGLFNSVDLIVGPTV
HHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
PKLPHKIGEELSPMEMYAYDVLTVLTNICGICSGVVGCGNINGIPVGLQIQGASLDDEKV
CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHH
LSAMIEFENNY
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA