Click here to switch to the map view.

The map label for this gene is gatD

Identifier: 150399331

GI number: 150399331

Start: 630657

End: 631937

Strand: Reverse

Name: gatD

Synonym: Mevan_0579

Alternate gene names: 150399331

Gene position: 631937-630657 (Counterclockwise)

Preceding gene: 150399332

Following gene: 150399330

Centisome position: 36.74

GC content: 32.01

Gene sequence:

>1281_bases
ATGGATGTCGGCGACTTTGTAAAAATTAAAATGGAGAATACCATGTATTGTGGTACATTAATGCCCTCAATCAATGAAGA
TACAATTGTAGTAAAAATGAAGAGTGGCTATAATGTAGGACTTAAAAAAACCAAAATAAAATCAATTGAAATATTAAAAC
CAGAAAATTCTTCAAATTTTCAACCTTTAACGTCCTCAAATAACGAATTTAAGCCACTAAATTTGGAAAAAAATCCTAAC
TTAAAAAATATTTCAATTTTATCAACTGGGGGGACAGTTGCTTCAAGAGTGGATTATAAAACTGGGGCAGTTCATCCTGC
ATTTACGGCAAACGACTTAATAAGGGCAGTCCCTGAACTTTTAGACATTGCAAATATCAAGGGAAGAGCTATTTTAAACA
TTTTAAGTGAGAACATGCTCCCCAAGTACTGGGTAATGACTGCAGAAGCAATAAAGGAAGAAATCGAAAATGGAGCTGAA
GGGATAGTAATTACCCATGGAACTGACACTATGCACTATACTGCTGCCGCATTATCATTTATGGTAGAATCAGAAGTTCC
AATAATTTTAGTTGGCGCTCAAAGAAGCAGTGATAGGCCGTCATCTGACGCAGCACTAAATATCATTTCAGCAGTAATGG
CTGCAACAGAACCTATAAAAGGGGTTTATGTTGTAATGCACGGGGAAATTGATGATACTATATGTAACTTACACGAAGGG
GTAAAGGTTAGAAAACTTCATTCTTCAAGAAGAGATGCATTTAAATCAGTAAATAACACGCCTGTTGCAAAAATTAATCC
ATTTACTAAAGAAATAACCTATTTAAGAGAGATAAAACCTCAAAACTCGTCAAAAATTAAAAAAGTATCAATAAATAAAA
AATTGGAAGAAAAAATTGCTTTGATAAAAGTTTATCCCGGAATTGATTCAGAAATTTTGAAATTTTACGTCGATAAAGGT
TACAAAGCAATTGTTCTCGAAGGAACTGGCTTAGGGCATACTCCAGAAACTTTTTTTAGTGGTATAGATTATGCAAATAA
AAATAATGTTTTAGTTTTAATGTCGACACAAACTATTAATGGACGGGTAAATATGAATATTTATTCTAATGGGCGGGAAT
TACAGTCTTTAGGGGTACTACCATGTGAAGATATTCTTTCGGAAGTTTTATTTGTTAAAATAATGCACTTACTTGGAAAT
TATGATTTAAAAGATGCTAAAAAATTAATTTGTAAAAATAGGGTAGGAGAAATTAATGAATCGATTAATTTAAAATATTA
A

Upstream 100 bases:

>100_bases
AATAAACTTTAGGGATTAAACATTAAAATCTCTAAGTTTAATTTTTTTATACCATAAATGACAATTATATCCTAGACTAG
TGTTTTTAAAAGGTGGAAAA

Downstream 100 bases:

>100_bases
CATGTAAATTTATAATATTGGTTTAAATTGGATTTATTAATTTAACTTGGATTTTAATAATTTTTATCACTATACTTTAG
ATTTTTGGTGGAAAAATGGA

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MDVGDFVKIKMENTMYCGTLMPSINEDTIVVKMKSGYNVGLKKTKIKSIEILKPENSSNFQPLTSSNNEFKPLNLEKNPN
LKNISILSTGGTVASRVDYKTGAVHPAFTANDLIRAVPELLDIANIKGRAILNILSENMLPKYWVMTAEAIKEEIENGAE
GIVITHGTDTMHYTAAALSFMVESEVPIILVGAQRSSDRPSSDAALNIISAVMAATEPIKGVYVVMHGEIDDTICNLHEG
VKVRKLHSSRRDAFKSVNNTPVAKINPFTKEITYLREIKPQNSSKIKKVSINKKLEEKIALIKVYPGIDSEILKFYVDKG
YKAIVLEGTGLGHTPETFFSGIDYANKNNVLVLMSTQTINGRVNMNIYSNGRELQSLGVLPCEDILSEVLFVKIMHLLGN
YDLKDAKKLICKNRVGEINESINLKY

Sequences:

>Translated_426_residues
MDVGDFVKIKMENTMYCGTLMPSINEDTIVVKMKSGYNVGLKKTKIKSIEILKPENSSNFQPLTSSNNEFKPLNLEKNPN
LKNISILSTGGTVASRVDYKTGAVHPAFTANDLIRAVPELLDIANIKGRAILNILSENMLPKYWVMTAEAIKEEIENGAE
GIVITHGTDTMHYTAAALSFMVESEVPIILVGAQRSSDRPSSDAALNIISAVMAATEPIKGVYVVMHGEIDDTICNLHEG
VKVRKLHSSRRDAFKSVNNTPVAKINPFTKEITYLREIKPQNSSKIKKVSINKKLEEKIALIKVYPGIDSEILKFYVDKG
YKAIVLEGTGLGHTPETFFSGIDYANKNNVLVLMSTQTINGRVNMNIYSNGRELQSLGVLPCEDILSEVLFVKIMHLLGN
YDLKDAKKLICKNRVGEINESINLKY
>Mature_426_residues
MDVGDFVKIKMENTMYCGTLMPSINEDTIVVKMKSGYNVGLKKTKIKSIEILKPENSSNFQPLTSSNNEFKPLNLEKNPN
LKNISILSTGGTVASRVDYKTGAVHPAFTANDLIRAVPELLDIANIKGRAILNILSENMLPKYWVMTAEAIKEEIENGAE
GIVITHGTDTMHYTAAALSFMVESEVPIILVGAQRSSDRPSSDAALNIISAVMAATEPIKGVYVVMHGEIDDTICNLHEG
VKVRKLHSSRRDAFKSVNNTPVAKINPFTKEITYLREIKPQNSSKIKKVSINKKLEEKIALIKVYPGIDSEILKFYVDKG
YKAIVLEGTGLGHTPETFFSGIDYANKNNVLVLMSTQTINGRVNMNIYSNGRELQSLGVLPCEDILSEVLFVKIMHLLGN
YDLKDAKKLICKNRVGEINESINLKY

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=365, Percent_Identity=27.1232876712329, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1788065, Length=289, Percent_Identity=29.757785467128, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789327, Length=274, Percent_Identity=33.5766423357664, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI193207372, Length=369, Percent_Identity=25.2032520325203, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193207374, Length=367, Percent_Identity=25.3405994550409, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558278, Length=367, Percent_Identity=25.3405994550409, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558280, Length=367, Percent_Identity=25.3405994550409, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323189, Length=269, Percent_Identity=31.2267657992565, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323187, Length=269, Percent_Identity=31.2267657992565, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323186, Length=269, Percent_Identity=31.2267657992565, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323184, Length=269, Percent_Identity=31.2267657992565, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320527, Length=352, Percent_Identity=28.125, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24645530, Length=266, Percent_Identity=30.8270676691729, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24639609, Length=289, Percent_Identity=28.3737024221453, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_METVS (A6UPR4)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323098.1
- ProteinModelPortal:   A6UPR4
- SMR:   A6UPR4
- STRING:   A6UPR4
- GeneID:   5326062
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0579
- eggNOG:   arNOG04527
- HOGENOM:   HBG497495
- OMA:   KMKSGYN
- ProtClustDB:   PRK04183
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 47169; Mature: 47169

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 92-92 ACT_SITE 168-168 ACT_SITE 169-169 ACT_SITE 245-245

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVGDFVKIKMENTMYCGTLMPSINEDTIVVKMKSGYNVGLKKTKIKSIEILKPENSSNF
CCCCCEEEEEECCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEECEEEEECCCCCCCC
QPLTSSNNEFKPLNLEKNPNLKNISILSTGGTVASRVDYKTGAVHPAFTANDLIRAVPEL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
LDIANIKGRAILNILSENMLPKYWVMTAEAIKEEIENGAEGIVITHGTDTMHYTAAALSF
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
MVESEVPIILVGAQRSSDRPSSDAALNIISAVMAATEPIKGVYVVMHGEIDDTICNLHEG
HCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCC
VKVRKLHSSRRDAFKSVNNTPVAKINPFTKEITYLREIKPQNSSKIKKVSINKKLEEKIA
CCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCEE
LIKVYPGIDSEILKFYVDKGYKAIVLEGTGLGHTPETFFSGIDYANKNNVLVLMSTQTIN
EEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEEEEEEEEEC
GRVNMNIYSNGRELQSLGVLPCEDILSEVLFVKIMHLLGNYDLKDAKKLICKNRVGEINE
CEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
SINLKY
CCCCCC
>Mature Secondary Structure
MDVGDFVKIKMENTMYCGTLMPSINEDTIVVKMKSGYNVGLKKTKIKSIEILKPENSSNF
CCCCCEEEEEECCEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEECEEEEECCCCCCCC
QPLTSSNNEFKPLNLEKNPNLKNISILSTGGTVASRVDYKTGAVHPAFTANDLIRAVPEL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
LDIANIKGRAILNILSENMLPKYWVMTAEAIKEEIENGAEGIVITHGTDTMHYTAAALSF
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
MVESEVPIILVGAQRSSDRPSSDAALNIISAVMAATEPIKGVYVVMHGEIDDTICNLHEG
HCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCC
VKVRKLHSSRRDAFKSVNNTPVAKINPFTKEITYLREIKPQNSSKIKKVSINKKLEEKIA
CCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCEE
LIKVYPGIDSEILKFYVDKGYKAIVLEGTGLGHTPETFFSGIDYANKNNVLVLMSTQTIN
EEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEEEEEEEEEC
GRVNMNIYSNGRELQSLGVLPCEDILSEVLFVKIMHLLGNYDLKDAKKLICKNRVGEINE
CEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
SINLKY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA