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The map label for this gene is purF [H]

Identifier: 150399220

GI number: 150399220

Start: 520870

End: 522240

Strand: Reverse

Name: purF [H]

Synonym: Mevan_0468

Alternate gene names: 150399220

Gene position: 522240-520870 (Counterclockwise)

Preceding gene: 150399221

Following gene: 150399218

Centisome position: 30.36

GC content: 34.06

Gene sequence:

>1371_bases
ATGTGTGGAATCTTTGGTATTTATTCTCATGAGAAAAGTAATATCGTCAAAAAAGTTTATTACGGGCTTTATGCACTCCA
ACACCGAGGTCAAGAAGGTGCAGGTATTGCAGTAGGTAATGGTAAAGAAATAGGGTATTATAAAGGACTAGGGCTTGTTC
CAGAAGTTTTTTCAAATAAAAATCTTCAAAATTTGTATGGACATATTGGAATTGGCCACGTTAGATACTCTACAACTGGC
GGAAACATAATTGAAAATTGTCAGCCTTTTGTTGTAAATAGCTCTTTTGGAAAAATTGCAATTGCCCATAATGGCGATAT
TGTAAATTCCTCAGAATTAAAGCGAGAACTCGAAAAAAATGGGCATATATTTGTTTCATCAACCGATTCGGAAGTAATTG
CGCAACTTTTAGTAAGGGAACTTTTAAAAGATGACGATATAATATCCGCAATTATGAAAGTTACAGAAAAACTAAATGGT
GCATATTCTCTTTTAATAATTTACGATGATACCCTAATTGCACTAAGGGATCCGAAAGGTTTTAAACCACTTTGTATTGG
AAAAGATGAGGGTGCATACTATTTTAGCTCAGAAAGTTGCGCGCTTGATATAGTAAACGTTGATTTTGAAAAAGATGTTT
CCCCCGGGGAAATGGTTGTTATTAAAAATAATGAAATGAAAACTTACAAACTTCCAAATTCAAATGGCAATGCATCAACA
TGCATGTTTGAATATGTTTATTTTGCAAGACCTGATTCAGTAATTGATGGAATAAGTGTTTATGCCGTTAGGAGAAATAT
TGGGCGGATTCTTGCAAGGGAAAATCCAAATAATTCTGACGTGGTTTCCCCAGTACCTGACTCGGGAATTATATTTTCAC
AAGGATATACTGAAGAAGCAAGTGTTCCATACTACGAAGCTTTAATTAAAAATAGATATATTGGAAGAACATTTATTCTT
CCAACCCAAGAAGAACGAGATTTAGCGGTAAGACTTAAATTAAATCCTGTGAAACACCTTTTAAGGGATAAAAAAGTAAC
ATTAATTGATGACAGTATAGTACGAGGTACTACCTCTGGAAAAATTATAAAAATGGCAAGAAAAGCAGGTGCAAAAGAAG
TACATTTAAGAATAGGCTCTCCAAGAATAGTTTCACCATGTTTTTACGGAATAGACATGGCAACGACAAAGGAATTAATT
GCAAGTTCAAAAACAGATGAAGAAATTTCAGAATGTATTGGTGCAGATTCTGTTGCGTATTTAAGTATTAATGGATTAGT
AGAAGCGATTGGACGAAATGACCTTTGTTTAGCTTGTTTAACTGGGAAATACCCGACAGACGTTTCATGTAAGTTTAACT
GCGAAAAATAA

Upstream 100 bases:

>100_bases
ACCATTTCTTTTTTACTTAAAATTAAATCCTGACTACCTTTTTTGAAACGCTTAAATATTAGTTTAAGTTAGTTATTCTA
TCAATTTTATTCGTGATATC

Downstream 100 bases:

>100_bases
TATATATTTTAAAATTTAAAAAATAATTATTTTTTAAATTTTGAAAAAATGCTTCTTTTTAATTTTTCAAAAAAACTTTC
ATTATATATCTCTTCAATTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MCGIFGIYSHEKSNIVKKVYYGLYALQHRGQEGAGIAVGNGKEIGYYKGLGLVPEVFSNKNLQNLYGHIGIGHVRYSTTG
GNIIENCQPFVVNSSFGKIAIAHNGDIVNSSELKRELEKNGHIFVSSTDSEVIAQLLVRELLKDDDIISAIMKVTEKLNG
AYSLLIIYDDTLIALRDPKGFKPLCIGKDEGAYYFSSESCALDIVNVDFEKDVSPGEMVVIKNNEMKTYKLPNSNGNAST
CMFEYVYFARPDSVIDGISVYAVRRNIGRILARENPNNSDVVSPVPDSGIIFSQGYTEEASVPYYEALIKNRYIGRTFIL
PTQEERDLAVRLKLNPVKHLLRDKKVTLIDDSIVRGTTSGKIIKMARKAGAKEVHLRIGSPRIVSPCFYGIDMATTKELI
ASSKTDEEISECIGADSVAYLSINGLVEAIGRNDLCLACLTGKYPTDVSCKFNCEK

Sequences:

>Translated_456_residues
MCGIFGIYSHEKSNIVKKVYYGLYALQHRGQEGAGIAVGNGKEIGYYKGLGLVPEVFSNKNLQNLYGHIGIGHVRYSTTG
GNIIENCQPFVVNSSFGKIAIAHNGDIVNSSELKRELEKNGHIFVSSTDSEVIAQLLVRELLKDDDIISAIMKVTEKLNG
AYSLLIIYDDTLIALRDPKGFKPLCIGKDEGAYYFSSESCALDIVNVDFEKDVSPGEMVVIKNNEMKTYKLPNSNGNAST
CMFEYVYFARPDSVIDGISVYAVRRNIGRILARENPNNSDVVSPVPDSGIIFSQGYTEEASVPYYEALIKNRYIGRTFIL
PTQEERDLAVRLKLNPVKHLLRDKKVTLIDDSIVRGTTSGKIIKMARKAGAKEVHLRIGSPRIVSPCFYGIDMATTKELI
ASSKTDEEISECIGADSVAYLSINGLVEAIGRNDLCLACLTGKYPTDVSCKFNCEK
>Mature_456_residues
MCGIFGIYSHEKSNIVKKVYYGLYALQHRGQEGAGIAVGNGKEIGYYKGLGLVPEVFSNKNLQNLYGHIGIGHVRYSTTG
GNIIENCQPFVVNSSFGKIAIAHNGDIVNSSELKRELEKNGHIFVSSTDSEVIAQLLVRELLKDDDIISAIMKVTEKLNG
AYSLLIIYDDTLIALRDPKGFKPLCIGKDEGAYYFSSESCALDIVNVDFEKDVSPGEMVVIKNNEMKTYKLPNSNGNAST
CMFEYVYFARPDSVIDGISVYAVRRNIGRILARENPNNSDVVSPVPDSGIIFSQGYTEEASVPYYEALIKNRYIGRTFIL
PTQEERDLAVRLKLNPVKHLLRDKKVTLIDDSIVRGTTSGKIIKMARKAGAKEVHLRIGSPRIVSPCFYGIDMATTKELI
ASSKTDEEISECIGADSVAYLSINGLVEAIGRNDLCLACLTGKYPTDVSCKFNCEK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=504, Percent_Identity=40.4761904761905, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI4826742, Length=225, Percent_Identity=29.3333333333333, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI205277386, Length=191, Percent_Identity=27.7486910994764, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788651, Length=451, Percent_Identity=43.0155210643016, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1790167, Length=213, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=40.5405405405405, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17532899, Length=185, Percent_Identity=31.8918918918919, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532897, Length=185, Percent_Identity=31.8918918918919, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539970, Length=185, Percent_Identity=31.8918918918919, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323958, Length=475, Percent_Identity=38.5263157894737, Blast_Score=301, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322745, Length=246, Percent_Identity=30.8943089430894, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323730, Length=209, Percent_Identity=32.0574162679426, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=40.2489626556017, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24659604, Length=458, Percent_Identity=41.4847161572052, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI28573187, Length=486, Percent_Identity=40.3292181069959, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI21357745, Length=184, Percent_Identity=29.3478260869565, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50239; Mature: 50239

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGIYSHEKSNIVKKVYYGLYALQHRGQEGAGIAVGNGKEIGYYKGLGLVPEVFSNK
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEECEECCCCCCHHHHCCC
NLQNLYGHIGIGHVRYSTTGGNIIENCQPFVVNSSFGKIAIAHNGDIVNSSELKRELEKN
CHHHHHHCCEEEEEEEECCCCCHHHCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHC
GHIFVSSTDSEVIAQLLVRELLKDDDIISAIMKVTEKLNGAYSLLIIYDDTLIALRDPKG
CCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECCCC
FKPLCIGKDEGAYYFSSESCALDIVNVDFEKDVSPGEMVVIKNNEMKTYKLPNSNGNAST
CCCEEEECCCCCEEECCCCEEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCE
CMFEYVYFARPDSVIDGISVYAVRRNIGRILARENPNNSDVVSPVPDSGIIFSQGYTEEA
EEEEEEEEECCCHHHCCHHHEEHHHHHHHHEECCCCCCCCCCCCCCCCCEEEECCCCCCC
SVPYYEALIKNRYIGRTFILPTQEERDLAVRLKLNPVKHLLRDKKVTLIDDSIVRGTTSG
CCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECHHHHHHCCCCEEEEECCCCCCCCCC
KIIKMARKAGAKEVHLRIGSPRIVSPCFYGIDMATTKELIASSKTDEEISECIGADSVAY
HHHHHHHHCCCEEEEEEECCCCEECHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEE
LSINGLVEAIGRNDLCLACLTGKYPTDVSCKFNCEK
EEEHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCC
>Mature Secondary Structure
MCGIFGIYSHEKSNIVKKVYYGLYALQHRGQEGAGIAVGNGKEIGYYKGLGLVPEVFSNK
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEECEECCCCCCHHHHCCC
NLQNLYGHIGIGHVRYSTTGGNIIENCQPFVVNSSFGKIAIAHNGDIVNSSELKRELEKN
CHHHHHHCCEEEEEEEECCCCCHHHCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHC
GHIFVSSTDSEVIAQLLVRELLKDDDIISAIMKVTEKLNGAYSLLIIYDDTLIALRDPKG
CCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECCCC
FKPLCIGKDEGAYYFSSESCALDIVNVDFEKDVSPGEMVVIKNNEMKTYKLPNSNGNAST
CCCEEEECCCCCEEECCCCEEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCE
CMFEYVYFARPDSVIDGISVYAVRRNIGRILARENPNNSDVVSPVPDSGIIFSQGYTEEA
EEEEEEEEECCCHHHCCHHHEEHHHHHHHHEECCCCCCCCCCCCCCCCCEEEECCCCCCC
SVPYYEALIKNRYIGRTFILPTQEERDLAVRLKLNPVKHLLRDKKVTLIDDSIVRGTTSG
CCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECHHHHHHCCCCEEEEECCCCCCCCCC
KIIKMARKAGAKEVHLRIGSPRIVSPCFYGIDMATTKELIASSKTDEEISECIGADSVAY
HHHHHHHHCCCEEEEEEECCCCEECHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEE
LSINGLVEAIGRNDLCLACLTGKYPTDVSCKFNCEK
EEEHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]