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The map label for this gene is prf1

Identifier: 150399205

GI number: 150399205

Start: 506124

End: 507383

Strand: Reverse

Name: prf1

Synonym: Mevan_0453

Alternate gene names: 150399205

Gene position: 507383-506124 (Counterclockwise)

Preceding gene: 150399211

Following gene: 150399204

Centisome position: 29.5

GC content: 33.65

Gene sequence:

>1260_bases
ATGAGCGAAAATTCATCAACGGACATGTATTTATTTAAAAAATCATTAAAAGAACTAAAAGGAAAGCGTGGAAAGGGGAC
AGAGCTTATAAGTGTATACGTTCCAGCAGGAAGACGTCTTTCGGACATTTCTCAGCATTTAAGGCAAGAACTTTCACAGT
CTTCAAATATAAAAAGTAAAACAACAATGAAAAACGTACAGTCAGCAATAGAGGTAATTTTACAGAGATTAAAACTGTTA
AAAGAACCTTTAGAAAAAGGAGTTATTATATTTGCAGGAATGATTCCAAGAGGAGGCCCTGGAACAGAAAAAATGGAAGT
ATATGTTCTTGAACCGCCAGAACCTGTAAAAACGTTTATTTATCGATGTGACTCTGAATTTTATACTGAATCTTTAGAAG
ACTTTATTCAGGATACTGAAGTTTATGGGGTAATTTTGGTAGATAGAAATGAGGCTACAATTGGAACTGTTAAGGGAAAA
ACGATAACCGTTCTAAAGAAATTAACCAGCGGTGTTCCAGGAAAATTCAAGGCGGGAGGGCAATCTGCAAGGAGACTTGA
GCGACTTATCGATGATGCGGCCCACCAATTCATGGTAAGAATTGGGGAATATTCAACAGAATCGTTCATGCCAATTTTAG
AAGAGAAAAAACTAAGGGGTCTGTTGATAGGTGGCCCAGGAAATACTAAAAATGAATTTGCAGAAAAAGACTTTTTGCAC
CATGAATTAAAGAAAAAAATTATCGATACATTTGACCTCTGTTATACTGAGGAATTTGGTATAAGGGAGCTTTTAGAAAA
AGCTTCAGATCTTTTAAGGGATTTAGATTTGATGAAGGAGAAAAATTTAATTCAAAAATTCTTTAAAGAGTTAATAAAAG
ATGACGGTGGCCTTTCAGCATACGGTGAATCGCAGGTAATGAGATATCTTGAAATGGGCGCAATAGATACCTTGATAGTT
ACAGAAGATATTGAATTAACAAGGGTTACAATAAAATGTAATAACTGTAACTTTAAACAGGAAATAAATGTAAAAACAAG
GGACCTTTATAAATTTGAAGATGAGGTAAAGACAAAAATCTGTAAACAATGTTCCGGTTCAATGTATATTGAAGAAGAAA
AAGACATTATTGAGTATCTATCGGAACTTTGTAACATTCATAATTCTGATATAGTCGTAGTTTCAACAGATACTGAAGAA
GGAAGCCAAATTTCAAAGGCATTTAAAGGCATGGCTGCAATTTTAAGATATAAAATATAA

Upstream 100 bases:

>100_bases
TTCAGTAGCAATAGCTTTTTTACACTAATTATTGTAATAATTATAAAAATAATTGTAAATTAATATATTTAAAGCGATGA
CTATTTTTTAAGGTGTGTAT

Downstream 100 bases:

>100_bases
ATTACCAATCTAACACTTTTTAACTATTTTGGATAAATTGCATATATTTTGGATTTTATTGGTGGTTTTCATGTTTTTTG
AGTATTCGCTAAAAACTAAG

Product: peptide chain release factor 1

Products: GDP; phosphate

Alternate protein names: Translation termination factor aRF1

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSENSSTDMYLFKKSLKELKGKRGKGTELISVYVPAGRRLSDISQHLRQELSQSSNIKSKTTMKNVQSAIEVILQRLKLL
KEPLEKGVIIFAGMIPRGGPGTEKMEVYVLEPPEPVKTFIYRCDSEFYTESLEDFIQDTEVYGVILVDRNEATIGTVKGK
TITVLKKLTSGVPGKFKAGGQSARRLERLIDDAAHQFMVRIGEYSTESFMPILEEKKLRGLLIGGPGNTKNEFAEKDFLH
HELKKKIIDTFDLCYTEEFGIRELLEKASDLLRDLDLMKEKNLIQKFFKELIKDDGGLSAYGESQVMRYLEMGAIDTLIV
TEDIELTRVTIKCNNCNFKQEINVKTRDLYKFEDEVKTKICKQCSGSMYIEEEKDIIEYLSELCNIHNSDIVVVSTDTEE
GSQISKAFKGMAAILRYKI

Sequences:

>Translated_419_residues
MSENSSTDMYLFKKSLKELKGKRGKGTELISVYVPAGRRLSDISQHLRQELSQSSNIKSKTTMKNVQSAIEVILQRLKLL
KEPLEKGVIIFAGMIPRGGPGTEKMEVYVLEPPEPVKTFIYRCDSEFYTESLEDFIQDTEVYGVILVDRNEATIGTVKGK
TITVLKKLTSGVPGKFKAGGQSARRLERLIDDAAHQFMVRIGEYSTESFMPILEEKKLRGLLIGGPGNTKNEFAEKDFLH
HELKKKIIDTFDLCYTEEFGIRELLEKASDLLRDLDLMKEKNLIQKFFKELIKDDGGLSAYGESQVMRYLEMGAIDTLIV
TEDIELTRVTIKCNNCNFKQEINVKTRDLYKFEDEVKTKICKQCSGSMYIEEEKDIIEYLSELCNIHNSDIVVVSTDTEE
GSQISKAFKGMAAILRYKI
>Mature_418_residues
SENSSTDMYLFKKSLKELKGKRGKGTELISVYVPAGRRLSDISQHLRQELSQSSNIKSKTTMKNVQSAIEVILQRLKLLK
EPLEKGVIIFAGMIPRGGPGTEKMEVYVLEPPEPVKTFIYRCDSEFYTESLEDFIQDTEVYGVILVDRNEATIGTVKGKT
ITVLKKLTSGVPGKFKAGGQSARRLERLIDDAAHQFMVRIGEYSTESFMPILEEKKLRGLLIGGPGNTKNEFAEKDFLHH
ELKKKIIDTFDLCYTEEFGIRELLEKASDLLRDLDLMKEKNLIQKFFKELIKDDGGLSAYGESQVMRYLEMGAIDTLIVT
EDIELTRVTIKCNNCNFKQEINVKTRDLYKFEDEVKTKICKQCSGSMYIEEEKDIIEYLSELCNIHNSDIVVVSTDTEEG
SQISKAFKGMAAILRYKI

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family

Homologues:

Organism=Homo sapiens, GI4759034, Length=421, Percent_Identity=29.2161520190024, Blast_Score=209, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI72000368, Length=415, Percent_Identity=30.8433734939759, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319619, Length=416, Percent_Identity=30.0480769230769, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI28574632, Length=419, Percent_Identity=31.0262529832936, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI21356433, Length=419, Percent_Identity=31.0262529832936, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24667392, Length=419, Percent_Identity=31.0262529832936, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24667388, Length=419, Percent_Identity=31.0262529832936, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24667380, Length=419, Percent_Identity=31.0262529832936, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24667384, Length=419, Percent_Identity=31.0262529832936, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF1_METVS (A6UPD8)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322972.1
- ProteinModelPortal:   A6UPD8
- SMR:   A6UPD8
- STRING:   A6UPD8
- GeneID:   5325327
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0453
- eggNOG:   arNOG04578
- HOGENOM:   HBG497188
- OMA:   GDYLHHE
- ProtClustDB:   PRK04011
- GO:   GO:0005737
- HAMAP:   MF_00424
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403
- PANTHER:   PTHR10113
- PANTHER:   PTHR10113:SF2
- TIGRFAMs:   TIGR03676

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: 3.6.5.3

Molecular weight: Translated: 47636; Mature: 47504

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSSTDMYLFKKSLKELKGKRGKGTELISVYVPAGRRLSDISQHLRQELSQSSNIKSK
CCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHH
TTMKNVQSAIEVILQRLKLLKEPLEKGVIIFAGMIPRGGPGTEKMEVYVLEPPEPVKTFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHH
YRCDSEFYTESLEDFIQDTEVYGVILVDRNEATIGTVKGKTITVLKKLTSGVPGKFKAGG
HHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCCC
QSARRLERLIDDAAHQFMVRIGEYSTESFMPILEEKKLRGLLIGGPGNTKNEFAEKDFLH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
HELKKKIIDTFDLCYTEEFGIRELLEKASDLLRDLDLMKEKNLIQKFFKELIKDDGGLSA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YGESQVMRYLEMGAIDTLIVTEDIELTRVTIKCNNCNFKQEINVKTRDLYKFEDEVKTKI
CCHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
CKQCSGSMYIEEEKDIIEYLSELCNIHNSDIVVVSTDTEEGSQISKAFKGMAAILRYKI
HHHCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SENSSTDMYLFKKSLKELKGKRGKGTELISVYVPAGRRLSDISQHLRQELSQSSNIKSK
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHH
TTMKNVQSAIEVILQRLKLLKEPLEKGVIIFAGMIPRGGPGTEKMEVYVLEPPEPVKTFI
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHH
YRCDSEFYTESLEDFIQDTEVYGVILVDRNEATIGTVKGKTITVLKKLTSGVPGKFKAGG
HHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCCC
QSARRLERLIDDAAHQFMVRIGEYSTESFMPILEEKKLRGLLIGGPGNTKNEFAEKDFLH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
HELKKKIIDTFDLCYTEEFGIRELLEKASDLLRDLDLMKEKNLIQKFFKELIKDDGGLSA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YGESQVMRYLEMGAIDTLIVTEDIELTRVTIKCNNCNFKQEINVKTRDLYKFEDEVKTKI
CCHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
CKQCSGSMYIEEEKDIIEYLSELCNIHNSDIVVVSTDTEEGSQISKAFKGMAAILRYKI
HHHCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA