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The map label for this gene is ppsA [H]

Identifier: 150399172

GI number: 150399172

Start: 472742

End: 475015

Strand: Reverse

Name: ppsA [H]

Synonym: Mevan_0419

Alternate gene names: 150399172

Gene position: 475015-472742 (Counterclockwise)

Preceding gene: 150399178

Following gene: 150399171

Centisome position: 27.62

GC content: 35.58

Gene sequence:

>2274_bases
ATGAAACTGATAACATGGCTTAAAGACCTTAGAAATGAAGATGTAGATATTGCTGGTGGAAAAGGAGCATCGCTTGGTGA
AATGTGGAATGCAAAATTACCGGTTCCCCCCGCATTTGTTGTTACTTCTGACGCTTACAAATACTTTATTGAAGAAACGA
AGTTAAAAGATGAAATTAAACGAGTTTTATCAAATATTGACGTTAATAATACTGAAGAATTGATTTCTGCTTCAAAAGAA
GTTAGGGGCGCAATTGAAAAGGTAAAATTGCCAGATGATTTAAAAATCAGTGTTATCGAGGCATACAATACACTTTGTGA
AATGGGTGGTGAGGAGGAATCTTTTGTTGCAGTTAGAAGTTCAGCAACTGCGGAAGATTTACCTGATGCAAGTTTTGCAG
GACAACAGGAAACTTTTTTAAACATGAAAGGAAATGAACAGGTTTTAAAAGCAGTTCAGGAATGTTTTTCATCATTGTTT
ACTCCAAGAGCTATTTTTTACAGGGAAGAGAAAGGTTTTGACCATTTTCAAGTTGCACTTGCTGCAGTAGTTCAAAAAAT
GATTAATGCAGATCAAGCAGGCGTAATGTTTACGGTAAACCCAATAAACCACGATTACAATCAAATGGTAATTGAAGGTG
CATGGGGACTTGGTGAAGGCGTAGTTAGTGGAACGGTAAGCCCTGACACGTATATTATTGAAAAAAATACTGAAAAAATT
GTGGATATAAACGTTGCAAGAAAAAATACGATGTTTATAAAAGAAGATGGCGTTACAAAAGAAGTTGAAACTCCAGAGGA
TTTAAAAGAAAAACAGGTTTTAACAGAAGAATTAATTTTAAATCTTCAAAAAGTAGGTAAAACAATTGAAAACCATTATA
AAATGCCAATGGATATCGAGTGGGCAATTAAAAATGGGGAAATTTACATGCTTCAGGCAAGGCCAATTACAACACTTGGT
GGGAAAAAAGAGGTAAAACAATCCCTAGATCAAGATGGAAAAATATTATTAAAAGGAATTGGTGCATCTCCTGGAATTGA
CTTTGGAAAAGTAAAAATAATTTCAGAAATTGAACAGATTGGAAAAATTTTGGAAGGAGATGTTTTAGTTACAAAAATGA
CGACCCCTGACATGGTTCCTGCAATGAAAAGGGCAAGTGCAATTGTAACTGATGATGGAGGATTAACTTGCCATGCTGCA
ATTATTTCAAGAGAGCTTGGAACTCCTTGTGTAGTTGGAACAAAAGAAGCTACAAAAGTTTTAAAAGATAATTCTTGTGT
AACTGTTGATGGCGAAAAAGGAATGGTATACGACGGTAAAATCGGTAAAAAGGAAGAGAATATTCAAACTCAGGTAATTT
ACGCCGGTTCACAAGTAATTACTGCAACAGAAATAAAAGCAAATGTAAGTATGCCTGAAGTTGCAGAACGGGCTGCCGCA
ACAGGAGCTGACGGTGTAGGCCTTTTAAGGGCAGAACACATGATTTTAGGAACTGGAGTACACCCTGGAAAAATCTTGAA
AGATAAGGGGGAAGATGCATTAATTGAAGTATTTTCTGAAGGAATTAGAAAAGTTGCAGATGCGTTTTACCCAAGGCCTG
TTACATATAGAACACTTGACGCTCCAACTGATGAATTTGTGGGTTTAAAAGGGGGAGAAGATGAACCTCACGAACACAAC
CCGATGATGGGGTGGAGAGGAATTAGAAGGGGACTTGATCAACCTGAAATTTTAAAATGTGAATTAAAAGCAATCAAAAA
ACTAAGGGAAGAAGGTTACCGAAATATCAATGTAATGATTCCATTAGTTACAAATGTTGATGAAATTAGAAAGGTAAAGG
AAATTTCAAATGAAGTGGGTTTAAAATTAATGCAAGACGTGGAATTTGGCGTAATGATTGAAACCCCTGCTGCAGCTTTA
ATAATTGATGAGATTATAAAAGAAGGAATTGACTTTGCAAGCCTTGGAACAAACGATTTAACTCAGTACACAATTGCAAT
TGATAGAAACAATGAAAACGTTTCAAAATATTATCTTGAAAACCACCCTGCAGTTATGAAACTAATTGAACTTGTAATAA
CCTCATGCAAAAGTGCAGGTGTTAAAACTTCAATCTGTGGGCAAGCTGGAAGCCGACCACAAATCGTTGAAAAATTAGTT
GAATGGGGAATTACAAGTATTTCTGCAAATATTGACGCAGTAGAAACAATTAGAAACGTTGTTGCAAGAACTGAACAAAA
AATTATTCTTGAAGCAATAAGGAAGCAGAAATAG

Upstream 100 bases:

>100_bases
GACTCTACAAAATATTATATATCTGAGTAGTGTAAATAGAAAATGTGATTTTAGTTATTTTTATTAAAGAAACGTGAAAA
GCGCCCATGGAGGATTTAAA

Downstream 100 bases:

>100_bases
TTTTTAAATAATTTAGTTATTTTTTTAAAATTATTTTTAAAATTATTTTTAACATCATAAAATTATTTTTAAAATATATT
TAATTTACAGTTATTAATTA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MKLITWLKDLRNEDVDIAGGKGASLGEMWNAKLPVPPAFVVTSDAYKYFIEETKLKDEIKRVLSNIDVNNTEELISASKE
VRGAIEKVKLPDDLKISVIEAYNTLCEMGGEEESFVAVRSSATAEDLPDASFAGQQETFLNMKGNEQVLKAVQECFSSLF
TPRAIFYREEKGFDHFQVALAAVVQKMINADQAGVMFTVNPINHDYNQMVIEGAWGLGEGVVSGTVSPDTYIIEKNTEKI
VDINVARKNTMFIKEDGVTKEVETPEDLKEKQVLTEELILNLQKVGKTIENHYKMPMDIEWAIKNGEIYMLQARPITTLG
GKKEVKQSLDQDGKILLKGIGASPGIDFGKVKIISEIEQIGKILEGDVLVTKMTTPDMVPAMKRASAIVTDDGGLTCHAA
IISRELGTPCVVGTKEATKVLKDNSCVTVDGEKGMVYDGKIGKKEENIQTQVIYAGSQVITATEIKANVSMPEVAERAAA
TGADGVGLLRAEHMILGTGVHPGKILKDKGEDALIEVFSEGIRKVADAFYPRPVTYRTLDAPTDEFVGLKGGEDEPHEHN
PMMGWRGIRRGLDQPEILKCELKAIKKLREEGYRNINVMIPLVTNVDEIRKVKEISNEVGLKLMQDVEFGVMIETPAAAL
IIDEIIKEGIDFASLGTNDLTQYTIAIDRNNENVSKYYLENHPAVMKLIELVITSCKSAGVKTSICGQAGSRPQIVEKLV
EWGITSISANIDAVETIRNVVARTEQKIILEAIRKQK

Sequences:

>Translated_757_residues
MKLITWLKDLRNEDVDIAGGKGASLGEMWNAKLPVPPAFVVTSDAYKYFIEETKLKDEIKRVLSNIDVNNTEELISASKE
VRGAIEKVKLPDDLKISVIEAYNTLCEMGGEEESFVAVRSSATAEDLPDASFAGQQETFLNMKGNEQVLKAVQECFSSLF
TPRAIFYREEKGFDHFQVALAAVVQKMINADQAGVMFTVNPINHDYNQMVIEGAWGLGEGVVSGTVSPDTYIIEKNTEKI
VDINVARKNTMFIKEDGVTKEVETPEDLKEKQVLTEELILNLQKVGKTIENHYKMPMDIEWAIKNGEIYMLQARPITTLG
GKKEVKQSLDQDGKILLKGIGASPGIDFGKVKIISEIEQIGKILEGDVLVTKMTTPDMVPAMKRASAIVTDDGGLTCHAA
IISRELGTPCVVGTKEATKVLKDNSCVTVDGEKGMVYDGKIGKKEENIQTQVIYAGSQVITATEIKANVSMPEVAERAAA
TGADGVGLLRAEHMILGTGVHPGKILKDKGEDALIEVFSEGIRKVADAFYPRPVTYRTLDAPTDEFVGLKGGEDEPHEHN
PMMGWRGIRRGLDQPEILKCELKAIKKLREEGYRNINVMIPLVTNVDEIRKVKEISNEVGLKLMQDVEFGVMIETPAAAL
IIDEIIKEGIDFASLGTNDLTQYTIAIDRNNENVSKYYLENHPAVMKLIELVITSCKSAGVKTSICGQAGSRPQIVEKLV
EWGITSISANIDAVETIRNVVARTEQKIILEAIRKQK
>Mature_757_residues
MKLITWLKDLRNEDVDIAGGKGASLGEMWNAKLPVPPAFVVTSDAYKYFIEETKLKDEIKRVLSNIDVNNTEELISASKE
VRGAIEKVKLPDDLKISVIEAYNTLCEMGGEEESFVAVRSSATAEDLPDASFAGQQETFLNMKGNEQVLKAVQECFSSLF
TPRAIFYREEKGFDHFQVALAAVVQKMINADQAGVMFTVNPINHDYNQMVIEGAWGLGEGVVSGTVSPDTYIIEKNTEKI
VDINVARKNTMFIKEDGVTKEVETPEDLKEKQVLTEELILNLQKVGKTIENHYKMPMDIEWAIKNGEIYMLQARPITTLG
GKKEVKQSLDQDGKILLKGIGASPGIDFGKVKIISEIEQIGKILEGDVLVTKMTTPDMVPAMKRASAIVTDDGGLTCHAA
IISRELGTPCVVGTKEATKVLKDNSCVTVDGEKGMVYDGKIGKKEENIQTQVIYAGSQVITATEIKANVSMPEVAERAAA
TGADGVGLLRAEHMILGTGVHPGKILKDKGEDALIEVFSEGIRKVADAFYPRPVTYRTLDAPTDEFVGLKGGEDEPHEHN
PMMGWRGIRRGLDQPEILKCELKAIKKLREEGYRNINVMIPLVTNVDEIRKVKEISNEVGLKLMQDVEFGVMIETPAAAL
IIDEIIKEGIDFASLGTNDLTQYTIAIDRNNENVSKYYLENHPAVMKLIELVITSCKSAGVKTSICGQAGSRPQIVEKLV
EWGITSISANIDAVETIRNVVARTEQKIILEAIRKQK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=783, Percent_Identity=38.1864623243934, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1788756, Length=407, Percent_Identity=30.7125307125307, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1789193, Length=393, Percent_Identity=26.4631043256997, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI48994992, Length=376, Percent_Identity=26.3297872340426, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788726, Length=336, Percent_Identity=29.7619047619048, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17564524, Length=323, Percent_Identity=30.030959752322, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 83317; Mature: 83317

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITWLKDLRNEDVDIAGGKGASLGEMWNAKLPVPPAFVVTSDAYKYFIEETKLKDEIK
CCHHHHHHHHCCCCCEECCCCCCCHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
RVLSNIDVNNTEELISASKEVRGAIEKVKLPDDLKISVIEAYNTLCEMGGEEESFVAVRS
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEC
SATAEDLPDASFAGQQETFLNMKGNEQVLKAVQECFSSLFTPRAIFYREEKGFDHFQVAL
CCCHHCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AAVVQKMINADQAGVMFTVNPINHDYNQMVIEGAWGLGEGVVSGTVSPDTYIIEKNTEKI
HHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCEEEEECCCCEE
VDINVARKNTMFIKEDGVTKEVETPEDLKEKQVLTEELILNLQKVGKTIENHYKMPMDIE
EEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
WAIKNGEIYMLQARPITTLGGKKEVKQSLDQDGKILLKGIGASPGIDFGKVKIISEIEQI
EEEECCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
GKILEGDVLVTKMTTPDMVPAMKRASAIVTDDGGLTCHAAIISRELGTPCVVGTKEATKV
HHHHCCCEEEEECCCCCHHHHHHHCCEEEECCCCCEEEHHHHHHHCCCCEEECCHHHHHH
LKDNSCVTVDGEKGMVYDGKIGKKEENIQTQVIYAGSQVITATEIKANVSMPEVAERAAA
HCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEECCEEEEEEEEECCCCCHHHHHHHHC
TGADGVGLLRAEHMILGTGVHPGKILKDKGEDALIEVFSEGIRKVADAFYPRPVTYRTLD
CCCCCCCHHEECEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
APTDEFVGLKGGEDEPHEHNPMMGWRGIRRGLDQPEILKCELKAIKKLREEGYRNINVMI
CCCHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
PLVTNVDEIRKVKEISNEVGLKLMQDVEFGVMIETPAAALIIDEIIKEGIDFASLGTNDL
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCEECCCCCCC
TQYTIAIDRNNENVSKYYLENHPAVMKLIELVITSCKSAGVKTSICGQAGSRPQIVEKLV
EEEEEEEECCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHH
EWGITSISANIDAVETIRNVVARTEQKIILEAIRKQK
HCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLITWLKDLRNEDVDIAGGKGASLGEMWNAKLPVPPAFVVTSDAYKYFIEETKLKDEIK
CCHHHHHHHHCCCCCEECCCCCCCHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
RVLSNIDVNNTEELISASKEVRGAIEKVKLPDDLKISVIEAYNTLCEMGGEEESFVAVRS
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEEC
SATAEDLPDASFAGQQETFLNMKGNEQVLKAVQECFSSLFTPRAIFYREEKGFDHFQVAL
CCCHHCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AAVVQKMINADQAGVMFTVNPINHDYNQMVIEGAWGLGEGVVSGTVSPDTYIIEKNTEKI
HHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCEEEEECCCCEE
VDINVARKNTMFIKEDGVTKEVETPEDLKEKQVLTEELILNLQKVGKTIENHYKMPMDIE
EEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
WAIKNGEIYMLQARPITTLGGKKEVKQSLDQDGKILLKGIGASPGIDFGKVKIISEIEQI
EEEECCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
GKILEGDVLVTKMTTPDMVPAMKRASAIVTDDGGLTCHAAIISRELGTPCVVGTKEATKV
HHHHCCCEEEEECCCCCHHHHHHHCCEEEECCCCCEEEHHHHHHHCCCCEEECCHHHHHH
LKDNSCVTVDGEKGMVYDGKIGKKEENIQTQVIYAGSQVITATEIKANVSMPEVAERAAA
HCCCCEEEEECCCCEEECCCCCCCCCCCEEEEEEECCEEEEEEEEECCCCCHHHHHHHHC
TGADGVGLLRAEHMILGTGVHPGKILKDKGEDALIEVFSEGIRKVADAFYPRPVTYRTLD
CCCCCCCHHEECEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
APTDEFVGLKGGEDEPHEHNPMMGWRGIRRGLDQPEILKCELKAIKKLREEGYRNINVMI
CCCHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
PLVTNVDEIRKVKEISNEVGLKLMQDVEFGVMIETPAAALIIDEIIKEGIDFASLGTNDL
EEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCCEECCCCCCC
TQYTIAIDRNNENVSKYYLENHPAVMKLIELVITSCKSAGVKTSICGQAGSRPQIVEKLV
EEEEEEEECCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHH
EWGITSISANIDAVETIRNVVARTEQKIILEAIRKQK
HCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]