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The map label for this gene is ysaA [C]

Identifier: 150399059

GI number: 150399059

Start: 355406

End: 355903

Strand: Reverse

Name: ysaA [C]

Synonym: Mevan_0305

Alternate gene names: 150399059

Gene position: 355903-355406 (Counterclockwise)

Preceding gene: 150399060

Following gene: 150399058

Centisome position: 20.69

GC content: 35.14

Gene sequence:

>498_bases
ATGAAATCTTTAATGGTAATCGATGCAAGAAAGTGTACAGAATGTAATGATTGTATATCCGCATGTAAAAAAGAACACGG
GGAATCTAGGGCGATTAAAACACACTCTGTCCCAATGTTTTGCATGCACTGCCACCCTGATAAAGCACCATGTAGGCAAG
TATGTCCTGTAGATGCTATCGAAGAAATGGAAGGCGTACTTATTGTAAATGAAGATGCCTGTATTTTGTGCAGATTGTGC
ATGATTGCATGTCCGATTGGGGCACTAGTAATAAACAACGAAAGTAAATCGGTTGAAAAATGTACTTTATGTATGGAAAC
TGATAAAATGCTCCCTGCATGTGTTGAGGCATGTAAATATAATGTCTTAAAGCTCTTTTCAGCAGAAGACTTAGAAGAAC
TTAGGCGGGAAAACTCAAACATGAGTATAATCCTTGAATCTTTAAAAAGGTATAAAGATAAAGATATTCGTAAAATTAAT
GATAATAATGATAAATAA

Upstream 100 bases:

>100_bases
TTTCAGGTGAGATTGAAGATGAATCAGGATGGGAAGTCATTGTAGGTACTAGAGATTCATCAGAAATTCCTGCTTTCCTA
GCAAAAATATGGTGATTTTC

Downstream 100 bases:

>100_bases
TTCTTTTTTAACTTCTTTTAATCATGGTAAAATTGAAAAATACAAAAATGATTTAAACCTTAAACTAATAATAAAAAAAT
AAAAAGATAATGGTAGTATC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKSLMVIDARKCTECNDCISACKKEHGESRAIKTHSVPMFCMHCHPDKAPCRQVCPVDAIEEMEGVLIVNEDACILCRLC
MIACPIGALVINNESKSVEKCTLCMETDKMLPACVEACKYNVLKLFSAEDLEELRRENSNMSIILESLKRYKDKDIRKIN
DNNDK

Sequences:

>Translated_165_residues
MKSLMVIDARKCTECNDCISACKKEHGESRAIKTHSVPMFCMHCHPDKAPCRQVCPVDAIEEMEGVLIVNEDACILCRLC
MIACPIGALVINNESKSVEKCTLCMETDKMLPACVEACKYNVLKLFSAEDLEELRRENSNMSIILESLKRYKDKDIRKIN
DNNDK
>Mature_165_residues
MKSLMVIDARKCTECNDCISACKKEHGESRAIKTHSVPMFCMHCHPDKAPCRQVCPVDAIEEMEGVLIVNEDACILCRLC
MIACPIGALVINNESKSVEKCTLCMETDKMLPACVEACKYNVLKLFSAEDLEELRRENSNMSIILESLKRYKDKDIRKIN
DNNDK

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=154, Percent_Identity=35.0649350649351, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=27.3684210526316, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=176, Percent_Identity=30.6818181818182, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082180, Length=150, Percent_Identity=28.6666666666667, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082179, Length=155, Percent_Identity=30.3225806451613, Blast_Score=73, Evalue=8e-15,
Organism=Escherichia coli, GI1787872, Length=167, Percent_Identity=29.940119760479, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788811, Length=149, Percent_Identity=28.1879194630872, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=26.5957446808511, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1787122, Length=167, Percent_Identity=28.1437125748503, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI226510944, Length=156, Percent_Identity=30.1282051282051, Blast_Score=66, Evalue=9e-13,
Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 18664; Mature: 18664

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
15.2 %Cys+Met (Translated Protein)
9.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
15.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLMVIDARKCTECNDCISACKKEHGESRAIKTHSVPMFCMHCHPDKAPCRQVCPVDAI
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEECCCCEEEEECCCCCCCHHHCCCCHHH
EEMEGVLIVNEDACILCRLCMIACPIGALVINNESKSVEKCTLCMETDKMLPACVEACKY
HHCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NVLKLFSAEDLEELRRENSNMSIILESLKRYKDKDIRKINDNNDK
HHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSLMVIDARKCTECNDCISACKKEHGESRAIKTHSVPMFCMHCHPDKAPCRQVCPVDAI
CCCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEECCCCEEEEECCCCCCCHHHCCCCHHH
EEMEGVLIVNEDACILCRLCMIACPIGALVINNESKSVEKCTLCMETDKMLPACVEACKY
HHCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NVLKLFSAEDLEELRRENSNMSIILESLKRYKDKDIRKINDNNDK
HHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]