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The map label for this gene is vhcA [H]

Identifier: 150398829

GI number: 150398829

Start: 69886

End: 71301

Strand: Reverse

Name: vhcA [H]

Synonym: Mevan_0070

Alternate gene names: 150398829

Gene position: 71301-69886 (Counterclockwise)

Preceding gene: 150398830

Following gene: 150398828

Centisome position: 4.15

GC content: 36.51

Gene sequence:

>1416_bases
ATGACAAAACTGTCAGTAGAACCTGTATCACGGGTTGAAGGACACGGTAAGGTTACACTTTCATTTGATGACAGTGGGAA
GCTTGAAAAAGTTAATTTTCACGTTGTGGAAGTACGAGGATTTGAAAAATTTTTAGAAGGAAGATACATTGAAGATGCCC
CAGTATTTACGCCAAGAATTTGTGGAATTTGCCAGGTTTCACACAATTTAGCAAGTGCAAAAGCAGTAGATAATGTATTT
GGGGTAAAAATTCCAAAAACTGCAAATATGCTCAGAAATTTAATGCAACAAGCTTCAAATGTACACAGCCATGCGCTACA
CTTTGGAATGCTTGCATCTCCAGATTTAATGTTTCCAATGACTGATGACGCATTAAAAAGAAACGTTTTAGGTGTCGCTT
CAGAAAACATTGAGGTTGTAAAAGATGCAATTGCAATGAGAAAAATCGGGCAAACTATTATACAAAAAGTTGGTGGAAGA
GCAATTCACCCAGTAACTGCAATCCCAGGTGGACAATCAAAACCATTAACTGAAAAAGAAAGAGAAGAACTTTTAAAAAT
GTCTGAAAATTTAGTAGAAACTGCTGAAAAGACATTAACTCTCGGAAAACAGTTAGTTGAAGGATTAAAAGAAAAAGACT
TATTTGACATCGGATACTTTGAGTCATACCACATGGGTTTAGTAAATAATGGTGCACAAGACATTTACGAAGGAAATATC
CGTGTTGTAAACCCTGAAGGAAAAATTGAATACGAATACGACCCTTCAGAATATGCAAAATACATGTCAGAAGGTGTAAG
GCCTTATTCATACCTCAAATTCCCGTACCTCACTGAAAAAGGGCCTGTTGATGGAGTTTATAGGGTAAATACCCTTTCAA
GATTAAATGTTTGCGACAAAATGCCAACACCTATTGCTCAAAAATACTACGAAGAATTTGTAAAAACATATGGAAAACCT
GCACATCATCCAATGCTTTTCCACTACGCAAGATTAATAGAATTGGTTTCAAGTGCCGAACTTGTTAGAAAATTCTTAGA
AGATGATACAATTGTTGGAACCGACATAAGGGTTGAACCGACCGATATCGTTGGAGAAGGTGTTGGATGCGTAGAAGCTC
CAAGAGGAACATTGGTACACCACTTCAAAACCGATAATAGAGGTATAATTACGGATGCAAATTTAGTTGTGGCAACCGTT
CAAAATAACCCTGCAATGGACATCGGAGTTCAAAAAGTTGCTCAAAAATTCATAAAATCCCCAGAAGATGCAAAACCACA
CGTTTTAAACTACATGGAAATGGTAATTAGGGCATACGACCCATGTCTTTCATGTGCAACCCATACAATTGACGGAAAAG
AAAGAATGCTTTCAATTGATGTATTAAAAGGCGGAAAATTAATTAAAACATTATAG

Upstream 100 bases:

>100_bases
ACAAATAATAGATAAAATAGGTAGTTTTTATAAATTTACACTCCCAAGTGCTTTAATTCCAATAAATAACAAAAGATAAT
ATCATAAATACGGTGATATG

Downstream 100 bases:

>100_bases
GGGATATTATGATCAAGATAAACACTGAAGATTGTTATTTGTGTAAAGCTTGTGAAAATAACTGCCCTACGGAAGCTTTA
AAATTAAATCCTCTTAAAGT

Product: nickel-dependent hydrogenase large subunit

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTKLSVEPVSRVEGHGKVTLSFDDSGKLEKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRICGICQVSHNLASAKAVDNVF
GVKIPKTANMLRNLMQQASNVHSHALHFGMLASPDLMFPMTDDALKRNVLGVASENIEVVKDAIAMRKIGQTIIQKVGGR
AIHPVTAIPGGQSKPLTEKEREELLKMSENLVETAEKTLTLGKQLVEGLKEKDLFDIGYFESYHMGLVNNGAQDIYEGNI
RVVNPEGKIEYEYDPSEYAKYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDKMPTPIAQKYYEEFVKTYGKP
AHHPMLFHYARLIELVSSAELVRKFLEDDTIVGTDIRVEPTDIVGEGVGCVEAPRGTLVHHFKTDNRGIITDANLVVATV
QNNPAMDIGVQKVAQKFIKSPEDAKPHVLNYMEMVIRAYDPCLSCATHTIDGKERMLSIDVLKGGKLIKTL

Sequences:

>Translated_471_residues
MTKLSVEPVSRVEGHGKVTLSFDDSGKLEKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRICGICQVSHNLASAKAVDNVF
GVKIPKTANMLRNLMQQASNVHSHALHFGMLASPDLMFPMTDDALKRNVLGVASENIEVVKDAIAMRKIGQTIIQKVGGR
AIHPVTAIPGGQSKPLTEKEREELLKMSENLVETAEKTLTLGKQLVEGLKEKDLFDIGYFESYHMGLVNNGAQDIYEGNI
RVVNPEGKIEYEYDPSEYAKYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDKMPTPIAQKYYEEFVKTYGKP
AHHPMLFHYARLIELVSSAELVRKFLEDDTIVGTDIRVEPTDIVGEGVGCVEAPRGTLVHHFKTDNRGIITDANLVVATV
QNNPAMDIGVQKVAQKFIKSPEDAKPHVLNYMEMVIRAYDPCLSCATHTIDGKERMLSIDVLKGGKLIKTL
>Mature_470_residues
TKLSVEPVSRVEGHGKVTLSFDDSGKLEKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRICGICQVSHNLASAKAVDNVFG
VKIPKTANMLRNLMQQASNVHSHALHFGMLASPDLMFPMTDDALKRNVLGVASENIEVVKDAIAMRKIGQTIIQKVGGRA
IHPVTAIPGGQSKPLTEKEREELLKMSENLVETAEKTLTLGKQLVEGLKEKDLFDIGYFESYHMGLVNNGAQDIYEGNIR
VVNPEGKIEYEYDPSEYAKYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDKMPTPIAQKYYEEFVKTYGKPA
HHPMLFHYARLIELVSSAELVRKFLEDDTIVGTDIRVEPTDIVGEGVGCVEAPRGTLVHHFKTDNRGIITDANLVVATVQ
NNPAMDIGVQKVAQKFIKSPEDAKPHVLNYMEMVIRAYDPCLSCATHTIDGKERMLSIDVLKGGKLIKTL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=124, Percent_Identity=37.0967741935484, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 52499; Mature: 52368

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSVEPVSRVEGHGKVTLSFDDSGKLEKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRI
CCCCCCCHHHHCCCCCEEEEEECCCCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCHHH
CGICQVSHNLASAKAVDNVFGVKIPKTANMLRNLMQQASNVHSHALHFGMLASPDLMFPM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
TDDALKRNVLGVASENIEVVKDAIAMRKIGQTIIQKVGGRAIHPVTAIPGGQSKPLTEKE
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCEEECCCCCCCCCCHHH
REELLKMSENLVETAEKTLTLGKQLVEGLKEKDLFDIGYFESYHMGLVNNGAQDIYEGNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCHHCCCCHHHCCCCE
RVVNPEGKIEYEYDPSEYAKYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDK
EEECCCCCEEEEECHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHHC
MPTPIAQKYYEEFVKTYGKPAHHPMLFHYARLIELVSSAELVRKFLEDDTIVGTDIRVEP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEECC
TDIVGEGVGCVEAPRGTLVHHFKTDNRGIITDANLVVATVQNNPAMDIGVQKVAQKFIKS
HHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHCC
PEDAKPHVLNYMEMVIRAYDPCLSCATHTIDGKERMLSIDVLKGGKLIKTL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCHHHCC
>Mature Secondary Structure 
TKLSVEPVSRVEGHGKVTLSFDDSGKLEKVNFHVVEVRGFEKFLEGRYIEDAPVFTPRI
CCCCCCHHHHCCCCCEEEEEECCCCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCHHH
CGICQVSHNLASAKAVDNVFGVKIPKTANMLRNLMQQASNVHSHALHFGMLASPDLMFPM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCC
TDDALKRNVLGVASENIEVVKDAIAMRKIGQTIIQKVGGRAIHPVTAIPGGQSKPLTEKE
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCEEECCCCCCCCCCHHH
REELLKMSENLVETAEKTLTLGKQLVEGLKEKDLFDIGYFESYHMGLVNNGAQDIYEGNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCHHCCCCHHHCCCCE
RVVNPEGKIEYEYDPSEYAKYMSEGVRPYSYLKFPYLTEKGPVDGVYRVNTLSRLNVCDK
EEECCCCCEEEEECHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEECHHHHHHHHHC
MPTPIAQKYYEEFVKTYGKPAHHPMLFHYARLIELVSSAELVRKFLEDDTIVGTDIRVEP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEECC
TDIVGEGVGCVEAPRGTLVHHFKTDNRGIITDANLVVATVQNNPAMDIGVQKVAQKFIKS
HHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHCC
PEDAKPHVLNYMEMVIRAYDPCLSCATHTIDGKERMLSIDVLKGGKLIKTL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]