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The map label for this gene is nifH [H]

Identifier: 150398824

GI number: 150398824

Start: 63631

End: 64458

Strand: Reverse

Name: nifH [H]

Synonym: Mevan_0065

Alternate gene names: 150398824

Gene position: 64458-63631 (Counterclockwise)

Preceding gene: 150398826

Following gene: 150398823

Centisome position: 3.75

GC content: 41.79

Gene sequence:

>828_bases
ATGGTAAGAAAAATCGCAATTTATGGAAAAGGTGGGATTGGGAAGTCAACAACCACCCAAAATACGGTAGCAGCAATGGC
ATATTTTCACGATAAAAAGGTATTTATCCACGGCTGTGACCCGAAAGCAGACAGTACAAGGTTAATCTTGCACGGAAAAC
AACAAACAACAATGATGGACACATTAAGGGAAAAAGGGGAAGACGAATGTACTCCTGATAAAGTTATTGAAACCGGATTT
TGTGGAGTCAGGTGTGTAGAATCAGGTGGGCCGGAACCAGGTGTGGGGTGTGCAGGTAGAGGCGTTATAACCGCAATTAC
ACTCATGGAACAACACGGTGTTTACGAAGACAACCTTGACTTCGTATTCTTCGATGTTTTAGGGGACGTTGTATGCGGTG
GATTTGCAATGCCTGTAAGGGACGGTAAAGCAGACGAAATCTACGTTGTAGCATCGGGAGAAATGATGGCTCTTTACGCT
GCAAACAACATCTGTAAGGGAATGGTTAAATACGCACAACAAAGCGGTGTAAGACTCGGTGGTATCATCTGTAACAGCAG
AAACGTGGATGGTGAACTTGAATTATTACAAGAATTCTGTGATAAACTCGGAACACAATTAATCCACTTCGTTCCAAGGG
ACAACATCGTTCAAAAAGCAGAGTTCCAGAAAAAAGCCGTAGTTGACTTCAACGACACATGCAACCAAGCAATGGAATAC
AAAGAACTCGCAAGAAAAATCATCGAGAACAAAAACCTCGTAATCCCAACACCAATGACAATGGACGAATTAGAAGAATT
AACTTCAAAATACGGTTTCTTAGATTAA

Upstream 100 bases:

>100_bases
GGAATATAGTATATGATATTTCACCGGAAAGTTACTTCCGTTAAATAATAAATATAAGGAATTAAAATTCCTAAAATGAT
GATGCAATAGAGGCTTTAGT

Downstream 100 bases:

>100_bases
TTAAAATAAATTACTTACTTTAAATTTAAAGAGGGCAAAAAATGAAAATGATTAGAGCAATTGTTAGGCCAAGTAAAGCA
GAAGAAGTAGTTGACAAACT

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MVRKIAIYGKGGIGKSTTTQNTVAAMAYFHDKKVFIHGCDPKADSTRLILHGKQQTTMMDTLREKGEDECTPDKVIETGF
CGVRCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDNLDFVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYA
ANNICKGMVKYAQQSGVRLGGIICNSRNVDGELELLQEFCDKLGTQLIHFVPRDNIVQKAEFQKKAVVDFNDTCNQAMEY
KELARKIIENKNLVIPTPMTMDELEELTSKYGFLD

Sequences:

>Translated_275_residues
MVRKIAIYGKGGIGKSTTTQNTVAAMAYFHDKKVFIHGCDPKADSTRLILHGKQQTTMMDTLREKGEDECTPDKVIETGF
CGVRCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDNLDFVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYA
ANNICKGMVKYAQQSGVRLGGIICNSRNVDGELELLQEFCDKLGTQLIHFVPRDNIVQKAEFQKKAVVDFNDTCNQAMEY
KELARKIIENKNLVIPTPMTMDELEELTSKYGFLD
>Mature_275_residues
MVRKIAIYGKGGIGKSTTTQNTVAAMAYFHDKKVFIHGCDPKADSTRLILHGKQQTTMMDTLREKGEDECTPDKVIETGF
CGVRCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDNLDFVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYA
ANNICKGMVKYAQQSGVRLGGIICNSRNVDGELELLQEFCDKLGTQLIHFVPRDNIVQKAEFQKKAVVDFNDTCNQAMEY
KELARKIIENKNLVIPTPMTMDELEELTSKYGFLD

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 30262; Mature: 30262

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKIAIYGKGGIGKSTTTQNTVAAMAYFHDKKVFIHGCDPKADSTRLILHGKQQTTMMD
CCEEEEEEECCCCCCCCCCHHHHHHHHEEECCEEEEECCCCCCCCCEEEEECCCHHHHHH
TLREKGEDECTPDKVIETGFCGVRCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDNLD
HHHHCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
FVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYAANNICKGMVKYAQQSGVRLG
EEEHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCEEE
GIICNSRNVDGELELLQEFCDKLGTQLIHFVPRDNIVQKAEFQKKAVVDFNDTCNQAMEY
EEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHEEECCCHHHHHHHHH
KELARKIIENKNLVIPTPMTMDELEELTSKYGFLD
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVRKIAIYGKGGIGKSTTTQNTVAAMAYFHDKKVFIHGCDPKADSTRLILHGKQQTTMMD
CCEEEEEEECCCCCCCCCCHHHHHHHHEEECCEEEEECCCCCCCCCEEEEECCCHHHHHH
TLREKGEDECTPDKVIETGFCGVRCVESGGPEPGVGCAGRGVITAITLMEQHGVYEDNLD
HHHHCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
FVFFDVLGDVVCGGFAMPVRDGKADEIYVVASGEMMALYAANNICKGMVKYAQQSGVRLG
EEEHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHHCCCEEE
GIICNSRNVDGELELLQEFCDKLGTQLIHFVPRDNIVQKAEFQKKAVVDFNDTCNQAMEY
EEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHEEECCCHHHHHHHHH
KELARKIIENKNLVIPTPMTMDELEELTSKYGFLD
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592322 [H]