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The map label for this gene is dut

Identifier: 150398737

GI number: 150398737

Start: 3759269

End: 3759751

Strand: Reverse

Name: dut

Synonym: Smed_3554

Alternate gene names: 150398737

Gene position: 3759751-3759269 (Counterclockwise)

Preceding gene: 150398739

Following gene: 150398735

Centisome position: 99.41

GC content: 66.25

Gene sequence:

>483_bases
ATGCACAGACCCGAAAATCGCGCTGATCTCACCCCTGACCTCACCCTCGTCCGCCTGTCCCATGGCGAAGGCCTCGACCT
GCCTGCCTACGAGACCGCGGGTGCCGCCGGTATGGACCTCCGCGCGGCCGTCCCGGCAGATGAGCCGATGACGATCCGGC
CGGGCGCGCGCACGCTGGTCCCGACGGGCTTCGTCTTCGAGATTCCGGCTGGTCATGAAGGACAGATTCGTCCGCGCTCC
GGCCTTGCCTTCAAGCACGGCATTACCTGCCTCAACACGCCGGGCACCGTCGACAGCGATTATCGCGGCGAAGTAAAGGT
GCTGCTCGTCAATCTCGGCTCGGATGATTTCGTTGTCGAGCGCGGCATGCGTATCGCCCAGATGGTGATCGCCCCGGTCA
CCCAAATGGCGATCCGCGAGGCAGACGGCGCGTCCACAACCGCGCGCGGCGGCGGCGGCTTCGGCTCGACGGGCCTCAGT
TAA

Upstream 100 bases:

>100_bases
ATGATCTGAGTATCCTGTGAGTTGCGGCGTCCTTTACAGGCTCGGGGGCCAATATGCAATTGACTGCGGCAAGCCGGGCC
GTGCTTATGAAGGAATGAGC

Downstream 100 bases:

>100_bases
GGGGCGGCAAGCGCCGCTTGACCGGCGACGATTTGACGATCGACGTATTCGTCTGTGCCTTTTCCGCGATGCGATCGAGA
ATCGTGTCGAGTTCCGCCAT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MHRPENRADLTPDLTLVRLSHGEGLDLPAYETAGAAGMDLRAAVPADEPMTIRPGARTLVPTGFVFEIPAGHEGQIRPRS
GLAFKHGITCLNTPGTVDSDYRGEVKVLLVNLGSDDFVVERGMRIAQMVIAPVTQMAIREADGASTTARGGGGFGSTGLS

Sequences:

>Translated_160_residues
MHRPENRADLTPDLTLVRLSHGEGLDLPAYETAGAAGMDLRAAVPADEPMTIRPGARTLVPTGFVFEIPAGHEGQIRPRS
GLAFKHGITCLNTPGTVDSDYRGEVKVLLVNLGSDDFVVERGMRIAQMVIAPVTQMAIREADGASTTARGGGGFGSTGLS
>Mature_160_residues
MHRPENRADLTPDLTLVRLSHGEGLDLPAYETAGAAGMDLRAAVPADEPMTIRPGARTLVPTGFVFEIPAGHEGQIRPRS
GLAFKHGITCLNTPGTVDSDYRGEVKVLLVNLGSDDFVVERGMRIAQMVIAPVTQMAIREADGASTTARGGGGFGSTGLS

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=41.3793103448276, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=41.3793103448276, Blast_Score=92, Evalue=1e-19,
Organism=Homo sapiens, GI70906441, Length=146, Percent_Identity=41.0958904109589, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1790071, Length=138, Percent_Identity=42.0289855072464, Blast_Score=107, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71988561, Length=145, Percent_Identity=42.0689655172414, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319729, Length=133, Percent_Identity=42.1052631578947, Blast_Score=92, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=38.3458646616541, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921126, Length=133, Percent_Identity=38.3458646616541, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_SINMW (A6UFD9)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001329204.1
- ProteinModelPortal:   A6UFD9
- SMR:   A6UFD9
- STRING:   A6UFD9
- GeneID:   5324442
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3554
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   SMED366394:SMED_3554-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16810; Mature: 16810

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: BINDING 92-92 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRPENRADLTPDLTLVRLSHGEGLDLPAYETAGAAGMDLRAAVPADEPMTIRPGARTLV
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCEEE
PTGFVFEIPAGHEGQIRPRSGLAFKHGITCLNTPGTVDSDYRGEVKVLLVNLGSDDFVVE
CCCEEEECCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCEEEEEEEECCCCCHHHH
RGMRIAQMVIAPVTQMAIREADGASTTARGGGGFGSTGLS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHRPENRADLTPDLTLVRLSHGEGLDLPAYETAGAAGMDLRAAVPADEPMTIRPGARTLV
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCEEE
PTGFVFEIPAGHEGQIRPRSGLAFKHGITCLNTPGTVDSDYRGEVKVLLVNLGSDDFVVE
CCCEEEECCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCEEEEEEEECCCCCHHHH
RGMRIAQMVIAPVTQMAIREADGASTTARGGGGFGSTGLS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA