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The map label for this gene is 150398602

Identifier: 150398602

GI number: 150398602

Start: 3620288

End: 3621649

Strand: Reverse

Name: 150398602

Synonym: Smed_3413

Alternate gene names: NA

Gene position: 3621649-3620288 (Counterclockwise)

Preceding gene: 150398605

Following gene: 150398601

Centisome position: 95.76

GC content: 66.45

Gene sequence:

>1362_bases
ATGACCAATCAGCAAACACTCGCGAGCCTCGCGGTTCGCGTTCAGTCCGGGCAACTCGATCCCATCACCCTCGTCGAGGA
AACGCTAACGGAGATCGGGAGCCATCCCGACAAATCGATCTTCGTCGCGCTCACGCGGGAGCGCGCAACACGGGAAGCCC
AGGCCGCCTCCGAGCGCATGAGGGCCGGCCGGTCCCTCGGACTGCTCGACGGCCTGCCGGTCGCCTGGAAGGACCTCTTC
GATCTTGCCGGCAGCGTCACCACGGCGGGTTCCGCCGTGCTCGAAAACAGGTCCCGGGCCGTTACCGACGCGGCGGTGGT
CGCAAAGCTCGCAAGCGCCGGCATGGTCAGCATCGGGCGGACCAATTTGAGCGAGTTCGCCTTTTCGGGGCTTGGCATCA
ACCCGCATTACGGTACGCCGCGCAACCCGCGTTCCACGGATGCCCACCGCATCCCGGGCGGCTCGTCCTCCGGCTCCGCC
GCGGCTGTCGCCGCCGGCCTCGTGCCGCTGGCGATCGGTACCGATACCGGCGGCTCGGTCCGCATCCCGGCGGCAATGAA
CGGCATCGTCGGCTACAAGGCGACACGCGGGCGCTACGCGATGAGAGGCGTCTTTCCGCTTGCCCGCAGTCTGGATTCGC
TCGGCCCGCTTTGCCGGACGGTGCAGGATGCGGTCTGGGCAGACGCGGCAATGCATGGACTGACCGCACCGGTGATCCGC
CGTGCCGAGCTCGCCGATCTTTCGATCGTCATTGCCGATACGATCGTCTTCGACGATGCCGAGCCGGAGATCGTGACGGC
CTTCGAAGGAGCGATCAAGCGGCTCGAGGCAGCCGGCGTAAAAATCCGGCGGCAGGCCTTTCCGAGCTTTGCCGAAATTT
TCGAGCTCACCGCCCGCCACGGCGCATTGGTGACGGCGGAGGCTTATGCGCTGCATCGGGAGCGCCTCGCAGGGGCCGAG
GCCGCCAACATGGATCCGCGCGTCGTCGCCCGCACCCGCCTCGGCGAGAAGATAACCGTCAGCGACTATATCGCTCTCCT
CGAAGCGCGCGACCGCTTGATCCATGAGGCGGGCGAGAGCATCTCGGCCGGCGAGTTGATCGCGCATCCGACCCTGCCGC
ATGTCGCGCCGCTGCTCGATCCGCTGCTTGCCGACTACGATCTCTTCTTCAAGACAAACGCCAGGACGCTGCGCAATACC
TCGATCGGAAACTTCCTGGATTTCTGTGGTGTCTCCATCCCGTGCGGCACGGGTGGGGCCGGCATGCCGGTCGGCTTCCA
GCTTTCGGCACCTCACCACCAGGACGACCGCCTGCTATCCGCAGCGCTCGCCGCCGAGGCGATCATCCGGGGCGATGCAT
GA

Upstream 100 bases:

>100_bases
GTCGCTGGAGGAGCCTTGCTCTATTGCATTGCTGGAAAGCAGGGCACTGTCGTGTACGGTCGGCGGATGCGATATGCTGG
AGCGGGATGAGGAAGGCGCC

Downstream 100 bases:

>100_bases
TCGTCTGCTTCGACATCGGCGGCTCGGCGATCAAGGGCGCCATCACCCATTCGCCGGACCGGATATTTCCCTTGCCCCGG
CGCGTGACGCCGCTCACCGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTNQQTLASLAVRVQSGQLDPITLVEETLTEIGSHPDKSIFVALTRERATREAQAASERMRAGRSLGLLDGLPVAWKDLF
DLAGSVTTAGSAVLENRSRAVTDAAVVAKLASAGMVSIGRTNLSEFAFSGLGINPHYGTPRNPRSTDAHRIPGGSSSGSA
AAVAAGLVPLAIGTDTGGSVRIPAAMNGIVGYKATRGRYAMRGVFPLARSLDSLGPLCRTVQDAVWADAAMHGLTAPVIR
RAELADLSIVIADTIVFDDAEPEIVTAFEGAIKRLEAAGVKIRRQAFPSFAEIFELTARHGALVTAEAYALHRERLAGAE
AANMDPRVVARTRLGEKITVSDYIALLEARDRLIHEAGESISAGELIAHPTLPHVAPLLDPLLADYDLFFKTNARTLRNT
SIGNFLDFCGVSIPCGTGGAGMPVGFQLSAPHHQDDRLLSAALAAEAIIRGDA

Sequences:

>Translated_453_residues
MTNQQTLASLAVRVQSGQLDPITLVEETLTEIGSHPDKSIFVALTRERATREAQAASERMRAGRSLGLLDGLPVAWKDLF
DLAGSVTTAGSAVLENRSRAVTDAAVVAKLASAGMVSIGRTNLSEFAFSGLGINPHYGTPRNPRSTDAHRIPGGSSSGSA
AAVAAGLVPLAIGTDTGGSVRIPAAMNGIVGYKATRGRYAMRGVFPLARSLDSLGPLCRTVQDAVWADAAMHGLTAPVIR
RAELADLSIVIADTIVFDDAEPEIVTAFEGAIKRLEAAGVKIRRQAFPSFAEIFELTARHGALVTAEAYALHRERLAGAE
AANMDPRVVARTRLGEKITVSDYIALLEARDRLIHEAGESISAGELIAHPTLPHVAPLLDPLLADYDLFFKTNARTLRNT
SIGNFLDFCGVSIPCGTGGAGMPVGFQLSAPHHQDDRLLSAALAAEAIIRGDA
>Mature_452_residues
TNQQTLASLAVRVQSGQLDPITLVEETLTEIGSHPDKSIFVALTRERATREAQAASERMRAGRSLGLLDGLPVAWKDLFD
LAGSVTTAGSAVLENRSRAVTDAAVVAKLASAGMVSIGRTNLSEFAFSGLGINPHYGTPRNPRSTDAHRIPGGSSSGSAA
AVAAGLVPLAIGTDTGGSVRIPAAMNGIVGYKATRGRYAMRGVFPLARSLDSLGPLCRTVQDAVWADAAMHGLTAPVIRR
AELADLSIVIADTIVFDDAEPEIVTAFEGAIKRLEAAGVKIRRQAFPSFAEIFELTARHGALVTAEAYALHRERLAGAEA
ANMDPRVVARTRLGEKITVSDYIALLEARDRLIHEAGESISAGELIAHPTLPHVAPLLDPLLADYDLFFKTNARTLRNTS
IGNFLDFCGVSIPCGTGGAGMPVGFQLSAPHHQDDRLLSAALAAEAIIRGDA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=25.0489236790607, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.6619144602851, Blast_Score=110, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=206, Percent_Identity=36.4077669902913, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17537465, Length=424, Percent_Identity=25.2358490566038, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=159, Percent_Identity=38.3647798742138, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=156, Percent_Identity=34.6153846153846, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=175, Percent_Identity=34.8571428571429, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=231, Percent_Identity=31.6017316017316, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=135, Percent_Identity=40, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=135, Percent_Identity=40, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=385, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=164, Percent_Identity=39.0243902439024, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648113, Length=510, Percent_Identity=29.8039215686275, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=25.1004016064257, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=25.1004016064257, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=25.1004016064257, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=25.1004016064257, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356731, Length=437, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=23.8289205702648, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648435, Length=174, Percent_Identity=31.6091954022989, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648437, Length=174, Percent_Identity=31.6091954022989, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550774, Length=174, Percent_Identity=31.6091954022989, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648441, Length=174, Percent_Identity=31.6091954022989, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648439, Length=174, Percent_Identity=31.6091954022989, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=25.2, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 47679; Mature: 47548

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQQTLASLAVRVQSGQLDPITLVEETLTEIGSHPDKSIFVALTRERATREAQAASERM
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHH
RAGRSLGLLDGLPVAWKDLFDLAGSVTTAGSAVLENRSRAVTDAAVVAKLASAGMVSIGR
HCCCCCCCCCCCCHHHHHHHHHHCCHHHCCHHHHHCCHHHHHHHHHHHHHHHCCEEEECC
TNLSEFAFSGLGINPHYGTPRNPRSTDAHRIPGGSSSGSAAAVAAGLVPLAIGTDTGGSV
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCE
RIPAAMNGIVGYKATRGRYAMRGVFPLARSLDSLGPLCRTVQDAVWADAAMHGLTAPVIR
ECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RAELADLSIVIADTIVFDDAEPEIVTAFEGAIKRLEAAGVKIRRQAFPSFAEIFELTARH
HHHHCCEEEEEEEEEEECCCCCCEEHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHC
GALVTAEAYALHRERLAGAEAANMDPRVVARTRLGEKITVSDYIALLEARDRLIHEAGES
CCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCC
ISAGELIAHPTLPHVAPLLDPLLADYDLFFKTNARTLRNTSIGNFLDFCGVSIPCGTGGA
CCCCCEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHCCCCCCCCCCC
GMPVGFQLSAPHHQDDRLLSAALAAEAIIRGDA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNQQTLASLAVRVQSGQLDPITLVEETLTEIGSHPDKSIFVALTRERATREAQAASERM
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHH
RAGRSLGLLDGLPVAWKDLFDLAGSVTTAGSAVLENRSRAVTDAAVVAKLASAGMVSIGR
HCCCCCCCCCCCCHHHHHHHHHHCCHHHCCHHHHHCCHHHHHHHHHHHHHHHCCEEEECC
TNLSEFAFSGLGINPHYGTPRNPRSTDAHRIPGGSSSGSAAAVAAGLVPLAIGTDTGGSV
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCE
RIPAAMNGIVGYKATRGRYAMRGVFPLARSLDSLGPLCRTVQDAVWADAAMHGLTAPVIR
ECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RAELADLSIVIADTIVFDDAEPEIVTAFEGAIKRLEAAGVKIRRQAFPSFAEIFELTARH
HHHHCCEEEEEEEEEEECCCCCCEEHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHC
GALVTAEAYALHRERLAGAEAANMDPRVVARTRLGEKITVSDYIALLEARDRLIHEAGES
CCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHCCCC
ISAGELIAHPTLPHVAPLLDPLLADYDLFFKTNARTLRNTSIGNFLDFCGVSIPCGTGGA
CCCCCEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHCCCCCCCCCCC
GMPVGFQLSAPHHQDDRLLSAALAAEAIIRGDA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9809753 [H]