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The map label for this gene is ttgG [H]

Identifier: 150398588

GI number: 150398588

Start: 3606549

End: 3607769

Strand: Reverse

Name: ttgG [H]

Synonym: Smed_3399

Alternate gene names: 150398588

Gene position: 3607769-3606549 (Counterclockwise)

Preceding gene: 150398589

Following gene: 150398587

Centisome position: 95.4

GC content: 63.64

Gene sequence:

>1221_bases
ATGGACATTGCGATGCGCATGAACCGACCGATTTTGGCCGCCTCGCTGGCAACCGTGATTTTTCTCGCGGGGTGCCAGAA
GAATGAAGAACAGGCCGCGGCTCCCGCCCCTCCCCCGTCGCCGGTCGCCGTCTTCACGACGAAGGCCGAACCACTTCCGA
TCACCAACGAACTCCCGGGTCGCATCACCGCTACCCGGATTGCCGAGGTCCGCCCGCGCATCTCAGGTATCGTCGTCGAG
CGGGTGTTCGAACAGGGCACCATGGTGAAGGAAGGCGATGTGCTCTACCGCATCGACCCGGCGCCGTTCCAGGTAAAGGT
CGACAGCGCCGAGGCTACGCTGAAGCGCGCCAAGTCCCTGGTCGATCAGGCCAGGCGAACGGCCGACCGCCAATCGCGGC
TGAAGGAAGCGCAGGTTACAGCGGTGCAGCAATACGACGACGCCATAGCGACCCTTGCCCAGGCAGAGGCCGATGTCGGC
ATCGCCGAGGCTGGTCTCGCGGAGGCCAGGCTCAATCTGCAATATACGAACGTCACGGCTCCGATCAGCGGCCGGATCGG
CCGGGCGCTGATCACCGAAGGCGCCCTCGTCAACACCAACGATACGCAGAACCTCGCGACGATCCAACAGCTGGACCCGA
TCTATGCCGACTTCACCCAGTCCGCGACCGACCTGATCCGCCTGCGCAAGGCGCTCAAGGACGGCAAGTGGATGAGCTCC
AAAAACGAAGCCGAGGTGCAGCTGATCCTCGATGACGGCACGCCCTATGCCCTCAAGGGCAAGCTGCTCTTTTCCGAAGC
CGCCGTCGATGCGACGACCGGTCAGGTGACGCTGCGCGGCGAATTCCCCAATCCGAACAATGACCTGCTGCCCGGCATGT
ATGTCCGCGTACAAATCCAGCAAGGTCTCGAAGAGGACGCGATCACCGTTCCGCAACAGGCGGTCCAGCGCAACAATGCC
GGCCAGTCGCAGGTCTATGTCGTGACTGCCGACAACAAGGTGGAGTTCCGCAACGTCACTCTCGGACGCATTGTCAGCCA
GCGCTGGCAGGTGACGTCGGGCCTGAAGCCCGGCGAAAAGGTCATCGTCGAGGGCTTCCAGAAGATCGGCCCGGGCGCAC
CCGTGCAGCCTTCGGATTGGGATCCGAACGCCAAGCCTGCGCCCGAACAGGCCAGCGCGTCGGCAGATGCCGGCAAAAAG
CCCGCCACCGACGCGAAGTGA

Upstream 100 bases:

>100_bases
GCTTTCCGGTATTTTCACGCCGATACCGGCTCCGCTTCACCTCACTTTCGGACCGCGCAGCGCTGCGACAAGCCGCGGCT
GCGGCCGGTCCTTTGACAAT

Downstream 100 bases:

>100_bases
GAATTTTATCATGCCCAGTTTTTTCATAGACAGGCCTATCTTTGCCTGGGTGGTGGCGATCTTCATCATGATCGCCGGCA
TCATCGCGATCCCGCTATTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MDIAMRMNRPILAASLATVIFLAGCQKNEEQAAAPAPPPSPVAVFTTKAEPLPITNELPGRITATRIAEVRPRISGIVVE
RVFEQGTMVKEGDVLYRIDPAPFQVKVDSAEATLKRAKSLVDQARRTADRQSRLKEAQVTAVQQYDDAIATLAQAEADVG
IAEAGLAEARLNLQYTNVTAPISGRIGRALITEGALVNTNDTQNLATIQQLDPIYADFTQSATDLIRLRKALKDGKWMSS
KNEAEVQLILDDGTPYALKGKLLFSEAAVDATTGQVTLRGEFPNPNNDLLPGMYVRVQIQQGLEEDAITVPQQAVQRNNA
GQSQVYVVTADNKVEFRNVTLGRIVSQRWQVTSGLKPGEKVIVEGFQKIGPGAPVQPSDWDPNAKPAPEQASASADAGKK
PATDAK

Sequences:

>Translated_406_residues
MDIAMRMNRPILAASLATVIFLAGCQKNEEQAAAPAPPPSPVAVFTTKAEPLPITNELPGRITATRIAEVRPRISGIVVE
RVFEQGTMVKEGDVLYRIDPAPFQVKVDSAEATLKRAKSLVDQARRTADRQSRLKEAQVTAVQQYDDAIATLAQAEADVG
IAEAGLAEARLNLQYTNVTAPISGRIGRALITEGALVNTNDTQNLATIQQLDPIYADFTQSATDLIRLRKALKDGKWMSS
KNEAEVQLILDDGTPYALKGKLLFSEAAVDATTGQVTLRGEFPNPNNDLLPGMYVRVQIQQGLEEDAITVPQQAVQRNNA
GQSQVYVVTADNKVEFRNVTLGRIVSQRWQVTSGLKPGEKVIVEGFQKIGPGAPVQPSDWDPNAKPAPEQASASADAGKK
PATDAK
>Mature_406_residues
MDIAMRMNRPILAASLATVIFLAGCQKNEEQAAAPAPPPSPVAVFTTKAEPLPITNELPGRITATRIAEVRPRISGIVVE
RVFEQGTMVKEGDVLYRIDPAPFQVKVDSAEATLKRAKSLVDQARRTADRQSRLKEAQVTAVQQYDDAIATLAQAEADVG
IAEAGLAEARLNLQYTNVTAPISGRIGRALITEGALVNTNDTQNLATIQQLDPIYADFTQSATDLIRLRKALKDGKWMSS
KNEAEVQLILDDGTPYALKGKLLFSEAAVDATTGQVTLRGEFPNPNNDLLPGMYVRVQIQQGLEEDAITVPQQAVQRNNA
GQSQVYVVTADNKVEFRNVTLGRIVSQRWQVTSGLKPGEKVIVEGFQKIGPGAPVQPSDWDPNAKPAPEQASASADAGKK
PATDAK

Specific function: The periplasmic linker component of an organic solvent efflux pump. Involved in export of a number of organic solvents, including toluene and styrene. This is the most important solvent efflux pump in this strain, although it can export AMP and some antib

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=389, Percent_Identity=44.987146529563, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1789665, Length=368, Percent_Identity=44.5652173913043, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1789929, Length=369, Percent_Identity=41.1924119241192, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI87082036, Length=371, Percent_Identity=29.3800539083558, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI87081796, Length=326, Percent_Identity=24.2331288343558, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081951, Length=127, Percent_Identity=32.2834645669291, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43769; Mature: 43769

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAMRMNRPILAASLATVIFLAGCQKNEEQAAAPAPPPSPVAVFTTKAEPLPITNELPG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
RITATRIAEVRPRISGIVVERVFEQGTMVKEGDVLYRIDPAPFQVKVDSAEATLKRAKSL
CEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEEECCHHHHHHHHHHH
VDQARRTADRQSRLKEAQVTAVQQYDDAIATLAQAEADVGIAEAGLAEARLNLQYTNVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHEEEEEEEEEEC
PISGRIGRALITEGALVNTNDTQNLATIQQLDPIYADFTQSATDLIRLRKALKDGKWMSS
CCCCCCCHHHHCCCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KNEAEVQLILDDGTPYALKGKLLFSEAAVDATTGQVTLRGEFPNPNNDLLPGMYVRVQIQ
CCCCEEEEEEECCCCEEEEEEEEEEHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEC
QGLEEDAITVPQQAVQRNNAGQSQVYVVTADNKVEFRNVTLGRIVSQRWQVTSGLKPGEK
CCCCCCHHCCCHHHHHCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCHH
VIVEGFQKIGPGAPVQPSDWDPNAKPAPEQASASADAGKKPATDAK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDIAMRMNRPILAASLATVIFLAGCQKNEEQAAAPAPPPSPVAVFTTKAEPLPITNELPG
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
RITATRIAEVRPRISGIVVERVFEQGTMVKEGDVLYRIDPAPFQVKVDSAEATLKRAKSL
CEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEEECCHHHHHHHHHHH
VDQARRTADRQSRLKEAQVTAVQQYDDAIATLAQAEADVGIAEAGLAEARLNLQYTNVTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHEEEEEEEEEEC
PISGRIGRALITEGALVNTNDTQNLATIQQLDPIYADFTQSATDLIRLRKALKDGKWMSS
CCCCCCCHHHHCCCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KNEAEVQLILDDGTPYALKGKLLFSEAAVDATTGQVTLRGEFPNPNNDLLPGMYVRVQIQ
CCCCEEEEEEECCCCEEEEEEEEEEHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEC
QGLEEDAITVPQQAVQRNNAGQSQVYVVTADNKVEFRNVTLGRIVSQRWQVTSGLKPGEK
CCCCCCHHCCCHHHHHCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCHH
VIVEGFQKIGPGAPVQPSDWDPNAKPAPEQASASADAGKKPATDAK
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11395460 [H]