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The map label for this gene is ada [H]

Identifier: 150398562

GI number: 150398562

Start: 3575288

End: 3576160

Strand: Reverse

Name: ada [H]

Synonym: Smed_3373

Alternate gene names: 150398562

Gene position: 3576160-3575288 (Counterclockwise)

Preceding gene: 150398563

Following gene: 150398555

Centisome position: 94.56

GC content: 64.15

Gene sequence:

>873_bases
ATGAACGTTGCTACATCCATTCCCACCGACATCACTCCGGAAGGCACCGACTACGACACGGTGACTCGTGTCATCGCGAT
GCTGACCGAAGACTATCGCGAGCAGCCGTCGCTCGAGTCGCTCGCCCGTCGCCTCGGGCAGTCGCCGACGCAACTTCAGA
AAGTTTTCACCCGTTGGGCGGGGCTCTCACCCAAGGCCTTTCTGCAGGCAATCACTCTCGATCACGCCAAACGGCTCCTG
CGCCAGGAAGACCTGCCGTTGCTGGAGACCAGCATTGAGATCGGCCTGTCCGGGCCGAGCCGGCTGCACGATCTCTTCGT
AACGCATGAGGCGATGTCCCCCGGTGAATGGAAAGCGCGCGGCGCGGGCCTTACCATCCGCTACGGGTTTCACCCTTCAC
CCTTCGGGACGGCGCTGGTCATGGTGACCGAGCGCGGTCTCGCCGGACTGGCCTTCGCCGATTCAGGCGAGGAGCACGCG
AGCTTCGAGGACATGGCCTCCCGCTGGCCGAACGCAATCTACCTTGAAGACAGCGCTGCAACGGCGCGCTATGCGGCGCG
CATTTTCGACCCCGATCGGTGGTCCGCGGAGGAGCCGCTGAGGATTTTTCTGATCGGCTCCGATTTTCAGGTCCGCGTAT
GGCAGACGCTTCTCAAGATTCCGCTCGGTAAGGCAACGACCTATTCGAAAATCGCGGAGAATATCGGCCAGCCAACCGCT
TCGCGCGCCGTCGGCGCCGCGGTGGGGCGCAATCCGATCTCCTTCGTCGTGCCCTGCCACCGGGCGCTCGGCAAGGCCGG
CGATCTCACCGGCTACCATTGGGGGCTGACGCGCAAGCGCGCGATCCTCGGCTGGGAGGCGGGGAAGGCCTGA

Upstream 100 bases:

>100_bases
GCGACCGCCAGGTCCCGATGAATGCCTGCGATCGAGCCCGACAAGAATCGGGTGGTTCCAGGCTCGCGCGCATGATTATC
TGAAGCTCAAGGAGATGATC

Downstream 100 bases:

>100_bases
TGGCCGGGGTGTGGCTGGAGCCGGGCAGCGAACGCCCCCTCATCCGCCTGCCGGCACCTTCTTCCCGCAAGCGGGGGAAG
GGACAAGCGGCGAGCGAGCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNVATSIPTDITPEGTDYDTVTRVIAMLTEDYREQPSLESLARRLGQSPTQLQKVFTRWAGLSPKAFLQAITLDHAKRLL
RQEDLPLLETSIEIGLSGPSRLHDLFVTHEAMSPGEWKARGAGLTIRYGFHPSPFGTALVMVTERGLAGLAFADSGEEHA
SFEDMASRWPNAIYLEDSAATARYAARIFDPDRWSAEEPLRIFLIGSDFQVRVWQTLLKIPLGKATTYSKIAENIGQPTA
SRAVGAAVGRNPISFVVPCHRALGKAGDLTGYHWGLTRKRAILGWEAGKA

Sequences:

>Translated_290_residues
MNVATSIPTDITPEGTDYDTVTRVIAMLTEDYREQPSLESLARRLGQSPTQLQKVFTRWAGLSPKAFLQAITLDHAKRLL
RQEDLPLLETSIEIGLSGPSRLHDLFVTHEAMSPGEWKARGAGLTIRYGFHPSPFGTALVMVTERGLAGLAFADSGEEHA
SFEDMASRWPNAIYLEDSAATARYAARIFDPDRWSAEEPLRIFLIGSDFQVRVWQTLLKIPLGKATTYSKIAENIGQPTA
SRAVGAAVGRNPISFVVPCHRALGKAGDLTGYHWGLTRKRAILGWEAGKA
>Mature_290_residues
MNVATSIPTDITPEGTDYDTVTRVIAMLTEDYREQPSLESLARRLGQSPTQLQKVFTRWAGLSPKAFLQAITLDHAKRLL
RQEDLPLLETSIEIGLSGPSRLHDLFVTHEAMSPGEWKARGAGLTIRYGFHPSPFGTALVMVTERGLAGLAFADSGEEHA
SFEDMASRWPNAIYLEDSAATARYAARIFDPDRWSAEEPLRIFLIGSDFQVRVWQTLLKIPLGKATTYSKIAENIGQPTA
SRAVGAAVGRNPISFVVPCHRALGKAGDLTGYHWGLTRKRAILGWEAGKA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=289, Percent_Identity=28.719723183391, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=53.8461538461538, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=44.4444444444444, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=44.4444444444444, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=47.6190476190476, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137554, Length=174, Percent_Identity=31.6091954022989, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31875; Mature: 31875

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVATSIPTDITPEGTDYDTVTRVIAMLTEDYREQPSLESLARRLGQSPTQLQKVFTRWA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHC
GLSPKAFLQAITLDHAKRLLRQEDLPLLETSIEIGLSGPSRLHDLFVTHEAMSPGEWKAR
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEECCCCHHHHHHHHHHHCCCCCCCCEEC
GAGLTIRYGFHPSPFGTALVMVTERGLAGLAFADSGEEHASFEDMASRWPNAIYLEDSAA
CCCEEEEECCCCCCCCCEEEEEECCCCCEEEECCCCCHHCCHHHHHHHCCCEEEEECCCH
TARYAARIFDPDRWSAEEPLRIFLIGSDFQVRVWQTLLKIPLGKATTYSKIAENIGQPTA
HHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
SRAVGAAVGRNPISFVVPCHRALGKAGDLTGYHWGLTRKRAILGWEAGKA
HHHHHHHHCCCCCEEEEECHHHCCCCCCCCCEECCCCCCCEEECCCCCCC
>Mature Secondary Structure
MNVATSIPTDITPEGTDYDTVTRVIAMLTEDYREQPSLESLARRLGQSPTQLQKVFTRWA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHC
GLSPKAFLQAITLDHAKRLLRQEDLPLLETSIEIGLSGPSRLHDLFVTHEAMSPGEWKAR
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHEEECCCCHHHHHHHHHHHCCCCCCCCEEC
GAGLTIRYGFHPSPFGTALVMVTERGLAGLAFADSGEEHASFEDMASRWPNAIYLEDSAA
CCCEEEEECCCCCCCCCEEEEEECCCCCEEEECCCCCHHCCHHHHHHHCCCEEEEECCCH
TARYAARIFDPDRWSAEEPLRIFLIGSDFQVRVWQTLLKIPLGKATTYSKIAENIGQPTA
HHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
SRAVGAAVGRNPISFVVPCHRALGKAGDLTGYHWGLTRKRAILGWEAGKA
HHHHHHHHCCCCCEEEEECHHHCCCCCCCCCEECCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]