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The map label for this gene is leuB [H]

Identifier: 150398370

GI number: 150398370

Start: 3343230

End: 3344342

Strand: Direct

Name: leuB [H]

Synonym: Smed_3178

Alternate gene names: 150398370

Gene position: 3343230-3344342 (Clockwise)

Preceding gene: 150398368

Following gene: 150398371

Centisome position: 88.4

GC content: 63.88

Gene sequence:

>1113_bases
ATGACTGTGCGCAATCTCTTCCTTCTGCCCGGCGACGGCATCGGCCCGGAAGCCATGGCGGAAGTCCGCAAAATCATCGC
TTACATGAATGCCGAGCGCGACGCGGGCTTCGTGACGGATGAGGGGCTTGTGGGTGGTTCCGCCTATGACGCTCACGGCG
CTGCGATCTCCGAAGGGGACATGGCGAAGGCGCTTGCCGCCGACGCGGTGCTTTTCGGCGCCGTCGGCGGACCGAAATGG
GATGCGGTGCCATATGAGGTGCGGCCGGAAGCCGGCCTCCTGCGCCTGCGCAAGGACCTCGAGCTTTTCGCCAACCTGCG
GCCGGCCATCTGCTATCCGGCGCTGGCCAATGCGTCATCGTTGAAGCCGGAACTGGTGGAAGGCCTCGATATCCTCATCG
TGCGCGAGCTGACGGGCGGCGTCTATTTCGGCGAGCCGAAGGAGATCGTCGATCTCGGCAACGGTCAGAAACGCGGCATC
GATACGCAGGTCTACGACACCTATGAGATCGAGCGCATCGCCGGCGTCGCCTTCGAGCTTGCCCGCACCCGCAACAACCG
CGTCTGCTCGATGGAAAAGCGCAATGTGATGAAATCGGGCGTCCTGTGGAACCAGGTCGTGACCGAGACGCACAAGGCGA
AATATTCCGACGTTCAGCTCGAGCACATGCTGGCGGATGCGGGCGGGATGCAGCTCGTGCGGCAGCCCAAGCAGTTCGAC
GTGATCGTCACCGACAACCTCTTCGGCGACATGCTGTCCGACGTCGCCGCCATGCTGACCGGTTCGCTGGGCATGCTGCC
ATCCGCTTCTCTCGGAGCGCCCGACGCCAAGTCCGGCAAGCGCAAGGCGCTCTATGAACCGGTGCATGGCTCGGCACCCG
ACATCGCCGGCAAGGGCATCGCCAACCCGATCGCCATGATCGCCTCCTTCGCCATGTGCCTGCGCTACTCCTTCAACCTC
GTGACGGAAGCCGACAATCTGGAGAAGGCGATCGCCAACGTTCTCGACCAGGGCATCCGCACCGGCGACATCATGGCGGA
CGGCTGCCGCCAGGTCGGTACCACCGAGATGGGCGAGGCGATCCTCGCCGAGTTCAAGACCCTTTCGGCCTGA

Upstream 100 bases:

>100_bases
CTCGATACGAAGTTGCGCTAAGAACGGCGCCTGCAGCACCGTGCGTCCCGTTGGACGCACGCAGGTCGCTGCAGCAGTTT
TCCCGCTGGAAGGACTATCC

Downstream 100 bases:

>100_bases
CGGCTGACCCGGTTAGGCCCGGATGAATGGCACCTCCGGCTTTGCCGGGGGTGCTTTGCGTTTTGCGGGGTCTTGATTTT
ACCGGCCGCGTCTTCACATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTVRNLFLLPGDGIGPEAMAEVRKIIAYMNAERDAGFVTDEGLVGGSAYDAHGAAISEGDMAKALAADAVLFGAVGGPKW
DAVPYEVRPEAGLLRLRKDLELFANLRPAICYPALANASSLKPELVEGLDILIVRELTGGVYFGEPKEIVDLGNGQKRGI
DTQVYDTYEIERIAGVAFELARTRNNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKSGKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFNL
VTEADNLEKAIANVLDQGIRTGDIMADGCRQVGTTEMGEAILAEFKTLSA

Sequences:

>Translated_370_residues
MTVRNLFLLPGDGIGPEAMAEVRKIIAYMNAERDAGFVTDEGLVGGSAYDAHGAAISEGDMAKALAADAVLFGAVGGPKW
DAVPYEVRPEAGLLRLRKDLELFANLRPAICYPALANASSLKPELVEGLDILIVRELTGGVYFGEPKEIVDLGNGQKRGI
DTQVYDTYEIERIAGVAFELARTRNNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKSGKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFNL
VTEADNLEKAIANVLDQGIRTGDIMADGCRQVGTTEMGEAILAEFKTLSA
>Mature_369_residues
TVRNLFLLPGDGIGPEAMAEVRKIIAYMNAERDAGFVTDEGLVGGSAYDAHGAAISEGDMAKALAADAVLFGAVGGPKWD
AVPYEVRPEAGLLRLRKDLELFANLRPAICYPALANASSLKPELVEGLDILIVRELTGGVYFGEPKEIVDLGNGQKRGID
TQVYDTYEIERIAGVAFELARTRNNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFDV
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKSGKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFNLV
TEADNLEKAIANVLDQGIRTGDIMADGCRQVGTTEMGEAILAEFKTLSA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=367, Percent_Identity=31.6076294277929, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI28178821, Length=318, Percent_Identity=28.6163522012579, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28178816, Length=318, Percent_Identity=28.6163522012579, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4758582, Length=347, Percent_Identity=30.2593659942363, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=30.1492537313433, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=36, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=49.1666666666667, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=33.0623306233062, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787381, Length=308, Percent_Identity=26.6233766233766, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=32.2766570605187, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=349, Percent_Identity=30.945558739255, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=365, Percent_Identity=29.8630136986301, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=41.5549597855228, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=34.3839541547278, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=31.5934065934066, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324291, Length=375, Percent_Identity=27.2, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=33.9080459770115, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=33.9080459770115, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24661184, Length=351, Percent_Identity=31.3390313390313, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078637, Length=370, Percent_Identity=30, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078635, Length=344, Percent_Identity=29.9418604651163, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078633, Length=370, Percent_Identity=30, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650122, Length=370, Percent_Identity=30, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078639, Length=370, Percent_Identity=30, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=28.3236994219653, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=28.3236994219653, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20130355, Length=351, Percent_Identity=25.6410256410256, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39630; Mature: 39499

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRNLFLLPGDGIGPEAMAEVRKIIAYMNAERDAGFVTDEGLVGGSAYDAHGAAISEGD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
MAKALAADAVLFGAVGGPKWDAVPYEVRPEAGLLRLRKDLELFANLRPAICYPALANASS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
LKPELVEGLDILIVRELTGGVYFGEPKEIVDLGNGQKRGIDTQVYDTYEIERIAGVAFEL
CCHHHHCCCCEEEEEECCCCEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ARTRNNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
HHCCCCCEECHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCEE
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKSGKRKALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ANPIAMIASFAMCLRYSFNLVTEADNLEKAIANVLDQGIRTGDIMADGCRQVGTTEMGEA
CHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHH
ILAEFKTLSA
HHHHHHHCCC
>Mature Secondary Structure 
TVRNLFLLPGDGIGPEAMAEVRKIIAYMNAERDAGFVTDEGLVGGSAYDAHGAAISEGD
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
MAKALAADAVLFGAVGGPKWDAVPYEVRPEAGLLRLRKDLELFANLRPAICYPALANASS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
LKPELVEGLDILIVRELTGGVYFGEPKEIVDLGNGQKRGIDTQVYDTYEIERIAGVAFEL
CCHHHHCCCCEEEEEECCCCEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ARTRNNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
HHCCCCCEECHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCEE
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKSGKRKALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ANPIAMIASFAMCLRYSFNLVTEADNLEKAIANVLDQGIRTGDIMADGCRQVGTTEMGEA
CHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHH
ILAEFKTLSA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA