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The map label for this gene is argG2 [H]

Identifier: 150398257

GI number: 150398257

Start: 3211578

End: 3212795

Strand: Reverse

Name: argG2 [H]

Synonym: Smed_3063

Alternate gene names: 150398257

Gene position: 3212795-3211578 (Counterclockwise)

Preceding gene: 150398259

Following gene: 150398254

Centisome position: 84.95

GC content: 60.67

Gene sequence:

>1218_bases
ATGGCATCGCATAAAGACGTGAAAAAGGTCGTTCTCGCTTATTCCGGCGGACTCGACACCTCTATCATCCTCAAATGGCT
CCAGACGGAACTGGGCGCCGAAGTGGTGACCTTCACCGCCGACCTCGGCCAGGGCGAGGAATTGGAGCCGGCCCGCAAAA
AAGCGGAGATGCTCGGCATCAAGGAGATCTATATCGAGGACGTCCGCGAGGAATTCGTGAAGGACTTCGTCTTCCCGATG
TTCCGGGCCAATGCCGTTTATGAAGGCGTCTACCTGCTCGGCACCTCGATTGCCCGTCCGCTGATCTCCAAGCACCTCAT
CGACATCGCCAGGAAGACCGGCGCCGACGCGATCGCCCATGGCGCGACCGGCAAGGGCAACGACCAGGTCCGCTTCGAAC
TCTCGGCTTACGCGCTCAATCCGGACATCAAGATTATCGCGCCCTGGCGCGACTGGTCGTTCAAGAGCCGCACCGATCTG
CTCGAATTCGCCGAAAAGCACCAGATTCCGGTCGCCAAGGACAAGAAGGGGGAGGCGCCCTTCTCCGTCGATGCCAACCT
TCTTCACTCTTCCTCGGAGGGGAAAGTTCTGGAGGATCCGGCACAGGAAGCGCCCGAATATGTGCATATGCGCACCATTT
CGCCGGAAGCAGCGCCCGACAAGGCGACGATCATCAAGGTCGGCTTCGAGCGCGGCGATGCTGTTTCAATCGACGGTGTG
CGCATGTCGGCGGCGACGCTTCTTGCAAAGCTCAACGAATATGGCCGCGATAACGGCATCGGTCGCCTCGATCTCGTGGA
AAACCGCTTCGTCGGCATGAAGTCGCGCGGCGTCTACGAAACCCCCGGCGGCACGATCCTGCTTTCGGCTCATCGCGCCA
TCGAGAGCATCACGCTCGACCGCGGCGCGGCGCATCTTAAGGACGAGCTGATGCCGCGCTACGCCGAGCTCATCTATTAC
GGTTTCTGGTTCTCGCCCGAACGCGAGATGCTGCAGGCCGCGATCGACAAGAGCCAGGAGCATGTGGAAGGCGAGGTGAC
ACTCAAGCTCTACAAGGGCAACGTCATGGTCGTCGGCCGGGAAAGCGGCAAGTCGCTCTATTCCGACAAGCTCGTCACCT
TCGAAGACGACCAGGGCGCCTACGACCAGAAGGATGCTGCAGGCTTCATCAAGCTCAACGCGCTGCGCCTGCGCACGCTC
GCGGCGCGCAATCGCTGA

Upstream 100 bases:

>100_bases
GCGATCCCGGCCTGCTAACGCTTGTATCCCGTCACGTTCTGGCTAAAGTGCCGCAGATTTTCGTGCGGCCTCGCCGCCCC
CGAGCAGACGGACAAGAACC

Downstream 100 bases:

>100_bases
GACGATTGAGCGGGAGCTGCGGTGGAGCAATTCGCCGCGGCTCGTCTCCGCTCATTCCGCCGCCTGAACGATAGGATCTT
GCTCGGCCGTCGGCGCGGCC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase 2 [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MASHKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIYIEDVREEFVKDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDL
LEFAEKHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATIIKVGFERGDAVSIDGV
RMSAATLLAKLNEYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLSAHRAIESITLDRGAAHLKDELMPRYAELIYY
GFWFSPEREMLQAAIDKSQEHVEGEVTLKLYKGNVMVVGRESGKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTL
AARNR

Sequences:

>Translated_405_residues
MASHKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIYIEDVREEFVKDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDL
LEFAEKHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATIIKVGFERGDAVSIDGV
RMSAATLLAKLNEYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLSAHRAIESITLDRGAAHLKDELMPRYAELIYY
GFWFSPEREMLQAAIDKSQEHVEGEVTLKLYKGNVMVVGRESGKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTL
AARNR
>Mature_404_residues
ASHKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIYIEDVREEFVKDFVFPMF
RANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDLL
EFAEKHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATIIKVGFERGDAVSIDGVR
MSAATLLAKLNEYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLSAHRAIESITLDRGAAHLKDELMPRYAELIYYG
FWFSPEREMLQAAIDKSQEHVEGEVTLKLYKGNVMVVGRESGKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLA
ARNR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=49.4974874371859, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=49.4974874371859, Blast_Score=382, Evalue=1e-106,
Organism=Escherichia coli, GI1789563, Length=418, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=43.7037037037037, Blast_Score=329, Evalue=6e-91,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=47.7611940298507, Blast_Score=354, Evalue=7e-98,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44938; Mature: 44807

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASHKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIYIEDVREEFVKDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDLLEFAEKHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATIIKVGFERGDAVSIDGV
CCCCHHHEECCCCCCEECCHHHHCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
RMSAATLLAKLNEYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLSAHRAIESITLD
HHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCEECCCCEEEEECHHHHHHHHHC
RGAAHLKDELMPRYAELIYYGFWFSPEREMLQAAIDKSQEHVEGEVTLKLYKGNVMVVGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCHHHCCCEEEEEEEECCEEEEEC
ESGKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLAARNR
CCCCCHHHCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCC
>Mature Secondary Structure 
ASHKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIYIEDVREEFVKDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIDIARKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTDLLEFAEKHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAQEAPEYVHMRTISPEAAPDKATIIKVGFERGDAVSIDGV
CCCCHHHEECCCCCCEECCHHHHCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
RMSAATLLAKLNEYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLSAHRAIESITLD
HHHHHHHHHHHHHHCCCCCCEEEEEHHCCCCCCCCCCCEECCCCEEEEECHHHHHHHHHC
RGAAHLKDELMPRYAELIYYGFWFSPEREMLQAAIDKSQEHVEGEVTLKLYKGNVMVVGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCHHHCCCEEEEEEEECCEEEEEC
ESGKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLAARNR
CCCCCHHHCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA