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The map label for this gene is leuC [H]

Identifier: 150398253

GI number: 150398253

Start: 3208066

End: 3209475

Strand: Reverse

Name: leuC [H]

Synonym: Smed_3059

Alternate gene names: 150398253

Gene position: 3209475-3208066 (Counterclockwise)

Preceding gene: 150398254

Following gene: 150398252

Centisome position: 84.86

GC content: 62.84

Gene sequence:

>1410_bases
ATGAGCGCACCGCGTACTCTCTACGACAAGATCTGGGACGATCATCTGGTCGACAGCCAGGCCGACGGAACCTGTCTTCT
CTACATCGACCGTCACCTCGTTCATGAGGTGACGAGTCCTCAGGCCTTCGAGGGCCTGCGCATTGCCGGCCGCAAGGTTC
GAGCCCCGGAAAAGACGCTCGCAGTCGTCGATCACAACGTCCCGACGTCGCCGGACCGCCATCTCGGCATCAAGAACGAG
GAAAGCCGCATTCAGGTAGAGGCACTCGCAAGGAATGCGGCGGATTTCGGCGTCGAATATTATTCGGAGAGCGACAAGCG
CCAGGGGATCGTTCACATCGTCGGCCCCGAGCAGGGTTTCACCCTGCCGGGCATGACGATCGTCTGTGGCGACAGCCACA
CTTCGACGCATGGCGCCTTCGGTGCCCTGGCGCACGGCATTGGCACGTCCGAGGTCGAGCACGTTCTGGCCACGCAGACG
CTGATCCAGAAAAAGGCAAAGAACATGCTGGTGCGCGTAGACGGCCAGTTGCCGCCGGGCGTCACCGCCAAGGACATCAT
CCTTGCCATCATCGGTGAAATCGGAACGGCGGGCGGCACCGGCCACGTTATCGAATTCGCCGGCGAAGCCATCCGCTCGC
TGTCGATGGAAGGCCGGATGACCGTCTGCAACATGACGATCGAAGGCGGCGCCCGTGCGGGCCTGATCGCTCCGGACGAA
ACGACTTTCGCTTATATCGAGGACAGGCCGCGCGCACCGAAGGGTGAGGCTTGGGATATGGCGGTCGAATACTGGAAGAC
GCTGCATACGGACGAAGGCGCCCATTACGACCGCGTCGTCGTTCTCGATGCGGCGAACCTGCCGCCGATCGTCTCCTGGG
GCTCCTCGCCGGAAGACGTCGTTTCCGTCCAGGGCGTCGTTCCCAATCCGGACGAGATCCCGGACGAGACGAAGCGCACC
TCGAAGTGGCGCGCGCTCGACTATATGGGGCTGAAGCCCGGCACCAGGATCACCGATATCGCGATCGACCGGGTGTTCAT
CGGCTCCTGCACCAATGGCCGTATCGAGGACCTGCGCGCCGTCGCCAAGGTCGTCGAAGGCCGCAAGGTCGCCCCGACGG
TTTCGGCGATGATCGTTCCCGGTTCCGGCCTCGTCAAGGAACAGGCGGAAGTGGAAGGCCTGGACAAGATCTTCAGGGAA
GCGGGTTTCGACTGGCGCGAGCCCGGCTGCTCCATGTGCCTGGCGATGAACGACGACCGGCTGAAGCCCGGCGAACGTTG
TGCCTCCACATCGAACCGCAACTTCGAAGGCCGCCAGGGCTTCAAAGGGCGCACGCATCTGCTTTCTCCGGCAATGGCGG
CTGCAGCGGCGATCGCCGGGCATTTTGTCGACATTCGCGAATGGAAATAG

Upstream 100 bases:

>100_bases
CTCTTACCAGCCGCCTATCCTGATCGCTCGATCAGCAGTTTGCCGGCCGCCTTGGTGCCGGCCCCATTCCACATTCCTTA
TTCTGATGGACATACAAGCC

Downstream 100 bases:

>100_bases
CCGGTTTGAGCTTCCGCCATCTGGCCGCCCTTCGGGGCGGCTTTTTTTACACTCCAGGCAGTGCGACAATGCGCGGCCAT
CCCCGCGAAAACTGCCCGTG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAPRTLYDKIWDDHLVDSQADGTCLLYIDRHLVHEVTSPQAFEGLRIAGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNE
ESRIQVEALARNAADFGVEYYSESDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGQLPPGVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
TTFAYIEDRPRAPKGEAWDMAVEYWKTLHTDEGAHYDRVVVLDAANLPPIVSWGSSPEDVVSVQGVVPNPDEIPDETKRT
SKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRAVAKVVEGRKVAPTVSAMIVPGSGLVKEQAEVEGLDKIFRE
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLLSPAMAAAAAIAGHFVDIREWK

Sequences:

>Translated_469_residues
MSAPRTLYDKIWDDHLVDSQADGTCLLYIDRHLVHEVTSPQAFEGLRIAGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNE
ESRIQVEALARNAADFGVEYYSESDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGQLPPGVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
TTFAYIEDRPRAPKGEAWDMAVEYWKTLHTDEGAHYDRVVVLDAANLPPIVSWGSSPEDVVSVQGVVPNPDEIPDETKRT
SKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRAVAKVVEGRKVAPTVSAMIVPGSGLVKEQAEVEGLDKIFRE
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLLSPAMAAAAAIAGHFVDIREWK
>Mature_468_residues
SAPRTLYDKIWDDHLVDSQADGTCLLYIDRHLVHEVTSPQAFEGLRIAGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNEE
SRIQVEALARNAADFGVEYYSESDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMLVRVDGQLPPGVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDET
TFAYIEDRPRAPKGEAWDMAVEYWKTLHTDEGAHYDRVVVLDAANLPPIVSWGSSPEDVVSVQGVVPNPDEIPDETKRTS
KWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRAVAKVVEGRKVAPTVSAMIVPGSGLVKEQAEVEGLDKIFREA
GFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLLSPAMAAAAAIAGHFVDIREWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=380, Percent_Identity=28.1578947368421, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI8659555, Length=381, Percent_Identity=27.0341207349081, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=26.0526315789474, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=66.4516129032258, Blast_Score=626, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=27.3480662983425, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI87081781, Length=355, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=382, Percent_Identity=29.0575916230367, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=382, Percent_Identity=29.0575916230367, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=29.3333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=27.4074074074074, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=65.0423728813559, Blast_Score=622, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320440, Length=427, Percent_Identity=27.4004683840749, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=29.3963254593176, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=378, Percent_Identity=27.7777777777778, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI281365315, Length=478, Percent_Identity=25.5230125523013, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=478, Percent_Identity=25.5230125523013, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=480, Percent_Identity=25.8333333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.4397905759162, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137564, Length=377, Percent_Identity=28.6472148541114, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=27.5590551181102, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50932; Mature: 50801

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRTLYDKIWDDHLVDSQADGTCLLYIDRHLVHEVTSPQAFEGLRIAGRKVRAPEKTL
CCCCHHHHHHHCCHHCCCCCCCCEEEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCEE
AVVDHNVPTSPDRHLGIKNEESRIQVEALARNAADFGVEYYSESDKRQGIVHIVGPEQGF
EEEECCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGQLPPG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFAYIEDRPRAPKGEAWDMAVEYWKTLHTDEGAHYDRVVVLDAANLPPIVSWGSSPEDV
CEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCHHHE
VSVQGVVPNPDEIPDETKRTSKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRA
EEEECCCCCCCCCCCHHHHHCHHEEEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
VAKVVEGRKVAPTVSAMIVPGSGLVKEQAEVEGLDKIFREAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLLSPAMAAAAAIAGHFVDIREWK
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
SAPRTLYDKIWDDHLVDSQADGTCLLYIDRHLVHEVTSPQAFEGLRIAGRKVRAPEKTL
CCCHHHHHHHCCHHCCCCCCCCEEEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCEE
AVVDHNVPTSPDRHLGIKNEESRIQVEALARNAADFGVEYYSESDKRQGIVHIVGPEQGF
EEEECCCCCCCCCCCCCCCCCHHEEHHHHHHHHHHHCHHHHCCCCCCCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGQLPPG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFAYIEDRPRAPKGEAWDMAVEYWKTLHTDEGAHYDRVVVLDAANLPPIVSWGSSPEDV
CEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCHHHE
VSVQGVVPNPDEIPDETKRTSKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRA
EEEECCCCCCCCCCCHHHHHCHHEEEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
VAKVVEGRKVAPTVSAMIVPGSGLVKEQAEVEGLDKIFREAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLLSPAMAAAAAIAGHFVDIREWK
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA