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The map label for this gene is amtB [H]

Identifier: 150398245

GI number: 150398245

Start: 3199165

End: 3200520

Strand: Direct

Name: amtB [H]

Synonym: Smed_3051

Alternate gene names: 150398245

Gene position: 3199165-3200520 (Clockwise)

Preceding gene: 150398244

Following gene: 150398246

Centisome position: 84.59

GC content: 62.24

Gene sequence:

>1356_bases
ATGTCGTCATTCATTCCTTCCACCTCTCTTCGGCGCGTGGCCGCGACGGCTTCCGCCATGCTCGCGCCCGTCGTTGCATT
TGCTCAGGAAGCGGCACCCGCCGCGGCCGCAACACCGGTGCCGGACAAGGCCGATACCGCGTTCATGTTCCTTTCGACGC
TGCTCGTCCTGTTCATGCTGATTCCGGGCCTTGCCCTCTTTTACGGCGGCCTCGTACGAGCCAAGAACATGCTGTCGGTG
CTGATGCAGTGCACGGTCATCGGCGCCGCCATGATGATCGTCTGGGTCGTTTACGGTTATTCATTTGCATTCGGCGGCTC
GACCAGCGCCTATTTCGGCGGCTTCGCCAAGCTTTTCCTCGCGGGCGTGACCGTCGATAGCACGGCTGCGACCTTCACCG
ACGGTGTCGTCATCCCGGAATATCTCTTCATGCTGTTCCAGATGACCTTCGCCGCGATCACCCCGGCACTCATCGTCGGT
GCCTTTGCCGAGCGTATCAAGTTCTCGGCCGCAATCCTCTTCTCCATTCTCTGGGCGACATTCGTCTATTTTCCAATCGC
GCATATGGTCTGGGACGCCAACGGGCTGCTCTTCGGCATGGGCGCGCTTGATTTTGCCGGCGGCACCGTCGTCCATATCA
ATGCCGGCGTCGCAGGCCTCGTGGGTGCATTCATGGTGGGCAAGCGCATCGGCTATGGCAGGGATATGATGGCCCCGCAT
TCGATGACCCTGACGCTTGTCGGAGCGGCCATGCTGTGGTTCGGCTGGTTCGGCTTCAATGCCGGCTCCAACCTCGAAGC
ATCCGGCGGTGCGGTTCTGGCTACCGTCAACACCTTCCTCGCAACGGCTGCCGCAGTTCTTTCGTGGTCGCTCGTCGAAA
CGCTGACGCGTGGCAAGGCGTCGATGCTCGGCGCCGCCTCGGGCATGATTGCCGGCCTCGTCGCCGTCACCCCGGCTGCC
GGTATCGCCGGCCCGATGGGTGCCATCATTATGGGCCTGATCGTCTCGCCGCTCTGCTACTTCTTCGTCTCGGTCATCAA
GAACAAGTTCCACTATGACGACACAGCAGACGTCTTCGGCGTTCATGGCGTCGGCGGTTTCTTCGGCGCGATCGCTACCG
GCATCTTCGCCTCCTCGTCGCTCGGCGGCATCGGCTATGCCGACGGCGTGACCATGGCCGGCCAGCTCATGACCCAGCTC
ACGGCAGTCGCCATCACGATCGTCTGGTGCGGCGTCGTTTCCGTCATCCTTTACAAGGTGGTCGATGCGCTTGTCGGCCT
GCGCGTCAGCGTGGAAGCGGAGCGGGAAGGCCTCGACCTCTCCTCGCATGGCGAAGCCGCTTATCACGCGAGCTGA

Upstream 100 bases:

>100_bases
CAAGATTTTCGTCTACTCGATTGACCACGCCGTGCGCATCCGCACCGGCGAGACCGACGCGGAAGCGTTGTAAACCACGC
CGTTCAGGGAGCTATTACCG

Downstream 100 bases:

>100_bases
GCCGCGTCGGCAAACGACGCCGCCAACAGTCCCGTCGCGGTCCGCATCCTGCGGATCGCCCTTCGCCCGGATCGCAGGAT
CCGGGCACTTTTTTGCGCGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSSFIPSTSLRRVAATASAMLAPVVAFAQEAAPAAAATPVPDKADTAFMFLSTLLVLFMLIPGLALFYGGLVRAKNMLSV
LMQCTVIGAAMMIVWVVYGYSFAFGGSTSAYFGGFAKLFLAGVTVDSTAATFTDGVVIPEYLFMLFQMTFAAITPALIVG
AFAERIKFSAAILFSILWATFVYFPIAHMVWDANGLLFGMGALDFAGGTVVHINAGVAGLVGAFMVGKRIGYGRDMMAPH
SMTLTLVGAAMLWFGWFGFNAGSNLEASGGAVLATVNTFLATAAAVLSWSLVETLTRGKASMLGAASGMIAGLVAVTPAA
GIAGPMGAIIMGLIVSPLCYFFVSVIKNKFHYDDTADVFGVHGVGGFFGAIATGIFASSSLGGIGYADGVTMAGQLMTQL
TAVAITIVWCGVVSVILYKVVDALVGLRVSVEAEREGLDLSSHGEAAYHAS

Sequences:

>Translated_451_residues
MSSFIPSTSLRRVAATASAMLAPVVAFAQEAAPAAAATPVPDKADTAFMFLSTLLVLFMLIPGLALFYGGLVRAKNMLSV
LMQCTVIGAAMMIVWVVYGYSFAFGGSTSAYFGGFAKLFLAGVTVDSTAATFTDGVVIPEYLFMLFQMTFAAITPALIVG
AFAERIKFSAAILFSILWATFVYFPIAHMVWDANGLLFGMGALDFAGGTVVHINAGVAGLVGAFMVGKRIGYGRDMMAPH
SMTLTLVGAAMLWFGWFGFNAGSNLEASGGAVLATVNTFLATAAAVLSWSLVETLTRGKASMLGAASGMIAGLVAVTPAA
GIAGPMGAIIMGLIVSPLCYFFVSVIKNKFHYDDTADVFGVHGVGGFFGAIATGIFASSSLGGIGYADGVTMAGQLMTQL
TAVAITIVWCGVVSVILYKVVDALVGLRVSVEAEREGLDLSSHGEAAYHAS
>Mature_450_residues
SSFIPSTSLRRVAATASAMLAPVVAFAQEAAPAAAATPVPDKADTAFMFLSTLLVLFMLIPGLALFYGGLVRAKNMLSVL
MQCTVIGAAMMIVWVVYGYSFAFGGSTSAYFGGFAKLFLAGVTVDSTAATFTDGVVIPEYLFMLFQMTFAAITPALIVGA
FAERIKFSAAILFSILWATFVYFPIAHMVWDANGLLFGMGALDFAGGTVVHINAGVAGLVGAFMVGKRIGYGRDMMAPHS
MTLTLVGAAMLWFGWFGFNAGSNLEASGGAVLATVNTFLATAAAVLSWSLVETLTRGKASMLGAASGMIAGLVAVTPAAG
IAGPMGAIIMGLIVSPLCYFFVSVIKNKFHYDDTADVFGVHGVGGFFGAIATGIFASSSLGGIGYADGVTMAGQLMTQLT
AVAITIVWCGVVSVILYKVVDALVGLRVSVEAEREGLDLSSHGEAAYHAS

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=415, Percent_Identity=55.6626506024096, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17550182, Length=446, Percent_Identity=28.9237668161435, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=439, Percent_Identity=29.3849658314351, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=439, Percent_Identity=27.5626423690205, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=26.9662921348315, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=35.0119904076739, Blast_Score=216, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=33.2552693208431, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=432, Percent_Identity=32.4074074074074, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=27.536231884058, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46657; Mature: 46526

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCEEEEEHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSFIPSTSLRRVAATASAMLAPVVAFAQEAAPAAAATPVPDKADTAFMFLSTLLVLFML
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH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HHHHHCCEEEEEHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]